LOCUS       NZ_CP070591             4087 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Escherichia coli strain ESC53083 plasmid p53083_2, complete
            sequence.
ACCESSION   NZ_CP070591
VERSION     NZ_CP070591.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933302
            Assembly: GCF_016939795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939795.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:10:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,794
            CDSs (with protein)               :: 4,794
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 105 of 235
            Pseudo Genes (incomplete)         :: 146 of 235
            Pseudo Genes (internal stop)      :: 46 of 235
            Pseudo Genes (multiple problems)  :: 55 of 235
            Pseudo Genes (short protein)      :: 1 of 235
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070591.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ESC53083"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p53083_2"
                     /geo_loc_name="Czech Republic: Hradec Krlov"
                     /lat_lon="50.21 N 15.82 E"
                     /collection_date="2019-09-19"
                     /collected_by="Fakultn nemocnice Hradec Krlov"
     gene            164..871
                     /locus_tag="JW305_RS01345"
                     /old_locus_tag="JW305_01345"
     CDS             164..871
                     /locus_tag="JW305_RS01345"
                     /old_locus_tag="JW305_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296720.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001296720.1"
                     /translation="MTSNPLIAYKSNALVEASYKLTLQEQRFLLLCISRLNSGTDVASP
                     ELQKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRA
                     QYARGEGKAQITFSDAVMPYLTQLKGQFTRVVIKNISNLSRSYSIRIYEILQQFRSTGE
                     RIIALDDFKSSLMLDGKYKDFKTLNRDLIKPCVDELNKKSDLAVTVETIKKGRTVVALH
                     FRFKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1127..3286)
                     /gene="mobQ"
                     /locus_tag="JW305_RS01350"
                     /old_locus_tag="JW305_01350"
     CDS             complement(1127..3286)
                     /gene="mobQ"
                     /locus_tag="JW305_RS01350"
                     /old_locus_tag="JW305_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_023142279.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTEYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLLATL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            3465..3719
                     /gene="mobC"
                     /locus_tag="JW305_RS01355"
                     /old_locus_tag="JW305_01355"
     CDS             3465..3719
                     /gene="mobC"
                     /locus_tag="JW305_RS01355"
                     /old_locus_tag="JW305_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     Typing          complement(2929..3287)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3288..3464
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          165..871
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1131..3373)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.599"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taaaggtgat ataatttaag gtgcttgggt gaggtaattt aaggtaaagg tgaggagatt
       61 taaggtaata ttcacaggaa aatggggggt gaaaatataa ttgctcacca atgctaaagg
      121 tgataggttt ttatttcacc tcatttttgg cctggtggtt aaaatgacat caaaccctct
      181 gatagcctat aagtccaatg cgctggttga ggccagttac aagctaactt tacaagaaca
      241 gcgtttctta ttgctctgca taagccgact taactccggt actgacgtag cttcacctga
      301 attgcaaaag acaatgacca ttacagctgc ggaatatttt gattcattcc ctgatatggg
      361 gcggaaaaat gccgaggttc agctacagga agcgatcgat cgtttgtggg acaggtcgat
      421 cattctcaag gacgacgaga agcgcgagga gttccgctgg attcagtatc gggcacagta
      481 cgcgagggga gaggggaaag cacaaatcac tttttctgac gcggtaatgc cttatctgac
      541 acagttgaag gggcaattca ccagagtcgt cattaaaaat atttctaatt tatcaagaag
      601 ttacagcata agaatatacg aaattctcca gcaattccgc agtactgggg aacgtattat
      661 cgccctcgat gattttaaat catcactgat gcttgatggt aaatataaag atttcaaaac
