LOCUS       NZ_CP071258             4369 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae strain MEI006 plasmid p6_Col440I, complete
            sequence.
ACCESSION   NZ_CP071258
VERSION     NZ_CP071258.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18062624
            Assembly: GCF_023612395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Brilhante,M., Rohrbach,H., Endimiani,A., Schuller,S. and Perreten,V.
  TITLE     Promiscuous carbapenemase-containing plasmid spreads in three
            different Enterobacteriaceae species in the outdoor areas of a
            veterinary clinic
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Brilhante,M. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2021) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern, Bern 3012,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023612395.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:05:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,676
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,432
            CDSs (with protein)               :: 5,432
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 54 of 125
            Pseudo Genes (incomplete)         :: 76 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 21 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071258.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="MEI006"
                     /isolation_source="Outdoors environment of a veterinary
                     clinic"
                     /db_xref="taxon:573"
                     /plasmid="p6_Col440I"
                     /geo_loc_name="Switzerland:Bern"
                     /collection_date="2019-08"
                     /genotype="ST3251"
     gene            join(4123..4369,1..731)
                     /locus_tag="J0G26_RS28245"
                     /old_locus_tag="J0G26_28185"
     CDS             join(4123..4369,1..731)
                     /locus_tag="J0G26_RS28245"
                     /old_locus_tag="J0G26_28185"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4435 domain-containing protein"
                     /protein_id="WP_064147929.1"
                     /translation="MADFEDIFIGEEYLEPYCAMIGSKGLVFVEGLKDISFWEGMLDNN
                     DDLKYSINYASHEGTRGKTVLMKFSHLANKFALFAIDSDFDYICPNNSEKSKNINSNKH
                     IIQTLVYSKESITLHHLVLSDCIKKIKIKDEITFPIKSYFESYSNEIYEVLTRFLFLKE
                     NGKAVNDSDFHKKITPIEPKINKNYFLENDPLITLREQKESLVSEYTSNELAEEDFIRF
                     RDELSIKGLNENSAAYFINGHFLEESVVMPLINELIRTLKANELEKIKIECKKNKKSIE
                     SNRNETFKYIDNELGIYKLLHDTKIKYQTSLSIMVKEANKRSLD"
                     /name="DUF4435 domain-containing protein"
     gene            complement(1730..2131)
                     /locus_tag="J0G26_RS28250"
                     /old_locus_tag="J0G26_28190"
     CDS             complement(1730..2131)
                     /locus_tag="J0G26_RS28250"
                     /old_locus_tag="J0G26_28190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_023287146.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIRAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2131..2322)
                     /locus_tag="J0G26_RS28255"
                     /old_locus_tag="J0G26_28195"
     CDS             complement(2131..2322)
                     /locus_tag="J0G26_RS28255"
                     /old_locus_tag="J0G26_28195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_085843021.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSLRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2738..2851
