LOCUS       NZ_CP071085             4251 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 3347684I plasmid p3347684I_5, complete
            sequence.
ACCESSION   NZ_CP071085
VERSION     NZ_CP071085.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17736023
            Assembly: GCF_018128445.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Polycolonization with MDR bacteria in a patient transferred from
            intensive care unit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Moser,A.I., Campos-Madueno,E.I. and Endimiani,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 255x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 10:18:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,573
            CDSs (total)                      :: 5,449
            Genes (coding)                    :: 5,331
            CDSs (with protein)               :: 5,331
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 49 of 118
            Pseudo Genes (incomplete)         :: 65 of 118
            Pseudo Genes (internal stop)      :: 20 of 118
            Pseudo Genes (multiple problems)  :: 14 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP071085.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="3347684I"
                     /isolation_source="central nervous catheter"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3347684I_5"
                     /geo_loc_name="Switzerland"
                     /collection_date="2020"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(join(4089..4251,1..314))
                     /locus_tag="JY393_RS27680"
                     /old_locus_tag="JY393_27675"
     CDS             complement(join(4089..4251,1..314))
                     /locus_tag="JY393_RS27680"
                     /old_locus_tag="JY393_27675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226564495.1"
                     /translation="MSFTVASAENITHTKPLARSDLYLTCKGYQVKGASVINVEKPSYL
                     EGTGEQVEFAIISLGDASDGLKVFFDSWGFSPFGLIESHVMHEHWSLVHVGGRVSVKGE
                     KIDYSFVDNQPQEGGSYKPKTGVFLYGCINSDKRPSWVEKIYTRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(485..700)
                     /locus_tag="JY393_RS27685"
                     /old_locus_tag="JY393_27680"
     CDS             complement(485..700)
                     /locus_tag="JY393_RS27685"
                     /old_locus_tag="JY393_27680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_058671277.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(704..1189)
                     /locus_tag="JY393_RS27690"
                     /old_locus_tag="JY393_27685"
     CDS             complement(704..1189)
                     /locus_tag="JY393_RS27690"
                     /old_locus_tag="JY393_27685"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_211652428.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESE
                     KRISDAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLTASGASILWWQGQQIL
                     DNYTTIREQKRTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1224..1928)
                     /locus_tag="JY393_RS28095"
                     /old_locus_tag="JY393_27690"
                     /pseudo=""
     CDS             complement(<1224..1928)
                     /locus_tag="JY393_RS28095"
                     /old_locus_tag="JY393_27690"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1867..2190)
                     /locus_tag="JY393_RS27700"
                     /old_locus_tag="JY393_27695"
     CDS             complement(1867..2190)
