LOCUS       NZ_CP071088             9730 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 3347689I plasmid p3347689I_2, complete
            sequence.
ACCESSION   NZ_CP071088
VERSION     NZ_CP071088.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17736022
            Assembly: GCF_018128465.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Polycolonization with MDR bacteria in a patient transferred from
            intensive care unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 129x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 11:00:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,873
            CDSs (total)                      :: 5,750
            Genes (coding)                    :: 5,622
            CDSs (with protein)               :: 5,622
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 128
            CDSs (without protein)            :: 128
            Pseudo Genes (ambiguous residues) :: 0 of 128
            Pseudo Genes (frameshifted)       :: 46 of 128
            Pseudo Genes (incomplete)         :: 76 of 128
            Pseudo Genes (internal stop)      :: 21 of 128
            Pseudo Genes (multiple problems)  :: 14 of 128
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071088.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="3347689I"
                     /isolation_source="outer malleolus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3347689I_2"
                     /geo_loc_name="Switzerland"
                     /collection_date="2020"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            join(8467..9730,1..698)
                     /locus_tag="JY394_RS29160"
                     /old_locus_tag="JY394_29160"
     CDS             join(8467..9730,1..698)
                     /locus_tag="JY394_RS29160"
                     /old_locus_tag="JY394_29160"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            698..1429
                     /gene="mobC"
                     /locus_tag="JY394_RS29165"
                     /old_locus_tag="JY394_29165"
     CDS             698..1429
                     /gene="mobC"
                     /locus_tag="JY394_RS29165"
                     /old_locus_tag="JY394_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            1436..1966
                     /locus_tag="JY394_RS29170"
                     /old_locus_tag="JY394_29170"
     CDS             1436..1966
                     /locus_tag="JY394_RS29170"
                     /old_locus_tag="JY394_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1994..2173)
                     /locus_tag="JY394_RS29175"
                     /old_locus_tag="JY394_29175"
     CDS             complement(1994..2173)
                     /locus_tag="JY394_RS29175"
                     /old_locus_tag="JY394_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2242..2619
                     /locus_tag="JY394_RS29180"
                     /old_locus_tag="JY394_29180"
     CDS             2242..2619
                     /locus_tag="JY394_RS29180"
                     /old_locus_tag="JY394_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(2681..2980)
                     /locus_tag="JY394_RS29185"
                     /old_locus_tag="JY394_29185"
     CDS             complement(2681..2980)
                     /locus_tag="JY394_RS29185"
