LOCUS       NZ_CP070901            21383 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain JNQH116 plasmid pJNQH116-3, complete
            sequence.
ACCESSION   NZ_CP070901
VERSION     NZ_CP070901.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17976248
            Assembly: GCF_017084505.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 21383)
  AUTHORS   Hao,M.
  TITLE     Complete genome sequences of a hypervirulent and carbapenem
            resistant klebsiella pneumoniae strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21383)
  AUTHORS   Hao,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-2021) Department of clinical laboratory medicine,
            The First Affiliated Hospital of Shandong First Medical University,
            Jingshi Road, Jinan 250014, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.14; Unicycler v. v0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 98.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:42:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,521
            CDSs (total)                      :: 5,395
            Genes (coding)                    :: 5,238
            CDSs (with protein)               :: 5,238
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 80 of 157
            Pseudo Genes (incomplete)         :: 92 of 157
            Pseudo Genes (internal stop)      :: 31 of 157
            Pseudo Genes (multiple problems)  :: 44 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070901.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..21383
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="JNQH116"
                     /isolation_source="cerebrospinal fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pJNQH116-3"
                     /geo_loc_name="China"
                     /lat_lon="36.66 N 116.99 E"
                     /collection_date="2020-04-20"
                     /collected_by="Mingju Hao"
     gene            1..1011
                     /locus_tag="JYA99_RS27350"
                     /old_locus_tag="JYA99_27350"
     CDS             1..1011
                     /locus_tag="JYA99_RS27350"
                     /old_locus_tag="JYA99_27350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900946.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_004211839.1"
                     /translation="MTSENNSLPLNLQEVDKTTGEVVKLDVNSTSTVQPVALMRLGLFV
                     PTLKSTSRSKANRKNVTDATEELVQLSIAKSEGYTDVKITGSRLDMDTDFKVWLGIIRS
                     MSEYGVKSDTLELSFVEFVKMCGFDSRRSNKKMRDRISNSLFKLASVTLQFQSETKGWT
                     THLVQSAYYDINEDIVEIKAEPKLFELYHMDRRVLLRLKAIDALQRKESAQALYTYIES
                     LPQNPAPISMKRMRDRLNLTSNVYTQNHTVRKAMEQLRDIGYLDYTEFKRGRATYFSVH
                     YRNPKLISGTVKVPRKEEEEKAPEQNYDEVIKALKAAGIDPQKLAEALSAMKPEN"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(1833..2573)
                     /locus_tag="JYA99_RS27355"
                     /old_locus_tag="JYA99_27355"
                     /pseudo=""
     CDS             complement(1833..2573)
                     /locus_tag="JYA99_RS27355"
                     /old_locus_tag="JYA99_27355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540156.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA];
                     GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /name="site-specific integrase"
     gene            <2867..3140
                     /locus_tag="JYA99_RS27360"
                     /old_locus_tag="JYA99_27360"
                     /pseudo=""
     CDS             <2867..3140
                     /locus_tag="JYA99_RS27360"
                     /old_locus_tag="JYA99_27360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611340.1"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(3277..3813)
                     /locus_tag="JYA99_RS27365"
                     /old_locus_tag="JYA99_27365"
     CDS             complement(3277..3813)
                     /locus_tag="JYA99_RS27365"
                     /old_locus_tag="JYA99_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004117808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004211835.1"
                     /translation="MENINNDVPQHQPYRNEKVFNSGKTALEFNFSETNGSVNLILAGP
                     LVSKPGSFDWTGQKAFSTKLSDDEVITLCMAFLRLTHEAVLKDKKTKHHNKQVYKNVKV
                     TFDGKSTAMMEGGVVAINKDERDINFIHKISIDPAACLRLGLFLLSVILARNPGVPSDA
                     VLTCMRLNANAQLQK"
                     /name="hypothetical protein"
     gene            complement(4024..5092)
                     /locus_tag="JYA99_RS27370"
                     /old_locus_tag="JYA99_27370"
     CDS             complement(join(4024..4870,4869..5092))
                     /locus_tag="JYA99_RS27370"
                     /old_locus_tag="JYA99_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611505.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_223271235.1"
                     /translation="MAFALKQAETGTRVEEVCRKMGISEATFYNWKKKFGGMGVTELRR
                     LRQLEEENQCLKRLVADLSLDKEMLQEVIKKKVLRPAQKRQAVHFLREAYRISVRRGCG
                     LLMQSRTVYHWQSRRDDRAITLRIREIAEPRIRYGCPRIHIQLRREGWPVNHKKTHRIY
                     CLEGLNLRKKRPRRHVSAAHRQQRPVLTGVDQCWSMDFVSDNLFNGRRFRALTVVDNFS
                     RECVAIHAGKSLKGEDVVGVMERLRVPGKRLPVRIQTDNGSEFISKSLDKWAYEHGVTM
                     DFSRPGKPTDNPFIESFNGSLRDECLNIYWFLSLEDAQDKLDNWRREYNHERTHSSLND
                     MTPAELIRSLRKDEAL"
                     /name="IS3 family transposase"
     gene            5545..6567
                     /gene="istA"
                     /locus_tag="JYA99_RS27375"
                     /old_locus_tag="JYA99_27375"
                     /pseudo=""
     CDS             5545..6567
