LOCUS       NZ_CP070584             8023 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KP52813 plasmid p52813_Col4401,
            complete sequence.
ACCESSION   NZ_CP070584
VERSION     NZ_CP070584.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933300
            Assembly: GCF_016939775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939775.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:00:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,546
            Genes (coding)                    :: 5,378
            CDSs (with protein)               :: 5,378
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 64 of 168
            Pseudo Genes (incomplete)         :: 103 of 168
            Pseudo Genes (internal stop)      :: 22 of 168
            Pseudo Genes (multiple problems)  :: 20 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070584.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP52813"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p52813_Col4401"
                     /geo_loc_name="Czech Republic: Nymburk"
                     /lat_lon="50.18 N 15.03 E"
                     /collection_date="2019-09-03"
                     /collected_by="Nemocnice Nymburk"
     gene            79..2976
                     /locus_tag="JW304_RS02270"
                     /old_locus_tag="JW304_02270"
     CDS             79..2976
                     /locus_tag="JW304_RS02270"
                     /old_locus_tag="JW304_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_001553819.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            complement(3301..3510)
                     /locus_tag="JW304_RS02275"
                     /old_locus_tag="JW304_02275"
     CDS             complement(3301..3510)
                     /locus_tag="JW304_RS02275"
                     /old_locus_tag="JW304_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_040242824.1"
                     /translation="MKSIKNFVAVAALSMMSFGAFAQSVSATASTLDRAEAKIAAQAAE
                     QGASYKITSAQFNNRVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(3787..4329)
                     /locus_tag="JW304_RS02280"
                     /old_locus_tag="JW304_02280"
     CDS             complement(3787..4329)
                     /locus_tag="JW304_RS02280"
                     /old_locus_tag="JW304_02280"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_135712390.1"
                     /translation="MFSAILNTSKNSNAEKYKFLSLRIWRLVTVKTENSTTPKGFIDGV
                     LTRNAFDLRPNEKSVSFSRLETEESIPNDSLKNKKLMACKIRTANKMQNIFISEESANN
                     EDLRLDPETNELLKHISFSETENSKFSNDGITGFHWDLFFISKNGNKLRSGDSELSEKH
                     ILDAKDLLIEMSLLPNS"
                     /name="hypothetical protein"
     gene            complement(4342..5055)
                     /locus_tag="JW304_RS02285"
                     /old_locus_tag="JW304_02285"
     CDS             complement(4342..5055)
                     /locus_tag="JW304_RS02285"
                     /old_locus_tag="JW304_02285"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040242822.1"
