LOCUS       NZ_CP071270             2495 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Citrobacter freundii strain MEI002 plasmid p5_Col(pHAD28), complete
            sequence.
ACCESSION   NZ_CP071270
VERSION     NZ_CP071270.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18062620
            Assembly: GCF_023612475.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Brilhante,M., Rohrbach,H., Endimiani,A., Schuller,S. and Perreten,V.
  TITLE     Promiscuous carbapenemase-containing plasmid spreads in three
            different Enterobacteriaceae species in the outdoor areas of a
            veterinary clinic
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Brilhante,M. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2021) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern, Bern 3012,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023612475.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 06:22:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,826
            CDSs (total)                      :: 4,706
            Genes (coding)                    :: 4,639
            CDSs (with protein)               :: 4,639
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 21 of 67
            Pseudo Genes (incomplete)         :: 42 of 67
            Pseudo Genes (internal stop)      :: 13 of 67
            Pseudo Genes (multiple problems)  :: 8 of 67
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071270.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="MEI002"
                     /isolation_source="Outdoors environment of a veterinary
                     clinic"
                     /db_xref="taxon:546"
                     /plasmid="p5_Col(pHAD28)"
                     /geo_loc_name="Switzerland:Bern"
                     /collection_date="2019-08"
                     /genotype="ST22"
     gene            1180..1398
                     /locus_tag="J0G25_RS24105"
                     /old_locus_tag="J0G25_24030"
     CDS             1180..1398
                     /locus_tag="J0G25_RS24105"
                     /old_locus_tag="J0G25_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(1689..1874)
                     /locus_tag="J0G25_RS24110"
                     /old_locus_tag="J0G25_24035"
     CDS             complement(1689..1874)
                     /locus_tag="J0G25_RS24110"
                     /old_locus_tag="J0G25_24035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2240..2467)
                     /locus_tag="J0G25_RS24115"
                     /old_locus_tag="J0G25_24040"
     CDS             complement(2240..2467)
                     /locus_tag="J0G25_RS24115"
                     /old_locus_tag="J0G25_24040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1555..1642
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(388..916)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 tggtcactaa ccatctaact ataggtcatt ttttatgtga aaagaggctt gatggtgggc
       61 tgtatatcga gttaggcgcg aggtgctatg gtcaaagtgt ggtgtcaggc ggggaggagg
      121 cttatatgag atgtaatccg gccgatacat ataccaagat gatttaagta ttcctctttg
      181 tgaggttgga tgtctaattt ataaaggatc ttcttgagat cctttttttc cgcgcgtaat
      241 ctcttgccct gtaaacgaaa aaaccaccct ggcaggtggt ttttcgaagg ttaggtaatc
      301 ctggcagatc ccctaaccgt ggtaacagtc ttgtgcgaga catgtcacca aatttgtcct
      361 ttcagtgtag cctcactaag gccgccactt caagaactct tgagacatct ctcgcacatc
      421 ctgtttgcca atggccgttg ccaatggcga ttagtcgtgt ctttccgggt tggactcaag
      481 ttgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
      541 catcctggag cgaactgcct tcccggaact gagtgtcagg cgtggaatga gaaaccgcgg
      601 ccataacagc ggagtgacac cggtaaaccg aaaggcagga atgcggggga gcacgaggga
      661 gccaccaggg ggaaacgcct ggtatcttta agccgcatcg ggtttcgcca ccactgattt
      721 gagcgtcaga ttctgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttggc
      781 gcggccttat gctttcttcg ttaagtatct tcctggcatc ccccaggaaa tttctgatcc
      841 atccgtaagc ccgtcccgct cgccgcagcc gaacgaccga gcggagcgag tcagtgagcg
      901 aggaagcgga atatattctg tatcacattt tctcctgacg cgttttcttt cactttctgc
      961 gcctgtctta tgtggcatta atgctatgtg ttactgccat gctacatctt aagccagtat
     1021 acactccgct agtgctccgt gactggtccg gcgctgcgcc cggaacccgc ctgtaccggt
     1081 tcagcagccg ttccggcctg actgcaattt tttttttttc atccctgccc gctaccctgt
     1141 aaacctttct tctgcgttgc cgttaacagt aggtaagcca tgtttggttt ttcttccgga
     1201 gccctgatga ctcttagtga tgatgagcga cacctgcttg tgtccgttgt ttcggtctgg
     1261 ctacggcgtg ccggagggga tgccggagcc atgatgctgg atgcataccg gcagattttg
     1321 tctgaaaccg aaccggccgt aaggactgtc atgctcgaat ttcttgaatc cgttcgcatt
     1381 cattacattt cgtcctgagc attttctgta tttctgtcca tacttaaacg aggcggccgt
     1441 cacggacggt ggtcgtcgtt ccatgatgct gttatggacg agtgagagta atctaatcta
     1501 acttgattga gttccgggcg tgccgcccgg ttttttttgc tgtttcccgg tgtccgttcc
     1561 gggacaggat gtgcatttgt aataccgcgc acgcgccggc atcacaaata ccctggtcag
     1621 ggctgcgccc cgacaccccg ccatcgcctc acggccgcgc actgaattgc gcacccgttc
     1681 accgattttc acgcattttc ctcgcctatc tgtgcgttca gcatggcgta aagcgcttcg
     1741 gcctgatcgt gcaggtgctc acagcagccg gcagcctcat ccagatccat ctgctcgagc
     1801 cgctcaagga ggagaagtgt ctgtgcgcgg ataaatttgg ccatattgag ggcggcggat
     1861 tcctgtctgg tcactggtga ttttccccgt ctggtgatga tttttgcgtg aaaagagcat
     1921 ggcgtcattc tggtgcatag tcatgcatgc cgttaaaatt taacaggcgc gtttctgacg
     1981 ggttccgggg tggtttgttg tggtttttgt catggtttcg tcaggaacgc gctgaacgcg
     2041 tctcaggcgg tgcgggcaac ggatgttatg gtaaattctt gggggcttga ttataaaata
     2101 ccgcacttcc tcttatgtct tgtgcttttt agacaaccct gaaagctttt agctttggtg
     2161 atgcaatttg ctgggatttt ggcggagaac cacaggtaaa gaaaaaggcc acattagcgg
     2221 cctttttcgg agaagatgct cagtgcggaa tttcggagac ttctttaacg tcggttttat
     2281 tgatctgctg tttgacacca ttcgcgtctg tatagccaag caggcctgag tctgattctt
     2341 taggtttgcc gtcgctgaca atagtgcgac cgtcattggt gtgtatggca tagcttgtgc
     2401 gcgtgcagcc ggtgagggct gaaagcgcaa ctgccgctgc gaacactgaa acaaaaatct
     2461 ttttcaaagc cagctccttt tatccatatc agttt
//
