LOCUS       NZ_CP071089             4052 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 3347689I plasmid p3347689I_3, complete
            sequence.
ACCESSION   NZ_CP071089
VERSION     NZ_CP071089.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17736022
            Assembly: GCF_018128465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Polycolonization with MDR bacteria in a patient transferred from
            intensive care unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 129x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 11:00:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,873
            CDSs (total)                      :: 5,750
            Genes (coding)                    :: 5,622
            CDSs (with protein)               :: 5,622
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 46 of 128
            Pseudo Genes (incomplete)         :: 76 of 128
            Pseudo Genes (internal stop)      :: 21 of 128
            Pseudo Genes (multiple problems)  :: 14 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071089.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="3347689I"
                     /isolation_source="outer malleolus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3347689I_3"
                     /geo_loc_name="Switzerland"
                     /collection_date="2020"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(137..349)
                     /locus_tag="JY394_RS29205"
                     /old_locus_tag="JY394_29205"
     CDS             complement(137..349)
                     /locus_tag="JY394_RS29205"
                     /old_locus_tag="JY394_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(353..838)
                     /locus_tag="JY394_RS29210"
                     /old_locus_tag="JY394_29210"
     CDS             complement(353..838)
                     /locus_tag="JY394_RS29210"
                     /old_locus_tag="JY394_29210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_055314654.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIAMSGSILWWQGQQIT
                     GNYQTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEEGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<876..1553)
                     /locus_tag="JY394_RS29595"
                     /old_locus_tag="JY394_29215"
                     /pseudo=""
     CDS             complement(<876..1553)
                     /locus_tag="JY394_RS29595"
                     /old_locus_tag="JY394_29215"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1516..1857)
                     /locus_tag="JY394_RS29220"
                     /old_locus_tag="JY394_29220"
     CDS             complement(1516..1857)
                     /locus_tag="JY394_RS29220"
                     /old_locus_tag="JY394_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3223..3681
                     /locus_tag="JY394_RS29225"
                     /old_locus_tag="JY394_29225"
     CDS             3223..3681
                     /locus_tag="JY394_RS29225"
                     /old_locus_tag="JY394_29225"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     Typing          complement(1945..2124)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          2101..2587
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="93.0"
                     /evalue="5.96e-162"
     Typing          complement(22..1526)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtcactggtg aagcctgtca gcccagcgtc ggaccgttat agctccgctg acgggaacgg
       61 gcttccgcca gctctttttc gtgctgtcgc tgctcgtatg cccgttcggc ggccaccgcc
      121 atttcatgcc gctgcactac tggctcaaac gctgtaactg cctgctcaag ttcgccacct
      181 gctgactcaa gcgcgtgacg cgctcgttca gcgccgcgtt ctcccgttgc ataagaccag
      241 acatcttctg ccattccgcg aaggcgctct cccactcgtc cagcctttgc gagtagtcct
      301 gatgtagctg ctctaatgcg gtcagcaact gtttttccag ttccgtcatg ggctatttcc
      361 tctccagtac catccagttc ccttcctccc cgtaagctcc cgctttcggg ttcactttga
      421 cgcaggacag tttcttctcc ccgcagagcg acagctgaac gccgtggttt ttctccgaca
      481 acatggcctg cgtccgctcc tgcgcccgga tagtctggta attcccggtg atctgctgcc
      541 cctgccacca cagaatgctc ccgctcatgg cgatcagcag taccgagacc agggtgatgg
      601 tcagccacgt ccggccgacc atacgcagca cgctcaggcg gttcgactgc atcgccgcgg