                     /translation="MDVQPTYYTQDAEMHEIVINIRKPSTEKRGEGKSSIFYTKTSSVS
                     KTIETKSYTTHLKPFIEKKDNEEHLWLKNLEQKAKLAAELISKGESNTDIIGVITSSLN
                     QNKTLVEDLKNAIEAHKESMKNVSDKNNESEIKGPNISLLSLFNALKFYPVISTLPKEM
                     VSQYIDADAHIVGMVIGDNNNKININFMSTGELLFRHIDKTTGRVRISGSAFFGKDENI
                     KDMKKIKKLISFVKI"
                     /name="hypothetical protein"
     gene            complement(5058..5333)
                     /locus_tag="JW304_RS02290"
                     /old_locus_tag="JW304_02290"
     CDS             complement(5058..5333)
                     /locus_tag="JW304_RS02290"
                     /old_locus_tag="JW304_02290"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_040242819.1"
                     /translation="MSKGGALAKRAKTQNDSHGGRDLIIRKMEPSFPFAIRSIQADGII
                     ILDFIGDEISPNKREAFASVLLTKTIAQDLKSKIDGYLAHQENNEE"
                     /name="hypothetical protein"
     gene            6219..6410
                     /locus_tag="JW304_RS02295"
                     /old_locus_tag="JW304_02295"
     CDS             6219..6410
                     /locus_tag="JW304_RS02295"
                     /old_locus_tag="JW304_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040242829.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDDN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6410..6811
                     /locus_tag="JW304_RS02300"
                     /old_locus_tag="JW304_02300"
     CDS             6410..6811
                     /locus_tag="JW304_RS02300"
                     /old_locus_tag="JW304_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040242827.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGARNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7402..8007)
                     /locus_tag="JW304_RS02305"
                     /old_locus_tag="JW304_02305"
     CDS             complement(7402..8007)
                     /locus_tag="JW304_RS02305"
                     /old_locus_tag="JW304_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509965.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGCAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     Typing          complement(5692..6098)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.311"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttttgtatcg gaacgccctg atttatatgg gctggctgtt gtccaaaaca gactatactt
       61 caaaagggac gaatttgtat gtcacgacgc catattttca ccgaacggca gcgagcagcg
      121 ctgttcgatc tgcccacgga cgaactgtcg ctactgaagt tctacacgct gggcgatgat
      181 gacctggaaa acattaggca gcgccgcaga ccggaaaaca ggattggctt tgccctgcaa
      241 ctttgtgcct tacgatatcc gggccgtgca ctggctcctg gtgagatgat cccgcgtgaa
      301 gtcctttcct tcgtcggtgc tcagcttgga gttccggctg atgcgcttct cacttatgcc
      361 acacggcgcc aaacccgtca gcagcacatg gacacgctgc gcgaaattta cggctacaag
      421 accttcacgg gccgtggtgc ccgtgatctg cgggagtgga ctttcggcca ggccgaagat
      481 gccagatcaa acgaggatct tgctcatcgt tttattgtgc ggtgtcggga aacttccacc
      541 attctgcccg cagtatcgac aatcgagcgc ttgtgcgcgg atgctctggt cgccgctgag
      601 cggcggattg aaacgcggat tgcggaaaat ttaacagcgg atgttcgcga tcacctggac
