LOCUS       NZ_CP070583             9248 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KP52813 plasmid p52813_ColRNAI,
            complete sequence.
ACCESSION   NZ_CP070583
VERSION     NZ_CP070583.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933300
            Assembly: GCF_016939775.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9248)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9248)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939775.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 03:00:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,667
            CDSs (total)                      :: 5,546
            Genes (coding)                    :: 5,378
            CDSs (with protein)               :: 5,378
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 64 of 168
            Pseudo Genes (incomplete)         :: 103 of 168
            Pseudo Genes (internal stop)      :: 22 of 168
            Pseudo Genes (multiple problems)  :: 20 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070583.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9248
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP52813"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p52813_ColRNAI"
                     /geo_loc_name="Czech Republic: Nymburk"
                     /lat_lon="50.18 N 15.03 E"
                     /collection_date="2019-09-03"
                     /collected_by="Nemocnice Nymburk"
     gene            join(7451..9248,1..122)
                     /locus_tag="JW304_RS02215"
                     /old_locus_tag="JW304_02215"
     CDS             join(7451..9248,1..122)
                     /locus_tag="JW304_RS02215"
                     /old_locus_tag="JW304_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_205461631.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGRREIPLQRFPVLAKTDPLAEFRPPADGGGPKADDDF
                     AGIY"
                     /name="TraM recognition domain-containing protein"
     gene            122..853
                     /locus_tag="JW304_RS02220"
                     /old_locus_tag="JW304_02220"
     CDS             122..853
                     /locus_tag="JW304_RS02220"
                     /old_locus_tag="JW304_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            860..1390
                     /locus_tag="JW304_RS02225"
                     /old_locus_tag="JW304_02225"
     CDS             860..1390
                     /locus_tag="JW304_RS02225"
                     /old_locus_tag="JW304_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1418..1597)
                     /locus_tag="JW304_RS02230"
                     /old_locus_tag="JW304_02230"
     CDS             complement(1418..1597)
                     /locus_tag="JW304_RS02230"
                     /old_locus_tag="JW304_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1666..2043
                     /locus_tag="JW304_RS02235"
                     /old_locus_tag="JW304_02235"
     CDS             1666..2043
                     /locus_tag="JW304_RS02235"
                     /old_locus_tag="JW304_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(2105..2404)
                     /locus_tag="JW304_RS02240"
                     /old_locus_tag="JW304_02240"
     CDS             complement(2105..2404)
                     /locus_tag="JW304_RS02240"
                     /old_locus_tag="JW304_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2394..2657)
                     /locus_tag="JW304_RS02245"
                     /old_locus_tag="JW304_02245"
     CDS             complement(2394..2657)
                     /locus_tag="JW304_RS02245"
                     /old_locus_tag="JW304_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(2963..3382)
                     /locus_tag="JW304_RS02250"
                     /old_locus_tag="JW304_02250"
     CDS             complement(2963..3382)
