LOCUS       NZ_CP070592             1549 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Escherichia coli strain ESC53083 plasmid p53083_1, complete
            sequence.
ACCESSION   NZ_CP070592
VERSION     NZ_CP070592.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933302
            Assembly: GCF_016939795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1549)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1549)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939795.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:10:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,794
            CDSs (with protein)               :: 4,794
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 105 of 235
            Pseudo Genes (incomplete)         :: 146 of 235
            Pseudo Genes (internal stop)      :: 46 of 235
            Pseudo Genes (multiple problems)  :: 55 of 235
            Pseudo Genes (short protein)      :: 1 of 235
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070592.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ESC53083"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p53083_1"
                     /geo_loc_name="Czech Republic: Hradec Krlov"
                     /lat_lon="50.21 N 15.82 E"
                     /collection_date="2019-09-19"
                     /collected_by="Fakultn nemocnice Hradec Krlov"
     gene            complement(join(1529..1549,1..105))
                     /locus_tag="JW305_RS01360"
                     /old_locus_tag="JW305_01360"
     CDS             complement(join(1529..1549,1..105))
                     /locus_tag="JW305_RS01360"
                     /old_locus_tag="JW305_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001431667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001431667.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGITSRMLSVIPVLFFWG"
                     /name="hypothetical protein"
     gene            608..1084
                     /locus_tag="JW305_RS01365"
                     /old_locus_tag="JW305_01365"
     CDS             608..1084
                     /locus_tag="JW305_RS01365"
                     /old_locus_tag="JW305_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          127..225
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.22e-45"
     Typing          complement(421..529)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.182"
                     /coverage="100.0"
                     /evalue="4.23e-47"
     Typing          complement(1316..1548)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.453"
                     /coverage="89.0"
                     /evalue="1.46e-85"
ORIGIN
        1 cgggatcacc gacaacatgc gagaggtgat cccgctcgcc gcaagtcgat gaccgagcgt
       61 agcgagcgaa tcgacgagga agcggaagag aaccggaagc cacattgagc acttacgcac
      121 tgatgcgggg tgtcggggtg aagccctgac caagtggtaa tcgtatcggc gtgcatgcgc
      181 ggttatacga ttacacatcc tgtcccgatt tctgaggcgt tttaacggtg aacggacggc
      241 aaaaacacga tggagctgtt gaacgtgaaa accacgaaat ctggcaaaaa tcacggctgg
      301 cgaggcttgc ataggagtaa gccagtatac actccgctat cgctacgtga ctgggtcagg
      361 gcttcacccc gacaccccca aagggcgttg cgttgcacgc aacacccttg cctagaatag
      421 atcttttacg ccgatttgta agtggttgtt tttttagtgt gttttttatt tacctgtcgc
      481 atccagggtg acacaacgtt gtcgcatcca ggatgcgaca acacgcaaca aggtgttgca
      541 cagatgtgtc atgatggctt agatttgaac caacgttagt gcatgggatt ttcagaggga
      601 aaaaatcatg tttattgatt cagaaaaacg actgaaacaa ctttcagatg aggcaaagaa
      661 aaacaccgag gatctcgaag aagcaaagaa aaattcaagg tttacacagg tatccccaaa
      721 aggttgggaa cgtgttcgag agctgctgaa ggatagccaa ggcatatcag cactgaagct
      781 gtactcattt ttagcggagc atatcgatcc tacgtgtggc gctgtcgttg cggatcagca
      841 attcctagct gaaaaacttg gagttagcag aagcacaatt attcggtggc tcaattactt
      901 agaatcaaaa aatgcattag ttagaatccc cgttgctggt aaggtttgtg cgtatgccct
      961 cgatccacat gaagtctgga agggatacaa cactacgaaa aaccatgcag cgtttgtcac
     1021 taaaacactg gtcaacaaag acggtgatat tcagcgccga atcatggcca tgttttcaaa
     1081 ttgagctagc ggcaggcgga caatcagggg ctacgtgtta acgttctgac catgattgtc
     1141 tatcctgcat tgctcttttg ccgcctcaaa atcctttgcg tgtttttgct ccccgtcctc
     1201 cagaaaaaac cgagccgcca cggttccggc agcgccttga gcgctaccgg aaaccgtctg
     1261 gggagaaccc cagaccccca tcgctgcaac accgacggtc tacgaccacc taccaaccgc
     1321 atggctgcct gttccgtgca aatgggcttt gcagggggct ggcgcggttc ctggcgacct
     1381 gaaagcccct ggttaggggt atcggcgcgc ttgacctcct gagagcctct gtaagcgttt
     1441 ttcgcgcctt ccatgccctg gcgggcatct aaaacccgtt tcgtgcgtct gagagctttt
     1501 gagcgcgttt gaggggctat ctggcgactc atccccaaaa gaacaacac
//
