LOCUS       NZ_CP070597             6233 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KPA4411 plasmid pA4411_1, complete
            sequence.
ACCESSION   NZ_CP070597
VERSION     NZ_CP070597.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933304
            Assembly: GCF_016939815.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6233)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6233)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939815.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 04:54:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,666
            CDSs (total)                      :: 5,542
            Genes (coding)                    :: 5,405
            CDSs (with protein)               :: 5,405
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 60 of 137
            Pseudo Genes (incomplete)         :: 81 of 137
            Pseudo Genes (internal stop)      :: 13 of 137
            Pseudo Genes (multiple problems)  :: 15 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070597.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6233
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPA4411"
                     /isolation_source="endotracheal cannula"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pA4411_1"
                     /geo_loc_name="Czech Republic: Mstec Krlov"
                     /lat_lon="50.20 N 15.28 E"
                     /collection_date="2019-06-17"
                     /collected_by="Mstsk nemocnice Mstec Krlov"
     gene            complement(<5..124)
                     /locus_tag="JW306_RS28225"
                     /pseudo=""
     CDS             complement(<5..124)
                     /locus_tag="JW306_RS28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /GO_function="GO:0050661 - NADP binding [Evidence IEA]"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /name="NAD(P)-binding domain-containing protein"
     gene            complement(288..>1417)
                     /locus_tag="JW306_RS02150"
                     /old_locus_tag="JW306_02150"
                     /pseudo=""
     CDS             complement(288..>1417)
                     /locus_tag="JW306_RS02150"
                     /old_locus_tag="JW306_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529410.1"
                     /GO_function="GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /name="undecaprenyl-phosphate glucose phosphotransferase"
     gene            complement(1430..2127)
                     /locus_tag="JW306_RS02155"
                     /old_locus_tag="JW306_02155"
     CDS             complement(join(1430..1878,1878..2127))
                     /locus_tag="JW306_RS02155"
                     /old_locus_tag="JW306_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="WP_095033700.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGRLMSLLSPFDVVIW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1B family transposase"
     gene            complement(<2186..2458)
                     /locus_tag="JW306_RS02160"
                     /old_locus_tag="JW306_02160"
                     /pseudo=""
     CDS             complement(<2186..2458)
                     /locus_tag="JW306_RS02160"
                     /old_locus_tag="JW306_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012541089.1"
                     /note="WcaJ; glucose-1-phosphate transferase responsible
                     for the addition of the first glucose-1-phosphate from
                     UDP-glucose to the lipid carrier undecaprenyl phosphate in