      721 gttaaacagg gatctaataa aaccctgtgt tgacgaactg aacaaaaaaa gcgacctggc
      781 tgtaacggtt gagacgatca aaaaggggcg cacggttgta gccctgcatt ttcgcttcaa
      841 ggaagacaaa cagatcaaga tgacgatcta gaatcgattt taaggcacct aaaacgctcc
      901 ctgctgcgtt ttttttgtgt tagtagtgcg aaggtactat tgaagtgaaa aattgcgtca
      961 aaagcgctct aatgcagcga aaaaagaacc cccatttttc gggggcggaa aaacaaaact
     1021 atgcttgcat tatgtgtgtg cttcttgcat tcatccctgt cgatgaacat cgaagaacat
     1081 cgacgaacat aacacgatgt taaacaaaac aaaatattgt tcacattcac tgtgaccaac
     1141 cgcgatcatg tctcctctcg gtggcgtgtg ttcgtgcatc ttcctcgcgc cgccattcgt
     1201 cccaggtacg attaaaaccc agcgagcgga acagatgatc cggctgtttt tctggtgtgt
     1261 tgacggcagc aaaaagcggt gcctcacggg ttttcagatg aaactccata agttccggcg
     1321 caggtgatat gtggctgtct gcatcgtgat ttacggcaac accataccct gcataccgta
     1381 accccttatc ggtcagtgca ctgatgacca gctggctgtt atcggctgag ggaacggcaa
     1441 acgcgggata cagcgtacca ttaagccgga caacaccgcc cagagagtca tccacgccca
     1501 tgaccagcaa aagctgaagc tggcgggttt cccggtcgac agaactcatt accggcatca
     1561 gcgtcgctag cagacgtccc tgcgtaaaat ccaccttcct tgctgccagt gatggcatgg
     1621 ctggctggcg gtcttctcca agacagaaag actccggcca tgtcttcggc tggcggagat
     1681 tgcgggtata ttgcggccat gtctggcaaa cctgcgtcag aagttgctca aaggtcgcag
     1741 aggaataaac acgttgcagt ggtggatgtt gaccgatttc gcttcggtca cagatataac
     1801 cactcctgtc acgcattaaa tcaacacgct caaagattct ggtgcggtat ttctccagct
     1861 cgttccccca gagcgtgtat tctccgttcc ggttgtgaag cagaaggtaa caggtcattt
     1921 ccccgttacc aggcatgggg gcctcgccat actccgtaac gactgcgctc agtacccctg
     1981 aatccataac ggcattgtca tttttcctga gctgttccgc tcgtcgctcc gcgcgactac
     2041 gttctgtatc ctcctgctcc ttccggcgca attcctcttc acgtttaagc aactggactc
     2101 tgagagctgc aaggccggat gccccccact gctgccactg tttttcaaat gccgtctgac
     2161 tcgcgggaat gtcctgtttc tccaccttcc ggtaatgctg tcgcaatttt tccatttgct
     2221 ggctcagcga ctggattttt ccctgttcgc tacggtattc cggtgccgcc cattgcatca
     2281 ggggaatacg ggaaaaaccg gacgaaaacg gcgatgattt tttcaggctg gcagcatggc
     2341 tcatcgcttc ctttcgttcg gtgttcagct cttcccagcg tctgcgcgtc gcctgcatat
     2401 ctgccatcat cgacatggca ttactggtga gtttccatgc cgcattactg tccttcggca
     2461 acgcttcgat gatgtcacgt agtgtgtacg aagttgcgtt gtcaggaagc acgatattca
     2521 gaaggtcagc cacacggcgg gcaggctctg tcagcccgct gtttctgagt tcggtacggg
     2581 aaatctgtat ttccaggtta agtctgttaa tcagacgatt ctgttcgccg cgttctgttt
     2641 cgataccgcg tgtttccatc gcacaggcag actttccgag gtgaatacca ggcgttttct
     2701 caagcccctg tcgctcgtat gaacgatggt ctatctcttc ctgataaccg gcgttcgcca
     2761 gtgccctgtt cgcatggtca gcccatgatg cacgccacgt ctcggcatgt gtccggtcgt
     2821 tccagtccct gacctttcca ccgaatccag caggtccgac agctctcgtg gtcagcatga
     2881 tgtgcgcatg gggatttgtt ttatccatgt gatggaacgc cacatcagca atcatgccct
     2941 gactgacaaa gttttctctg acaaaggcga gaacggtttc ccgtgcggca tcctgcggta
     3001 gttcgcgggg gatggcgagt tcaatttccc gcgcaagttg tgagttcttc cggcgttcgg
     3061 ccttctcaac ggcattccag agttcggcac ggttcgtaca ccaggcaggg gctgtcgcgg
     3121 gagtcagaat cacagcttca gccacaccgc ttttgcgggt gtagtcgtga gtcagtccgg
     3181 tacgctcatc aagcatcagt gaaccggaac gataggcagc agaagcaacg gcgctgtagc
     3241 cgctgtttct ggagatgatt ttcatgctga gatgataaat cgccatttct gccgtaatgc
     3301 ctttttgtgg ttttctttca gccccgcagg gcgcacacaa tcgaaacgga gtggagattg
     3361 tataagtgcg cacttcgtga ttttccgtgc attatgccag ctcagttttt aaactaccag
     3421 tgacgcgcta aaatgcgatt tttcatcgcg cacggagtta catcatgagc agaacactcg
     3481 aacagaagat tgctgatgct gaggccagat tacagcgcct gaaggcgaag agcaggagtc
     3541 tggacacagc gcagaaggtc attgtgggtg cagcattgct ggcaaaggtc agaaagccgg
     3601 aggaggtgca gttgcgtgcc tggttactcc agttcctgaa ggcagaggtg acacgacagg
     3661 ctgatgtgac gcgcatactg ccgctgatta acgagctgga agcgctgcca ggacagtgat
     3721 tgtatcgtcc gctcgccgca gtcccagcga ccgagcgtta gcgagcgagt gggcgaggaa
     3781 gcggaagtga atccgtcgcc tgattgagca catacgcacg gtttttcggg aggtgatatg
     3841 cgctgaaccc gcatgaatac tggatttcca cagggttgga gggggtgtat tcggaacttg
     3901 caattctgct tttttgcaac gcattgtttt tattggtttt ttgttttcag cactccaagt
     3961 tatccacagg ctcgtcgtta agtttttaga tcttgttttt aagatcttgt ttttaatact
     4021 tgttttagcg ccaaaattcc ttatagaaaa caaaatgtta caacgctata ggtgatatga
     4081 tttaagg
//