                     /gene="istA"
                     /locus_tag="JYA99_RS27375"
                     /old_locus_tag="JYA99_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /name="istA"
     gene            6564..7346
                     /gene="istB"
                     /locus_tag="JYA99_RS27380"
                     /old_locus_tag="JYA99_27380"
     CDS             6564..7346
                     /gene="istB"
                     /locus_tag="JYA99_RS27380"
                     /old_locus_tag="JYA99_27380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSen3 family helper ATPase
                     IstB"
                     /protein_id="WP_004214667.1"
                     /translation="MMVELQHQRLMVLAEQLQLDSLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGVPQKQIQSLRSLSFIERNE
                     NIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTVQRQGRYKTTLHRGV
                     MAPKLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDTALT
                     SAMLDRILHHSHVVQIKGESYRLKQKRKAGVIAEANPE"
                     /name="istB"
     gene            complement(<7412..8061)
                     /locus_tag="JYA99_RS27385"
                     /old_locus_tag="JYA99_27385"
                     /pseudo=""
     CDS             complement(<7412..8061)
                     /locus_tag="JYA99_RS27385"
                     /old_locus_tag="JYA99_27385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611474.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(8130..8453)
                     /locus_tag="JYA99_RS27390"
                     /old_locus_tag="JYA99_27390"
     CDS             complement(8130..8453)
                     /locus_tag="JYA99_RS27390"
                     /old_locus_tag="JYA99_27390"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RES domain-containing protein"
                     /protein_id="WP_004214672.1"
                     /translation="MPVVPELEETNYLLTQAIADYLSTFEHCELDGILFASAQKPKEST
                     DPSVMNIILFHKSSGVLNADRRFREAEVNMYQHDEDISYFDPEIRTTPDDFRREFKVLS
                     RKK"
                     /name="RES domain-containing protein"
     gene            8597..9565
                     /locus_tag="JYA99_RS27395"
                     /old_locus_tag="JYA99_27395"
     CDS             8597..9565
                     /locus_tag="JYA99_RS27395"
                     /old_locus_tag="JYA99_27395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="WP_004225014.1"
                     /translation="MKDQITHPPDNSDHSVAKQKFRITNWSTYNKALINRGSLTFWLDD
                     EAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFALMNVPLR
                     CPDYTSVSKRAKSVNVSFKTSTRGEIAHLVIDSTGLKVFGEGEWKVRKHGKERRRIWRK
                     LHLAVDSNTHEVVCADLSLNNVTDSEAFPGLIRQTHRKIRAAAADGAYDTRLCHDEMRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRLSGSNARWKWTTEYNRRSIAETAMYRMK
                     QLLGDSLTLRDYDGQVAEAMAMVRALNRMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(9610..10119)
                     /locus_tag="JYA99_RS27400"
                     /old_locus_tag="JYA99_27400"
     CDS             complement(9610..10119)
                     /locus_tag="JYA99_RS27400"
                     /old_locus_tag="JYA99_27400"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_225314228.1"
                     /translation="MSRILTGICETDPARRLYKVAVSRGNTFLSAKERFRVQVCFKNAT
                     THPDEDRLLAELLTVHHFLADTSILWHMEQKTYACTTTWATTSTWLPEIYQRQASTPNV
                     VKIIKFMHARFPGLKTVAETNEQGYEQALTSVDFEIPYWPVLPKFESDIGTIQVTIAGM
                     IRYRLC"
                     /name="hypothetical protein"
     gene            complement(10116..11075)
                     /locus_tag="JYA99_RS27405"
                     /old_locus_tag="JYA99_27405"
     CDS             complement(10116..11075)
                     /locus_tag="JYA99_RS27405"
                     /old_locus_tag="JYA99_27405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011251302.1"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="WP_004026549.1"
                     /translation="MNTDFAHYNEEQLRKLGELHSLLRHSDIGSSYLATLPEPRSVEEL
                     NPPQEINVTHSVPDVDTLVDIYRQQRVDKVHVRDEHYSTKITRKYPGFVVVRNNHDQVM
                     SLVGEINRLRDKFADAVKAITHYQDSRSEILHQVYPWLVTLQVSRNIRIVTEQIRSLGF
                     TWQIPVIHKFTRLETVIDRLRREITELQPDISLTKQDVERLKQERTEEIMMLSLLNDEV
                     SHDDENLRKFRLIRESNFPAVNVNIRYTSPEDPDDVHGPYKLNFRAPLPLILFSEPGRF
                     NPLKNYVKGERQKRGDFNEKYRSVLPRIGLVEVIRESK"
                     /name="DNA replication terminus site-binding protein"
     gene            complement(11118..11525)
                     /locus_tag="JYA99_RS27410"
                     /old_locus_tag="JYA99_27410"
     CDS             complement(11118..11525)
                     /locus_tag="JYA99_RS27410"
                     /old_locus_tag="JYA99_27410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004186937.1"
                     /translation="MSKVHCISGITWKNSDSASFKVYASIAAVFSLGLVTATVRNYEIL
                     QLTVPYTWYVFFTAFWLLFPSFTVSFREREGRLFTSMGFYGLKLFTLDRPVSDGYWLVT
                     MEKNRYRLVRVCRDGRRQPVWLTFKSLPEFV"
                     /name="hypothetical protein"
     gene            complement(11535..11978)
                     /locus_tag="JYA99_RS27415"
                     /old_locus_tag="JYA99_27415"
     CDS             complement(11535..11978)
                     /locus_tag="JYA99_RS27415"
                     /old_locus_tag="JYA99_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004213821.1"
                     /translation="MILKEEIVLGIYSWLHMTPVSMLVRNITSDQGGDYAIVRFTVDSR
                     GGQMGPKAQGQLLCSFGFNVKESCEADPKDGPGLIKAEMMNGVMQLVPECIELTDSQTQ
