LOCUS       NZ_CP071257             5266 bp    DNA     circular CON 20-JUN-2024
DEFINITION  Klebsiella pneumoniae strain MEI006 plasmid p5_klebicin_Col440I,
            complete sequence.
ACCESSION   NZ_CP071257
VERSION     NZ_CP071257.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN18062624
            Assembly: GCF_023612395.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Brilhante,M., Rohrbach,H., Endimiani,A., Schuller,S. and Perreten,V.
  TITLE     Promiscuous carbapenemase-containing plasmid spreads in three
            different Enterobacteriaceae species in the outdoor areas of a
            veterinary clinic
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5266)
  AUTHORS   Brilhante,M. and Perreten,V.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2021) Institute of Veterinary Bacteriology,
            University of Bern, Langgassstrasse 122, Bern, Bern 3012,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023612395.1-RS_2024_06_20
            Annotation Date                   :: 06/20/2024 04:05:13
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,676
            CDSs (total)                      :: 5,557
            Genes (coding)                    :: 5,432
            CDSs (with protein)               :: 5,432
            Genes (RNA)                       :: 119
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 54 of 125
            Pseudo Genes (incomplete)         :: 76 of 125
            Pseudo Genes (internal stop)      :: 19 of 125
            Pseudo Genes (multiple problems)  :: 21 of 125
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071257.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="MEI006"
                     /isolation_source="Outdoors environment of a veterinary
                     clinic"
                     /db_xref="taxon:573"
                     /plasmid="p5_klebicin_Col440I"
                     /geo_loc_name="Switzerland:Bern"
                     /collection_date="2019-08"
                     /genotype="ST3251"
     gene            complement(987..1388)
                     /locus_tag="J0G26_RS28220"
                     /old_locus_tag="J0G26_28160"
     CDS             complement(987..1388)
                     /locus_tag="J0G26_RS28220"
                     /old_locus_tag="J0G26_28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_108451947.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSVQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1388..1579)
                     /locus_tag="J0G26_RS28225"
                     /old_locus_tag="J0G26_28165"
     CDS             complement(1388..1579)
                     /locus_tag="J0G26_RS28225"
                     /old_locus_tag="J0G26_28165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1995..2108
                     /locus_tag="J0G26_RS28425"
     CDS             1995..2108
                     /locus_tag="J0G26_RS28425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049026061.1"
                     /translation="MHEPPVQSDCCALSGNCHLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(2316..2471)
                     /locus_tag="J0G26_RS28230"