      661 aaacttctga gtgaaatgct cgccggcaat atcagtcgtt tcatctggct tcgcaacttc
      721 gaggttggta acaactcggc tgctgctaac cgtttgctcg acaggctcga atttctgcgt
      781 accctgaata tcaatcatag tgctttggcc agcatacctg cccatcgcat tgcccggctg
      841 cgtcggcagg gtgaacgcta cttcaccgac ggtttgcgtg acatcacttc ggaccgccgc
      901 tgggcgatcc ttgccgtctg tgttgtggag tgggaagcgg cgattgctga tgccatagtc
      961 gaaacccatg acaggatcgt aggaaaaacc tggcgggaag cgaagcgcca gcatgacgaa
     1021 acaatttccg gctctaaagc cacactcacg gatacgatcc gtaccttcac cgcgctggga
     1081 gcttcgttgc ttgaggcccg cagtgacgga accccgctgg agatggctgt cgccagttcg
     1141 gttgcatggg accggctcgc tcaactggta gcgacaggga ctcaactcag caacacgcta
     1201 gccgatgagc ctcttgcata tgtcgggcag ggataccatc gctttcgtcg ttatgcgccc
     1261 cgcatgttgc gctgtctgaa gctcgaagcc gcgccggtcg ccggaccatt ggtagcagca
     1321 gctttgtcga tcggagagat gaaaggtgtt gcatcgccag aaaggcgttt cctgcggccc
     1381 agctccaaat ggaaccgtca tttacgagct caggaaaaag gagatacccg tctttgggaa
     1441 gtggcggtac tctttcacct ccgggatgct tttcgttccg gagatgtctg gctcgctcat
     1501 tcgcgccgct atggtgacct caagcaggta ctggtgccga tgatcgcggc gcaggaaaat
     1561 gcaaaactgg ccgtgccttc caacccacag gattggctgg cagacagaaa ggcgcgactc
     1621 acgatcgctc ttaagcggct ggcccgggct gcccgtaacg gcactattcc gcacggtagc
     1681 atagaagatg gaacgttgcg gatcgacagg ttgacagcag acgtgccgga tggtgccgag
     1741 gcactcatac tggatctgta tcgccgaatg ccgtccgttc ggattaccga catgctgctt
     1801 gaagttgatg cagcccttgg tttcacagat gcgtttaccc atctgagaac cggggctcca
     1861 tgtcgcgacc ggatcggtct gctcaacgtc ctgctcgctg aagggctcaa tctgggcctg
     1921 cgtaagatgg cggaagctac aaacacgcat gattactggc agctctcacg ccttgcccgc
     1981 tggcatgttg aaagcgaagc catgaaccag gcattggcaa ttgtggtggc cgcgcagggt
     2041 aaactgccga tgtcacgcgt ctgggggatg ggcacgtcag catcgagcga tggtcagttt
     2101 ttcccgacag cgcggcatgg cgaagccatg aacatggtca atgccaaata tggttctgtt
     2161 cccggcctca aagcgtatac tcacgtaagc gaccagttcg cgccattcgc ttgtcagtcg
     2221 atcccggcga ccgtgagcga ggcaccgtat attctcgatg gactactgat gaacgaggtc
     2281 ggtcgccatg ttcgcgaaca gtatgccgat acagcaggat tcaccgacca tttgttcgga
     2341 gccagtagcc tgctcggcta caatctcgtt ctgcgaatca gggatctgcc atcgaagcgg
     2401 ttgtacgtat ttaatcccga tacgaccccc agggagttac gcaagttggt aggtggaaaa
     2461 gcccgggagg atcttatcgt tgcgaactgg cctgatattt tccgttgtgc cgcgacgatg
     2521 accgctggca aaatcaggcc cagccaactc ctgcgcaagc tcgcttctta cccacgacaa
     2581 aacaaccttg cagttgcgct tcgtgaagtt ggtcgtattg aacggaccct tttcattatt
     2641 gagtggatcc tggatacgga catgcagcgg cgtgctcaga tcggtcttaa caagggagag
     2701 gcccaccatg cgctcaaaaa tgcgctccgt atcgggaggc agggggaaat tcgcgatcgc
     2761 acgacagagg ggcagcacta ccgaatcgct gggctcaatt tattgactgc ggtgatcatt
     2821 tactggaata ccgtccatct tggtcatgcc gtcacggagc ggcggaacga agggttggat
     2881 gttccccctg aatttcttcc ccacatatcc ccattgggct gggcgcacat tctactgact
     2941 ggcgaatatc tttggcccaa ggaaccgaaa gcttagggtg tcatttcgcc ctcagccgga
     3001 accgacccct accctggctg aatggcgtga attttcgcgc tatgcacttg cgtgcatact
     3061 catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc
     3121 ctgttttacc ggtttcccgt cagaaacgcc ctgaggccgt tttagcggtg cgtacaatca
     3181 tgcctttatg ttaaatgaca gatgcaaaaa agcccccgaa ggggcctgga ggtttttctg
     3241 ctaagggacg aaacgggccg cctcccggcg acctgttcgg cagctccctc tgcgaccatc
     3301 ttatttggtc agttcagccg tcatgtggac acggttattg aactgagcgc tggtgatttt
     3361 gtatgacgca ccttgttcag cggcctgtgc ggcaattttt gcttcagcac gatccagggt
     3421 ggaggcggta gcgctgacgc tctgagcaaa ggcaccgaaa gacatcatgg atagagcggc
     3481 aactgcaacg aaatttttga tggatttcat ggtcgtattc attaagttat tttgttctgt
     3541 atttggcgtt gttgccatga tgtgataaat gataggacga ccatgatctg attaaaatcg
     3601 aaaaaagttg cggatgttat tcaaaaaaac agaataaaaa ttaagcgcgt caaaacaagt
     3661 gtgagcctct ggtttcagcg aacagaatga agagcatcga gagttcgtac aattagagga
     3721 ggatgttaaa ttaccttctt gtatttattc ctaatgttgg ctattcctgc gactaagtgg
     3781 aggttattac gaatttggta gtaatgacat ttcaatcaac aaatcctttg catctaatat
     3841 gtgtttttca gacaactcgc tatccccaga gcgcaactta ttgccatttt tagatataaa
     3901 aaacaagtcc caatgaaaac cagttatacc atcattcgaa aacttactat tttctgtttc
     3961 tgaaaaagag atgtgtttaa gcagttcgtt tgtttctgga tctaatctca aatcttcatt
     4021 gtttgcagat tcttcactta tgaagatatt ttgcatttta tttgctgttc taatcttgca
     4081 tgccatgagt tttttgtttt ttaacgagtc atttggtatg ctctcttcag tctcaaggcg
     4141 tgaaaaagat actgattttt catttgggcg gaggtcaaat gcatttctgg ttaacacgcc
     4201 atcgataaat ccttttggag tggtgctatt ttctgttttt acggtaacta aacgccatat
     4261 tctgagagaa aggaatttgt atttctctgc attgctgttt ttgctagtat tcaaaatggc
     4321 agaaaacatt agttctatct cttatatttt tacgaaggaa atgagttttt ttattttttt
     4381 catgtctttt atgttttcat cttttccaaa aaaagcactc ccgcttatgc gtactctacc
     4441 tgttgttttg tcaatgtgcc taaataaaag ctctcctgta gacataaagt ttatatttat
     4501 cttgttatta ttgtctccaa taaccatccc aacaatgtgc gcatctgcat caatgtattg
     4561 agataccatt tcttttggca atgttgatat tacaggatag aatttgagtg cgttgaatag
     4621 agatagtaaa cttatattag ggccttttat ttcactttca ttatttttgt cactaacatt
     4681 tttcatcgac tctttatgag cttcaatggc gttctttaag tcttcaacga gtgttttatt
     4741 ttgatttaaa cttgaagtaa ttactcctat gatatctgtg ttgctttctc ctttggatat
     4801 taattctgcg gctaattttg ctttttgttc gagattttta agccataagt gttcttcatt
     4861 atcttttttt tcaatgaatg gtttaagatg agtagtgtag gatttagttt caatggtctt
     4921 agatacagat gatgtctttg tataaaaaat agaactttta ccttcacctc ttttttcagt
     4981 gctaggcttt ctgatattga tcacgatttc gtgcatctct gcatcttgag tgtaatatgt
     5041 tggttgcacg tccatattta ctcctcgtta ttttcttgat gagccaaata cccatctatt
     5101 ttagacttta agtcttgagc aatggttttt gttaataaaa cggacgcaaa ggcctctctt
     5161 ttatttgggg atatttcatc accaataaag tctaatatga tgataccatc agcttggatg
     5221 cttcgaatag caaatggaaa agatggctcc atttttctta ttattaaatc tcgcccgcca
     5281 tggctatcgt tttgtgtttt tgcacgttta gccagtgccc cacctttgct cattttttgt
     5341 cctttttttg agttgtcatc atactgataa acatatctag tgatgttata ccttatctat
     5401 cataacttac atgtagattt atagtacctc agcttgaaag gtgctccttt ctggtatgaa
     5461 aggttaatgt tatatgtgta aagtaaagga tcttcttgag atccttccct tctcatcgta
     5521 atcttttatg cagaaaacga aaaaaccacc cggcaaggtg gtttttcgaa ggttcagtaa
