LOCUS       NZ_CP070985             2393 bp    DNA     circular CON 23-MAY-2024
DEFINITION  Staphylococcus aureus strain WBG8366 plasmid pWBG764, complete
            sequence.
ACCESSION   NZ_CP070985
VERSION     NZ_CP070985.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14225473
            Assembly: GCF_017088385.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Karakatsanis,N.M., Colombi,E., Mowlaboccus,S., Coombs,G.W. and
            Ramsay,J.P.
  TITLE     Genome sequence of early Western Australian community associated
            methicillin-resistant Staphylococcus aureus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Karakatsanis,N.M., Colombi,E., Mowlaboccus,S., Coombs,G.W. and
            Ramsay,J.P.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2021) School of Pharmacy & Biomedical Sciences,
            Curtin University, Kent street, Bentley, WA 6102, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 22-FEB-2021
            Assembly Method :: Flye v. 2.8.3-b1695; guppy_basecaller v.
            4.4.1+1c81d62; racon v. 1.4.15; pilon v. 1.23
            Assembly Name                     :: WBG8366-ST78-nanopore-2021
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NextSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_017088385.1-RS_2024_05_23
            Annotation Date                   :: 05/23/2024 04:37:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,790
            CDSs (total)                      :: 2,708
            Genes (coding)                    :: 2,635
            CDSs (with protein)               :: 2,635
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 35 of 73
            Pseudo Genes (incomplete)         :: 37 of 73
            Pseudo Genes (internal stop)      :: 16 of 73
            Pseudo Genes (multiple problems)  :: 13 of 73
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070985.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /strain="WBG8366"
                     /db_xref="taxon:1280"
                     /plasmid="pWBG764"
                     /geo_loc_name="Australia: remote Western Australia"
                     /collection_date="1995"
                     /identified_by="Frances O'Brien, School of Biomedical
                     Sciences, Curtin University of Technology, Perth, Western
                     Australia"
                     /note="ST78-MRSA-IVa (2B); screening"
     gene            40..962
                     /locus_tag="JX573_RS13865"
                     /old_locus_tag="JX573_13855"
                     /pseudo=""
     CDS             40..962
                     /locus_tag="JX573_RS13865"
                     /old_locus_tag="JX573_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002499696.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            1458..1640
                     /locus_tag="JX573_RS13870"
                     /old_locus_tag="JX573_13860"
     CDS             1458..1640
                     /locus_tag="JX573_RS13870"
                     /old_locus_tag="JX573_13860"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000877306.1"
                     /translation="MKYRIIAALFFLMLLLIYVFKIAPFLNTDSQIVNLVVVLIIFFIG
                     ALLGWISRKFDKNSK"
                     /name="hypothetical protein"
     gene            1690..2133
                     /locus_tag="JX573_RS13875"
                     /old_locus_tag="JX573_13865"
     CDS             1690..2133
                     /locus_tag="JX573_RS13875"
