LOCUS       NZ_CP070589             6895 bp    DNA     linear   CON 03-JUN-2024
DEFINITION  Escherichia coli strain ESC53083 plasmid p53083_4.
ACCESSION   NZ_CP070589
VERSION     NZ_CP070589.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933302
            Assembly: GCF_016939795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6895)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6895)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939795.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:10:12
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,142
            CDSs (total)                      :: 5,029
            Genes (coding)                    :: 4,794
            CDSs (with protein)               :: 4,794
            Genes (RNA)                       :: 113
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 235
            CDSs (without protein)            :: 235
            Pseudo Genes (ambiguous residues) :: 0 of 235
            Pseudo Genes (frameshifted)       :: 105 of 235
            Pseudo Genes (incomplete)         :: 146 of 235
            Pseudo Genes (internal stop)      :: 46 of 235
            Pseudo Genes (multiple problems)  :: 55 of 235
            Pseudo Genes (short protein)      :: 1 of 235
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070589.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="ESC53083"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p53083_4"
                     /geo_loc_name="Czech Republic: Hradec Krlov"
                     /lat_lon="50.21 N 15.82 E"
                     /collection_date="2019-09-19"
                     /collected_by="Fakultn nemocnice Hradec Krlov"
     gene            complement(<1..747)
                     /locus_tag="JW305_RS00660"
                     /old_locus_tag="JW305_00660"
                     /pseudo=""
     CDS             complement(<1..747)
                     /locus_tag="JW305_RS00660"
                     /old_locus_tag="JW305_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            <1086..>1211
                     /locus_tag="JW305_RS25485"
                     /pseudo=""
     CDS             <1086..>1211
                     /locus_tag="JW305_RS25485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            1490..1841
                     /gene="tnpB"
                     /locus_tag="JW305_RS00670"
                     /old_locus_tag="JW305_00670"
                     /pseudo=""
     CDS             1490..1841
                     /gene="tnpB"
                     /locus_tag="JW305_RS00670"
                     /old_locus_tag="JW305_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /name="tnpB"
     gene            2070..2117
                     /locus_tag="JW305_RS25490"
     CDS             2070..2117
                     /locus_tag="JW305_RS25490"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_251301874.1"
                     /translation="MRRMLFGTRSEKLRP"
                     /name="hypothetical protein"
     gene            2209..2415
                     /locus_tag="JW305_RS25495"
     CDS             2209..2415
                     /locus_tag="JW305_RS25495"
                     /inference="COORDINATES: protein motif:HMM:NF024406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="WP_251301870.1"
                     /translation="MRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQ
                     LELVSSALKVIRTERVKKSLYKM"
                     /name="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            2489..3222
                     /locus_tag="JW305_RS25500"
                     /pseudo=""
     CDS             2489..3222
                     /locus_tag="JW305_RS25500"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /name="IS66 family transposase"
     gene            3200..3352