     5581 ccggtaagtt tctgaaccaa ggtaactggc ttggaggagc acagtcacca aaacttgttc
     5641 tttcagttta gccttaactg gcgcataact tcaagactaa ctcctctaaa tcagttacca
     5701 gtggctgctg ccagtggcgc ttttgcatgt cttaccgggt tggactcaag acgatagtta
     5761 ccggataagg cgcagcagtc ggactgaacg gggggttcgt gcatacagtc cagcttggag
     5821 cgaactgcct tcccggaact gagtgtcagg cgtggaatga gataaacgcg gccataacag
     5881 cggaatgaca ccggtaaacc gaatggcagg aacaggagag cgcacgaggg agccatcagg
     5941 gggaaacgcc tgatatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca
     6001 gctttgtgat gcttgtcagg ggggcggagc ctatggaaaa acggccgctt agcggccttt
     6061 ccttttcccc gtcacgcgac cgctcggttt tctcgagctt ttatacgcat gctcctgccg
     6121 ctcgccgcag ccgaacggca ggagcatagc gactgagtga gcgaggaagc ggaaaagaga
     6181 acatccctac actgagcact gacgctctga ggcctcttat gacgaaacaa cagaaaacag
     6241 cgctgaacat ggctaaattc atccagaatc agtctctgct cctgctggag aagcttaacg
     6301 agcttgatct tgatgtcgag gccgaccttt gtgagaaact ccacgatgat gcggagcatc
     6361 ttttccgtac actgtcctcg agactggatg cattacagga tgataactga tggccagagt
     6421 gtctattagt gaggctgccc gactgacggg taagagtaga acaacactgc acaggttaat
     6481 taaagcaggt gacctgtcca cctgtagcgg tgcacgtaat gcaaaaatgg tagatacttc
     6541 agagctttta cgtgtctttg gtccctttga gcaaccgaag ggtgaacacc attctggaca
     6601 ggtctctgaa cagcgtgata cagcctctgc tgaccagagt gaacaggtga ttcgtcatct
     6661 gaaacaggag attgaacacc tgaacacctt acttttgtcc aaggattcgc acattgacag
     6721 cctgaaacag gccatgctcc ttcttgaaaa tcaccaggca aaattaacgg aatccccggc
     6781 gccatggtgg aaattctgga aaaaatcctg accccgtcat gcttcagttt tcctctccgg
     6841 ccatcccggg gagttcctga accttcccaa aaatcgtcag atctctgaac gctcctcacg
     6901 actaaaaata cccacacact acgcaaaatt aaaattttgc agatttgtat ttaatatcag
     6961 tagcttactt tcctgaaatt tcggttttta aattttattt acatagatcc gactctatga
     7021 cgagtcgtca tagagtcccc ggcgagtcgt catagagtcg gtaacgactc agggctataa
     7081 aaacaatgag ttaataacga cccgactcta gacccgactc cccgtttggt tgttttttag
     7141 actcggcgac tctaggtacg actctagtga agagtcgccg gtgaatcgtg tggtgagtcg
     7201 tccggcgact cgccataacg tgattgccgg ccgtcgacga acacgattcc tgaacgtgct
     7261 gaaaaccggt tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc
     7321 cgttcaccgt tttcggatgg gaagaacgcc ggaaacagga cttaccctgg ggtcggttcc
     7381 ggctgagggc gaaatgacac cctaagcgtt agctctgtgt cgttgcacga tgtcagcgac
     7441 ggtattcttg ctgataccga gctcgcgtgc gatccagcga tagctgcgtc cctcggccct
     7501 catcgcaacc accttaggca aaagtcggtc tgattttggt cgcactccgg cctgacgacc
     7561 aagcctctta ccacgtgcct tcgcaacagc aaggcctgac ttgacccgct cgctgatgag
     7621 atcccgctca aactccgcaa tgccggaaag aaacgtcgcc agcattcgtc catacggcga
     7681 cgaaagatcg aacgccattc cattcatggc tatcacggaa accttccagt tctccagttc
     7741 acgtagcgta ttgagcagat cgagcgtcga gcgcccccac cgggaaagct cagtgaccag
     7801 gattgcatca atttgtctgg actgggcaag cgccaggact ttctttcgct cggcccggtc
     7861 gagtttagtt cctgaacctg tttccttaaa tattcccacc acgtcgtagc cggcacagcc
     7921 ggcgaaggct cgcagatcaa attcctggcg ttcacaagac tgatccgctg ttgaaacccg
     7981 gcagtaaatg gcggcacgat gtcccaattg aaccctcctg gat
//
