LOCUS       NZ_CP070601             5598 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KPA9853 plasmid pA9853_1, complete
            sequence.
ACCESSION   NZ_CP070601
VERSION     NZ_CP070601.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933305
            Assembly: GCF_016939835.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939835.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 04:48:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,392
            CDSs (total)                      :: 5,271
            Genes (coding)                    :: 5,146
            CDSs (with protein)               :: 5,146
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 56 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 28 of 125
            Pseudo Genes (multiple problems)  :: 13 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070601.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPA9853"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pA9853_1"
                     /geo_loc_name="Czech Republic: Mlnk"
                     /lat_lon="50.34 N 14.48 E"
                     /collection_date="2019-10-08"
                     /collected_by="Nemocnice Mlnk"
     gene            join(5420..5598,1..172)
                     /locus_tag="JW307_RS01005"
                     /old_locus_tag="JW307_01005"
     CDS             join(5420..5598,1..172)
                     /locus_tag="JW307_RS01005"
                     /old_locus_tag="JW307_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(242..508)
                     /locus_tag="JW307_RS01010"
                     /old_locus_tag="JW307_01010"
     CDS             complement(242..508)
                     /locus_tag="JW307_RS01010"
                     /old_locus_tag="JW307_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(492..716)
                     /locus_tag="JW307_RS01015"
                     /old_locus_tag="JW307_01015"
     CDS             complement(492..716)
                     /locus_tag="JW307_RS01015"
                     /old_locus_tag="JW307_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            879..1007
                     /locus_tag="JW307_RS27080"
     CDS             879..1007
                     /locus_tag="JW307_RS27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(1383..1595)
                     /locus_tag="JW307_RS01020"
                     /old_locus_tag="JW307_01020"
     CDS             complement(1383..1595)
                     /locus_tag="JW307_RS01020"
                     /old_locus_tag="JW307_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1602..2111)
                     /locus_tag="JW307_RS01025"
                     /old_locus_tag="JW307_01025"
     CDS             complement(1602..2111)
                     /locus_tag="JW307_RS01025"
                     /old_locus_tag="JW307_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2146..2850)
                     /locus_tag="JW307_RS27175"
                     /old_locus_tag="JW307_01030"
                     /pseudo=""
     CDS             complement(<2146..2850)
                     /locus_tag="JW307_RS27175"
                     /old_locus_tag="JW307_01030"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2789..3133)
                     /locus_tag="JW307_RS01035"
                     /old_locus_tag="JW307_01035"
     CDS             complement(2789..3133)
                     /locus_tag="JW307_RS01035"
                     /old_locus_tag="JW307_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004197764.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3123..3299)
                     /locus_tag="JW307_RS01040"
                     /old_locus_tag="JW307_01040"
     CDS             complement(3123..3299)
                     /locus_tag="JW307_RS01040"
                     /old_locus_tag="JW307_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165764108.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSV
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            3939..4097
                     /locus_tag="JW307_RS27180"
     CDS             3939..4097
                     /locus_tag="JW307_RS27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4457..4783