                     /old_locus_tag="J0G26_28170"
     CDS             complement(2316..2471)
                     /locus_tag="J0G26_RS28230"
                     /old_locus_tag="J0G26_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323621.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_071561715.1"
                     /translation="MKKLLFLGVVTVSVVLAACQVNNVRDTGGGAVSPSSTVTGVTLDS
                     GTNGNP"
                     /name="colicin release lysis protein"
     gene            complement(2579..2836)
                     /locus_tag="J0G26_RS28235"
                     /old_locus_tag="J0G26_28175"
     CDS             complement(2579..2836)
                     /locus_tag="J0G26_RS28235"
                     /old_locus_tag="J0G26_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015366249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_010904244.1"
                     /translation="MANKTLADYTEQEFIEFIEKIKKADFATESEHDEAIYEFSQLTEH
                     PDGWDLIYHPQAGADNSPAGVVKTVKEWRAANGKPGFKKS"
                     /name="bacteriocin immunity protein"
     gene            complement(2838..5135)
                     /gene="kba"
                     /locus_tag="J0G26_RS28240"
                     /old_locus_tag="J0G26_28180"
     CDS             complement(2838..5135)
                     /gene="kba"
                     /locus_tag="J0G26_RS28240"
                     /old_locus_tag="J0G26_28180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease bacteriocin klebicin B"
                     /protein_id="WP_110190335.1"
                     /translation="MGGGFNYNGEGATGTGLDRDPYVRDSNGNAIGVKSGYHAESYGTS
                     SPALGPNGAIQITAGVIAVPGDKPRPDGGSGGGNTVNTGPAGQLLVMNKGQLGYWETRS
                     TGAGNNEHNTSVFVAVGPSEAEKTASAEKALKEKQQAEAAAKDFAAKTAAASATAEKER
                     QQAIAAATAAGQHQSVSDARNSLNNATSDVSRLKSAADSALQEAKAKRKAAIDAVPVAT
                     QAENKYQELQQKIKGLKLKNGEYGTDKWEIIGSNKEHDHWGYRFYPSGITKAQVDAAQS
                     DAVNKRNQATSLASQATAAEQDSLKATAAYNAAETRRQAAQAALNSAEQAAAAERKRQE
                     AEAAAAAAAEKKRQADAAAKAAEEARAAAEKARLMQERQAAADKLKSTDIQSVRGIPST
                     AAPAASPISWAVASLGGISLDSVTAGKAWTQIAEVMAKLRGIAGASLVGPVVATAVGLF
                     WSRDVGIGSDVVPGRDISGLMPGDALSLPDLATLIKAADSKTGVSMPVRGRIIVREGDY
                     LESQFVRTPVAGSVPVVRAALDKATGYWGYTLPAIQDVPGQTILVSPSDAPGVNGPLGL
                     AGPVPLPETIIHTGGQTTVPQGGTVTVSPAEDDIDFNDLILVFPPESGLKPLYVMYRSP
                     RNMPGTASGKGQNVGNNWMGGTSTGDGAPVPSQIADKLRGKAFGSFDSFRRAFWKAVAA
                     DPDLSKQFYPDDIERMKLGRAPTVRFRDSVGKRVKVELHHKVEISKGGDVYNVDNLNAL
                     TPKRHIEIHKGN"
                     /name="kba"
     Typing          1701..2108
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.917"
                     /coverage="99.0"
                     /evalue="2.74e-174"
ORIGIN
        1 catgtgttta tatacagtac ctggatacta ctcctgacat gagaactctg tcaacacgcg
       61 gagagataca attatctttt taattttcat taagttaatc atgagagaaa tgaaaaaacc
      121 cagacaggac gcggctcggc agagaaaaaa aattaacgat atgtgctctt aaaaaaggtg
      181 tgactgacgg acagagacct gtacaaaaag tcttggtgtt gagggtatcc cgccacccgg