                     /locus_tag="J0G26_RS28430"
     CDS             2738..2851
                     /locus_tag="J0G26_RS28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049026061.1"
                     /translation="MHEPPVQSDCCALSGNCHLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3153..4130
                     /locus_tag="J0G26_RS28260"
                     /old_locus_tag="J0G26_28200"
     CDS             3153..4130
                     /locus_tag="J0G26_RS28260"
                     /old_locus_tag="J0G26_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723132.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_085843020.1"
                     /translation="MSILVKNIKAKGLFGSKDINWDLKDVNVLVGKNGAGKSTLLRSLH
                     SMLKQEGSEELTRSELMKVTLSEGGTITHTINKLSQDTDELLSTLMGFSSSIKKGKEAQ
                     KEKIKLEKLIDKIKAKKAEIKEIGFSKIEVSEDIRAKTKIKSELISTVNMSANSILPLT
                     GSDGEVTTILDMEIKNEINKLKKIINNKESECNELICKLEKSINDLFEESRKRVSFSSG
                     ELEIERIDKKEELYLSNLSSGERQVIYIMLKAANTSKEETVLLMDEPEISLHLTWQEKL
                     INSILYVNEKCQLIIVTHSPAIVMKGWMGAFVDIKNIQEETING"
                     /name="ATP-binding protein"
     Typing          2444..2851
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.11"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgactttga ttacatttgc cctaacaata gcgaaaaatc taaaaatata aattcaaata
       61 aacacataat tcaaacatta gtttattcaa aagaaagcat aacccttcat catctggtcc
      121 ttagtgattg cataaaaaaa ataaaaataa aagacgaaat aacattcccg ataaagagtt
      181 attttgagtc atactcaaat gaaatatatg aagtactgac aagattctta tttttaaaag
      241 aaaatggcaa ggctgtcaat gattctgatt ttcacaaaaa gataacaccc atagaaccaa
      301 aaataaacaa gaattatttt ttagaaaacg accctctaat tacattaaga gagcaaaaag
      361 aatctctagt atctgaatac acaagcaatg aattagcaga agaggatttc attaggttta
      421 gagatgaatt atcaatcaaa gggcttaacg aaaacagcgc agcttacttc ataaacggtc
      481 attttcttga agaatcagta gtcatgccat taataaatga attaataaga acacttaaag
      541 caaatgaact tgaaaaaata aaaatagaat gcaaaaaaaa caaaaaatcg atagaatcca
      601 acagaaatga aacctttaaa tatattgata atgaacttgg catttataag ttgctacacg
      661 atacaaaaat aaaataccaa acatctttat cgataatggt aaaagaagct aacaaaagat
      721 cattagatta gaaagacaca ccaccaaaat atcaataata aaaaatttgg tggtgctctt
      781 aactttcaaa aaaaatacgc atcaccatat caccccctaa caaaacaaaa aacatatata
      841 tatcaattag ttacaaaaac acattaccgc atccgccact aaaaaggatg cgcgatacaa
      901 aattaccagc gagcagctca ataactaagt caacatgacg gcgaacaggg cgccggaatg
      961 tggccaactt cgcagaataa cctattagac cctatgagga ttttaggctc cattatttaa
     1021 cataaagcta tgattgtacg caccgctaaa acggcctcag ggcgtttctg acgggaaacc
     1081 ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa cgcgcaggct ctgtttttac
     1141 ggcatgcatg agtatgcacg caagtgcata gcgcgaaaat tcacgccatt cagccagggt
     1201 aagtcctgtt tccggcgttc ttcccatccg aaaacggtga acggggacgc aatttattgc
     1261 gtggccgtga agcgttgccg gcgcagccgg aaaaccggtt ttcagcacat tcaggaatcc
     1321 ggttcgtcga tggccggcaa tcacgttatg gcaagtcacc gaacgactca ccacacgatt
     1381 caccggcgac tcttcactag agtcgtaccc agagtcgcca cgtctaaaaa acaaccaaac
     1441 ggggagtcgg gtctagagtc gggtcgttat taactcattg tttttatagt catgagtcgt
     1501 taccgactct atgacgactc gtcatagagt cggatctata taaatcaaat ttaaaaaacg
     1561 aaatttaacc aacttaaact actgatatta aatacaaatc cgcaaaatgt taattttgcg
