LOCUS       NZ_CP071715             6773 bp    DNA     circular CON 05-JUL-2023
DEFINITION  Escherichia coli strain EC20017429 plasmid pL4, complete sequence.
ACCESSION   NZ_CP071715
VERSION     NZ_CP071715.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18140782
            Assembly: GCF_023921205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6773)
  AUTHORS   Yousfi,K., Walker,M., Bernaquez,I. and Bekal,S.
  TITLE     First report and genomic analysis of multidrug resistance Shiga
            toxin-producing E. coli producing (O118:H2) harboring ESBL, mph(A)
            and blaLAP-2 in human in Canada
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6773)
  AUTHORS   Bekal,S. and Yousfi,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2021) Bacteriologie, Laboratoire de Sante Publique
            du Quebece, 20045 Ch Ste-Marie, SainteAnne-de-Bellevue, Quebec H9X
            3Y3, Canada
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Unicycler v. Galaxy version 0.4.8.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 150.0x
            Sequencing Technology             :: Nanopore Technology
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 07/05/2023 03:54:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,022
            CDSs (total)                      :: 5,893
            Genes (coding)                    :: 5,596
            CDSs (with protein)               :: 5,596
            Genes (RNA)                       :: 129
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 100
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 297
            CDSs (without protein)            :: 297
            Pseudo Genes (ambiguous residues) :: 0 of 297
            Pseudo Genes (frameshifted)       :: 121 of 297
            Pseudo Genes (incomplete)         :: 170 of 297
            Pseudo Genes (internal stop)      :: 62 of 297
            Pseudo Genes (multiple problems)  :: 52 of 297
            Pseudo Genes (short protein)      :: 4 of 297
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071715.1.
            Bacteria and source DNA available from Laboratoire de Sante
            publique du Quebec, Canada.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC20017429"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pL4"
                     /geo_loc_name="Canada: Quebec"
                     /lat_lon="46.81 N 71.21 W"
                     /collection_date="2017-04-29"
                     /collected_by="LSPQ"
     gene            complement(357..500)
                     /locus_tag="JQM62_RS29945"
                     /old_locus_tag="JQM62_29705"
     CDS             complement(357..500)
                     /locus_tag="JQM62_RS29945"
                     /old_locus_tag="JQM62_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724568.1"
                     /translation="MKKITWIILLLLAAIILAACQANYIHDVQGGTVSPSSSAELTGLA
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(598..927)
                     /locus_tag="JQM62_RS29950"
                     /old_locus_tag="JQM62_29710"
     CDS             complement(598..927)
                     /locus_tag="JQM62_RS29950"
                     /old_locus_tag="JQM62_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="WP_000182536.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKKG
                     LWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTAFI
                     ITLKM"
                     /name="colicin transporter"
     gene            complement(917..2587)
                     /locus_tag="JQM62_RS29955"
                     /old_locus_tag="JQM62_29715"
     CDS             complement(917..2587)
                     /locus_tag="JQM62_RS29955"
