LOCUS       NZ_CP071398             1943 bp    DNA     circular CON 01-APR-2024
DEFINITION  Klebsiella michiganensis strain Kmfe267 plasmid pKmfe267-5, complete
            sequence.
ACCESSION   NZ_CP071398
VERSION     NZ_CP071398.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18138829
            Assembly: GCF_017348855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Hu,L.M., Zhang,X.T. and Liu,L.G.
  TITLE     Genomic characterization of a pan-drug resistant Klebsiella
            michiganensis
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 1943)
  AUTHORS   Zhang,X.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2021) Clinical Research institute, University of
            South China, 35 Liberation Ave., Hengyang, Hunan 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017348855.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 04:57:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,095
            CDSs (total)                      :: 5,976
            Genes (coding)                    :: 5,844
            CDSs (with protein)               :: 5,844
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 52 of 132
            Pseudo Genes (incomplete)         :: 99 of 132
            Pseudo Genes (internal stop)      :: 27 of 132
            Pseudo Genes (multiple problems)  :: 37 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071398.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="Kmfe267"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="pKmfe267-5"
                     /geo_loc_name="China:Hengyang"
                     /lat_lon="26.89 N 112.57 E"
                     /collection_date="2019-05-14"
                     /collected_by="XiaoTuan Zhang"
     gene            join(1872..1943,1..42)
                     /locus_tag="J1F62_RS30735"
                     /old_locus_tag="J1F62_30315"
     CDS             join(1872..1943,1..42)
                     /locus_tag="J1F62_RS30735"
                     /old_locus_tag="J1F62_30315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001517211.1"
                     /translation="MHEPPVQSDCCACSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     Typing          1498..1943
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.357"
                     /coverage="85.0"
                     /evalue="7.770000000000004e-156"
ORIGIN
        1 agacatgcaa aagcgccact ggcagcagcc actggtaatt gatttagagg agttagtctt
       61 gaagttatgc gccggttaag gctaaactga aaggacaagt tttggtgact gtgctcctcc
      121 aagccagtta cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccttg
      181 cagggcggtt ttttcttttt caagcgaaag actacccgct gaattaaata atctcaagta
      241 gcaacatgaa atctaacgag tcagtgtcga gctagaagat ggtgaggaca gagtaatact
      301 tgaattagtg cagctagatg attcaacggt attaatcatt ctatcaaggc gaaccataca
      361 tttctcaacg acaagcccac ctctaccatc actgctaatg ttggtcacaa atggacctgc
      421 ttggcttgag cagccagcaa gaattactga tgacagaaaa accaatgaga gaattacctt
      481 tttcataaaa gccccagaaa atagagaagt aggaataatc ttatctttta atttgttaat
      541 caacataaac gatatttacc ataacatccc ttgcccgcac cgctgaaaac gcctcagagc
      601 cattttgagg cgaacgggcc gtttccggca cctttcccag caccaacccc tgaaaaacgc
      661 gcctgatgat ttctgcgcat ctgcatggct atgcacgaac atgcctggcc ggaattttag
      721 cggctaaaat cctgcgaact gctcaaaagg ctcgctgaac cccatcgata acggtgaacg
      781 ggtgcacagt ttactgtgcg gtcgtgaggc gttgagcaac gagctttgcg aggcggtagg
      841 ggttctgaga gccagctgca ggctcaggag aaaacattct gttttcgggg cagataccgc
      901 aaatgacgtc attttgcggc gtctgcccta tcctgcatcg caacgtctaa aggatgtgag
      961 cacaaacgcc agcactttgc gaatcctgcc ctcgatctga acaaaagcaa aatttcgtac
     1021 gtacgaaatt cagggtatca ggctgcgcct gatgaaatgt ttttggtgcc ggcggtactc
     1081 caaaaacaca tcttgtccga cagttttgtg gtgccggcga agcggcggtg gccacaaaac
     1141 atttcctgtc cccatcgatt cattcgagaa acataagagg cgaagaaggg gtcagtttgg
     1201 agatcggaaa tttttgtacg ttaagcgcat tatttaacca atcttggttc gtaaggggtt
     1261 gattttgcgt cagtggctat cgcttttaag ttgtcgctct tgcagtatcg catttaggtt
     1321 atccgattaa ggtcaaacct ctgaaaatgc cgtatagcgc gggagtacac cctattgcta
     1381 taggtaagtc tgttcaaaaa acaggcttac cgtacaataa ttctctatat ccaaactgac
     1441 ccctggcagg tgaaaaatgt agctgtaggg cgtaagtgaa aaatgcagga gtcgatgaaa
     1501 ttacgcctcc tcgctcactc ggtcgctacg ctccggacgt gagactgcgg cgggcgttac
     1561 cggctcagaa gtaaaggagt aggctagtag caatcaaatt gggaaaggaa gagaaaagga
     1621 agcggcgttg ccgtttttcc ataggctccg cccccctgac aagcatcaca aaatctgacg
     1681 ctcaaatcag tggcggcgaa acccgacagg actataaaga tcccaggcgt ttccccctgg
     1741 tagctccctc gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt
     1801 atggccgcgt ttatctcatt ccacgcctga cactcggttc cgggaaggca gttcgctcca
     1861 agctggactg tatgcacgaa ccccccgttc agtccgactg ctgcgcctgt tccggtaact
     1921 atcgacttga gtccaacccg gaa
//