                     AIRKEVTVFNRVCAMQLLGGHGNARSLWEKEILPRMKVRRQLH"
                     /name="hypothetical protein"
     gene            complement(11999..>13074)
                     /locus_tag="JYA99_RS27420"
                     /old_locus_tag="JYA99_27420"
                     /pseudo=""
     CDS             complement(11999..>13074)
                     /locus_tag="JYA99_RS27420"
                     /old_locus_tag="JYA99_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            13242..14210
                     /locus_tag="JYA99_RS27425"
                     /old_locus_tag="JYA99_27425"
     CDS             13242..14210
                     /locus_tag="JYA99_RS27425"
                     /old_locus_tag="JYA99_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_004213807.1"
                     /translation="MSQPNPLCVGIDVSKATLDIAASSDVAQFTVSNDSDGFNAIITGL
                     RRHSVALVLMEATGGLEVAVACSLQAEGFEVAVVNPRQARDFARAMGYLAKTDRIDARV
                     LTQMAEVINRHPERKRFIRALPDAERQALAAMVVRRRQLIAMLVAERNRLHPFHPQNKK
                     SINTIIKALEDELVRLEKEMDSHIRNHFKEIAERLSSIKGVGTMTVSAMLAEIPELGTL
                     SRREISALIGVAPVNRDSGTMRGRRTIFGGRAGVRSALYMAALVATRFNPVIKTFYVRL
                     LAAGKAKKVALVACMRKLLTILNAMLRKNEEWDESYHHVAP"
                     /name="IS110 family transposase"
     gene            complement(<14229..14393)
                     /locus_tag="JYA99_RS27430"
                     /old_locus_tag="JYA99_27430"
                     /pseudo=""
     CDS             complement(<14229..14393)
                     /locus_tag="JYA99_RS27430"
                     /old_locus_tag="JYA99_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(14479..16071)
                     /locus_tag="JYA99_RS27435"
                     /old_locus_tag="JYA99_27435"
     CDS             complement(14479..16071)
                     /locus_tag="JYA99_RS27435"
                     /old_locus_tag="JYA99_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISKox1 family transposase"
                     /protein_id="WP_004902302.1"
                     /translation="MNHDYLARIAALEDALRQKDSQLSLVAETESFLRSALARAEEKIE
                     NEEREIEHLRAQIEKLRRMLFGTRSEKLRRQVEEAEALLKQQEQQSDRYNGREDDPQVP
                     RQLRQSRHRRPLPAHLPREIHRLDPAETSCPECGSGMAYLSEVSVEQLELVSSALKVIR
                     TVRVKKACTRCDCIVEAPAPSRPIDRGIAGPGLLARVLTAKYCEHLPLYRQCEIFARQG
                     VDLSRALLSNWVDACCRLMAPLDEALYHYVMDCHKLHTDDTPVPVLAPGRKKTKTGRIW
                     TYVRDDRSAGSSDPPAAWFAFSPDRQGKHPQQHLRHYHGVLQADAFAGYDRLFSPEREG
                     GPLTEAACWAHARRKIHDVYISTHTATAEEALKRIGELYAIEEEIRGLTTEERLAARQS
                     QSKPLLASLHEWLVEKNETLSKKSRLGEAFAYVLNQWDALCYYCEDGLAEPDNNAAERA
                     LRAVCLGKKNFIFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNKV
                     AELLPWNVVLTDK"
                     /name="IS66-like element ISKox1 family transposase"
     gene            complement(16102..16452)
                     /gene="tnpB"
                     /locus_tag="JYA99_RS27440"
                     /old_locus_tag="JYA99_27440"
     CDS             complement(16102..16452)
                     /gene="tnpB"
                     /locus_tag="JYA99_RS27440"
                     /old_locus_tag="JYA99_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="WP_004189161.1"
                     /translation="MISLPSGTRIWLVAGATDMRKSFNGLGEQIHHVLDEDPFSGHLFI
                     FRGRRGDTVKILWADADGLCLFIKRLEEGQFVWPAVRDGKIAITRSQLAMLLDKLDWRQ
                     PKISRLNSLTML"
                     /name="tnpB"
     gene            complement(16449..16889)
                     /locus_tag="JYA99_RS27445"
                     /old_locus_tag="JYA99_27445"
     CDS             complement(16449..16889)
                     /locus_tag="JYA99_RS27445"
                     /old_locus_tag="JYA99_27445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_004215130.1"
                     /translation="MENAANWRTESRTVYSSDFKLRMVELASRPDANVAQLAREHGVDN
                     NLIFKWLRLWQREGRISRRMPATIVGPVVPQSLPASPILLSVDVINDPLPAAENDTLCT
                     FSSAHASATSCHVEFRHGKMTLENPSSELLTVLIRELTGRTQ"
                     /name="transposase"
     gene            <16974..17268
                     /locus_tag="JYA99_RS27450"
                     /old_locus_tag="JYA99_27450"
                     /pseudo=""
     CDS             <16974..17268
                     /locus_tag="JYA99_RS27450"
                     /old_locus_tag="JYA99_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152715.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /name="DUF4113 domain-containing protein"
     gene            17347..17575
                     /locus_tag="JYA99_RS27455"
                     /old_locus_tag="JYA99_27455"
                     /pseudo=""
     CDS             17347..17575
                     /locus_tag="JYA99_RS27455"
                     /old_locus_tag="JYA99_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            complement(18517..19488)
                     /locus_tag="JYA99_RS27465"
                     /old_locus_tag="JYA99_27465"
     CDS             complement(18517..19488)
                     /locus_tag="JYA99_RS27465"
                     /old_locus_tag="JYA99_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition protein"
                     /protein_id="WP_004117790.1"
                     /translation="MKRAPVIPRHTTHTQSTEDTSSPAPAAPMVDSLIARVGAMARGNA
                     ISLPVCGREVKFTLEVLRGDSVESASRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSDVIEIADGSRRRKAAILTESDYRILVGELDDEQMAALSRLGNDYRPTSAYER
                     GLRYTSRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFAHPGELSARSGE
                     ALQKAFADKEELLKQQAETLHDQKKAGLIFEAEEVISLLTSVLKQSSASRVNLSSRHQF
                     APGATALYKGDKMVLNLDRSRIPAECIEKIEAILKELEKLGV"
                     /name="ParB/RepB/Spo0J family plasmid partition protein"
     gene            complement(19488..20654)
                     /gene="sopA"
                     /locus_tag="JYA99_RS27470"
                     /old_locus_tag="JYA99_27470"
     CDS             complement(19488..20654)
                     /gene="sopA"
                     /locus_tag="JYA99_RS27470"
                     /old_locus_tag="JYA99_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="WP_004211841.1"
                     /translation="MGLMDILNQCITAGHEMTKAIAIAQFNDDSPEARKITRRWRIGEA
                     ADLVGVSSQAIRDAEKAGRLPHPDMETRGRVEQRVGYTIEQINHMRDVFGTRLRRAEDA
                     FPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDLHIH
                     AEDTLLPFYLGEKDDASYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTDPHL
                     MLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFFDML
                     RDLLKNVDLKGFEPDVRILLTKYSNNNGSQSPWMEEQIRDAWGSMVLKNVVRETDEVGK
                     GQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCDEIFDRLIKPRWEIR"
                     /name="sopA"
     Typing          2..1011
                     /name="IncFIB"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.604"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgacgagcg aaaataacag cttacctctc aatcttcagg aagtggacaa gacaaccggc
       61 gaagttgtta agctggatgt caacagtacc agtactgtgc agccggtagc gctcatgagg
      121 ctcggtctct tcgttccaac cctgaaatca acctccagga gtaaggcaaa ccgtaaaaac
      181 gtcacagatg ccactgagga gctcgtacag ctgtccatcg ccaaaagcga agggtacacc
      241 gacgttaaga tcaccggttc gcgtcttgat atggacacgg attttaaagt ctggctcggg
      301 atcattcgct cgatgtctga gtatggggta aaaagtgaca ccctggaact gtcgttcgtt
      361 gaattcgtta agatgtgtgg atttgactca cggcgctcaa acaagaaaat gcgggatcgc
      421 atcagcaatt ccctgtttaa actcgcctcg gttaccctgc agttccagag cgagaccaaa
      481 ggatggacca cccatctggt gcagtcagcc tattatgaca tcaacgagga tatcgtggag
      541 atcaaggctg aacccaagct ctttgagctt taccatatgg acaggcgggt cctgcttcgg
      601 ctgaaagcca ttgacgccct gcagcggaag gaatctgccc aggcgctgta tacctacatc
      661 gagagccttc cccagaatcc cgcacctatc tccatgaaac gtatgcggga ccggctgaat
      721 cttacctcaa atgtctatac gcagaaccac acggtccgaa aggctatgga gcaacttcgg
      781 gatatcggct atctggatta caccgagttt aaacgtggcc gggcgactta ctttagcgtt
      841 cattatcgca atcccaaact catcagcggg acggtgaagg taccgcgcaa ggaggaagaa
      901 gagaaggcgc cggagcagaa ttatgacgaa gtcatcaagg cactgaaggc tgcaggcatc
      961 gatccgcaga aacttgctga agcgctatcg gcgatgaagc cggaaaattg actgcgatat
     1021 gcattaccat gactgccgta tgcatacgca attagttatt gactgtgagg tgcatcacgc
     1081 atactacagt caatattcgc tctccttcct gcaggttaag atcggacaat attgactgcg
     1141 gtatgtcatt tatggccaga atatgctgat attgactgca gcatgcaagt ttgacgaggg
     1201 tgttacccga ccaattgaga gctctccaca tgactgcatc ccacagtcaa tatttcagta
     1261 aatccccggt gcgcactgaa tgaagccact cagcagagcg ttgattccgg cattgccata
     1321 caagagctgc taaaaccagt cctccgaagt tcgttaatcc tgtcgtaccc tcagccagct
     1381 tcgcactgat atctgcaatc atgaagagga aacctgatgg ctgtcagaaa tggcccggga
     1441 cgcagtcacc gacgatatac tgaataacga cctggcttac ccagttggaa aggccctcag
     1501 gtatatcccc tgtaacctgc tgatatccgg atcgcagtaa gttttattgt ctgcggtatg
     1561 aagatgtcag ctggtcacca gaggaccggg gctccttaat acgaatgatg tcatggtgca
     1621 atgagaaggt gaggggacac gggatgcggt atgaatattt ctctccagac ccggatggaa
     1681 tgatgcccgg gtcatactgg ataagcgtaa cgcttctgca gccggcacgg tgagagactc
     1741 agaagcgact gactgactct gcctcgtctg gaatgcgttt gtctttatct ccttcactaa
     1801 gtggcaaagc cccggccgcc tgcccgacaa tctcagccgg ccggtggcag ggagcgaagg
     1861 atctccgcgg catctcgacc gtcagcggta aagggaacgg ccagggtggc agccatgtcc
     1921 agcgcaaata cccgggtata gacttccatc gagcgcggat ccttatgccc ggccagcgcc
     1981 tggatgacct tcctgagctg ccggtgataa agcatgtgca tgatatagct gtgccggaag
     2041 gtatgcggcg tcacggcgat cgaaaaatgc acgccatcag cttcagcccg cttcaccgcc
     2101 tgtttcagcc agttgcgcat tgtctcatcc gtgaccggcc ataacggctc ccgccggcgg
     2161 ggcgggtggt gaccatccag ctttccatct gacgcacaaa actgccatcc gtcagcggca
     2221 ccaggcgcac ttcatctttt ggcggccgcc cgcggcgcgc ccgcactttc tccgaaagca
     2281 ccctaacaaa agggcgcaga ccatccaggt cgaaggattc cggggtgagc gtccgggctt
     2341 caccgatacg gctcccggta ttccacatgg tggcgaacaa catgtggtga cgctggtccg
     2401 gcatatacca gaggagggcg ctcacttccg gcgccaggag gtaagccggc gtggcacctg
     2461 ttgaggtggc catccgccgg agtgccaccg cggtcgcaaa atcaacgccc ggggcggatc
     2521 ggcagcaggg agacgcgatc tggtgaattc tgcaggggaa tgatgctgtt catgagttat
     2581 ttttcacgta gcgcacaacg ggtttggata cagctattct ggcctgaagc ggctgatcct
     2641 gccatgacaa taaatcgcag cgcgcggatc cttttgcggt taagatctat gcttaagcca
     2701 gttatgacgc ggcctgcagg cactttcgtc gtttttggga gaaatggggt aatgctagga
     2761 caaacagcta tgttgacact ggcagtaatg gagacgagaa acggctacct tccagatgcg
     2821 atcggcagga atgatcttca acatgttccg tctgtggttg tgtaagggta gtcagtacgg
     2881 cagtgacgac tggcaccgct tctgcgggcc aataacctgg caccgagctt gagccggcgt
     2941 ggcaactgcc aggataatgc ggtggccgaa ttgttcttca gttcactgaa aaaagaacgc
     3001 attagtaaga gaacatacca aaccgggatc tggcccgggc ggatgtcttc gattacattg
     3061 aagtgttcta caatcggacc cggcgccaca gtcatcccgg cggcgtcagt ccggaggcct
     3121 tcgaacaggc ctcgtcgtga ggacagaatt tgtctacagg catggggtca gtccatagtt
     3181 acatctaatg tatcctatga accgagcgta ttttcaccat taagggaagt atcttttttg
     3241 aaattcccgt aaaacgggaa tgataagata gaaaagttat ttttgtagtt gagcatttgc
     3301 attaagacgc atgcacgtta gaaccgcgtc agatgggacc cctggatttc gggccagtat