                     /old_locus_tag="JY394_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2970..3233)
                     /locus_tag="JY394_RS29190"
                     /old_locus_tag="JY394_29190"
     CDS             complement(2970..3233)
                     /locus_tag="JY394_RS29190"
                     /old_locus_tag="JY394_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            3610..4695
                     /locus_tag="JY394_RS29195"
                     /old_locus_tag="JY394_29195"
     CDS             3610..4695
                     /locus_tag="JY394_RS29195"
                     /old_locus_tag="JY394_29195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            4692..6881
                     /locus_tag="JY394_RS29200"
                     /old_locus_tag="JY394_29200"
     CDS             4692..6881
                     /locus_tag="JY394_RS29200"
                     /old_locus_tag="JY394_29200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     Typing          complement(7087..7667)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          699..1426
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 tcaacggcgg cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg
       61 tgcagaagat gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga
      121 ggtggccgca cgcgagcatc atgctcgatg agattaaata cctgctgtcg aagtacgtgc
      181 tgaacgcgct ggggacgctg cgcagtcggg actgcaacct gctgctcgcg caccagtcgc
      241 tgggcgactt cgggcagtgc ggccaggatc tgcccgctga gtttgtgaaa accacggtgc
      301 tggataacac gccgatccgc tggttctacc gggcggccag ccaggagtcc gcgcagtggg
      361 cggcgggcca gaccggggaa atccgggtgg acgtggagcg ccgccgggcc agccgggagg
      421 cggggaacgt ggagcatatc agcggggaca gcttcattca gaaggaggcg cggccgctgt
      481 ttgacgtgaa caccctgcag cacctgcctg acgggtttgc ggtgatgacc gggctgggcg
      541 tggcccggat ggcgttcagc agcccgctgc gggttgaacg ccgggaaatc ccgctgcagc
      601 gcttcccggt gctggcgaaa acggacccgc tggcggagta ccagccggag gccgggcggc
      661 agcggccggg tgatgatgat tttgcggggc tgtactgatg gcactggagc gatacaacgt
      721 cagccacgcg aagcggcagg cgcggaacgc ggagaaaacc cggctgaccc tgcgctggct
      781 gcgggaagag ctgtgttcca cggcggagct ggttgcgcga cggctgggga ttgcggcggt
      841 gcagccggtt taccgcttcc tggacagcct ggtggcgaaa gggctgctgg tccgggcgaa
      901 atacccggtt gacggccgtc aggtcagcgt gtggggactg acgccgcacg gggtggcgtt
      961 ctcgttcgac gaggacgaac cgctgacgga cgttattccg tttcagccgt caagggtgtc
     1021 ggcggcgcag ctgccgcacc ggctggccgt gcagtccctg cgtctcgcca tggaggcccg
     1081 gggggcctcc ggctggcggt acctgcaccg tatggccctg aaggggatga aggtgccgga
     1141 cgcgctggcg gagctggacg gcagaacggt ggcgtttgag gtggagcgca ccgtgaagag
     1201 ccgcaggcgc tatcaggagg tggtgtccgg gtatctgttc aatcgcaaag cgaacggcat
     1261 cgatgaaatc tggtacatct gcccggaccg ggcgacgcag gtcagggtgc agcgggcgat
     1321 cctgtcggtg gatgaaatcg tgaatccaca gaccggcgag gcccgcaaaa cggcggagct
     1381 ggaccgggag cggctgtttg cgtgctttaa atttatgaca acggagtgag ggattatggc
     1441 gctgtacatg gtatttattg aagacgcgga ggaccgggac ggcgagcgct gcatcatgcc
     1501 gaccagcgag catcttgaag atatggtggt gctcgcctgg cagaccgggg cgtatgtttt
     1561 ctgttttgtc gaccgggccc agcatctggc gatgatgaaa tccatcggga tgtatgacga
     1621 gcggctcaac agcgacgaaa aagccacgta ctttttcacc ggtaaaaatc cgcaatggga
     1681 acgggacaaa cccgcaggaa tgaagctgtg tgattacctc tatcgcaagg tggagcggtt