                     /locus_tag="JY393_RS27700"
                     /old_locus_tag="JY393_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_170874913.1"
                     /translation="MLTMWVTEDEHRRLLDRCDGKQLAAWMRQTCLDEKPARAGKLPSL
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3237..3392
                     /locus_tag="JY393_RS27705"
                     /old_locus_tag="JY393_27700"
     CDS             3237..3392
                     /locus_tag="JY393_RS27705"
                     /old_locus_tag="JY393_27700"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_167493803.1"
                     /translation="MNSKQKCSHSNLEKEYYLGSATGDYKCLDCGEVGFGKDWPEKERS
                     KNDNEA"
                     /name="hypothetical protein"
     gene            complement(3420..3833)
                     /locus_tag="JY393_RS27710"
                     /old_locus_tag="JY393_27705"
     CDS             complement(3420..3833)
                     /locus_tag="JY393_RS27710"
                     /old_locus_tag="JY393_27705"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023206708.1"
                     /translation="MKIIKWASLSGVLLISACGVSQNEVNSANYGSKPIDNDAKVIGMI
                     KESLKDPDSLKVISITTPARGFSKYGFGSTEHGWFTTITYNAKNSYGGYVGAKSSTFVY
                     LNGDYKVAVPMSNGERVMLDKVSFSCKDNCQTK"
                     /name="hypothetical protein"
     Typing          2457..2946
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.501"
                     /coverage="81.0"
                     /evalue="0.0"
     Typing          complement(394..1877)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.515"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttttcaccct tgactgaaac acgcccccca acatgaacta aagaccagtg ctcatgcatt
       61 acatgacttt caatcaatcc aaaaggagaa aaaccccatg aatcaaaaaa aaccttgagt
      121 ccatcagatg catcaccgag agaaatgatt gcaaactcaa cctgctcacc tgtaccttca
      181 aggtaagaag gtttctcgac gttaattaca gacgccccct tcacctgata acctttacag
      241 gtaagatata agtcgctcct ggctaacggt tttgtgtgtg taatgttctc cgcagaagcg
      301 actgtaaatg acataaccaa aactactgag actgagcaag cccagaaaca cttcataacg
      361 catccctttt cattgattaa ctacattcca agctcaaaac ctttctgccg ctgcgatcgt
      421 tccagctcca gcgttttctg atgctgcgcc gcccgctcct gctgcctgtg tgcttcaatc
      481 gagttcagcc tttcgacaac cggcgcagct gatcgctcaa actctgcacc tgcctgctca
      541 agcccgtgac gcgctcgctc agcgccgcgt tctcccgttg cataagacca gacatcgtcc
      601 gccattccgc gaaggcgttc tcccactcgt ccagcctttt cgagtagtcc tgctgtagct
      661 gctctaatgc gctcagcaac tgtttttcca gctccgtcat gtgctatttc cccgccagta
      721 tcatccagct cgaatcctct ccgaaccgtc ccgcttccgg gttcaccctc acgcagcggc
      781 gtctctgctc tccgcaggtc gagagctgca cgccgctgtt cctctcggac agcatggcct
      841 gcgtgcgctt ctgctcccgg atggtcgtgt aattgtcgag tatctgctgc ccctgccacc
      901 acagaatgct tgcgcccgat gctgtcagca gtgcagacac caggacgatg gtcagccacg
      961 tctggctgac catacgcacc atgcctttcg tgcgctggct catggctgag gacagcttcc
     1021 ggtcgtggtc gaggatggcg tcgctgattc tcttctcgct ttcgctcagt tccgctctga
     1081 cagacttctc gtgctcgctg aatgcggctt tcagcatctc gccggtactc tgctgctgcg
     1141 ctttcgattt ctgctctaag tcctttgcca gcgttaaaag actgttcata gatggctccc
     1201 ttcagtcgga tgtttcgccc cccgtccggg tcggcaatgc tgatgctgct ttttgtggtg
     1261 cgtaccacct caaaacctgc gctttccagc gcctcagtga cgtcctggcg ggttttcagc
     1321 tccccggacg aggctaaggc cagtaaaccc cgtgtaattg cctgagcggc ttcctgcttc
     1381 gtttccggca gcgcggacgg cgtgaccagc gcccgccggt tttcaggcgc gttcgggtca