     3361 gacggacaag aggaaaagac ctaagcgaag gcaagcggca ggatcaatac taattttatg
     3421 tataaaatta atgtctctct cgtctttgtt gatagctaca actcccccct ccatcattgc
     3481 tgtggattta ccatcaaaag taacttttac gtttttgtag acttgcttat tatggtgctt
     3541 tgttttttta tctttcagaa cagcctcatg agtaaggcga agaaacgcca tacacaaggt
     3601 gatcacttca tcatcactca gctttgttga aaaggccttt tgaccagtcc agtcaaaact
     3661 tcccggcttc gatactaaag gccccgcaag gataagattc acactgccat ttgtctcaga
     3721 aaaattgaac tcaagtgctg tcttaccgga attaaatacc ttttcatttc ggtaaggttg
     3781 atgctgtggt acatcgttat ttatattttc caccaaatat gtccttcacc ttcgttgatt
     3841 aaattcttca ccagaccaat gtaggtatac cctctaggtt gcttcatcgc aaaaatataa
     3901 gcactggtct ccagcattag atagtctcct gagggagcac caggaggaac ctctgtagag
     3961 ctaaggcttt tagtgtgctc ctttttaact ttgaccctgg cccaaattca gtgcagtgct
     4021 aaatcagaga gcttcgtctt tccggagact tcggattaat tcagccggag tcatgtcatt
     4081 taatgatgaa tgcgttctct catgattata ttccctgcgc cagttgtcga gtttgtcctg
     4141 cgcatcttcc agtgagagaa accagtaaat gttcaggcat tcatcccgca gactgccgtt
     4201 aaatgattca ataaacgggt tatccgttgg ttttccgggg cgggagaagt ccattgtgac
     4261 accgtgctca tacgcccatt tatccagact tttcgagata aactcgctgc cgttatctgt
     4321 ctgaatacgt accggcaggc gcttgcccgg cacccgcaac ctttccatca caccgaccac
     4381 atcctcacct ttcagcgact ttccggcatg gatcgccaca cactcccgac taaaattatc
     4441 cactacagtc agcgcccgga aacgtcgtcc gttaaacagg ttatccgaca cgaaatccat
     4501 actccagcac tgatcgactc cggtcaggac cggacgttgc tgacgatgtg ctgcactgac
     4561 atgcctgcgg gggcgctttt tgcgcaggtt gaggccttcc agacagtaaa tccggtgtgt
     4621 tttcttgtgg ttaacaggcc agccttcccg ccgcagctga atatgaatac gcgggcaacc
     4681 gtagcggatc cggggttccg ctatttcccg gatacgcagg gttatcgccc gatcatcccg
     4741 ccggctctgc cagtggtaaa cggttctgct ctgcatcagc agtccgcatc cccgccggac
     4801 actgatacgg taagcctccc gcagaaaatg taccgcctga cgcttctgag ccggcctcag
     4861 aacttttttt gataacttcc tgcagcatct ccttgtccag acttaaatca gccaccagac
     4921 gtttcagaca ctgattctct tcctcaagct ggcgcaggcg gcgcagctct gtcacgccca
     4981 tgccgccaaa ctttttcttc cagttgtaaa atgtggcttc agaaatgccc atctttcggc
     5041 agacttcctc cacccgagtg ccggtttcgg cctgtttcag cgcaaatgct atctgttctt
     5101 aggtatagcg ggtctttttc atggcgatgt gcctctccgt gagatggaag aaagaccgga
     5161 gacttcagtt tagcctagca ctgttttcag ggaggagatc agatctttgg taaaggtggc
     5221 tgttccaatg ttctttaccc tacctgtgac cattaatcgg caagttctga agtgattagt
     5281 agccctgaat tcgttgtcct ggaaacgcct gtaaacctct tcaggagtat gtatccgatt
     5341 aacaacgttg accatttccg gttctgctgg ttttagaggt acttgagtta acatctggag
     5401 tttctgtgtc atggttcgga ggtgatgtaa ggacaccatg aacaacaagc actgcaaatg
     5461 ttaccaacgg tgcgaaagtg atccatatcc gacgcctgaa attagatcca ggggttaatc
     5521 tgctctcctg attcgggaga gcttatggtc acttttgaga cagttatgga aattaaaatc
     5581 ctgcacaagc agggtatgag cagtcgggcg atagccaggg aactgggtat ctcccgtaac
     5641 accgtaaagc gttatctgct tgcgcagtct gaaccgccaa aatatacgcc gcgttccgcc
     5701 gtcgcttcac tcctggatga acaccgggac tatattcggc aacgcatcgc cgatgcccat
     5761 ccttataaaa tccctgcaac ggtcatcgcc cgcgagatca cggagcaggg ttatcgtggt
     5821 ggcatgacca tcctgagaga gttcatacgt tcactcgcca tccctcagga acaggagccc
     5881 gtcgtgcgct tcgaaacaga accaggacga cagatgcagg ttgactgagg caccatgcgt
     5941 aatggcaaat caccacttca tgtgtttgtt gccgttctgg ggttcagcag aatgttgtat
     6001 atcgagttca ctgacaatat gcgctacgac accctggaag aatgccaccg taatgcgttc
     6061 agcttcttcg gtggcgtacc gcgtgaagtg ctctacgaca atatgaaaac ggtagtgtca
     6121 caacgcgatg cctatcggac cgggcaacac cggttcaacc cttcactgtg gcagttcggc
     6181 aaagagatgg gcttctctcc acgactgtgc cgccccttca gggcacagac taaaggcaag
     6241 gtggagcgga tggtgcagta cacccgcaac agcttctaca tcccgctaat gacccgcctg
     6301 cgtccgctgg ggatcaccgt cgatgttgaa acagccaacc gctacggcct gcgctggttg
     6361 cacgatgttg ctaaccagcg caggcacgaa acaatccaga cccgtccctg cgatcgctgg
     6421 atcgaggaac agcagtccat gctggcgcta ccgccggaga aaaaacagta tgacgtgctg
     6481 gtagacgaaa gcctggtgac cttcgacaga cagccactgc atcacccgct gtccatctat
     6541 gacacgttct gcagaggggc cgcatgatgg tggaactgca acatcaacga ctgatggtcc
     6601 ttgccgaaca gctgcagctg gacagcctta tcagcgccgc acccgctctg tcgcaacagg
     6661 ctgtggatca ggaatggagc tatatggact tcctggagca tctgcttcac gaagagaaac
     6721 tggcccgcca ccaacgtaaa caggcgatgt atacccggat ggcggccttc ccggcagtga
     6781 aaacgttcga ggagtatgac ttcaccttcg ccaccggtgt cccacagaag caaatccagt
     6841 cgctgcgttc actgagcttc atagagcgca acgaaaacat cgtgttgctg gggccatccg
     6901 gcgtggggaa aacccatctg gctatagcga tgggctacga agcggtacgg gcgggcatca
     6961 aggttcgctt cacaacggcg gcggatctgt tgcttcagtt gtcaacggta cagcgtcagg
     7021 gccgttataa aacaacgctt caccgtggcg tgatggcacc aaagctgctc atcattgatg
     7081 aaataggcta tctgccgttc agtcaggaag aggcaaagct gttcttccag gtcatcgcca
     7141 aacgttatga gaagagcgcg atgatcctga cctccaacct gccgttcggg cagtgggatc
     7201 agacgttcgc cggtgatacg gcgctgacat ccgcgatgct ggaccggatc ttacaccact
     7261 cacatgttgt tcaaataaaa ggagagagct atcgactgaa acagaaacga aaggccgggg
     7321 ttatcgcgga agctaatcct gagtaaaacg gtggatcaat attggaccgt cggtggagat
     7381 gtaagtggat cactttttac ccgtcgttga cagccagcgc gtccatgaca aaatccatcg
     7441 accaggtcag gttgggcgcc tccgggcgga gcagtggcag acgttctgtt gccagccctt
     7501 tacgacgtcg tctgcgtttt acgcccagcc cgttaaggtg gtaaagacgg tacacgcgct
     7561 tgtgattaac atgaaggcct tcacggcgca gtaactgcca gatgcgtcgg tagccaaaac
     7621 gcctgcgctc cagtgccagc tcagtgatgc gccctgataa atgcgcatca gccgccggac
     7681 gctgagcctc atagcggcag gtcgacaggg acaaacctgt aagcctgcag gcacgacgtt
     7741 gcgacagacc ggtcgcatca cacataaact caacggcttc ccgcttctgg tctgtcgtca
     7801 gtactttcgc cccagagcca cctgaagtgc ctccttatcc agcatggctt cggcaagtag