                     /locus_tag="JW307_RS01045"
                     /old_locus_tag="JW307_01045"
     CDS             4457..4783
                     /locus_tag="JW307_RS01045"
                     /old_locus_tag="JW307_01045"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197760.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINEMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            4809..5354
                     /locus_tag="JW307_RS01050"
                     /old_locus_tag="JW307_01050"
     CDS             4809..5354
                     /locus_tag="JW307_RS01050"
                     /old_locus_tag="JW307_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433973.1"
                     /translation="MFFKRYLTEKEIYKVALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     Typing          complement(3386..3466)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.476"
                     /coverage="98.0"
                     /evalue="3.1e-22"
     Typing          3651..4048
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.801"
                     /coverage="91.0"
                     /evalue="6.52e-141"
     Typing          complement(1268..2799)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 caaagaaaag ggaatcgaag tagtaatgca gagagctgct tctgatgata actctgaggc
       61 cataatgtct tttattatgc ctgacgttaa caatatgacc ttattagtta aaaataacgg
      121 ctacattatc gttaagacaa cctgctggtt aactaaaacc gacaaacagt agaacacgta
      181 gttagtcgag tatttaccat aatacctgtt gtcggcatca aaaaagcccg catagcgggc
      241 ttcagtcgta aacttcgcgg cgatgtccaa tcgtggcggc aaggataaca agctcgtcat
      301 ctcggatctc acacagaacg cgataatctc ccacccgata gcgccagaac tcgcccagct
      361 cacctttgag cggcttccct gactggcgag gatcggcagc aactgcaatg cgtaaactca
      421 taaaatccac aatccgtcgt gcgttctgtt tgtccatctt gcgtaacgat ttgagcgccc
      481 gatcggaata gttaatcgtc cagaccaagt gacttcctca cgtcttcaga cgaatgcact
      541 gcttcatcac cacggcgaac gcgtgcagca gtttctgctg aaagataata atcctccagg
      601 tcttccagat gcgccagtat tgcttcccgc gcataaaacg tctttgttct gccggtttgc
      661 ttcgccagcg agtccagacg ggactcaatc tcatcagata accggatagc aagcatataa
      721 acctcccgaa caccatttgc tatacatgta tatcaacagt accacaatca acctaaaaga
      781 acaaaaaata atcttgttag cccctcttga taatccgtaa cgcggtcaat atatctagct
      841 tgagggcttt cctcttaact attgaaagga taacgattat gggtcttaag ccgggtccga
      901 aacgaattgc cgattcaaca ggggaacctg ataaacgcca gcgtgacaat aaaaaaacgc
      961 cgggaaatac tgacaaattg aaacccagca aatcttcaaa aaaataagtc agtagttaaa
     1021 agccctccgg ggcttttttg tatctgccgt tcacgctgat taaacataac acccgttgcc
     1081 cgcaccgccc cggacgggtt cgccgcgaac ggggtggcag tcaggtaaaa ccggcaacaa
     1141 accacccgat aaaccgcccc gttcgcgaca gataaatttt acctggctgc atagccatgc
     1201 agccaggtga atgacatccg gtttcagcgt aaatgcccga tgagaaccct gttttcctgc
     1261 atttcagccc agcgacggac cgtcatagct tcgctgccgg gagcgcgctt ccgtcagttc
     1321 cttttcgtgc tgccgttgct cgtgcgcccg ttctgcggcc acggccatct catgacgctg
     1381 cactaccggc tcaaacgctg cagttgcccg ctcaagcgtt ccacctgctg actcaagcgc
     1441 gtcacgcgct cgctcagcgc cgcgttctcc cgttgcataa gccctgacat tttccgccat
     1501 tccccgaagg cgctctccca ctcgtccagc cttttcgagt agtcctgctg tagctgctct
     1561 aatgcgctca gcaactgttt ttccagctcg gtcatcggca cttactcctt caccagtctc
     1621 agcgcgttct gcttcgaccg cccgttcccg accgtccagc tctgcgtcct gtctacggcc
     1681 gtccccttcg gcagcaccag aaaacgtccg ttgctgtctt ccaggtacgt cacgccccag
     1741 gtcttcgcgt tcagcttcga cagcgccgcg ttctgccggt cgatttcggc cagatttgac
     1801 gctatcaggc tcccctgata ccacaacacc ccgctcaggc tggcgaacag cagtcctgac
     1861 accatcgtga tggtcagcca tgtccgcccg accatacgca tcacgctcag gcggttcgac
     1921 tgcatggccg cgctcatacc ctgctcgtgg gcgcggatgg cggcgctgat tctcttcccg
     1981 ctttcgctca gttccgctct gacagacttc tcgtgctcgc tgaatgcggc tttcagcatt
     2041 tcgccggtac tctgctgctg cgctttcgat ttctgctcta agtccttcgc cagcgttaaa
     2101 agactgttca tagatggctc ccttaagtcg gatgtttcgc cctccgtccg ggtcggcaat
     2161 gctgatgctg ctctttgtgg tgcgcacgac ctcaaaacct gcgctttcca gcgcctcagt
     2221 gacgtcctga cgcgttttaa gctcccctga cgaggcgagg gccagtaagc cccgcgtaat