                     /old_locus_tag="JQM62_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_000012962.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADD
                     ITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTA
                     SIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHN
                     AVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARN
                     QERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQMAGLKAQR
                     AQTDVNNKQAAFDAAAKEKADADAALSTAMESRKKKEDNKRDAEGKLNDELAKNKGKIP
                     GLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGRNDPATVYGS
                     PGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(2820..5039)
                     /gene="mobQ"
                     /locus_tag="JQM62_RS29960"
                     /old_locus_tag="JQM62_29720"
     CDS             complement(2820..5039)
                     /gene="mobQ"
                     /locus_tag="JQM62_RS29960"
                     /old_locus_tag="JQM62_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_100019840.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEVLVGFVRQEDGLRPV
                     LALPSPDYSHLMVSTITENGVCLAGYGEAINHDADTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            5218..5493
                     /locus_tag="JQM62_RS29965"
                     /old_locus_tag="JQM62_29725"
     CDS             5218..5493
                     /locus_tag="JQM62_RS29965"
                     /old_locus_tag="JQM62_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039023505.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADMNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(5700..6635)
                     /locus_tag="JQM62_RS29970"
                     /old_locus_tag="JQM62_29730"
     CDS             complement(5700..6635)
                     /locus_tag="JQM62_RS29970"
                     /old_locus_tag="JQM62_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_032488643.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAIERALCEKLGADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASA
                     RRSIDKHYGMGRNCYLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVATTHTPEIQAARGRKGGIAKGAGYEDKRSTAL
                     EMLVNGCTQKAIAEMLGVSDRTIRNWKSGK"
                     /name="replication initiation protein"
     BGC             1..6773
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4682..5040
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5041..5214
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(5701..6635)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.026"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2824..5039)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.241"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tcccgaaaac ccgatccatt taccataact cccgttgtac gtacccctta aaacactctc
       61 aatcgctttc tgacgcaaaa aaaacaaaaa gacaaggaaa caccagggta aacataaaaa
      121 atgcgtcaga atcgttttta gcgcgttgca taactatgca cgaaagcgat gcacgatcgg
      181 tttcgtgcgt tctgaacacc gaacggctca aaacgacaag ttttctcccc ctggatggaa
      241 agttctgcac ggggatttta gatcccatct cccacatgga atttttgtga cacttcaaaa
      301 aaaagcgcgt cagaatcagc ctgacacgct ttgttcttgt caaagaggat ttcgggttac
      361 tgcgttgcta atccggtcag ttcagctgag gatgacgggg atacagtccc gccctgaaca
      421 tcgtggatat agtttgcctg acatgcagca agaataatgg ctgcaagaag caataaaata
      481 atccatgtta tttttttcat actgttcctt gtatatatgg actcaggcat tcatccataa
      541 aaacctgaaa tccaaaggtt gaaatcatcc atgaaacata aaattctata acactattta
      601 catctttaac gtgataatga aagcagtgta tccattctca atatcatctt taatattttc
      661 aatagccaat ctattatttt cagggatttt tgatttgtat tcctccggca tatcatatgt
      721 ccatgatgct gaactatcta tcctgaatcc aggattcagc caatgcccac catcaatgcc