     7861 cttcttgagt ctggtgttct cttcctcaag cgacttcagg cgcttaacct cgggcacctc
     7921 cataccgcca tacttcttac gcctggtgta aaaggtggcg tcggaaatgg cgtgcttacg
     7981 gcagagctca cgggcagaaa ccccggcttc ggcctcgtgg agaatactga tgatctgttc
     8041 gtcggaaaaa cgcttcttca tggggatgtc ctcatgtggc ttatgaagac attactaaca
     8101 tcgcggtgta ttaatcaacg gggagcaggt catttcttac gtgatagcac cttaaattca
     8161 cgccggaaat catcgggagt cgtccttatc tctgggtcaa agtatgaaat atcctcatcg
     8221 tgctgataca tatttacttc agcttcccgg aaccttcggt ctgcattcag taccccagac
     8281 gatttatgaa acaggataat attcatgaca gaagggtctg tgctttcctt tggcttttgc
     8341 gcggaagcga acagtatccc atcgagttcg caatgctcaa atgttgagag ataatcagcg
     8401 atagcttgcg tcagcaggta atttgtttct tcaagctcag gaacaaccgg cataacaagt
     8461 tttctggaga gattctgaat aaaactactg cgttcatatc gaagaagata ctcaggatca
     8521 aagcggctaa atccgttaac agccagccct ttgagactgg agagggcttt gttgaataaa
     8581 tcgaactttt gctgagttga aggatcagat cacgcatcct cccgacaact cagaccattc
     8641 cgtggcaaag caaaagttca gaatcaccaa ctggtccact tacaacaaag ctctcatcaa
     8701 ccgtggctcc ctcactttct ggctggatga tgaggcgatt caggcctggt atgagtcggc
     8761 aacgccttca tcacgaggaa ggccccagcg ctattctgat ctcgccatca ccaccgttct
     8821 ggtgattaaa cgcgtattcc ggctgaccct gcgggctgcg cagggtttta ttgattccat
     8881 ttttgccctg atgaacgttc cgttgcgctg cccggattac accagtgtca gtaagcgggc
     8941 aaagtcggtt aatgtcagtt tcaaaacgtc cacccggggt gaaatcgcac acctggtgat
     9001 tgattccacc gggctgaagg tctttggtga aggcgaatgg aaagtcagaa agcacggcaa
     9061 agagcgccgt cgtatctggc gaaagttgca tcttgctgtt gacagcaaca cacatgaagt
     9121 tgtctgtgca gacctgtcgc tgaataacgt cacggactca gaagccttcc cgggccttat
     9181 ccggcagact cacagaaaaa tcagggcagc cgcggcagac ggggcttacg atacacggct
     9241 ttgtcacgat gaaatgcgcc gcaaaaaaat cagcgcgctt attcctcccc gaaaaggagc
     9301 aggttactgg cccggtgagt acgcagaccg caaccgtgcc gttgctaatc agcggctgag
     9361 cggaagcaat gcacggtgga aatggacaac ggaatataac cgtcgctcga tagcggaaac
     9421 ggcaatgtac agaatgaagc agttgttggg agattcactg acgctgcgtg actacgatgg
     9481 tcaggtagcg gaagctatgg ccatggtgcg tgcgttgaac aggatgacaa aggctgggat
     9541 gccagaaagc gtgcgtattg cctgaaaatc cagccagcta cagggtcgtt cgcacgaaat
     9601 cttatttatt caacaaagcc tgtaacggat catcccggca atcgttacct gtatcgttcc
     9661 tatatcagat tcgaatttcg gcagcaccgg ccagtaagga atttcaaaat ccacactggt
     9721 aagcgcctgt tcgtaaccct gctcatttgt ttctgcaacc gttttaaggc ctggaaaccg
     9781 ggcgtgcata aatttgataa tctttaccac attgggcgtg ctggcctgac gctgatatat
     9841 ttctggcagc catgtggacg tggtggccca tgtcgtggtg catgcataag ttttttgctc
     9901 catatgccat aaaatagagg tgtcagctag gaagtgatga acagtcaaca gctcggccag
     9961 cagccgatct tcatctggat gtgttgtggc atttttgaaa cagacctgta cacggaaacg
    10021 ttcttttgca gacagaaaag tgtttcccct actgacggcc actttgtata acctcctggc
    10081 cggatcagtt tcacaaatcc cggttagaat ccggctcatt ttgactccct gatgacttcc
    10141 accagcccta tacggggcag gacagaacga tatttttcat taaaatcgcc gcgcttctgc
    10201 ctttcacctt ttacgtaatt ttttaacgga ttaaaccggc ccggttcgct gaacaaaatt
    10261 aatggtaaag gcgcgcgaaa gttgagtttg tacggcccat gaacatcatc tggatcttca
    10321 ggactggtgt accggatatt gacgttaaca gccggaaagt tactttcacg gatgagccga
    10381 aattttctca aattttcatc gtcatgactc acttcatcat tgagcaacga cagcatcatg
    10441 atttcttcgg ttcgttcctg tttcagccgt tcaacatcct gttttgtcag actgatatcc
    10501 ggctggagct cagttatctc cctgcgtaag cggtcgataa ccgtttccag ccgggtaaat
    10561 ttatgaatga cagggatttg ccaggtgaac cccagggagc gaatttgctc agtaacgatc
    10621 cggatattac gggacacctg taaagtcacc agccacgggt aaacctgatg cagaatttct
    10681 gaccgggagt cctggtaatg agtgatagct tttacagcat cagcaaattt gtcgcgaagc
    10741 cggttgattt cacccaccag cgacatcacc tgatcatgat tgtttctgac cacaacgaat
    10801 ccgggatatt ttcgggtgat tttggttgaa taatgctcat ccctgacatg gactttatca
    10861 acccgctgct gccggtaaat atccaccagg gtgtcgacgt cgggtaccga atgagtcaca
    10921 ttgatttcct gtggaggatt caactcctca acactacggg gctccgggag cgtggcaagg
    10981 taagatgagc cgatatcgct gtgacggagt aaactgtgca gctcaccgag tttgcgtagt
    11041 tgctcttcgt tgtaatgggc aaagtcagta ttcatatcgt ctcccgtatc gtattcctga
    11101 acgtgcgacg gtaatcgtta aacaaactct ggtaaagact taaacgtcag ccatacaggc
    11161 tggcgtcgcc cgtccctgca tactctgacg aggcgataac ggtttttctc catcgtgacc
    11221 agccagtacc catcggagac agggcgatcg agagtgaaca gttttaaccc gtaaaaaccc
    11281 atcgaggtaa acagacgccc ttcccgctcc cggaaagaga ccgtaaagga cgggaacaga
    11341 agccagaatg cagtgaagaa aacataccag gtatacggaa cagtcagttg tagaatttca
    11401 tagttcctga cggtagctgt gaccaggcca aggctgaata cagcagcaat gctggcatat
    11461 actttgaaag acgcgctatc ggagtttttc caggtaatac cactaataca atgcactttg
    11521 ctcatcgcct tgcctcaatg tagttgtctg cgcactttca tccggggaag aatttctttc
    11581 tcccacagag accttgcatt cccatgcccc cccaggagtt gcattgcaca aaccctgtta
    11641 aacacggtta cttctttcct gattgcctgg gtttggctgt ctgtgagctc aatgcattcc
    11701 ggtaccagct gcataacacc gttcatcatt tcggccttta tgaggcctgg accgtcttta
    11761 gggtcggcct cacaggattc ctttacatta aacccgaatg aacaaagaag ctggccctgt
    11821 gccttaggcc ccatctggcc cccccggcta tcgacggtaa agcggacgat cgcgtaatcc
    11881 cctccctgat cgctggttat gtttcttacc agcatgctga ccggagtcat atgaagccag
    11941 ctgtagatac ccaaaacaat ttcttctttt aaaatcatat tcaattttgg attggcccct
    12001 atatttccat acccttttta tcacttaacc cattactggt tcgccgccgc agatattccc
    12061 gtggcgaacg ataccccagc gcactatgcg gatgccattc gttgtaatgt tcgaaggcct
    12121 ccgcaaggtt ctttaccgct gttaacccgt cgggtttcgg catgatgctg atgtaatcgc
    12181 gcttcatcgt tttcacgaag ctctctgcca taccgttgct ttccgggcta cgtaccgccg
    12241 tatgtttagg ctccagtcct accattctgg cgaactgacg cgtctgataa gaacggtagg
    12301 ctgaaccgtt gtctgtcagc cactcaacgg gggatgtcgg caggctgtta ccgaaacgac