     1441 tgcagcccca gcctgccgtt cacgatggtc tgccaggcat caatgcgcgg acggtcggcg
     1501 cggtcgtaat acggctggag acgcttgccg gtcagcagct ccgtgttcgg gatcaggaag
     1561 ttcagctcca gccgcccctt atcctggtgc tcaacccaca gcacgctgta ctggtcttta
     1621 tcgagtccgg gcatcagaac acgttcaaag ctcgccatca gcgtttcgcg ctgcccgggc
     1681 ggtaaatcct tttcggcaaa ggacaggacg ccggatgtgt acttttttgc gtagggcgag
     1741 gcgtcgataa gctccctgac ttcttccggc ttcccctgca gaacggtggc gccgtcgcgc
     1801 tgtcggtctt tccccagcag ataatccacc ggaccggcgc caccgccgcg ccctcgcggg
     1861 tgaaacttaa cgatcatcgt ctgcaccctt ttccagcaca gcatgccgca gccgctcgag
     1921 tccggcatcg atggccatca gcgcggcgac gacctgcacg cggtcgtgcc cgctgccacc
     1981 accggcgtta acctgccggg caatctggtt gaggttgttc cccatgccgg caagctgacg
     2041 aagcagcgcc ggcgagagcg agggaagctt gccggcgcgg gcgggtttct cgtccaggca
     2101 cgtctggcgc atccaggctg caagctgttt accgtcgcag cgatccagca gacggcggtg
     2161 ctcatcttcg gtcacccaca tcgtgagcat tttgctgcgt ttgtctgcca atccggcctc
     2221 ctgataacgc cgggacgggc tggaaatctt ttcccggtcg gcgcggtgtt ggacaagccg
     2281 caggcgcgtc agtggtttta gcgggtttcg gggcgcagcc ctgaaccagt cacgtagcgc
     2341 tagcggagtg tatactggct taaaatgtaa catcgcagaa acataatgca tagatgccac
     2401 ataaagcagg tgtaatgcca tggcgggcgc gtaagcagaa aatgcaggac cggtgatatt
     2461 ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga gcggtaccgg
     2521 cttacgaatg gggcggaaat ttccggggag atgccaggag ggaacttaac agggaaacga
     2581 gaaggccgcg gcgctgccgt ttttccatag gctccgcccc cctgacaagc atcacagaat
     2641 cggacgctca aatcagtggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc
     2701 cctggtggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg
     2761 ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt aggcagttcg
     2821 ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gactgctgcg ccttatccgg
     2881 taactatcgt cttgagtcca acccggaaag acacgacaaa acgccactgg cagcagccac
     2941 tggtaacagg tttgtgcgag agatgtgttc agagttcttg aagtggcggc ctgactgagg
     3001 ctacactgga aggacagatt tggtgacatg tctcgcacaa gactgttacc acggttagag
     3061 aatctgccag gattaactaa ccttcgaaaa accacctgcc agggtggttt tttcgtttac
     3121 agtcatgaag attacgcgta gaaaaaaagg atctcaagaa gatctttcca tcatttaaca
     3181 atgtcggtat agataaaaat cattcacgaa taaatgaata tccaaaggtg actgtagtga
     3241 acagcaaaca aaaatgttct cattcaaatc tagaaaaaga atactacttg ggtagcgcca
     3301 caggcgatta taaatgtttg gattgcgggg aggttggttt tgggaaggac tggccagaaa
     3361 aagaaagaag taaaaatgat aacgaagcat aaaaaaggcc gctattgcgg ccttttttat
     3421 catttagttt ggcaattgtc tttacatgaa aatgacactt tatctaacat aaccctctca
     3481 ccattagaca ttggcactgc cactttataa tctccgttta agtaaacgaa agtactggac
     3541 ttagcaccaa cataaccgcc ataagagttt tttgcattat atgtgatagt agtgaaccag
     3601 ccatgctctg tagagccaaa gccatactta gaaaaacctc tggcgggggt ggttattgat
     3661 ataactttta gagaatcagg atcttttaga ctctctttaa tcataccaat cactttcgca
     3721 tcattatcta ttggttttga accataatta gcactattta cttcgttctg agaaacacca
     3781 caagcagata ttaacagaac accagacagt gaagcccact taattatttt catatatatc
     3841 cttgtcattt tccaaacaac tataggaaat ccactatata gacatttata gatagattta
     3901 ccataacatc tcttgggcgc accgctcaga cgcgctcagg gcgtttttcc gtcctaagcc
     3961 acaaaagagc ccgtcttctc accgaaaacc gcccggaatc gcgctgataa gttttgcgtg
     4021 tatgcatagc catgcacgaa aatgaactaa cactctgttc aatgaatttt catgctgtac
     4081 gcagtatttt agtcttctat gccccctcga aacacgcctg ttcttgtgta gattttttcg
     4141 acccaagatg gccttttatc agagttaatg cagccataca ggaacacacc tgttttgggt
     4201 ttatagctcc caccctcctg tggctggtta tctacaaatg aataatcaat c
//