                     the biosynthesis of colanic acid; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /name="undecaprenyl-phosphate glucose phosphotransferase"
     gene            complement(2536..3108)
                     /locus_tag="JW306_RS02165"
                     /old_locus_tag="JW306_02165"
     CDS             complement(2536..3108)
                     /locus_tag="JW306_RS02165"
                     /old_locus_tag="JW306_02165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899424.1"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]; GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_223367798.1"
                     /translation="MFRLVYKKNILTSPKVKIKNIKNIKFHKNSNLIVGLSNVNHVNNN
                     SICYINNRGEMHVSGNVFIAKSVRVDIADSSKIILNDCYIGPETDLISYSGISIGKGSM
                     VSWRVQFLDEDFHLVSYNDKKPKDGKITIGENCLIGNNVAINKGCIIADGCVVASHSVV
                     NGVFLEKNCLIAGVPARVIKRNISWQH"
                     /name="acyltransferase"
     gene            complement(3229..4968)
                     /locus_tag="JW306_RS02170"
                     /old_locus_tag="JW306_02170"
     CDS             complement(3229..4968)
                     /locus_tag="JW306_RS02170"
                     /old_locus_tag="JW306_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004180511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004180511.1"
                     /translation="MKLLMSKRYFLKKLIPSLTLFPVLTSYASSNKDVIESPQSKRYTI
                     ESIKQYISLFIGECVFISGQEATQDDSFFYSGYFVSIPVIADELIDNVIYIRGKNCTWK
                     RKIDDFIDVSWFGAIGDGINDDSNAISRANIAAHNECLPLKFIPGHIYQVKKTYEIDVS
                     KTSWFSSDLSTLKWFNDFNADFAIRLFSSQKDYSKRFQNVKVAIKSIAIIGAGIKNLLD
                     SCAIKIGGDERNSSLFTIDSVSIQGWRTTLAFDNNSWRIKFCDCHFLWGNIIAPPGNKN
                     SGECMVFDNCMFADNRSYTELHYGDWFFSKCSFDNHEVKLFGDANVFINQSHMENPGRK
                     TTDFTIVSINSINSFASVIDSFIFISPTPKIINTPLFYVISDNENGLYVRNLRFQATEN
                     YNPSKGTENALVLVGGDGKSYLENVRVSLNNKSYLALNKNDSSVLMNSRFKDGLRYWDF
                     NDGVSLQARISSNDSETIVFSKNGASLSQSVLVKSTGILSGGMMLKIVSGDLKLTLECY
                     DSLDNNITTREWNCSASDYSDWSWVRFGEKLPDNIRKIKFYCKSFGQIVDVKLSTILMD
                     IIS"
                     /name="hypothetical protein"
     gene            complement(4999..5580)
                     /locus_tag="JW306_RS28230"
     CDS             complement(4999..5580)
                     /locus_tag="JW306_RS28230"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="WP_266097466.1"
                     /translation="MISYINFPALLGLCIIAKDFVYLVFGEKWLFIVPTLQLLCIAGAM
                     RMVANPIGSLLMAKAKMELSVRFNFVKIIIFIPVLYFSTIWNGMVGAAIGFLICQAINA
                     LLSYFFLLKPVLGNCIVDYINSFFIPFVHTLPMILLLLVCDIYIDKISLTFFILKIVIG
                     GGVYILTIFISPNKLLAEMKGLFRQLLLKR"
                     /name="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
ORIGIN
        1 ataattcttc ggtcttttca cgggagcggt tgaaaacgga gacgtataac cacggctttc
       61 gatgtcagcg caaggttacg cccatcacag ccataccgac aaccccgatc tgttgcttgg
      121 acattactta ctcctgtctg gtgtggtcac tgcgacacgt tcgcagctta aatgtgatga
      181 taatgttagc ccaaagtaca aagagctcat agtgaaaatt tgttaactag cacaagaaat
      241 aaattttgga ctataaatgc acaaaccttg tcttaaggag catctaatca ataagcagat
      301 ttgttaataa atcctttaaa gacagtaagg aatattattt ttagatctaa ccaaatgctc
      361 caacctctga tataacaaat catattcgat tcgtttttcc atttttcaaa gtatctgttt
      421 caccacgcca accattaatt tgtgctaaga ccgtgatacc tggcttaacc ttatgacgaa
      481 gcatatatcc ctgaattaag gaccgatatt gctcgttatg agaaactgca tgcggtcgag