    12361 gctccacggc acccagcatg acgtcctgca cggtttcact gtcatatcca ccgttactgg
    12421 ccgcccagta aagtgcctcg cgatcgcaac agtccagagc gaacgtgacc cgcagttttt
    12481 caccgttatc acagctgaac ccgaagccgt cagagcacca ccgctggtta ctttctccaa
    12541 cggccacttt ccctgtatgc gcccgcttcg agggcggtat ttccggttta cgctcaagca
    12601 gcagcgcatt ctgacgcatg atgcggtata cacgtttggc attgatcacc gccatgtcgt
    12661 cagtttctga ttgtctgcgt agcagtgccc atacccgacg ataaccatag gtgggcagat
    12721 cgccgataac agtatggata cgggccagcg cttcagtatc atcaggcttg cgcttgcgtc
    12781 ggcgatcctg ccagttcttc gacctactga ctatggcatg cagttgcgca cgtgagatcc
    12841 gaaggcaacg actgacgagg cctatttgcc atcctccggc aacaagggca cgtgcgctat
    12901 ccactttttt tgtcggccat attcaacggc ttctttcagc agctcgtttt ccatggtttt
    12961 cttgcccaac aggcgctgca gctctttaat ctgcttcatc gcagatgcca gctccgacgc
    13021 gggtacaacc tgttcaccag cggcaacagc agtgagactg ccttccttgt atcttgaaga
    13081 ctgattaaaa ttaatacgat tagatttggc aaaacgtatg cagcccgatg ccttccaggg
    13141 tacaagcccg tatatcagcg actggcgcat acgccgagcc actcatctga aacccgaaca
    13201 gttgtccgta ttccataacg tatttgcaag gaggggtaaa aatgagtcag ccaaatccac
    13261 tttgtgtggg catcgatgtt tccaaagcca cactggacat cgctgccagc agtgatgttg
    13321 ctcagtttac agtcagcaat gactctgatg gctttaacgc gattattacc ggactgagac
    13381 ggcactccgt tgctctggtt ctgatggagg ccactggtgg gctggaagtt gcggtggcct
    13441 gttcgcttca ggccgaaggt tttgaggttg ccgtggtcaa tcccaggcag gcccgtgact
    13501 tcgcccgtgc aatgggatat cttgcgaaga ccgaccgaat tgatgccaga gtcctcacgc
    13561 agatggctga ggttattaat cgtcaccctg agcggaaacg ctttatcagg gcactgccgg
    13621 atgctgaacg tcaggccctt gctgcgatgg tggttcgcag acgccagttg attgcgatgt
    13681 tggtggctga gcgtaaccga ctgcatccat tccatccgca gaacaagaaa agcattaata
    13741 ccatcattaa agcgctggaa gatgagcttg tcaggctcga gaaagaaatg gacagtcaca
    13801 tccgtaacca ctttaaagag attgctgagc gcctgagcag tatcaaaggt gtaggcacca
    13861 tgaccgtttc cgcgatgttg gcggagattc cggaactggg aacgctctcc cgacgcgaaa
    13921 tcagtgcgct tataggtgtt gctcctgtca accgggactc agggactatg cgtggccggc
    13981 gaaccatttt tggcggcagg gctggcgtaa gatcagcact gtacatggct gcgcttgtgg
    14041 caacccgctt taatccggtg ataaaaacgt tttatgtgcg cctgcttgca gccggaaagg
    14101 ccaaaaaagt ggcgctggta gcctgtatgc gtaagctgct taccatcctg aacgcaatgc
    14161 tcagaaagaa cgaagaatgg gatgagtcgt accaccacgt tgctccataa tttttacctt
    14221 caagacagtt gctgatattg cttacgccac aggaacagct gactggcagc aacaccatgc
    14281 tgacgggcga ccagcgacac ggtcattccg ggttcaaagc tctgctgaac aatggcgatt
    14341 ttttcctgaa cacttcgccg cctgcgcttc tctggaccta aaacatcaat catccggact
    14401 ccaacgacta gtctaaaaac tagtattaag actatcacta acttaagtgt aagcgttaag
    14461 tgagcgccgt attgacggtt atttatcggt aagaaccacg ttccatggca gcagttcggc
    14521 cactttgttg ctgggccact ccggcagtac gctcaggata tggcgaaggt aggcttccgg
    14581 atcgatacca ttcagcctgc acgtcccgat cagtccgtac agcagggctc cgcgctcacc
    14641 accgtggtcg ctgccgaaga agataaaatt ctttttccca agacagacgg cacgaagggc
    14701 tcgttcggca gcgttgttat caggctctgc caggccatct tcacagtagt agcacagcgc
    14761 atcccactgg ttcaggacat aagcgaacgc ttcgcccaga cgggactttt tcgacagcgt
    14821 ctcatttttt tctaccagcc attcatgcag cgatgccagc agtggtttgc tttgcgattg
    14881 tctggctgcc agacgctctt ctgtcgtgag gccgcgtatt tcttcttcta tcgcgtacag
    14941 ctcaccgata cgtttcaggg cttcttctgc tgtcgctgtg tgagtgctta tatagacatc
    15001 atggattttt cggcgggcat gggcccaaca tgcggcttct gtcagcggcc ctccttcacg
    15061 ttccggactg aacaaccgat cgtagcctgc gaaggcatcc gcctgcagca caccatgata
    15121 gtggcgaagg tgttgctgag gatgttttcc ctgccggtct ggcgagaagg cgaaccatgc
    15181 cgctggcgga tctgatgaac ctgcgcttct gtcgtcacgt acatacgtcc agatacgccc
    15241 ggttttcgtc ttctttctgc ccggtgcaag caccggaacc ggagtgtcat ccgtatgcag
    15301 tttgtggcag tccatcacgt agtggtaaag ggcctcatcc agcggggcca ttaaccggca
    15361 acaggcatcc acccagttgg agagcagagc ccggctcaga tccacgccct gacgggcaaa
    15421 gatttcgcac tggcgataca gcggcaggtg ttcgcagtat ttggccgtta acacgcgggc
    15481 cagcagaccc ggcccggcga taccccgatc gatgggacgt gatggcgcag gtgcttcaac
    15541 gatacagtca catcgggtgc aggccttttt caccctgacc gtgcggatca ctttcagggc
    15601 gctggagacc agttccagct gctccacgct gacttcgctg aggtacgcca taccgctgcc
    15661 gcattccgga cagctggttt ccgcaggatc cagccgatgt atctcgcggg gaaggtgtgc
    15721 cggtaacggg cgacgatggc gggactgacg taactggcga ggtacctgcg gatcgtcttc
    15781 ccgtccgttg tagcgatcgc tttgctgctc ctgctgtttc agcagggctt cggcttcttc
    15841 aacctgtcgg cgaagttttt cggagcgggt accgaacaac attcgacgca gtttttctat
    15901 ctgtgcccgc agatgctcta tctcgcgctc ttcgttctct attttctctt ctgcgcgggc
    15961 cagtgcagaa cgcaggaacg actcagtctc tgcaacgaga ctgagctgac tgtctttctg
    16021 gcgaagcgcg tcttccagcg cagcgatacg ggcgagatag tcgtgattca taaccgcttt
    16081 tataatgcgg ttatgatttt tttacaacat tgtcagtgag ttaaggcggg atatttttgg
    16141 ctggcgccag tccagcttat caaggagcat ggcaagttgt gagcgggtga tcgcgatttt
    16201 gccgtcgcgt acagcaggcc agacgaactg tccctcttcc aggcgtttga taaacaggca
    16261 cagaccatca gcatcagccc agagtatttt cacggtatcg ccacggcgtc cccggaagat
    16321 aaacagatgg ccggagaagg gatcctcatc cagaacgtgg tgtatctgtt cgcccagccc
    16381 gttgaaggat ttacgcatat ccgtggcccc ggcgaccagc cagatgcggg tgcctgacgg
    16441 gagggatatc attgtgtcct cccggtcagt tcgcggatca gcacagtcag caactctgac
    16501 gacgggttct ccagcgtcat tttgccgtgg cggaactcaa catgacagga agtggcgctg
    16561 gcgtgagcgg aggagaacgt acacagagtg tcattctctg ctgcgggcaa cgggtcgttg
    16621 ataacgtcga cggacaacag aatcggagaa gccggaagag attgaggtac caccggcccc
    16681 acgatagttg caggcattcg acgagagatt cgcccctctc tctgccagag gcgtagccac
    16741 ttaaaaatga gattattatc aacgccatgt tcccgcgcca gttgtgcgac gttggcatct
    16801 ggtcgtgaag ccagttcgac catccgaagt ttgaagtcac tggaatagac ggtacgcgat