     2281 agcctctgcg gcttcctgct tcgttttggg taacgaagat ggcgtcacca gcgcgcgccg
     2341 gttctccggt gcgttcgggt cgtgcagccc cagtcgcccg ttcaccacgg tctgccacgc
     2401 atcgatgcgc ggacggtctg cccggtcgta gtacggctgg agacgcctgc cggtcagcag
     2461 ttccgtgttc ggtaccagaa aattcagttc cagccgcccc ttgtcccggt gttcaaccca
     2521 cagcacgctg tactggtctt tatcgagtcc gggcatcaga acccgctcga agctcgccat
     2581 cagcttttca cgctggccgg gcggtaaatc ctgttcagca aaagacagga cgccggaggt
     2641 gtactttttg gcgtagggcg aggcgtcgat aagctcccgg acctcctccg gctttccctg
     2701 caggacgatg gccccgtcgc gctggcggtc tttccccagc agataatcca ccggcccggc
     2761 accgccgcct cgccctcgcg gatgaaactt aacgatcatc gtctgccccc ttttccagca
     2821 cggcatgccg cagccgctcg agtccggcat cgatggccat cagcgcggcc accacctgaa
     2881 cccggtcagc gcctgaccac tggccgccgt taattttccg ggcgatctgg ttgaggttgt
     2941 tccccatccc ggcaagctgg cgcagcaaaa ccggatcgat ggacggcagc cgcaacgacc
     3001 gcgccgggcg cgtatcgaga caggtttccc gcatccacac cgccagttgc cttccattgc
     3061 agcgctccag cagctgctgg tactccccat cggtcacccg gatacctaaa aaacgggtac
     3121 gcttaaccgc catcctctcc tccgatatcc gcaaaatgtg accggactga acgctcaagc
     3181 tctgccatct gctcccggcg gtaatcttcc tcctgtgcct gacgcatcag atttgccatc
     3241 actgccggat gccaggatct tttttttacg acgagcggct tcttcttcgg cttctgcatt
     3301 atccctcctt cgcaaacgcc ggacggcacg gaaatgtttt ccgggacgcg ccggcgttgg
     3361 acaagcgcca gcgcgtcagt ggtgttagcg ggtttcgggg cgcagccctg aaccagtcac
     3421 agcgtttacg ctgtgtatac tggcttacta tgttggcatg agagcgagat tcatgaaagt
     3481 gcctcatgtg gcaggtgtga tatgtgactc aggagcgtcc gcaataccat gcacggacag
     3541 atgaaatgac gcctcctcgc tcactcggtc gctgcgctcc tgccgttcgg ctgcggcggg
     3601 cgtgaccggc tcacggataa cgggataaag caaaggcgct caaatcagaa agtgccggag
     3661 aaaaggatac ggcgtggccg tttttccata ggctccgcct cccctgacga gcatcacaga
     3721 aatggacgct caagtcagag gaggacgaaa cccgacagga cttaaagatg ccggtgattt
     3781 ctctccggcg gctccctcgt gcgctctcct gttccgaccc tgccgcttac cgaatacctg
     3841 ttccgccttt ttccctttcg ggaagcgtgg cgctttctca tagctcacgc tgctggtatc
     3901 tcggctcggt ttaggtcgtt cgctccaagc tgggctgtat gcacgaaccc cccgttcagc
     3961 ccgactgctg cgccctttcc ggtaactatc atcttgagtc caacccggac ggacacgcaa
     4021 aaacgccact ggcagcagcc attggtaact ggatatatac gagagagata ttgagaggtc
     4081 ttgaagtggg tgcctaactg cggctacact gaaagaacat ttttggtatc tgcggtctcg
     4141 tatacccagt tacccggtta agcagttccc caactgactt aaccttcgat caaaccgcct
     4201 ccccaggcgg ttttttcgtt tacagtgcaa gagattacgc gcagaaaaaa aggatctcaa
     4261 gaagatcctt taaatgatcg tcagttctgt tatccccgga gctccatcca tcccacggaa
     4321 aaacacccgc cactgatgcc ctcagcgcac tgcacatcat gtacaaacca ctccctccct
     4381 ccttttttat tgcgtttctt aatgcgcaat ggtagctttg tgcgcaacaa aaaacgcaat
     4441 agcgaggggg ggggtaatga gagaaagaac aaatatcatg ctagatatag gattaaagca
     4501 atccttacaa cggctttcga ataaaaccaa aatgagtcta agtgatctta ttaaccagag
     4561 attatcaggt tcactttcag ttgaagaacg cattggcgaa aagcctgaat ggatgagaaa
     4621 ccttgatccc tatctcgcca atcacttttc aaatattgag ttaccgctcc cttcaccatt
     4681 aaaagaatac caagaaaaaa tggatgagat agattcaatt attaatgaaa tgatggttct
     4741 aaaggaatat ttcagagcta agttttacga cgaaataaaa taggcacgct atacggaggc
     4801 ggagaattat gttttttaaa cggtatttaa cggagaagga aatatacaag gtagcactcc
     4861 caattgttag gcttcaaggg agagttaaca cttcattacg ttttttatcc aaatactcag
     4921 atgtaaacaa aaatggatta attgcattac atcttaattt attacatttt atctcataca
     4981 acatacacgg tgtagagtct ctgaaaatca ctcaagcagg cataaacaaa gctgtacaac
     5041 aaagccctca cgcagatgaa ataaaaaaca ttgcgcaggc aataggacgc aaaggacctg
     5101 agtacatcga aggattaatt agaaacccat gggggaagaa aaatgataat ccactaatat
     5161 ttataatgag cattgaagaa agtataaaag aaacccttaa ctcaggttat atgttatata
     5221 gagtagctcc cataactgag ggtgaagcag ctgctttcat ggatgttctt aaacaattat
     5281 tattatttga ggataatttt cgcctttttg gtagggactt ggtttataaa atagaaaaat
     5341 taaaaatcag ataacaaagt atggtgaacg aaaaatgaaa gagaggatag tgaaatgtgt
     5401 aataccaaga ttctcaacta tgtattggtt aattgcgttc ctagcattta tattccccgg
     5461 tcgatctttt gctgacggtt acaccgctta ctaccgatgt aatggtactc aggaagcatc
     5521 tcttactgtt aaagataaca aactaagtat ggttgtaggt ctttctaagt ttaataggca
     5581 actggatact tatagatt
//