      781 caaaatccat aagccttttt tcgctgcttc attacaaact tctattgcag cttttggtga
      841 taacttcata gaagcatttc cttttaattc aaaaaaggca tcaccactat catataacac
      901 cgtatgttca aataatttat tggtcatttt tatttcccca ttgaattctc gaatcgtcca
      961 cccaacccgg atctgtctta tcactaatct gagtaacctc accagtacga tcattgacaa
     1021 caacatattt accaggagaa ccatacactg ttgctggatc atttcgacct aaatagtctg
     1081 gaggtgtttt cttcggacta cgcttatcaa aagatgtacc agttgcaccg ttagatacgg
     1141 tatttttgat atcatcctct gtccatcctc tttctggcat ttgtccccta attttctgat
     1201 ctatttttag acctggtatc tttcctttat tttttgctaa ctcatcattt agtttgcctt
     1261 cagcatctct tttattatcc tctttcttct tcctgctttc catcgcagta cttaatgcag
     1321 catcagcatc tgccttctct tttgcagcag catcaaatgc agcctgctta ttatttacat
     1381 ccgtctgcgc ccgctgagct ttaagcccgg ccatttgcca cattctgtga ccgccagcca
     1441 ttgggtcatg tgcaaatcgt tcaaattgtt ttatttcagc tattgcatca gcaagagttt
     1501 tattcgctgc atcaagttcg cttttacgcg aattataaac ctgaacagct ttagcctgtc
     1561 gctcctgatt tctggcaaca tcttcatttg cctgattcag ctctgcacgc gcgcgttcat
     1621 aatttcgctc agccgcttca accggatgcg tagcatccca ttcctgctga cggcgatttt
     1681 cttcatcctg acgttgtttt acctggtcag ggctaagaac atcactcact gaaacatata
     1741 cggcattatg accgctgtcc ttcgggaatc gaataactgc atccctggta ttacccccct
     1801 gagtaaatcc tgccgggcga acatccttat cagtattatt tgtaacacct gggcttaatg
     1861 tctgtactgc tggcgtactg ttattaactg aaatattcag aacaggtgca cctggaattg
     1921 atgccgtaaa aacacctgga cgttcggtag gttttgcatc aaccaccgga acactcatcg
     1981 gaacacctga aacaaccgaa atattctgtc gttcgtcttt tacatcatca acaacacgaa
     2041 catttacgtt tactgttgcc ttatcgagag gtaatgaact gacaggtgat tcagtaatat
     2101 catctgcggg taatgacgtc acaatctttg acatcatatt ggggtcatct ttcgctattt
     2161 gtgatggcaa tacaccatat aaagccaccc cccaaagacc aaatttaaac ggtcctttca
     2221 gggcagccat aatatcagca atagctgccg ataatgctcc cgctgaaata ctgaccgcca
     2281 gaccgccagc tcctggagtg gaaagtgccg gaaaaccaaa tgccactggc gcagctactg
     2341 ctgacagatt accgcctgtt cccgaaccac caccggaatt accattcccc ccgccattac
     2401 catgaccgga accaccaccc cagtgaatgc cgctaccgga accaccaccc cacgggttat
     2461 tttccgaact ccatccggag ccatcagaag caccaccacc tacaccaagc ccggtcgggc
     2521 caccattaat gttaccactt gtgctatgcg cgcccgtgtt atggccgcgt ccatcgccac
     2581 cgctcataaa aattccctct tttaagcgtt aaaacaatta attacatgat taatactgta
     2641 cataaaacca ctggttttat gtacagatca aaaagtaccc gtcatcgttt tttctgtcaa
     2701 gttcaggcgg aaaatttttt agaaatcagg agggataaca cttagaaaac gcagagcggg
     2761 ccacaaaaaa ttttgtggcc cgtgatgtgt tggcggggtc cggggtgcag ccccggatat
     2821 cacatactca tgccccggaa gtgcccgggg cggcgttgtt gttgcctgtg ctgttcgtcc
     2881 cgcttccact catgccaggt ctgattaaaa ccgagttgac ggaagagata atccggcatc
     2941 tcttccggtt tgtggacagc agccaaaagc ctgtcatgat ggcctttgag ccggaactgc
     3001 atcaggtgcg gttccggtgg gtacggagta tccgcatcat ggtttatagc ttctccgtaa
     3061 cctgccaggc ataccccgtt ctccgtgatg gtgctgacca tcagatgact gtaatccggc
     3121 gacggaagcg caagaacggg acgcagcccg tcctcctggc gcacaaatcc gacaagcact
     3181 tcctccggcc ctttgcacaa cagcagattc agcgtcctgc tgtttcgctc tgtcgaaaat
     3241 acaacgggca tgagttgctc cggtgccgca taagcggtga cgtccacccg gtgcgcagac
     3301 ggcagcggag aggctacggg cctgcggtcg tcgctgaaat aaaaattatc cggccagtga
     3361 tgcggcagcg ccggaaaacc gtaagagggc cacccctggc gcatctgttc cagtacatgc
     3421 cggaccgtcg caccggaata cattctcaca cgctcaggcc gcgccatcgt ctcatgagat
     3481 tcaggcattc cctcacgtat atccgagacc atcacagccc cgcctggcga caggtaaaca
     3541 ttcaccgggt catgaacact ctgggggtaa tgcgccagct catcacccca taccgtgaat
     3601 tctccggctc tgttactcag catcatgtag cgggttatcc tgcccgttcc cggctctctg
     3661 gcacgcccca gctccgtaat aaccccgact aatgccgttt cacggctcag acggtggtta
     3721 tcatgacgtt tcagccgcgc ctcatgctcc tgttccttcc ggcgcgcttc cgcttcggcc