      241 gcgctcccat ttaccataat cccttaattg tacgcaccgc taaaaccggc tggcggcgtt
      301 tctgacagga aaccggtaaa acaggcaaca aaccacccga aaacccgcca gaaacgcgca
      361 ggctctgttt ttacggcatg catgagtatg cacgcaagtg catagcgcga aaattcacgc
      421 cattcagcca aggtaagtcc tgtttccggc gttcttccca tccgaaaacg gtgaacgggg
      481 acgcaattta ttgcgtggcc gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc
      541 acgttcagga atcgtgttcg tcgacggccg gcaatcacgt tatggcaagt cgccggacga
      601 ctcaccacac gattccccgg cgactcttca ctagagtcgt acccagagtc gccgagtcta
      661 aaaaacaacc aaacggggag tcgggtctag agtcgggtcg ttattaactc attgttttta
      721 tagccctgag tcgttaccga ctctatgacg actcgccggg gactctatga cgagtcgtca
      781 tagagtcgga tctatgtaaa taaaatttaa aaaccgaaat ttcaggaaag taagctactg
      841 atattaaata caaatctgca aaattttaat tttgcgtagt gtgtgggtat ttttagtcgt
      901 gaggagcgtt cagagatctg gcgatttttg ggaaggttca ggaactcccc gggatggccg
      961 gagaggaaaa ctcaagcatg gcaggatcag gattttttcc agaatttcca ccatggtgcc
     1021 ggggattccg ttaattttgc ctggtgattt tcaagaagga gcatggcctg tttcaggctg
     1081 tcaatgtgcg aatccttgga caaaagtaag gtattcaggt gttcaatctc ctgtttcaga
     1141 tgacgaatca cctgttcact ctggtcagca gaggctgtat cacgctgtac agagacctgt
     1201 ccagaatggt gttcaccctt cggttgttca aagggaccaa agacacgtaa aagctctgaa
     1261 gtatctacca tttttgcatt gcgttcaccg ctacaggtgg acaggtcacc tgctttaatt
     1321 aacctgtgca gtgtcgttct actcttaccc gtcagtcggg cagcctcact aatagacact
     1381 ctggccatca gttaccatcc tgtaatgcat ccagtctcga ggacagtgta cggaaaagat
     1441 gctccgcatc atcgtggagt ttctcacaaa ggtcggcctc gacatcgaga tcaagctcgt
     1501 taagtttctc cagcaggagc agagactgat tctggatgaa tttagccatg ttcagcgctg
     1561 ttttctgttg tttcgtcata agaagcctca gagcgtcagt gctcagtgta agaacagtct
     1621 cttttccgct tcctcgctca ctcagtcgct acgctccggt cgttcggctg cggcgagcgg
     1681 caagaccgtt cttataaaag ctcgagaaaa ccgagcgagc aagggaggcg gaaaagagga
     1741 ggccgcccag cggccgtttt tccataggct ccgcccccct gacaagcatc acagaatttg
     1801 acgctcaaat cagtggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1861 tgatggctcc ctcgtgcgct ctcctgttcc tgccattcgg tttaccggtg tcattccgct
     1921 gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     1981 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc ttatccggta
     2041 actgtcatct tgagtccaac ccggaaaggc atgcaaaagc gccactggca gcagccactg
     2101 gtaactgatt tagaggagtt agtcttgaag ttatgtgcca gttaaggcta aactgaaaga
     2161 acgaatttgg ttactgcgct cctctactaa gccagttacc tcggttcaaa gagcgccaac
     2221 tcctcgaacc ttcgaaaaaa ccgcccgcca aggcggtttt ttcgtttaca gagcaagaga
     2281 ttacgacgat ccaaatggga tctcaagaag atcctttacg gattgccgtt cgtaccgcta
     2341 tcaagcgtga ctccggtcac cgtcgatgaa ggtgaaacag ccccacctcc ggtgtcacga
     2401 acattattga cctgacatgc cgccagaacc acagaaacgg taacaacccc caggaaaagt
     2461 aattttttca tatatcacct tttattttta ttattctagg caaatggata tctagtaatt
     2521 attattcaaa tccagagtta aaaatgtgcg gattcatcac ttgaacccgc acattcaatc
     2581 acgatttttt aaaacctggc ttaccgttag ctgctcgcca ctcttttact gtttttacaa
     2641 caccagcagg agagttatcg gctcctgctt gaggatgata aataagatcc caaccatctg
     2701 gatgctcagt caactggctg aactcataaa tagcctcatc atgctcagac tcagtagcaa
