LOCUS       NZ_CP071397             2772 bp    DNA     circular CON 01-APR-2024
DEFINITION  Klebsiella michiganensis strain Kmfe267 plasmid pKmfe267-4, complete
            sequence.
ACCESSION   NZ_CP071397
VERSION     NZ_CP071397.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18138829
            Assembly: GCF_017348855.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella michiganensis
  ORGANISM  Klebsiella michiganensis
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Hu,L.M., Zhang,X.T., Zeng,X., Chen,Y., Liu,L. and Li,G.Q.
  TITLE     Genomic Characterization of a Carbapenemase-Producing, Extensively
            Drug-Resistant Klebsiella michiganensis Strain from a Renal Abscess
            Patient
  JOURNAL   Microbiol Resour Announc 11 (12), e0082522 (2022)
   PUBMED   36321904
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Hu,L.M., Zhang,X.T. and Liu,L.G.
  TITLE     Genomic characterization of a pan-drug resistant Klebsiella
            michiganensis
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 2772)
  AUTHORS   Zhang,X.T.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAR-2021) Clinical Research institute, University of
            South China, 35 Liberation Ave., Hengyang, Hunan 421001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017348855.1-RS_2024_04_01
            Annotation Date                   :: 04/01/2024 04:57:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,095
            CDSs (total)                      :: 5,976
            Genes (coding)                    :: 5,844
            CDSs (with protein)               :: 5,844
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 132
            CDSs (without protein)            :: 132
            Pseudo Genes (ambiguous residues) :: 0 of 132
            Pseudo Genes (frameshifted)       :: 52 of 132
            Pseudo Genes (incomplete)         :: 99 of 132
            Pseudo Genes (internal stop)      :: 27 of 132
            Pseudo Genes (multiple problems)  :: 37 of 132
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071397.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Klebsiella michiganensis"
                     /mol_type="genomic DNA"
                     /strain="Kmfe267"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:1134687"
                     /plasmid="pKmfe267-4"
                     /geo_loc_name="China:Hengyang"
                     /lat_lon="26.89 N 112.57 E"
                     /collection_date="2019-05-14"
                     /collected_by="XiaoTuan Zhang"
     gene            complement(851..2035)
                     /locus_tag="J1F62_RS30310"
                     /old_locus_tag="J1F62_30310"
     CDS             complement(851..2035)
                     /locus_tag="J1F62_RS30310"
                     /old_locus_tag="J1F62_30310"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_207172972.1"
                     /translation="MELTKKQKQIAEARLMIAHQQSIDSGTFIEIQIDKMIKGIIGKVK
                     TNANDASKEQYRKHYRSAIERHPDASFHELLMQAGTASSFDKRRTAFRFCITEEIQNLR
                     RKSEKAMRSKDYEAGKKLIQEAFKLAIKFEDEFLSDNRILFNDVKSQEGFKKVSFSKKK
                     GLKKAPDPDVVLNGLELKKNLYDRHSLAFAVISVFGIRPAEMQKGVKLKFIDGMIFAQV
                     KGAKVSDDKGHKYRAIGVSVRDGNKCDQVIIEAIYKNGGEVLATQTKSDYDSLRKYFNN
                     HYNGTSLYTYRHQVASDLKKAGVSKTQIAEVMGHRTTESQVHYGYARSSRGGRSMVAKG
                     SNEVISSPDIKDYLKPKPAPAGGGGTGSRIAKAKNNLSQGVQASYPKLTPRGTK"
                     /name="site-specific integrase"
ORIGIN