     1621 tggggtgtgg gtatttttag tggtgaggag cgttcagaga tctgacgatt tttgggaagg
     1681 ttcaggaact ccccgggatg gccggagagg aaaactgaag catgacgggt caggattttt
     1741 tccagaattt ccaccatggc gccggggatt cagttaattt tgcctggtga ttttcaagaa
     1801 ggagcatggc ctgtttcagg ctgtcaatat gcgaatcctt ggacaaaagt aaggtgttca
     1861 ggtgttcaat ctcctgtttc agatgacgaa tcacctgttc actctggtca gcagaggctg
     1921 tatcacgctg ttcagagacc tgtccagaat ggtgttcacc cttcggttgc tcaaagggac
     1981 caaagacacg caaaagctct gaagtatcta ccatttttgc attgcgttca ccgctacagg
     2041 tggacaggtc acctgctcta attaacctat ggagtgttgt tctactctta cccgtcagtc
     2101 gggcagcctc actaatagac actctggcca tcagttacca tcctgtaatt catccagtct
     2161 caaggacagt gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaaggtcggc
     2221 ctcaacatcg agatcaagct cgttaagctt ctccagcagg agcagagact gattctggat
     2281 gaatttagcc atgttcagcg ctgttttctg ttgtttcgtc ataagaagcc tcagagcgtc
     2341 agtgctcagt gtagggatgt tctcttttcc gcttcctcgc tcactcagtc gctacgctcc
     2401 tgccgttcgg ctgcggcgag cggcaggagc atgcgtagaa acgctcgaga aaaccgagca
     2461 atcacataac ggaaagaagg aaaggccgct cagcggccgt ttttccatag gctccgcccc
     2521 cctgacaagc atcacagaaa ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
     2581 taaagatatc aggcgtttcc ccctgatggc tccctcgtgc gctctcctgt tcctgccttt
     2641 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacatt
     2701 cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     2761 cgactgctgc gccttatccg gtaactgtca tcttgagtcc aacccggaaa gacatgcaaa
     2821 agcgccactg gcagcagcca ctggtaactg atttagagga gatagtcttg aagttatgcg
     2881 ccggttaagg ctaaactaaa cgaacagttt tggttactgc gctcctccaa gccagttacc
     2941 tcggttcaaa gagcgccaac tcagagaacc ttcgaaaaaa ccgcccgcca aggcggtttt
     3001 ttcgtttaaa gaggaggata cagctatcaa aatgcgatca caagaggcct gcggccacat
     3061 ataggcaaat caatctatag atttactaca gacagcttta aatatagaat caataattat
     3121 tatctacata attacatgag gtaattataa gaatgagcat attagtaaaa aacataaaag
     3181 caaaaggtct atttgggagc aaagacatca attgggattt aaaagatgtc aatgttcttg
     3241 ttggtaaaaa tggtgcagga aaatcaacat tactgagatc tcttcactcc atgttaaaac
     3301 aagaaggaag cgaggagcta actagaagtg agttaatgaa agtaacctta agtgaaggtg
     3361 gaacaattac tcatactatt aacaaactca gtcaagacac tgatgaatta cttagcacac
     3421 taatgggctt ctctagctct ataaagaaag gaaaggaggc ccaaaaagaa aaaataaaac
     3481 ttgaaaagtt aatagacaaa ataaaagcca aaaaagcaga aataaaagaa ataggattta
     3541 gcaaaataga agttagtgaa gacatcagag caaagacaaa gatcaaatct gagttgatat
     3601 caactgtaaa tatgagtgca aactctatct tacctttaac tggtagtgat ggagaggtca
     3661 caactatact cgacatggag ataaagaacg agataaataa gttaaagaaa ataatcaaca
     3721 ataaagaaag tgaatgtaac gaattaatat gtaagctaga aaaatcaatt aacgatcttt
     3781 ttgaggaaag cagaaaaaga gtaagcttct catctggaga gctagagata gaaagaattg
     3841 acaaaaaaga agaactatac ctgtcaaatc tatcttctgg tgaacggcaa gtaatttaca
     3901 ttatgcttaa agccgctaac acctccaagg aagaaactgt tttattaatg gatgaaccag
     3961 aaatatcatt acacttaact tggcaagaaa aactaataaa ctccatactt tatgttaacg
     4021 aaaaatgcca attgattatt gtaactcaca gtccggccat agtaatgaaa ggatggatgg
     4081 gtgcttttgt tgatatcaaa aacattcaag aggaaaccat aaatggctga ttttgaagat
     4141 atttttatag gagaggaata tcttgagccc tattgtgcaa tgattgggag taagggtctt
     4201 gtttttgtcg aggggttaaa agacatatcc ttctgggaag gaatgctaga taacaatgat
     4261 gacttaaaat acagcataaa ttatgcatct catgaaggca ctagaggcaa aaccgtttta
     4321 atgaaattta gccatctagc caacaaattc gcactatttg ctatagata
//