    16861 tcagttcgcc agttggcagc attttccatg aacagaactc cttctaaatt agacggagct
    16921 ctaagctagc gatcccggga ctggaatcac aggcggcagt gacttgacgc ttacgacata
    16981 tttccgtttc catcaaaacg tctccgttcg ccgttaaaga gccttactac ggcaacgtgg
    17041 ccacggagaa actgcttaca cccactcagg acaccaggga catcattgct gacgccacgg
    17101 cctcacagtg ctgagctgat gatggttctg gacgggatca acaactcagg gctgggtaaa
    17161 gtatggtttg ccggtagagg aattgccccg gaatggccga tgaaacgtga gatgccttca
    17221 tcggcatata ccacccgctg gaaggagctg ccggttgccc gtttctgacc ggcgaaatga
    17281 cagacggaac ttgctgccag atcatacttt accgatcatg cagatgactt gtctcagggg
    17341 ggaaccatga caaatatcat tctgtgtgat gtaactgcca gcgtcagcga acttaaaaac
    17401 gatccggtgg ccagcgccgg tgccggctgc ggctacccgg tcgctattat tgacagcaac
    17461 cggcctgttt ttttattgtg tgcccgcggt tctgtaggaa aaaatgctcg atcttctgga
    17521 cgcaaaagac ctggcgcaat tgataacaga acgtcaaaac caatatattt tgtgatcgga
    17581 caacgtgaca aacgacatca cagtggagac actgctgacg aggtgatccc aaggtcgtaa
    17641 aacggccgga actggttacc ggccgaacga aggaccggcc tacgcgcagc ggcgacaact
    17701 ggctttaaaa atgctggcgg aaatttaaaa ttaagccgca taccctgcga aattttgcag
    17761 atttcactca acatgcatgc ttcgttcatt tcccaacaga gctctcacca tcgcctgtaa
    17821 tagcagtcct gctgcgtgat tactccgctc aacgggtcac cggcgccatg ccaatacctg
    17881 ttcgtgacag gcattgatgg aactgcgatc ccaaactaat aatgtaacga cgatgcaggt
    17941 gggaccgtgg tcccaggctg aaaacctaaa cgacgataca ggtgggaccg tggtcccagg
    18001 ctgaaaacct aaacgacgat acaggtggga ccgtggtccc aggctggaag cctaaacgac
    18061 gatgcaggtg ggaccgtggt cccaggctga aaacctaaac gacgatacag gtgggaccgt
    18121 ggtcccaggc tgaaacctaa acgacgatgc aggtgggacc gtggtcccag gctggaaacc
    18181 taaacgacga tgcaggtggg accgtggtcc caggctggaa gcctaaacga cgatgcaggt
    18241 gggaccgtgg tcccaggctg ggagcctaaa cgacgatgca ggtgggaccg tggtcccagg
    18301 ctgggagcct aagcggcgat acaggtggga ccgtggtccc aggctggaag cctaaacgac
    18361 gatgcaggtg ggaccgtggc cccaggctga aaacctaaac gacgatgcag gtgggaccgt
    18421 ggtcccatag agagtattca ggccagttat tttttctggc ctgtgagaaa ggaaatgaag
    18481 taaagggaga taaccgtaga ctaaagcgtg tccgcatcaa actcctagtt tctcaagctc
    18541 cttaagaatg gcttctattt tctctataca ctccgcagga atgcgggacc tatccaggtt
    18601 aagcaccatt ttatcgccct tgtacaatgc tgttgcccct ggagcaaact gatgacgtga
    18661 gctcagatta actcttgatg cggaagattg tttcagtacg gatgttaaaa gactaatgac
    18721 ctcttcagct tcgaagatta gtcccgcttt cttctgatcg tggagagtct cagcctgctg
    18781 cttcagtaat tcttctttat ctgcaaaagc cttctgaagg gcttcacctg accgggcaga
    18841 cagttctcca ggatgtgcaa acagggcaac aacggattta ggcagtttgg ccgtattgat
    18901 acagcgggta atgatcttac gagagatgtt ttccgcatcg gcaagtgcag aaatatttcc
    18961 agcaaactca ttctgcagcc ggcttgtata acgcagacca cgttcataag cactcgtcgg
    19021 ccgataatcg ttacccagtc gggacaatgc tgccatctgc tcatcgtcca gttcaccaac
    19081 cagaatgcga taatcacttt ccgtcaggat tgcggcttta cggcgacggc tgccgtctgc
    19141 aatttcaatg acatcggaaa cccggcggcc gaaagctgga gtctgctgac cagtcagtaa
    19201 aaatgaagga atgagatcat ccagagcatc ttcggtaagt agttcctgat cgcgctcatt
    19261 gcctgaccat acgcgtgaag cgctctcaac agagtctccc cggagcactt caagggtaaa
    19321 ttttacttcc cgtccacata ccggaagaga gatggcatta ccgcgggcca tagcacccac
    19381 gcgcgcaatt aatgaatcca ccataggcgc agccggcgcc ggtgatgagg tatcttcagt
    19441 cgattgagta tgtgtggtat gtcttggaat gacaggggca cgcttcatta tctaatctcc
    19501 cagcgtggtt taatcagacg atcaaaaatc tcatcgcaaa caggctccca gatagagagg
    19561 gcattacgcc aggcacctgt tgaagaacgt tgatcaatag cctgttcaaa aacggttctc
    19621 atacggatct gacctttacc gacttcatcc gtttcgcgca caacattttt aaggaccatg
    19681 cttccccagg catcccgaat ttgttcttcc atccacgggg actgcgagcc gttattatta
    19741 ctgtatttgg taagcagaat acggacatct ggctcgaagc ctttaagatc cacgttctta
    19801 agcaggtcac gaagcatatc gaaaaactgc agtgcggagg tgtagtcgaa cagctctgcc
    19861 ggagtaggga caatcaatac atcagcagcg catacaacat ttatcgtacc gataccaagg
    19921 ttgggtgcac tgtcgataac aatcacgtca taatcatgag cgaccgtttc aatggccaga
    19981 cggagcatca ggtgcgggtc agtaggtaat ttgccttcgt caaatttccc catcagctca
    20041 gtttcaatgc ggtgcagtgc cagacaggac gggatgatat caaggccagg ccagcaggtg
    20101 ggcttaatag cgtagctggc atcatccttt tccccaagat aaaagggcag gagagtgtcc
    20161 tcagcatgaa tatgaagatc gggcacccat ccgtgataca tagaagccgt tccctgtggg
    20221 tcattgcctt caacgagaag aacacggagt cctttcaggg ctaggtcctg agctaggtga
    20281 acagaaacgg aggttttgta aaccccccct ttatgagccg caaccccgat caccggaggg
    20341 aaagcatctt ctgcacgccg cagtcgggta ccgaacacat cccgcatatg attgatttgt
    20401 tcgatggtat aaccaacacg ttgttcaact ctgccacggg tttccatatc agggtgaggt
    20461 aaccggccgg ctttctctgc atccctgatt gcctgagaag aaacgccaac cagatccgcc
    20521 gcttcaccaa ttctccagcg gcgggtgatc ttccttgctt ccggactgtc gtcattaaac
    20581 tgcgcaatgg cgatagcctt cgtcatctca tgtccagcag taatgcactg gttaagtata
    20641 tccatcagtc ccattctgag catcctttaa tcatcgcttt gcatcatttt attaaatctt
    20701 gggattattt gcaaagcaac aataaaatcg caaagtcatc tgaaaattgc aaagctgact
    20761 gtaatgaaag ctgtaaaccg gagaagagag gctcaatcac ataccgcagt caatatttct
    20821 cactaccgct cgccgcagta agcgatctca cgaagtgagg aagtggaaaa gcacagagac
    20881 tgcacaaagc acgaacgcta cgatgccgga tacttctccg gattagtcta tcttcatgaa
    20941 attgactgtg atatgtgaaa aattgactat ggtatgaaac taaaagccga gggctggtca
    21001 acaacaaaag aagattctac atatcgcagt caatattcaa tgttaaatca gggagagtta
    21061 ctcatgcggc attcagatta cccttgcaga accccgaatg tggtggggta gatctggctg
    21121 gggattaacg ttattcattg cttcgatcca gtaattgatc ccaaagagat catatcgaga
    21181 tcctaaagag atccccggat cgttgcagca ggcgctataa ctggcttgag cgtcatactg
    21241 gatcgactgt ggaatgtaaa aagttgactg ctgtatgcga aatctaatac tgtgatatgc
    21301 aacggtattt actgaggtat gtatatccgt tgactgcgat acgtataacg acattctttt
    21361 gtggataacc gttgaggatc aag
//