     3781 tcctccctgc gccgctccgc ttcccgtgct gacagttttt ctttcagctc tgccattccc
     3841 ggcgcaatcc acgtattcca ctgcgtttca aaggcctgac gacgggcggg aatgtcccgt
     3901 tctttcacgg ccctgtaggt gcggcgaaga gattcaatct gtttcccgag tttccagaaa
     3961 cggagttgtc ttttgcggta ttccggttca gcccatgtca tcaggggaat ctctgacaga
     4021 agggacgcga cagggcgtgc ttttttgagt ccggtcatct ccttttcgag aatggcctgc
     4081 tctttcagca gcgcattccc ctcacgttcg atgtcgttca ccttcccgct catcgccagg
     4141 aactccgggg tcatctccca cgcgttcccg cattcctgcg gcagcgtggc aatgaattcc
     4201 cgcagggtat ccgctgaggc attcgcagcg ggaatggtca ggttaagggc ttccgctgca
     4261 cgacgtgcag catcgctgag ttcccgctta cgctgcgctt cctgaaccgt cctgagagca
     4321 agctgcgtgc gggaaacctg tatttccagg ttaaggctgt taatcagacg gttctgctca
     4381 ccgcgttctg tttccattcc ccgtttttcc atcgcacagg cagcctttcc gaggtgaagg
     4441 cccggcgctt tctccagtcc ctgacgctcg tatgaacgat ggtctatctc ttcctggtag
     4501 ccggcgttcg caagcgctct gttcgcatgg tcagcccatg atgcgcgcca cgtctcggcg
     4561 agtgcccggt cgttccagtc cctgaccttt cctgcgaatc ccgtttcccc gacagctctc
     4621 gtggtcagca tgatgtgcgc atggggattg gtccggtcca tgtgatggaa cgccacatcg
     4681 gcaatcatgc cccgactgac aaagttttcc cggacaaaag cgagaacggt ctcccgtgcg
     4741 gcctcgcggg aaatctcacg gggaatggca agttcaatct cccttgccag ctgggagttc
     4801 cggcgctgtt cggctttctc gaccgcattc cagagaacgg aacggtcacc acaccaggac
     4861 ggtgcattcg caggggtgag aatgaccgca tcatcaacgc cgcgtttacg ggtgtaatcg
     4921 tggattaatc cggtacggtc atcgggtatg acagagccgg aacggtaggc agcagaagca
     4981 acagcactgt agccgttttt tcgcgaaatg attttcatgc tgagatgata aatcgccatt
     5041 tctgccgtaa tgcctttttg ccgttttctt ttagcgtcgc agaccgcaca caatcgaaac
     5101 gaagtggaga ttgtataagt gcgcacttcg tgatgtgccg tgcattatgc cagttcagtt
     5161 ttgcaactac cagtgacgcg ctaaaatgcg atttttcacc gcgcacggag tcacgaaatg
     5221 agcagaacac tcgaacagaa gattgccgaa gctgaggcca gattacagcg tctgaaggcg
     5281 aagagccgga gcctggacac ggcgcaaaaa gtcattgtgg gggctgccat gctggcgagg
     5341 gtacggaggc cggaagaagc gcagctgcga gcgttcctgc tccagttcct gcgaaaggaa
     5401 gtcacaagac aggccgatat gaaccggcta caaccgctga ttaacgagct ggagaagcta
     5461 cccagaccac ctgcgaaacc gcagaaccac taagccccgg cgcgtaagtg ccacatgcac
     5521 caccggacgc ctttgcgctt cctcgctcat tcgctcgcta cgctcggtca tgagactgcg
     5581 gcgagcgctg ccaccgcaat cacgaggaaa ccagcgccat tgctggacgg aaccggaaaa
     5641 accacaggaa caccaagagc tcaaaaaagc gccgaaaagg cgctgttatc tgatataggc
     5701 tattttccgc ttttccagtt tcgaatagtg cgatcggaaa cacctaacat ttcagctata
     5761 gccttttgag tacacccatt caccaacatt tccagcgcgg tgctgcgctt atcctcatag
     5821 ccagcccctt tggctatacc acctttacgg cctcgggcag cttgaatttc cggcgtgtgc
     5881 gttgtcgcca catactgtgc gaaagtttcc ggcgtaaagt tcctgtgcgt gtatttcgct
     5941 atgctcttgc caatagcccg acattcagcc ggagaaagcg gaacgggaag tgatgcgttg
     6001 tacatttcga cacgctggat cacggcatca agccattgtg agaatacagg ccagccctga
     6061 cgaatcgccc tgtaagccca tttgcgcgtc atttcgaaca ggtagcagtt tcgccccatc
     6121 ccgtaatgtt tatcgatgct acggcgcgct gaggcgctca aatcgagata atcagccagt
     6181 tcatcgaggg tataggcttc ctcgcgccac tccatcacca gccattccag gtggaacgga
     6241 tttttgcaaa tcagtccgct gtagttcaca tctgcaccaa gtttctcaca cagcgcacgc
     6301 tctatcgcgg cagcgtactt cagtgcctta accgacgcat caggcgcggt tctcacggcg
     6361 atgttgagtg catagagcag gtgagcatga ccgttcacgg gattttttac ggtgatgttc
     6421 ggcgcggggg cgttccggtc gctccagtca atcgcagccc cctcgcggtc aacgtcaaaa
     6481 accagccaga actgcgcatg aggctggttc tgctggatgt accgcgcaag aagcgcacgc
     6541 cctttgccgg agatgcgaag accaaaagcg agatcatccg tgtgataggg acggtggggt
     6601 aaattttctt cgaagtattg aagcgcggcg ctcatgtgtt acgattcctt ccaggttatc
     6661 gaccgacctc gcccctgtat tctcgtgaca atcttggcgg agagacacga atttcagggg
     6721 cgtttgtttt tcagtcgctt acactacacc caacggcaaa tcctgtcgaa cct
//