      541 gtcctacaac ggacatctga ccaaaaagta cgttaaagaa ctgcggaagt tcgtctaagg
      601 aagtactgcg taaaaacttg ccaactttag tcacgcggat atcatttttt gtagcctgaa
      661 taactttatc gtcgttttcc attacagtca tcgagcgaaa tttccatact tttataggtt
      721 ttccatccat tccataacgc acctggcgga aaataaccgg ccctttcgag gtagttttaa
      781 ctgcagtagc aataaccaga agaattggtg atatcagaat aagaatcaat gatgatacaa
      841 tgatatcttc aaggcgctta aaaaccatgt taatgccatt cagcggggaa tcaaataatg
      901 gaacaaccgg tacgccattt atttcttccg tacgcgactg cagtatatta aaagtaaata
      961 catcaggtat ataaagaaca gaacaagtag tatctgttaa ttgtgaaacc atatttttta
     1021 tagcaacctc atctctcata tgcattgcaa tgtaaatacg gtcaatagtt ccttttcttg
     1081 cctcatttat taaatcagaa atttttccag cataaagttc taaatcataa gtacctgaaa
     1141 aggagtcttc atatatacct ttaacctcaa accctaacca aggctgcttt ctaaaagttt
     1201 ccagcaaact gattcctgct ggtaaagacc cagcaaaagc aactcttctt ttattataac
     1261 caatccgtct aaaaaatcct gctaatgctc tgatcaaaga acgagtaaca acaaagccag
     1321 cacatacagc caaataccaa aaaataaatg ttctaaatgt taaatcaaaa tctgaaaata
     1381 acgtaacaaa accgagcgtt agtaaaaaag aaagggtaat gactccaact tattgatagt
     1441 gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat tttgagaacg
     1501 acagcgactt ccgtcccagc cgtgccaggt gctgcctcag attcaggtta tgccgctcaa
     1561 ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc cttcaggcgg gattcataca
     1621 gcggccagcc atccgtcatc catatcacca cgtcaaaggg tgacagcagg ctcataagac
     1681 gccccagcgt cgccatagtg cgttcaccga atacgtgcgc aacaaccgtc ttccggagcc
     1741 tgtcatacgc gtaaaacagc cagcgctggc gcgatttagc cccgacgtat ccccactgtt
     1801 cgtccatttc cgcgcagacg atgacgtcac tgcccggctg tatgcgcgag gttaccgact
     1861 gcggcctgag ttttttaaat ggcggaaaat cgtgttgagg ccaacgccca taatgcgggc
     1921 ggttgcccgg catccaacgc cattcatggc catatcaatg attttctggt gcgtaccggg
     1981 ttgagaagcg gtgtaagtga actgcagttg ccatgtttta cggcagtgag agcagagata
     2041 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga
     2101 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
     2161 tcagtaagtt ggcagcatca ccaaaagaaa gcgaccaatt tttaagtatt aaaattaatt
     2221 caacagaaaa ttccacgcct ctccatgagc gataaaagtc tgtaataccg ccgatcattt
     2281 gaaatacaac aagtgcaaca agtgccatta gtacatagtg taaattaaaa aaatagtcat
     2341 ttataaaaca cgaaaaataa atacctaaaa atattatcag aatatcagaa aaacgctgaa
     2401 gcatagaaat tatggaagca tttgcattcg agcgaaagcg atgctgagat attgtcataa
     2461 ttcaatcact catttataaa caagcatatc tgtcgataag tttcattaac gtaacaaaca
     2521 agagatgaaa aactcttagt gttgccatga tatatttctt ttgataaccc ttgctggaac
     2581 gccagctata agacaatttt tctcaaggaa aacaccatta acgactgaat gactcgcaac
     2641 aacgcaacca tcagcgataa tacaaccttt attaatggca acattgttcc ctataaggca
     2701 attttcacct atggtaattt taccgtcttt aggtttctta tcattatagg aaaccaaatg
     2761 aaaatcttca tccagaaatt gaaccctcca agaaaccata ctcccttttc caatgctaat
     2821 gccagaataa gaaatgagat ctgtttcagg cccaatataa cagtcgttta aaataatctt
     2881 actagagtca gcaatgtcaa cccgaacact tttagctata aacacatttc ctgacacatg
     2941 catttcaccg cggttattta tataacatat cgaattatta tttacgtgat taacatttga
     3001 caacccgaca attaaattac tatttttgtg gaattttata ttttttatat tctttatttt
     3061 cactttcgga gaagttaaaa tgtttttttt gtagaccaat cgaaacatta gcatcctaaa
     3121 caaaacacca atgatgctac attcagaaga tcggcatcta ttttttagta tactaatgcg
     3181 ttcaataact ttattgaaaa tcatattgta gctcttatgt tcttaagttt aagaaatgat
     3241 atccattaat atagtactaa gtttgacatc aacaatttgc ccaaaacttt tacaatagaa
     3301 tttaattttc ctaatattat ccggtaattt ttcaccaaac cgtacccatg accaatcact
     3361 atagtcagag gcagaacaat tccattctcg cgtagtaata ttattatcga gagaatcata