                     /locus_tag="JW304_RS02250"
                     /old_locus_tag="JW304_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            3784..5469
                     /locus_tag="JW304_RS02255"
                     /old_locus_tag="JW304_02255"
     CDS             3784..5469
                     /locus_tag="JW304_RS02255"
                     /old_locus_tag="JW304_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5479..5736
                     /locus_tag="JW304_RS02260"
                     /old_locus_tag="JW304_02260"
     CDS             5479..5736
                     /locus_tag="JW304_RS02260"
                     /old_locus_tag="JW304_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            5821..5970
                     /locus_tag="JW304_RS02265"
                     /old_locus_tag="JW304_02265"
     CDS             5821..5970
                     /locus_tag="JW304_RS02265"
                     /old_locus_tag="JW304_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1..9248
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(6074..6652)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          123..850
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 gccgggaaat cccgttgcag cgtttcccgg tgctggcgaa aaccgatccc ctggcggagt
       61 tccggccccc ggccgacggc ggcgggccga aagcggatga tgattttgcg gggatttact
      121 gatggcgctg gagctgtaca acgtcagcca cgcgaaacga caggcgcgga acgcggagaa
      181 aacccggctg accctgcgct ggttgcggga agagctgtgt tccacggcgg agctggttgc
      241 gcggcggctg gggattgcgg cggtgcagcc ggtctaccgc ttcctcgaca gcctggtggc
      301 gaaagggctg ctggtccgga ccaaatatcc ggtggacggc cgtcaggtca gcgtgtgggg
      361 gctgacgccg catggcgtgg cgttctcgtt cgacgaggac gaaccgctgg cggatgttat
      421 tccgtttcag ccgtcaaggg tgtcggcagc acatctgccg caccggctgg ccgtgcaatc
      481 cctgcgcctt gatatggaag ccagaggggc caccggctgg cgcattctgc accgtctgag
      541 tcttaaaggc atgaaagtgc cggacgccct ggcggagctg gacggtaaaa cggtggcgtt
      601 tgaggtggag cgcaccgtca agagccgtcg ccggtatcag gaagtggtgt ccgggtatct
      661 gttcaaccgc aaagctaacg gcatcgatga aatctggtac atctgcccgg accgggcgac
      721 gcaggtcagg gtgcagcggg caatcctgtc ggtggatgaa atcgtgaacc cacagaccgg
      781 cgaggcccgc aaaacggcag agctggatcg tgaacggttg tttgcgtgct ttaaatttat
      841 gacaacggag tgagggatta tggcgctgta catggtattt attgaagacg cggaggaccg
      901 gaacggcgag cgctgcatca tgccgaccag cgagcatctg gaagatatgg tggtgctcgc
      961 ctggcagacc ggggcgtatg ttttctgttt tgtcgaccgg gcccagcatc tggcgatgat
     1021 gaaatccatc gggatgtatg acgagcggct caacagcgac gaaaaagcca cgtacttttt
     1081 cactggtaaa aatccgcaat gggaacggaa caaacccaca ggaatgaagc tgtgtgatta
     1141 cctctatcgc aaggtggagc ggttcgccat cgagaacggt gtgcaggtgg ttgatgccgg
     1201 gaagtgctca ccggagcaga ttaacgcgat gattgacgtg gtgagtgagc ggcacgggga
     1261 aagcgcggtg aacgagaagc tgaaagcgga gctgatgcgg gaatataaca gccgctattt
     1321 taacgtgccg gtacctgaac tgccggagga caaacctgcc cgccggtggt ggccgttcgg
     1381 caaaaaataa atgccagcac cggccatgcc ggtgctgtta ttcctccccg agcttatccc
     1441 gcatcacctg ccagagtgtt tccgcatcct cgtgtagccg ctcgcacatg tcggccgggg
     1501 tatccatatc cagctcgttc agtttatcga gcaatagcag cgactgctcc ttaataaatt
     1561 tagccatatt gagggctgtt ttttcctgtt ttgtcatgat tctttccttc cgggcatggt
     1621 gtgcgcctgt caggtatact gtctcaaaaa tagcggggtg agacaatggc gtgggtgacg
     1681 gtgagacagg cgacggaact gacgggtaaa gcccgaagtt ccctgtatcg ggatatggcg
     1741 aaaggccgcg tatcataccg tacggaagct gatggcggcc gggtggtgga taccagcgag
     1801 cttatccggg tgtacggtga gttaagacag gatgagactc acgagcggga cgagttgaga
     1861 ctgtcgggtg agactgaaaa aatggcctcc gacgcgttga tcgcggagat aaaagcactc
     1921 agggaggagg tggccgggct acggcaggaa atgcagtcca tgagactgct ggaacacaaa
     1981 ccggagacag cagagacccc ggccggaaca ccgcgccggt ggtggccgtg gggaaaacgc
     2041 tgacgcgctg tgcttgtcca accggcgacg gccacgcaat gaattgcgtc cccgtcaccg
     2101 gttattagag aacaatatgg ggattctggc gctggccgac cacggccaga acccggactt