     1741 cgccatcgag aacggtgtgc aggtggttga tgccgggaag tgctcaccgg agcagattag
     1801 cgcgatgatt gaggtggtca gcgagcggca cggggaaagc gcggtgaacg agaagctgaa
     1861 agcggagctg atgcgggaat ataacagccg ctattttaac gtgccggtgc ctgaactgcc
     1921 ggaggacaaa cccgcccgcc ggtggtggcc gttcggcaaa aaataaacgg cagcaccggc
     1981 catgccggtg ctgttattcc ttcccgagct tatcccgcat cacctgccag agtgtttccg
     2041 catcctcgtg cagccgctcg cacatgtcgg ccggggtatc catatccagc tcgttcagtt
     2101 tctcgagtaa cagcagcgac tggtccttaa taaatttagc catattgagg gcagtttttt
     2161 cctgcttagt catgttttct gtccttccgg gtgtggtgtg cttcctgtcg ggtatactgt
     2221 ctcaaaaccg gaggtgagac aatggcgtgg gtgacggtga gacaggcgac ggaactgacg
     2281 gggaaagccc ggagttccct gtatagggat atggcgaaag gccgcgtatc ataccgtact
     2341 gaagctgatg gtggtcgggt ggtggatacc agcgaactta tccgggtgta cggtgaatta
     2401 agacagattg agactcacga gcgggacggg ttgagactgt cggatgagac tgaaaaaatg
     2461 gcctccgacg cgttgatcgc ggagataaaa gcactcaggg aggaggtggc cggactgcgg
     2521 caggaaatgc aggccatgag actgctggaa cacaaaccag agacagcaga gacaccggcc
     2581 ggaacatcgc gccgatggtg gcagtgggga aaacgctgac gcgctgtgct tgtccaaccg
     2641 gcgacggcca cgcaatgaat tgcgccccca tcaccggtta tcagcgaacg gtatggggat
     2701 tctggcgctg gccaaccacg gccagaaccc ggactttcct ggtctggtca tcaacatcat
     2761 agaggacatg ctgtcccatc agactgatcc ggcgaacgtc ctcgccatag cgtgagtcca
     2821 cttcatatag gcgcgggaag tcggccagtt tgtccgctgc agcgagaact cgatcggtaa
     2881 cgttctgcgc atcccacagg gatgaatttt ccgcaatgta gtcaaacaat gctgtcacat
     2941 cctcgatggc tccctgcttc cattccacct catacggcat cgcgtagttc ctgatagcgg
     3001 ccatgcagtc tctggcggag ttgttccgtg gtcatgcccg ggcgcgtgtc agcgcgggcg
     3061 gcggtcactt tagcgcgaac ctgatcttca tattctgcct gggccagttc ttcgttagcc
     3121 aggcgctgca acagtgcagc tttgtccggg ccaccttcag gcagtatgcg gggcagtctc
     3181 tcggccagtt cccgcaggaa attggcgttt tcatagtgtg tactgttgct cattgtggag
     3241 cctcccgggc tgatacgtcg atatgaagga gtttagcagg gggcggtctg aaagaatatg
     3301 ctcgtgtcag tcaggctgat gaagacgaaa gctggtaaaa aagggcttac gacacccatt
     3361 ttacgtcaaa ctgcacgaca tgcagttgcg tgcatagcca tgcagcgggt taaaatttat
     3421 caggcgcgtt tccggcggtt ttccgggtgg tttgttgccg gttttatccc gtagccgccg
     3481 gaaatgccct cagaccgtct gagcggtgcg cgtaatgcga cgttatggta aatcatgtga
     3541 ttaatggtag tgataatcat tatcactttt gctatattct tgtttctttt attgtaagga
     3601 aatgtaagta tggcaatcac gattaaatcc gccgttaatg tccgtagcag agtattaata
     3661 tactctttac tactgtttac tggttcagcg tatgcggctg atatagctga atcaacattc
     3721 ctgactcgtg atatcggcaa tggtctgtat gaaatgtctt ttgatggcaa aaataatagt
     3781 ctctgggcag catcatcgtc ttcattcgac agagataagg gccacggaat tgtttatcag
     3841 ttagaaccat ccactctgaa aacaaaatct gccgttaaaa tcgcacgtct tgggtttgcc
     3901 acagcactgg atgaggaaca tgagcgactt tatatcggca atactctgga cggctcagtg
     3961 acggtcattg atactctaac cggaaaagaa ctttcagtaa ttccgttaac tgagggacag
     4021 gatgaggctc atttcagacc gaccagagaa atggtcattg acaaaaaaaa tcagcgtctt
     4081 tatgtctcca gcgccgcaga taaaggggct ctgtgggtta ttgacatggc tgtcgggaag
     4141 aaaattaaaa ccattgatgg actggggcac gctgttactg gaattgcgtt aaataaaaat
     4201 ggggataata tttatctggt taacggggat ggtgagatta ttaacctgga tgccagtagc
     4261 tatgaaataa aaaaacggtt tgttgtagag cctgagaaaa aacacttttt ccttaatgta
     4321 agtctggatg aaaaaaacgg acgtgcattt attaccgacc cggatattcc cggggtgctg
     4381 gttgttgatg taaactcggg aaaaatacta cacagaattg atgttataaa ttcactggcg