     3421 acactcaaga gttagtttta agtcaccact aacaatcttc aacatcattc cacccgacag
     3481 tatacctgtc gatttcacca atacactttg tgataaacta gcgccgtttt ttgaaaatac
     3541 aatggtttca gaatcattgg aagaaatacg cgcctggagt gaaacaccat cattgaaatc
     3601 ccaatatctc aatccatcct taaatcttga gttcatcaac acagaagaat catttttgtt
     3661 taaggctaaa tatgacttat tatttaatga gactctaacg ttttcgagat aagacttccc
     3721 atcaccgcct actagtacta gagcattttc cgtgcctttt gatggattgt agttttcagt
     3781 tgcctggaat cgtaaattac gcacatataa accattttca ttgtcactta tcacataaaa
     3841 caatggcgta tttattattt taggtgtcgg acttataaaa ataaaactgt caataactga
     3901 agcaaagcta tttattgagt taatactaac aattgtaaaa tctgtcgttt ttcgccctgg
     3961 gttttccatg tgcgattggt ttataaagac atttgcatca ccaaacaatt taacctcatg
     4021 attatcaaat gaacattttg aaaagaacca atcaccataa tgtaattcag tataactccg
     4081 attatcagca aacatacaat tatcaaatac catgcactct ccagaattct tattgccggg
     4141 aggagcaata atatttcccc ataaaaaatg gcaatcacaa aattttatgc gccatgaatt
     4201 attatcaaaa gctagtgtag tacgccaccc ctgaatcgat actgaatcaa tcgtgaagag
     4261 cgaactattt ctttcatccc caccaatttt tattgcacaa ctatccagta aatttttaat
     4321 tccagcgcca attattgcaa tactcttgat agcgactttg acattttgaa atcgtttaga
     4381 ataatctttc tggctcgaga ataatcttat tgcaaaatct gcgttaaaat cattaaacca
     4441 ttttaatgtt gacagatccg acgaaaacca tgatgttttc gatacatcta tttcatatgt
     4501 ttttttaacc tgatagatgt gccctggaat gaatttaaga ggtaagcatt cgttgtgtgc
     4561 cgcaatatta gcccgcgata tagcatttga atcatcatta ataccatcac caatagcacc
     4621 aaaccagctg acatcaataa aatcatcaat ctttctcttc catgtacaat ttttaccacg
     4681 aatataaatt acattgtcaa ttagttcatc tgcaataaca ggtatactta caaaatatcc
     4741 cgaataaaag aaagagtcat cctgagtagc ttcttggcca ctaataaaaa cacactcacc
     4801 aataaataaa gatatgtatt gtttaatact ttcaatagta tatctcttac tctgagggga
     4861 ctcgatgaca tccttattcg agcttgcata gctggtaagg acagggaata gagttagact
     4921 tggaattaat ttttttaaaa aatatctttt tgacatgagt aatttcatta cacccccctg
     4981 aatgatttgg cagcatattt atctctttaa gagcaattga cgaaataagc ctttcatctc
     5041 agcaagtaac ttattagggc taataaaaat agttaagata taaacgcccc cacctattac
     5101 tattttcagg ataaaaaatg ttaatgagat tttatcaata taaatatcac ataccaacaa
     5161 taataaaatc ataggtagtg tatgaacgaa cggaataaaa aaactattta tatagtcaac
     5221 aatacaattc cctagcactg gcttaagtaa aaagaaataa cttaataatg cattaattgc
     5281 ctggcaaatc aaaaaaccaa tggccgcacc aaccattcca ttccagatag tagaaaaata
     5341 cagaacagga ataaatatga ttatttttac aaaattaaat ctaacactta actccatttt
     5401 agcttttgcc ataagcaatg aaccaatagg gtttgctacc atacgcattg ctccggcaat
     5461 acaaagaagt tggagcgtag gaactataaa aagccatttc tcaccaaaca ccagatatac
     5521 aaaatctttt gcaataatgc ataaaccaag caatgcaggg aaatttatat aactaatcac
     5581 gtgtaggagt ttaaagaaat taattctcag cctttcttta tcatttgtat ttttgataat
     5641 gctggaaata aaaccctggt gattataggg ctaattttag atggtggatt gactgcaacg
     5701 ttaaatgata aattataccc ccaacataaa tagcaccaag aactctagcg actatcggtg
     5761 gttattgagg agttgatata atttaatata ctatcaagcg taaggtaaat gagaataaaa
     5821 gttgtttttt aatctctcgt aatttattct tctccaagtc ggatgataat atttccgaca
     5881 acaaaactta atattacagt tctcattgaa actgtaagta aatatccaaa acagcacatg
     5941 aggcatttta gttattattc caatgagact gtcaaacaaa acccagtaag ataagaaaaa
     6001 ctttcaattt ttgacagaat atcaattcaa gttctttttg cattaacgct cggtattgct
     6061 ggccatgagg catgataata aaaacaagag cagttaattg tataagaaag cttaatctat
     6121 ccagcctcat ccatgaagaa atctcatatg acaaaatgat cgtcacaaca aataaaaata
     6181 cccccaaaat atattaaccc aattaaagct gataactcat ctaacgttat att
//