                     /old_locus_tag="JX573_13865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012816707.1"
                     /translation="MYKKILTLSILLVMIFTVVPVHNEVEAKPKKGSVVTINKKQYIYQ
                     GTVKKYYPAKYCKLVNKYAKNSQSFTNLVAGTSGFATFAKKAGYAGVLASSLYIANLGV
                     QNNTTIYKNAARKGTGVQVSYDLYVPKTGHNYGLTRNTKVVYK"
                     /name="hypothetical protein"
     Typing          2..962
                     /name="rep_cluster_1130"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.586"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgacacata ataatataaa caatcgtaaa atacaagata ttgagagata tgagagtaaa
       61 aaggttagaa gtgaagagtt tagagattta gtaaaaagtt tagtatctga ttcgtattat
      121 tggttaattg aggaatgtgg gacattctta tcgtttgttg gtgataaaga attaaacaaa
      181 attaaaatgc atcgtgggaa tttttgtaag catagatttt gtccaatgtg tgcatggaga
      241 aaagcgagaa aagatgcttt ggaattatct ttaatgatgc aggatataaa gattagttat
      301 gataaagcat ttatcttttt gacgcttaca acaccgaacg tagtagctga aagattggga
      361 gatgagatta aaagatataa taaatctttt gataaattat ttaaacgaaa agttgtaaaa
      421 agagtagtaa aaggttatgt tcgaaaatta gaaataactt ataatgagaa acgagatgat
      481 tatcacccac attttcatgt gcttattgtt gttaataaat catattttac ggatgtaaaa
      541 gcttatttga atcaaaaaga gtggttgcgt ttatggcgtg aggcaacagg aatttcagaa
      601 attacgcaag tagatgtgcg tcgggtcaaa gagcaaaata gtaatgagtt gtatgaaatg
      661 gctaaatata caggtaaaga tagtgattat ttatctaatg aaaaagtgtt taaagttttt
      721 tatgagtctt taaaaggtaa aagggcattt gcatatggtg gattatttaa agaagttaga
      781 aaaaagctta aaaatggcga tttagattat ttaaaagagg ttgatccaac aaaatacata
      841 tatcaaattt tttgtggttg gaatcaaatg cattattatg cgagtgaatt gagattgatg
      901 agtgaagaaa tgcaaaagtg aatcatcaaa tgataaatga attagatgat aaatatttat
      961 aagagcttta tttttcaatt ttatatataa agaattgcat atgtaattta gtgtagtctg
     1021 aggtgctgac agagtcagca aggcggagcc gtccagcgga gcgttcagac aagtaatata
     1081 aaatgtgtag gcggagtttt agaattaagt aaaagaattt gtttttgaat gtatttaaag
     1141 ccattaaaaa gtattaaact aattttggac gtataaatgt atcgattagt tattttgttt
     1201 taaaatatgc ttaaaatgcg aaataatcat atattcacat ttatgccgag aaaacatttt
     1261 gaaaaagaaa aaagctatat gatatttcaa cttgttgaaa atgttggtgg ggtttgaatt
     1321 gggattgatt ccaagaaaac accaacagaa aatcatatag cttaaagttt ctgttcaaaa
     1381 tggaaattgg aataaattta tggtttattg tataagaatg aatatagaat ttattaagaa
     1441 agattaggag gatttatatg aaatatagaa ttatagcggc tttgtttttt ttgatgttgt
     1501 tattaatata tgtatttaaa atagctcctt ttttaaatac tgatagtcaa attgtaaatt
     1561 tagttgttgt gttaattatt ttctttatag gtgcattatt agggtggatt agtcgtaaat
     1621 ttgataaaaa ctccaaataa atatattttt gtgttatatt aatacaaaaa tatattaggg
     1681 gtgtatttta tgtataaaaa aatattaact ttatctattt tgcttgttat gatttttaca
     1741 gttgttcctg ttcataatga agttgaagct aaacctaaaa aaggttctgt tgtaacgatt
     1801 aataaaaagc aatatatata tcaagggact gtaaaaaaat attatcctgc aaaatattgt
     1861 aaactggtta ataagtatgc taagaattcc cagtctttta caaatttagt ggctggtact
     1921 agcggttttg cgacttttgc aaaaaaagct ggttatgctg gtgtgttagc ttcgagttta
     1981 tatattgcta atttaggtgt tcagaataat actacgattt acaaaaatgc tgcaagaaaa
     2041 ggtacgggtg tgcaagttag ttatgattta tatgttccta aaacaggaca taattatgga
     2101 ttaacacgta atacaaaagt tgtatataaa taaaaaaatg gagatgtttt tcatctccat
     2161 ttttttattt tcttctatct tgataatata ggtaacaact cacggatgtc tgtataggca
     2221 tctaaccctt gataataaag gaaaaaacat agaaaaactt gctttatcca gcatattatt
     2281 ctagaatttt aggtgtctaa tcaaaaattc taaatattgg aggtataaag caagtttttt
     2341 gttcgtaata tttttgtatc caaatttata gcgtcttaaa atttgttaat tac
//