     4441 attttattta atcctttacg gaacgaaatc tatatcacgc accgtaatgc aaaacaggtc
     4501 agcattgtgg acagtaattc ttattacgta aaaacaacga taaaaacccg cctgatgcca
     4561 aacagtctgt ctttatcaca tgacggtgaa aatctgtacg ttagtgtaaa acaagaaaaa
     4621 aaagagatga gtactatgaa ggattacata ctcaaaatcg aattatctaa aattgctatg
     4681 gagccagaag aatgaataaa tatttagggc tgagcatagt atatatgatg tatacatcat
     4741 ctgttatggc tgatgattct ggcgcagtta gtaaagataa aaatatttca ccagaagaaa
     4801 cattaattgt tcatgggacc gctgtttctg aaagcacaac agaaggaaca gattcataca
     4861 caacccagtc agccaataca gcatcaaaga tgaatctgtc attccgggat acacctcagt
     4921 caactacagt cattacgcga cgaagaatgg atgatgtagc agcaaaaaat attattgatg
     4981 tggttaaaac cgctcctgga ttatatgtca gtacagcaga aggttccgga agaccgaatt
     5041 ttatgtccag aggatttaat gctaatgtca tgtatgaagg atttagcctg ccgtggaatt
     5101 catacattcc aagttcacaa actagttcag ctatttttga ccgggtggag atagtccgcg
     5161 gggcaacagg tcttgttcag ggtgccggag acccttcagc cgcgataaat atgatacaca
     5221 agcgtccgac ccggcaatat caggccagca ctaacatcgg tgccggacgg tggaatgact
     5281 accggggtgg ggttgatgta tcaggaccac tgaatgacag cgcgacctta cgcggaaggg
     5341 cagttgtgga tggcgaaaac agtgagacct tcagggatag tgagaaacgc aggaataacg
     5401 tcatttacgg aattatggag gcagatctga ctgataatac cctacttacg attgggggat
     5461 atcagcagta tgaatataac aatcattggt ggtatgactt acctatttct ggtaccggac
     5521 accaccttaa tcttcctcgc tcgacgttta caggcaatga ctgggagttt gaaagaagta
     5581 aaagtaaaac tgcatttgca actctggagc agaagttcag taatagctgg aagctgaaat
     5641 tttcaacgct gcaaaactgg agaaatgctg acctgttagg gacggcaaca taccggcata
     5701 aagatacccg acaaagtatg tatcagcaag tatggggatc actgagggaa tataattcca
     5761 aaagctatga cctgtcgctt aatggggact atgctttatt tggtcgtgtt catcaattcg
     5821 tggctggaac aactcagcaa aaatacaccg ataaagatac aggttataca tattccccaa
     5881 tatatattga gaatgttaat atatttgatc atggttcaca ttcttcgaaa aaacccatga
     5941 agatgtcagg tgatgcatcc tacgacgtca ctgaacaaaa aagcgcctac gcagcaactc
     6001 attttctact tagtgataac tggaaaatat tgctgggtgg ccgtcttgac tggtatgaat
     6061 atacaagtaa aggtacatcc attaatacag gttataaagt taccagaaat atcaccaaat
     6121 atgcaggttc aacatacaat cttgacgatc atcataccat ttatgggagc tataccgata
     6181 tattcaatcc acagtcaagc accgacctca atggtaaagt gatcaagcct attgttggta
     6241 agaattatga gacaggtatt aagggtgagt attttaacgg cttgcttaat gcctctctgg
     6301 cggtatttca ggtagaccag gaaaatcgag ccaggctgct tcccgatcag acagggtgtg
     6361 ttactaatgg caatcctcaa tgctatgagg ctagcggtaa agtacgcaca aaagggatag
     6421 atttcgaact tcagggcatg gttacgccac tctgggaaat tggggtggga tatacatatg
     6481 cagatacgcg atatataaaa gatggagatc gtagtaatga aggcaagcga ttcaatactg
     6541 ccgtgtcgcc acaaaattta tttaagattt cgagcatgta cacctttgac ggaaaattaa
     6601 caaactggcg agcaggagta aacacgtact ggcaaagtaa aatatatgct aacataacag
     6661 acacaaggga tggcaatccg gttattaata aacaaaaggc ttattcaaca actgacatta
     6721 ttattggcta tactccaatg ccacaactgg atttacagct caatataact aatgtctttg
     6781 ataaggttta ttatacatcc ataggtgaat cgattggata tggcacggaa acatatggag
     6841 aaccaagaaa tgttaaaatt agtgcaacct atagattcta accatgcgga ttgggtcttc
     6901 tcctcggatt tgcccctata aatccgtctt ttgtggttcc ggcgtacaac cccctgtttg
     6961 ccacttcagt tgatggttaa tcaaatcatg tagaggatct tcttgagatc ctccatggtt
     7021 cgtcgtaatc ttttgccctg taaacgaaaa aaccacctgg tgaggtggtt tgatcgaagg
