LOCUS       NZ_CP070585             3223 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KP52813 plasmid p52813_1, complete
            sequence.
ACCESSION   NZ_CP070585
VERSION     NZ_CP070585.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933300
            Assembly: GCF_016939775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939775.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:00:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,546
            Genes (coding)                    :: 5,378
            CDSs (with protein)               :: 5,378
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 64 of 168
            Pseudo Genes (incomplete)         :: 103 of 168
            Pseudo Genes (internal stop)      :: 22 of 168
            Pseudo Genes (multiple problems)  :: 20 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070585.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP52813"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p52813_1"
                     /geo_loc_name="Czech Republic: Nymburk"
                     /lat_lon="50.18 N 15.03 E"
                     /collection_date="2019-09-03"
                     /collected_by="Nemocnice Nymburk"
     gene            complement(join(3195..3223,1..304))
                     /locus_tag="JW304_RS02310"
                     /old_locus_tag="JW304_02310"
     CDS             complement(join(3195..3223,1..304))
                     /locus_tag="JW304_RS02310"
                     /old_locus_tag="JW304_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(472..687)
                     /locus_tag="JW304_RS02315"
                     /old_locus_tag="JW304_02315"
     CDS             complement(472..687)
                     /locus_tag="JW304_RS02315"
                     /old_locus_tag="JW304_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(691..1176)
                     /locus_tag="JW304_RS02320"
                     /old_locus_tag="JW304_02320"
     CDS             complement(691..1176)
                     /locus_tag="JW304_RS02320"
                     /old_locus_tag="JW304_02320"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1211..1915)
                     /locus_tag="JW304_RS28540"
                     /old_locus_tag="JW304_02325"
                     /pseudo=""
     CDS             complement(<1211..1915)
                     /locus_tag="JW304_RS28540"
                     /old_locus_tag="JW304_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1854..2177)
                     /locus_tag="JW304_RS02330"
                     /old_locus_tag="JW304_02330"
     CDS             complement(1854..2177)
                     /locus_tag="JW304_RS02330"
                     /old_locus_tag="JW304_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(3..304)
                     /name="qacJ"
                     /gene="qacJ"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.98"
                     /coverage="94.39"
     Typing          complement(2274..2465)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          2445..2928
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(375..1864)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taataaccaa aactccaacg caaataagga acattcctgc aattgcgggc caatcaagtt
       61 tttgaccgct aaccaaccaa ccagctaggc ttattagtac aattcccgca ccagaccaaa
      121 ttgcgtaagc aataccggta ggtatatttt ccaatgtttg ggaaagcaga taaaaggcag
      181 cgcaataact tattgcagtt cctacagacg gccacaggcg tgtaaatcct tctgaatatt
      241 tcataaaggt ggtgcctatg acttcagcca caatagcccc tcccaggaaa atatatgtat
      301 tcataaaaat acctcatgtt gaaaaattta aatatcttta cataacaact aattaataga