                     /locus_tag="JW305_RS25505"
     CDS             3200..3352
                     /locus_tag="JW305_RS25505"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_251301871.1"
                     /translation="MPSVITGDDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGASVE
                     HCCTG"
                     /name="transposase"
     gene            3340..3483
                     /locus_tag="JW305_RS25510"
     CDS             3340..3483
                     /locus_tag="JW305_RS25510"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="WP_000274291.1"
                     /translation="MYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDELLPWNVVLT
                     NK"
                     /name="transposase domain-containing protein"
     gene            complement(4121..4393)
                     /locus_tag="JW305_RS25515"
     CDS             complement(4121..4393)
                     /locus_tag="JW305_RS25515"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="WP_251301872.1"
                     /translation="MSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIE
                     IFYNRQRRHSRLGNISRQPSGKNIIRWLLKKRTNGCVRYCQYTSN"
                     /name="integrase core domain-containing protein"
     gene            complement(4383..5015)
                     /locus_tag="JW305_RS00680"
                     /old_locus_tag="JW305_00680"
     CDS             complement(4383..5015)
                     /locus_tag="JW305_RS00680"
                     /old_locus_tag="JW305_00680"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_251301873.1"
                     /translation="MATTISIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKV
                     AHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPV
                     APNLLNQTFAPTAPISLGGGPDVCCHTVRDGCTSLASKDVYTCEIVGYAMGERMTKELT
                     GKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQFGPENINVA"
                     /name="IS3 family transposase"
     gene            complement(5072..6223)
                     /locus_tag="JW305_RS00685"
                     /old_locus_tag="JW305_00685"
                     /pseudo=""
     CDS             complement(5072..6223)
                     /locus_tag="JW305_RS00685"
                     /old_locus_tag="JW305_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /name="IS30 family transposase"
     gene            complement(<6288..>6542)
                     /locus_tag="JW305_RS00690"
                     /old_locus_tag="JW305_00690"
                     /pseudo=""
     CDS             complement(<6288..>6542)
                     /locus_tag="JW305_RS00690"
                     /old_locus_tag="JW305_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
ORIGIN
        1 aataactgct acgggtaagc ccagaaacat gaagtaattc tgtcagggat atgtttgcct
       61 cagggcatca gcgatctttg ttttttctct gttgttcagg tgactgatac tgatgcccgg
      121 gtcttttttt atgatttcct ccgccttttt cagaatccag ctccatctgc cggcggcgta
      181 tttcctgatt cttcgggcga cttcctcccg caacgcatcg cgttctgcct ccagggaagg
      241 ttcgttatgt ttacgcatag tctggtaagc actgttgccg ataatttatc tttctattta
      301 tacaataccg cacgctgaca ccatacgcct ggcgatctca ctggctgata cttgtcgtgt
      361 acagagtgcc atgacagcct gacgtttgac ttcggttcaa agggcgcaac gggttggcct
      421 tactgtaaat atacgtcgtc tgtcgggata aagttcatta acccagcgag cgagtacatc
      481 agtgcaggga taacccaggg ctctgctggt aaacgccagg cagcagccgt ggttaagata
      541 atgttcaacg gcaacctgtt ttttgctcat cggagtaacg gtgcttatgg acgaatggat
      601 tcttttgcgc caccacctgc ttcccatgag cgatccaacg ccgcagattt ctttcgaagg
      661 gtagcccagt tcgcgtacga cgggagccag cttcttacca tatttgaaat aaagctcaat
      721 ggcgcggatt ttttcttcag taaacatgtt tatctcctga gagtccagga aatcgtccgc
      781 atccccccaa cgagctgctg tttaaattcg ggaggataat tagggcagcc ttttcgcctg