        1 tcgaaggatt tctaccaaac tctagatttt tcatggagtt gaaacttcgc cgatcttact
       61 ctctctcaaa taaaagatcg agtattacta actctgatca agccttcggc ctgtgctacg
      121 ccataaggga taaaggatgt tcagccccca gaaaaagggc gcttcaccca aagtttcgcc
      181 tcctcgaatt catcacgaat tcaagttgac gatcctttgg taaagcttct tttttgagct
      241 tcacccaaag agcagcgact tagagctgaa ggcagtttcc caccaactgg caccctgttg
      301 cacccccgct gtcgccacat agcactactt gcagaatcct aaccccattt atggctgggc
      361 tttctcatag ctggcctgaa tccttaccaa gcgccttagt gactcgttcc cggtctggtc
      421 ttttgcaaca ctccccgttt agaccaacac caacacctac ccgaacaatc acatgcatat
      481 gcctccatat gcggctccac aggtagatcc cgatacaatg ggttattaaa tgtattggtg
      541 cttaaggact tgaaaaggct agattttttt tggcacttgt aaggaaatag gtttgacttt
      601 tacgcggatg taggatagat ttgatctaca tatcgtaatc acgttgtctg aaccaacttg
      661 gttacaagac ctaaaaagtc tgcaaacttt ttaagtccct caaagccagc ctattcggtt
      721 ggctttgttg tatctgtcac ctgttataac cgaaatcgct tacacgtcaa tagtctatca
      781 aaaaaattga agtcaaaatc attgacggct aacccatcaa aaaacgagtt tttgacggct
      841 tacccgcctc ctacttggtt ccgcgaggag tcagctttgg gtaactggcc tgaaccccct
      901 gagataaatt atttttggcc tttgcgatcc tgcttccagt tccaccacca cctgcaggag
      961 ccggttttgg tttcagatag tctttgatgt ccgggctact gattacctca ttagacccct
     1021 tcgctaccat agaacgcccg ccccttgagc ttctggcgta tccataatga acctgtgact
     1081 cagtagttct atgccccata acctcagcta tttgagtttt tgagacaccc gcttttttta
     1141 aatcgcttgc tacttggtgg cggtatgtat aaagagaagt cccgttataa tggttattaa
     1201 aatacttgcg taaactgtca taatcgcttt ttgtttgagt agctaaaact tcaccaccat
     1261 ttttataaat ggcctctata ataacctgat cgcatttatt accatcacga acagaaacac
     1321 ctattgctct atacttatga cctttatcat ccgatacctt tgcaccttta acctgcgcaa
     1381 aaatcatacc atctataaac tttagcttta cgcctttttg catttctgcc gggcgtatac
     1441 caaatactga aataacagca aaagctaatg aatgcctatc atataagttc tttttcaact
     1501 ccaaaccatt taaaacaaca tcaggatcag gagccttttt aagtcctttt ttcttactga
     1561 aagaaacttt tttaaacccc tcttggcttt ttacatcatt aaatagaatt ctattatcag
     1621 ataaaaattc atcttcaaat ttaatagcaa gtttaaatgc ttcttggatt agtttttttc
     1681 ctgcttcata atctttagaa cgcatagcct tttcagattt acgtctcaag ttctggatct
     1741 cttcagtgat gcaaaacctg aaggctgtac gcctcttatc aaagctagag gccgtccccg
     1801 cttgcatcag aagctcatga aaggatgcat ccgggtgacg ttctatagca gatcggtagt
     1861 gcttcctgta ctgctctttt gaggcatcgt tggcattcgt tttcactttc ccgatgatac
     1921 ctttaatcat tttatcaatt tgtatttcga tgaatgtacc gctatcaatt gattgttgat
     1981 gtgctatcat caatcttgct tcagctattt gtttttgctt tttggttagc tccatgtcag
     2041 atctcctttt tatttttaca ttggtgttgt ctgtattttt attcttccta ttgcgaaaag
     2101 tcgctcgctc ctaatcgcaa aagtcagaat aaaaaaaggt gttttgctac ccgcaacttt
     2161 cttatttttc tgtcgaaaaa acgaaagaca cacctacatt attgcgaacg aaagggccgt
     2221 tctcaataat gttaaaatct tacgttccca ataatgtata tgtaattaat aatcaattat
     2281 ttttggattg tccatgtacc tttaaaaaat tattttctag cgcgtcagcg agtgggattt
     2341 attcaaaaaa aaaggccggg ggtttatccg gccttataat attttaggtt ttatcttcta
     2401 tgaaatgtta ttcggtttgc ataaatacct ttcagtacca agaaacctac acccataaaa
     2461 acaaatccta aaatcatgga tgatcctgac tgtgtataca ttgctaacaa tgcaccgccg
     2521 accatcccca ctaaccacac caagaagact aaccaaccgg gcggcgttcc atacttcacc
     2581 gtagcaaggc acgttcgaca aacatgcact cccttcgtca gctcagtatc acagtacgca
     2641 cagtggagaa cttcttttgt gttgttttcc atatcaaaaa atccaataaa gtttgatagt
     2701 tacagaccat ttttaaattg atctgacctt cgcctattct tcgtaaagtg ctcgaaaaaa
     2761 aagcgtcatt ta
//