      361 acccggctta tcatagcgaa ggaccatgat gctgccactg cgatcgttcc agctccagcg
      421 ctttctggtg ctgcaccgcc cgctcctgct gccggtgagt ttctatcgcg ttcagccttt
      481 cgacaaccgg cgcagctgct cgctcaaact ctgcacctgc ctgctcaaga ccgtgacgcg
      541 ctcgttcagc gccgcgttct cccgttgaat aagaccagac atcgtccgcc attccgcgaa
      601 ggcgttctcc cactcgtcca gccttttcga gtagtcctgc tgtagctgct ctaatgcgct
      661 cagcaactgt ttttccagct ccgtcatgtg ctatttcccc gccagtatca tccagctcga
      721 gtcctctccg aactgtcccg cttccgggtt caccctcacg cagcggcgtc tctgctcgcc
      781 gcaggtcgag agctgtacgc cgctgttcct ctctgacagc atggcctgcg tgctcttctg
      841 ctcccggatg gtcgtatagt tctcgagtat ctgctgcccc tgccaccaca gaatgcccgc
      901 gctcgacgct atcagcagcg cggagaccag aacgatggtc agccacgtct ggctgaccat
      961 gcgcagcatc cctttcgtgc gctggctcat ggctgaggac agcttccggt cgtggtcgag
     1021 gatggcggcg ctgattctct tctcgctttc gctcagttcc gctctgacag acttctcgtg
     1081 ctcgctgaat gcggctttca gcatctcgcc ggtagtctgc tgctgcgctt tcgatttctg
     1141 ctctaagtcc ttcgccagcg ttaaaagact gttcatagat ggctcccttc agtcggatgt
     1201 ttcgcccccc gtccgggtcg gcaatgctga tgctgctttt tgtggtgcgc accacctcaa
     1261 aacctgcgct ttccagcgcc tcagtgacgt cctgacgcgt tttcagctcc ccggatgagg
     1321 caagggcaag caggccgctc gtaatcgcct gggcggcttc ctgcttcgct tccggcagcg
     1381 cggaaggcga gaccagcacc cgccggttct ccggcgcgtt cgggtcatgc agccccagcc
     1441 tgccgttcac tatggtctgc caggcatcga tgcgcggacg gtcggcgcgg tcgtaatacg
     1501 gctgtagccg cttgccggtc agaagctccg tgttcgggat caggaagttc agctccagcc
     1561 gccccttgtc ccggtgctca acccacagca cgctgtactg gtctttatcg agtccgggca
     1621 tcagaacccg ctcgaaactc gccatcagct tttcgcgctg gccgggcggt aaatcctgtt
     1681 cggcaaaaga cagaacgccg gaagtgtact ttttggcgta gggcgaggcg tcgataagct
     1741 cccggacttc atccggcttc ccctgcagaa cgctggcgcc gtcgcgctgg cgatctttac
     1801 ccagcagata atcgaccggc ccgccgccgc cgccgcgccc ccgaggatga aacttaacga
     1861 tcatcatcag cacccttttc cagtacggca tgccgcaacc gctcgagtcc ggcatcgatg
     1921 gccatcagcg cggcgacaat ctgcacgcgg tcgtgtccgc ttccaccacc ggcgttaacc
     1981 tggcgggcaa tctggttaag gttgttcccc atgccggcaa gctgacgaag cagcgccggc
     2041 gagatcgacg gaagtttgcc ggcgcgtgcg ggcttctcgt ccaggcacgt ctgccgcatc
     2101 caggccgcga gttgcttacc ctcgcagcgc tccagcagac gccggtgctc gtcctcagtc
     2161 acccacatgg tgagcatttt gctgcgtttg tctgccaatc cggcctcctg ataacgccgg
     2221 gacggacggg aaatcttttc ccggtcggca cggtgttgga caagccgcag gcgcggcagt
     2281 ggttttagcg ggtttcgggg cgcagccctg aaccagtcac gtagcgctag cggagtgtat
     2341 actggctaaa catgttatga cggagtggca atttatgaaa gtgcttcatg tgacagggga
     2401 aaaatgcggc tgccgggcgt aagtgcaaaa tgcaggtggt gatgaaatga cgcctcctcg
     2461 ctcactcggt cgctacgctc ctgccgtgag actgcggcgg gcgttaccag ctcacagatt
     2521 acgggataag caaatgccgc tcgaagcagg aaaggccaag aaaaggatgc ggagttgccg
     2581 tttttccata ggctccgccc ccctgacgag tatcacgaaa atgacgctca aatcagcggt
     2641 ggcgaaaccc gatgcggctt aaagatacca ggcgtttccc cctggtggct ccctcgtgct
     2701 cccccgcatt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcggtttct
     2761 cattccacgc ctgaaactcg gttccgggta ggcagttcgc tccaggatgg actgtatgca
     2821 cgaacccccc gttcagtccg actaccacgc ccgttccggt aactatcaac ttgagtccaa
     2881 cccggaaaga cacgacaaat cgccagtggc ggtagccatt ggtaactggg ttcacggagc
     2941 agagattgta gagaatgcct acagagttct tgaagtgaaa gcctgagtgc ggctacactg
     3001 aaaggacagt ttaggtatct gcgctccgtg aaagccagtt accatcggaa caaagagttg
     3061 gtagctctga attccggcaa acgaaccacc gttggtagcg gtggtttttt tgtttacagg
     3121 gaaaaagatt acgcgcagaa agaaaggatc tcaaggaaat ccttttgctt tggccaatat
     3181 gcttcggtaa aaagttaatg cgcagaagat tttgaaaaaa tat
//