     7081 ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca
     7141 aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta
     7201 gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg gcgtttttgc
     7261 gtgtctttcc gggttggact caagtgaaca gttaccggat aaggcgcagc agtcgggctg
     7321 aacggggagt tcttgcttac agcccagctt ggagcgaacg acctacaccg agccgagata
     7381 ccagtgtgtg agctatgaga aagcgccaca cttcccgtaa gggagaaagg cggaacaggt
     7441 atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgaccc gccggaaacg
     7501 gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg gttgagcctc
     7561 acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaattctggc aggagggcga
     7621 agcctatgga aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg ttagtcccct
     7681 gcttatccac ggaatctgtg ggtaactttg cgagagttag cagcgcccgc cgcagtctca
     7741 cggccggagc gtagcgactg agtgagggag gaggcgcaat atcccccctc ccgcagacgg
     7801 ccaccggacg cgcggatgtg acatattgca ccgtgttacc gcctttccct tccctgccga
     7861 taaatcattc agataggtga agtctgtcgg ttttctgctg aatattttac gttaacctat
     7921 ctctgttgct tttttctcct tgcggagaaa tgcaggtcat ccccgctggg gcgtggctga
     7981 cttcaggagg aaatcccctg cttacgctgg ctggacgcca ccgacgcacg gtctttcccc
     8041 ctctttctgg ctcagctcgg ctggacgccg gccacgccag aaattcaccc ggcccccgtc
     8101 aggctcacgc ctgaccaccc acaaaaaccc acggtgctga acgcacctga cgaagcagca
     8161 aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct gacgggggcc
     8221 ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc tggggcgtgc
     8281 tcgttgttcc tccggggctg cgccttttcc gcttggggcg gaaagaggct ggcctccgtg
     8341 gtgaaagctg aatgcaatca ccacggacgt gaccgccgcc gccatgcctc ggggcatgtc
     8401 cgcggcttgt gcagcagggg ctgcacacca cgccggtaaa cccgtcgtct gaaggagaga
     8461 gattgcatgt ttaatacgga ttcgcttgcc tggcagtggc atatcccgga gctggcccgg
     8521 cagatttacc cgtgggtcag ctggctgtac agggagtatt acggtctggc cggggctttc
     8581 ctggttatcg gcctgttttt cggtctggct gaactctggg ccggacggat gcgttttgtg
     8641 gggctgagtc ataaggagcg acgggagtat gccgatcgct ataagcgcga taataaccgg
     8701 actgcgccat tttcggtgct ggcttcgctg gattcgcccg ggcggatggt cagggcacgg
     8761 cggcatgtct ggaactacgc gtactggctg accggcgctg ccggtctggc ccttctgggg
     8821 gggctggttg tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg tccggacctc
     8881 tacgcgaacg cagattatta cctgctgcag ccggctaacc tgttcgggat tgtctggtgt
     8941 gccggagggc ttgtcgccgg catccttctg gcgcggctgc tggcatttct gctgcttgac
     9001 ggtcattttg cggccgctga cgaggcggtg aatgcgaaac tgaaccagga gagccggcgc
     9061 agcagccagc ggaccggaga aatgaccgac gtccgccacc tgcatttcgg ggagccggta
     9121 ccggttaacg cgctggccga tttttccaca gagcaggccc ggaaacagca ggcggtgttt
     9181 ctgggtaagg atgagcaggg ccagccggtg ctggtcccgc gtgatacctg gcgcaagacc
     9241 aatattcaga tcctcggcct gcccgggagc ggcaagagcg tgatgggcac caacgcgctc
     9301 attcgctgcg tacgcgattt cggggacgcg gtggtgtact tcgaccccaa aggggacgcg
     9361 tgggcgccgc acgtcttccg ggcgcactgc ccggacttca ccctgctgga tctgcggccg
     9421 gggaaaccgg cgcagctgaa tctgtttcgc gatctcgacc agtacgccct gaagaatctg
     9481 ctggttgccg ggtttaacct gagtgagacc ggcgacgtgg cggaccacta ccgcatcagc
     9541 gagcagaaag cggcgaagct gattgcggag cagttcccgc agggggcaaa cattcagcag
     9601 gtgctggcgg cggcatacgc gctgccggag gccctgaaaa aggacgtgaa ggggctgatc
     9661 accaagctgg agaacgtggc cgacctgagc gtcctgcaga cggacagcgg catcgacgtg
     9721 gcggggataa
//