      841 ccgggagtca catttttctg catatctgac actttggttc ccactactta tttggtggac
      901 accactttgt ctaattcgtc agattctgac cagacggttc aggctgtacg cttacagtgt
      961 tcaggagtaa gtccaaatat cgtaagcgta aactgaccgc cgtatgtagc catcagacga
     1021 gaattggtaa cttagacgcc catctgatat agacggacat ctaagtatgg aattacagga
     1081 ctggcgaaaa gaaacctcgt aaaaactatt cgaatgaatt caaacttcgt atggtggaac
     1141 tggcatcaca acctggagct tgtgttgcac agattgcacg tgaaaatggc gtcaatgata
     1201 atgttatttt tcaaatggct caggctctgg ccagaacgaa tgggcgtgtt tcgcggcgtc
     1261 ttccggtaac gacctcttct gacactggcg ttgaattatt actgtagaaa taacgccgga
     1321 tgagcagaaa gaacctgtgg cggccattgc gccgtcttta tccacttcca ctcagaccag
     1381 agtcagtgcc agttcctgca aggtggaatt ccgtcacggt aacaacgctg gaaaatccat
     1441 cgccagagct gctcacagtg ttgatccgtg aactgaccgg gaggggaaga tgagctcact
     1501 cccatcaggt acccgtatct ggctcgttgc cggcgttacc gatatgcgta aatccttcaa
     1561 cggactggga gaacaggtac aacatgtgct gaatgataat cccttctccg gtcacctgtt
     1621 tatcttccgt ggccgacggg gtgacaccgt caaaattctt tgggctgatg ctgatggtct
     1681 gtgcctgttc accaaacgcc tggaggaagg ccagtttatc tggcctgcgg tacgtgacgg
     1741 caaggtatcc attacccgct cgcaaactgg caatgctcct cgataagctg gactggcgtc
     1801 agccaaaaac atccagccgt aactcactga caatgttgta aaaaactcct gaccgcatta
     1861 taaaaacggt catgagtcag aaatacctca ttcgcatcgc agagctggaa aggttgctct
     1921 ctgagcaggc tgaagccctc cgtcagaaac cagcaactga gtctggttga agagacggaa
     1981 gccttcctgc gctctgcact gacacgtgcc gaagaaagat cgaagaagat gaacgggaaa
     2041 tagaacatct gcgggctcag atagaaaaac tgcgccggat gctgttcggt acccgttctg
     2101 aaaaactgcg tccgtgaagt tgaactggct gaggctctgc tgaaacaacg tgaacaggac
     2161 agcgatcgtt acagtgggcg ggaagacgat cctcaggttc cccgccagtt gcgacagtcg
     2221 cgccatcgtc gtccgttacc ggcacacctt ccccgtgaaa tacaccgcct ggagccagaa
     2281 gaaagctgtt gcccggagtg tggcggtgag ctggattatc tgggggaagt cagcgctgaa
     2341 cagctggaac tggtgagcag tgccctgaaa gtgatccgca cagaacgggt aaaaaaaagc
     2401 ctgtacaaaa tgtgactgta ttgttgaagc accggcgccg tcccgcccga tagagcgtgg
     2461 tatcgcgggc cccggattac ttgcccgcgt gttaacggga aaatactgcg aacatctgcc
     2521 actgtatcgt cagagtgaaa tctttgcccg ccagggtgtc gaactgagcc gggccttact
     2581 ctccaactgg gttgacgcgt gctgccagtt aatgacaccg gtgaatgatg ccctgtaccg
     2641 ttatgtaatg aacacccgca aggttcacac tgatgacaca ccggtaaagg tactggcacc
     2701 ggtgtcagaa aaggcgaaaa cagggcgtat ctggacgtat gtccgggatg atcgcaatgt
     2761 gggttcgtca tctcctccag cggtctggtt cgcgtactcg ccgaaccggc aggggaaaca
     2821 cccgagcaac acctccgccc cttccggggt atcctgcagg cggatgcgtt cacaggttac
     2881 gacaggttgt tcagtgcaga acgtgaaggt ggtgcactga cagaagttgc gtgctgggcc
     2941 catgcccggc gaaaaatcca cgatgtatac atcagcagca aaagtgcgac ggcagaagaa
     3001 gccctgaagc gaatcagtga actgtacgcc atcgaggatg aaatacgggg attaccggag
     3061 tcagagcgtc ttgccgtcag gcagcagcga agcaaagtgt tactgacgtc gctgcatgaa
     3121 tggatggtgg agaagaatgg tacgctgtcg aaaaaatcca gactgggcga agcgttcagc
     3181 tatgtactga atcagtggga tgccctctgt tattacaggt gatgacggtc tggcggaggc
     3241 ggataataat gctgcggaaa gagcgcttcg tgcagtctgt ctcggaaaga aaaactttat
     3301 gttctttggc agcgatcacg gcgcgagcgt ggagcactgt tgtacgggct gatcggcacc
     3361 tgccgtctga acggtatcga tccggaagcg tatctgcgcc atatcctgag cgtactgccg
     3421 gaatggcctt ccaaccgagt tgacgaactc ctgccatgga acgtagtact caccaataaa
     3481 taagcgtcaa tacggtgctc cgttgacgct tacaaagtat cgggaatttt ccagacaatc
     3541 ctgataatac gcttatgaac attacaacac gctcatgcgg aacctggatg ggggaaggaa
     3601 cggtgaagct gttagcgaat gactggcgtt tgtataaacg ccggtcatga tgaatgatat
     3661 tcagaatata cctcgggcta aaataccggg acagagatca ggcatgatta atctgcagca
     3721 tttttaataa cagaagtact gagcttactt tacctttgat gatggaatag ttatttccgt
     3781 cggtacttat ttttccctga gaataatgtc acggatgttc ttcctgctga tcctgcaggc