      661 tcataccctg ctcgtgggcg ctgatggcgt cgctgattct cttcgcgctt gcgctcagtt
      721 ccgcttttac agatttctcg tgctcgctga atgcggcttt cagcatctcg ccggtactct
      781 gctgctgcgc tttcgattgc tgctccaagt ccttcgccag cgctaaaaga ctgctcataa
      841 atggctccct tcagtcggat gtttcgcccc ccgtccgggt cggcaatgct gatactgttt
      901 tttgtagtgc ggaccacctc aaaacctgcc gctgtgagcg cacctgtgac gtcctcgcga
      961 gttttgagct ccccggatga ggctaaggcc agcaacccac gtgtaatcgc ctgagcggct
     1021 tccagcttcg tttccggcag tgcggacggc gtaaccagta accgccggtt ctccggggcg
     1081 tttggatcat gcagtcccag tctgccgttc accacggtct gccaggcatc gatgcgcggc
     1141 cggtcggcgc gatcgtaata cggctgcagg cgtttcccgg tcagcagttc agtgttgggg
     1201 atcaggaaat tcagctccag ccgccccttg tcggtgtgct ccacccacag aatgctgtac
     1261 tggtctttat cgagcccggg catcagaacc cgctcaaagc tggccatgat ctgctcccgc
     1321 tgcccgggca gcagttcagc ctccgcgaac gacagcacgc cggaggtgta ctttttcgca
     1381 tacggcgagg catcgataag ctcccggact tcctccggtt ttccctgcag cacggtcgcc
     1441 ccctcgcgct gccggtcttt tcccagcagg taatccaccg gaccggcacc accaccgcgc
     1501 ccccggggat gaaatttaac gatcatcatc cccccctttt tccagcaccg catgccgcag
     1561 ccgctccagt cccgcgtcta ttgccatcag ggcggccaca atctgcaccc gatcatgacc
     1621 ggcaccaacg gcattcactt tccgggcgat ctggttcagg ttgttaccca taccggcaag
     1681 ctggcgcagc agcgccggcg atattgacgg cagtctgcag gcacgcgccg gcttctcatc
     1741 gaggcaggac tgacgcatcc acgccgccag ctgcctgccg tcgcaccgct cgagcagccg
     1801 ccggtgttcg tcttccgtca cccacatcgt gagcatcttg ttgcgtttgt ctgccagagt
     1861 agcctcctga taacaccggg acgggcggga aatgtttccc ggtcggcacg gtgttggaca
     1921 agccgcaggc gcgtcagtgg cttttagcgg gtttcggggc gcagccctga accagtcacg
     1981 tagcgctagc ggagtgtata ctggctaaat atgttacggc ggagtggtaa tgtatgaaag
     2041 tgcttcatgt gggaagggga aaatgtggct gacgggcgta agtgcaacat gctggtaaag
     2101 gtgaaatgac gcctcctcgc tcactcagtc gctgcgctcc tgccgtgaga ctgcggcggg
     2161 cgttaccagc tcacagatga cgggatacgc aagcagcgct caaatcagga gaagccggga
     2221 agaggatgcg gcgttgccgt ttttccatag gctccgcccc cctgacgagt atcataaaat
     2281 ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc
     2341 ccctggtagc tccctcgtgc gctctcctgt tcctgccatt cggtttaccg gtgtcattcc
     2401 gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc
     2461 gctccaagct ggactgtatg cacgatcccc ccgttcagtc cgactaccac gctcttttcg
     2521 gtaactatcg tcttgagtcc aacccgaaaa ggcacgacaa atcgccattg gcagtagcca
     2581 ctggtaaaca ggatgtgcga gagatgtatt cagagttctt gaagtggtgg ccttactgcg
     2641 gctacactgg aaggacagct ttggtgacat gtctcgcaca agactgttac cacggttagt
     2701 aaatctgcca ggattaacta accttcgaaa aaccacctgc cagggtggtt ttttcgttta
     2761 cagagcaaaa gactacgctc agaaagagat tatctcaaga agatcatctc tttctcttac
     2821 tcaagtcaaa gcaaaaaaac accactccat cacaaactca ggttatagtc ggtatgcaac
     2881 taataacact gtaatttctg tgcaaaatcg aggttatgca tatgcaaatc aacatcaact
     2941 gcaagagtat cccaacatct ttcgatgttg tctgctcgaa ttgtgaatgg accggcgtgg
     3001 ctacattaga ttatccggaa aacgtaaaaa ccctatctgt cggaggagaa ataaatttca
     3061 ctcataatga cgagacgcca aaatgtccac aatgtagcgt tggatatata tatggtaaaa
     3121 gtggcactta taaacgtaat agtgcaacta accatatgga cagaatcgga gattataaag
     3181 agtaaataat acgcatcaca gcaatctaac gagggaaata taatgattga aaccacacta
     3241 cttgccgttc tcactggcgc attaagcaat cttacttctg atgcaatcaa aggcatcata
     3301 gggaagttag caagtaaaca tcctgaagtt gaacaacaag cctcactgct tgaaacacca
     3361 ttaccttttg acgaagttga taaattattt tctcagatgc agggggccat tcaggctgca
     3421 gcagccaatg gtgaaattca gctagatgga gctgaattaa aagcattgag cggaataaaa
     3481 tttgatcacc aagccggaac tgttttgata aatggatctc aagtacaagc gtcaatttta
     3541 gtcactggtg gaggtcaaca tgctactgga aaaacgatca tacaaggtga tacgacaatg
     3601 actagccagg gtacaagtat aaaaatgtca ggtggtgctc aaatagtaat gactggtgga
     3661 gcccgaatca ttcagaacta atatgattta ccataacatc tcttgcccgc accgctccgg
     3721 acatgttcgt cacaaacggg gcggcagtca ggtaaaaccg gcaacaaacc acccgataca
     3781 ccgccccgtt cgtgacagat aaattttacc cggctgcatg gtcatgcagc cagatgcata
     3841 cggtctgatt tcaggccaga atctggatga acgccttaaa aacaggaaaa tgcttctcca
     3901 gccagtccca gatctccagc gccatgtaca ggcagttcag caccgtctca atcacgttaa
     3961 acagtctcat aatccctccg ttgtatacgc cgtgatgttt tcatcacgac cgtatttagc
     4021 gggaaattta tgagcccttc ttcgtggggt tt
//