     2761 agtctgcctt tttaattttt tcgataaact caataaattc ctgctctgta tagtcagcca
     2821 aagttttatt agccatttca gttcccctta tgaatttcaa tatgacgttt aggtgttaat
     2881 gcattcaggt tatctacgtt atagacatca ccacctttag aaatttcaac tttatggtgt
     2941 agttcaacct taaccctttt acctacagaa tctcggaatc gaactgttgg agctcgccct
     3001 aatttcattc gctctatatc atcaggataa aactgcttac tgaggtccgg atcagcagca
     3061 accgctttcc agaaagcccg acggaaagaa tcaaaactac cgaaagcctt cccacgtaat
     3121 ttatctgcaa tctgggaagg aacaggagca ccatccccgg tactggtccc ccccatccag
     3181 ttatttccaa cgttctgacc tttaccactg gctgtccccg gcatgttacg agggctacgg
     3241 tacatcacat acaacggttt aagaccggac tccggcggaa ataccagaat caaatcattg
     3301 aaatcaatat cgtcttctgc cggcgaaact gtcacagtcc ccccctgagg aaccgtagtt
     3361 tgcccaccgg tatgtataat agtttcaggc aaaggaaccg gcccagcaag tcccagagga
     3421 ccattaacgc ccggcgcatc tgacggactc accagtattg tctgtccggg cacatcctgt
     3481 atcgccggca acgtatatcc ccagtaacca gtagctttat ccagagcagc ccgaacaacc
     3541 ggaacactac cggcaacagg tgttcgaacg aactgagact ccagataatc gccttcacgc
     3601 acgataatcc ggcctcgaac cggcatactg acacccgttt tactgtcagc agctttaatc
     3661 aaagtggcca gatcaggtaa tgacagtgca tcacccggca tcagcccgct gatgtcacgt
     3721 ccgggcacca catcactgcc aataccaaca tcacgtgacc aaaacagccc tacagcagtt
     3781 gccaccacgg gaccaacaag actcgcaccg gcaatacctc gtagtttagc catcacctca
     3841 gcaatctgcg tccaggcttt ccctgcagta acactatcca gcgatatacc accaagtgat
     3901 gcaacggccc aggaaatggg tgacgctgca ggcgcagccg tagacgggat cccgcgaaca
     3961 gactgaatat ctgtggattt cagcttatct gctgctgcct gacgctcctg catcagcctg
     4021 gctttttccg ctgccgcacg cgcttcctct gcagcttttg ctgctgcgtc tgcctgacgt
     4081 tttttctcag cagcagctgc tgcagccgcc tcagcttcct gccgcttacg ttcagcagca
     4141 gcagcctgtt cagcagaatt taacgccgcc tgagcagcct gacggcgcgt ttctgccgca
     4201 ttataggcag ctgtagcttt caggctgtcc tgttctgctg ctgttgcctg actggcaaga
     4261 ctggtagcct gatttcgctt attcacagca tcgctctgcg ccgcatcaac ctgagctttg
     4321 gtaattccgg atggataaaa cctgtatccc cagtgatcat gctccttatt agagccaatt
     4381 atttcccatt tatccgtacc atactcacca tttttcagct tcaggccttt aatcttctgc
     4441 tgcagctcct gatatttatt ttccgcctgg gttgcaacag gtacagcatc aatagcagcc
     4501 ttccgttttg cctttgcctc ctgcagtgca ctgtctgcag ctgatttcag acgagacaca
     4561 tctgacgtcg cgttattcag gctattacgg gcatcagaaa ctgactgatg ctgacccgct
     4621 gcagttgcag cagcaattgc ctgctggcgt tctttttccg ccgtggctga tgcggcagca
     4681 gttttagccg caaagtcttt tgctgctgct tctgcctgct gtttttcctt taacgccttc
     4741 tctgcagaag cagttttttc tgcctcagaa ggacctacag caacaaatac actcgtatta
     4801 tgctcattgt tacccgcgcc cgtagaacga gtttcccagt atccaagctg acctttattc
     4861 atcaccagga gctgtcctgc aggtccggtg ttaacagtat tcccaccacc actaccaccg
     4921 tcaggtcggg gcttatctcc aggcacagca ataaccccag cagtaatctg aatagcgcca
     4981 ttaggcccta aggctggact tgacgtccca taggactccg cgtgatagcc tgatttaaca
     5041 ccaatagcat taccattgct gtcgcgaaca tatggatcac gatccaatcc ggtgccagta
     5101 gcaccttccc cgttatagtt aaatccacca cccataactc tctcctttgt tgttattttt
     5161 atcgttttcc tgcttgtcag aatctggtgt gcggtttatc gccttcgaac ctgtatccgg
     5221 aggctgttat tagttctctg ccacaccaaa gtactgtata taaaca
//