     3841 gcagtcgtaa ggtcatattt ttaatgagct gagatccggg tggcaagggg tatttgctgt
     3901 cagatactgt ggcgaactga gtctgatgtg gcgcgtatac agcccgtttt cgtacagggt
     3961 ggcctcgtta tggtgtcagt gaccagcatg tgggagacaa tgctgtattc ttttttaaag
     4021 cacccaccag aaaacggtga ttgttgtaat gataatggtc ttctcgatcg agatgtaaat
     4081 acccgtttcg ttatactgtc cgtatcggaa ccatgagaac tcagtttgag gtgtactggc
     4141 aatagcggac acaaccattt gttctttttt taagcagcca tctgatgata tttttccctg
     4201 aaggctgccg ggagatattc cccagacgag agtgacgacg ctgacgattg tagaaaatct
     4261 caatgtattc ccgtattact gagatggctt catcccggtt attaaaacga tagtggctca
     4321 ggctctcatt tttcagcgtt ccccagaagc tttccatcgg agcgttgtcg taacagttac
     4381 ctttacgcga cattgatgtt ttcaggacca aactgctcct gtatgacccg gtaatcgtat
     4441 gcgcagtact gtgaacctcg atcagagtgg tggattagcc cggcaggtgg gcgctggctc
     4501 ctgagcgcca taaacagggc tttacctgtc agctcttttg tcatgcgctc tcccatggcg
     4561 tagccgacaa tttcgcacgt ataaacatct tttgatgcca gcgaggtaca accatccctc
     4621 actgtgtggc aacatacgtc aggtccgcca cccagactga ttggtgctgt aggagcgaac
     4681 gtctggttca gcagatttgg cgcaactggc agattgtggt tcgagttcgt agtcgctctg
     4741 aacttgcgtt tctgcttaca gcgtagcctt agctccttac gaagacgtgc cagtcggtca
     4801 cgaccaacga tgatgccatt ctctgccagc tccgtctgga gccgccgggt tccatatgtt
     4861 tcgcgagtgc ggatatgtgc caccttaatc tccagtttta gccgctcatc actttgtttt
     4921 ctgtctgagg gttcatgctg tacccagttg taataaccgc tcctggatac accaaatacc
     4981 tgacacatcg cttcaatgga aattgttgtc gccattgttc gattaacgcg tatttttcag
     5041 cgactcctgt gcaaaaaacg cggttgtaga ttcaatctgt caatgcaaca cccctttcaa
     5101 ttatctcttt cggtgttttg aacttcagtg tctttctcgg tctgtgttta gctgagcagc
     5161 aaccagatct agttcatgtt gagtatattg ggcaagacat gtctttttag gaaagtactg
     5221 ccgaattagc ccatttgtgt tctcatttgt tccccgctgc caaggactct gaggatcgca
     5281 gaagtaaact ttaacgccgg tgctgacagt aaatctagat gtctggccag ttccattcct
     5341 ctgtcccatg tcagtgattt tctgagttct gacggtaaac tcaggaattt gtcggtaaga
     5401 gcctgattta ctgagacaga atctttgccc ctgagtctaa ggatgatcgt ataacgtgat
     5461 tttcggtcta caagtgtggc tatatgagag ttttttgtac ctgagactaa atcgccctcc
     5521 caatgtccca gagagcgtcc tgttatcgat atttcgggaa cgttcgtgaa ttggtgttcc
     5581 gttcactatg ttaatcgtac ctctttcgcc tttgcgggta tgacgacctg ccatggcgaa
     5641 ggctatgcga ccgtcgcaga tgctgtatat tcaggtggtg tagcgcttca cggctacgaa
     5701 agtacagcgt ttttaaattg tctcaggtga tattcgcagc gttttttgac gtggttttgt
     5761 tcgccttaac catcctgata tttgcttctg gagaccattc atctcagctt ttccagaaca
     5821 agctttcgca atggtaaatt ttgatccagt aagcacggtt ttggcctttt cgccattctg
     5881 ttggctcggt tattagcatc aacagctttg taatagcgtc tgccccgatt acgctgaact
     5941 tcacgtgaga tcgtcgaagg gactgcgatt cagcgcagta gctatcgcac gaatgctcat
     6001 tttggctgac aaaccagctc gtatctctcg cgctccagac agtgtcaggt gagctatgag
     6061 cccgcttacg ctcatggggt tttatccgcc agtatccctt aacatagtga agatcgttcc
     6121 gggttttgaa cccagatatt cgctatttca ctgaagcctg ttccgttctt ccatagttca
     6181 acaaaacaga ggctttttcc tctgctgtaa atgttcgtct cattcaaaaa acctccgcaa
     6241 ccccatgttt tcacataact gttgcgttga ccaattgaat ctacagtgct ttttttaaat
     6301 atatctcgct caaggcgagc ttcatttaac gccttacgca gttgcagaat ttcagattcc
     6361 agttcagcca cgtgcgggaa ccaggagtac cgagcctttc tggcggcggt aaccattgtc
     6421 ctaaagtgcc ttcaggaaga gattaatcgg ggaagcgccc cactgatcga aagttgattt
     6481 tcaagaaccg ttctgacagc ttcggctttg aactctttag agtaacgttg ggtttttcct
     6541 gctcattatt agctccttct gatgccattc tatttcagga aggagtgtcc gttaaactca
     6601 ggctacctca gttgcattcc agccatgatc cagaatatgt gcattttttt gcctgatagt
     6661 ttcttttttt tattcgctca tgtttaaggc ataacaggca gataaataat gtcagaaaaa
     6721 gaaagctaat aatggagagc aagagcgata tagtctgtca cagggtaata catattctct
     6781 ccggtagaag aagggccgca aacgcggccc gggctgttat cagaaagagt aacggaagtt
     6841 ggcgttgatc gcgttatcca cgtttgtatt taccaaatgc cgatttctca aactc
//