     2161 ttctggtctg gtcatcaaca tcatagagga catgctgtcc catcagactg atccggcgaa
     2221 cgtcctcgcc atagcgtgag tccacttcat agagtcgcgg gaagtcggcc agtttgtccg
     2281 ccgcagcgag aacccggtcg gtaacgttct gcgcatccca cagggatgaa ttttccgcga
     2341 tatagtcaaa cagcgtcgtt acatcctcga tggctccctg cttccattcc acgtcatacg
     2401 gcatcgcgca gttcctgata acggccatgc aggcgctggc ggagttgttc cgtggtcata
     2461 cccggacgcg tgtcagcacg ggcggcggtc actttggcgc gaacctgatc ttcatactct
     2521 gcctgggcca gttcttcgtt agccagtcgc tgcaacagcg cagctttgtc cgggccacct
     2581 tccggcagta tgcggggcag gctttcagcc agttcccgaa gaaagttggc gttttcgtag
     2641 tgtgtagtgt tgctcattgc ggagcctccc gggttaatgc ggcaatatga aggaagttta
     2701 gcagggggcg gtctgaaaga atatggtcgt gtcagtcagg ctgatgaagc agaaagtcgg
     2761 taaaaaaggg cttacggcat ccattttacg tcaaaacata tgccatgcag ttgcgtgcat
     2821 aggcatgcat taggataaaa tttatcgggc gcgtttccgg cagttttccg ggggggttgt
     2881 tgcctgtttt atcccgtagc cgccggaaac gccctgagcc cgtctgagcg gtgcgcgtaa
     2941 tgacgcgtta tggtaaatcc acttactgtc tgccttcata gccatcgaga taaacagctg
     3001 aaatggtgtc agccagtagc cttgattttt tcggtgtgag gtctttgctg cggtaatcgg
     3061 tacccgcaaa ttgtgttgtg ccttggagtg agtttaaaaa ttcctgactg tgaaactgct
     3121 gaatgcgttg ctgcacatca ctccgtgatt ttccggtcgc tttatccttt gcgcccatat
     3181 cccagaactg ccggaaaaaa ggcaccatca ttcgtgttgt gtcagctttc tgggtggaaa
     3241 aattgggatc aaaaccgtca tctgaagcat atacgtaatg ccgggtatgc tgttccacgc
     3301 tgccctttgg agcacaggca gtgagagttg ctgtcgctgt tatcatggcg ataagacaca
     3361 gtttgctgcc ggaacgtctc atgagcgtct ccttaccttt aattcttttt tctcattgag
     3421 ttatatatcc gcaggctatc ttatcatcag tttctgaccg tgttcagtga ccgaacaacc
     3481 cgaaccagac ccccgtttct tttcctgtcc cggagggggc tttctggtat ctcatataat
     3541 gatttcagta aaacatactg aggccggaga acggcgccgt ttgtgctggt ttttgttgca
     3601 tctggagggg attgcgcatg gtgtgagtgg ctgttttttt gatcattttc acaaaaacgc
     3661 aaatttacag acttgacatg aaaaacaagg cagcgtaatt tttatactgt atataaaacc
     3721 agtgtttata tgtacagtat tttattttag tttattgttt taaaagtcaa agaggaaaag
     3781 tatatgagtg gcggagacgg acgaggtccg ggtaattcag gtctgggaca caatggtggt
     3841 caggccagtg ggaatgtgaa cggtacatca ggtaaaggtg gcccttcatc aggtggtggt
     3901 acggatccaa acagcgggcc gggctggggt acgacgcata cacctaacgg ggatattcat
     3961 aactataacc cgggggagtt tggtaacggc gggagtaaac ccggtggtaa tggcggtaac
     4021 agcggcaatc atagcgggag ctctggtggc ggacagtctt cggccaccgc gatggccttc
     4081 ggtctgcctg ccctggccac tccgggggct gaaggactgg ctttatccgt ttccggcgat
     4141 gcgttgtcgg ccgctgttgc tgatgtgctg gctgccctga aaggtccgtt taagtttggt
     4201 ctgtggggga ttgcgattta cggagtgctg ccttctgaga tagcaaaaga tgatccgaaa
     4261 atgatgtcaa aaatcgtgac gtcattgccg gcagacacgg taacagagac gccggtcagc
     4321 tccctgccgc tggaccaggc gacggtcagc gtcactaaac gtgtggcgga tattgtgaag
     4381 gacgagcggc agcatattgc ggttgtcacc ggccggccaa tgagtgttcc tgtggtggat
     4441 gcgaaaccga caaaacgtcc gggggtattc agtgtgtcga ttccgggtct cccgtctctg
     4501 caggtgagcg tacctaaagg tgttccggcc gcgaaagccc cgccaaaagg cattattgct
     4561 gaaaaaggtg attcacgtcc ggctggtttt acggccggtg gtaactcccg tgaggccgtt
     4621 attcgtttcc cgaaagagag cggacagaaa ccggtctatg tgtcggtgac ggatgttctt
     4681 actccggcac aggtaaaaca gcgtctggag gaagaaaagc gtcgccagca ggcatgggac
     4741 gctgctcacc cggaagaggg gctgaaaaga gagtatgata aagcgaaagc tgagctggat
     4801 gctgaagata aaaatattgc gaccttaaac agtcgcattg catcgacaga gaaggcgatc
     4861 cccggtgcaa gggctgctgt tcaggaagcc gataaaaagg taaaagaggc agaggcaaat
     4921 aaagatgatt ttgtgactta taacccgcct catgaatatg gctccgggtg gcaggatcag
     4981 gttcgctatc ttgataagga tattcagaat cagaatgaga aattaaaagc tgcccagaca
     5041 tctttaaacg aaatgaatga atccttatcc agggataagg ctgcgctttc cggggcgatg
     5101 gagagccgga aacaaaagga gaaaaaagcg aaggatgcag aaaataaatt aaatgaggaa
     5161 aagaaaaaac ctcgtaaggg aactaaagat tacggccatg attattttcc tgatcccaag
     5221 actgaagata ttaaggggtt gggagagttg aaagagggta aacctaaaac ccctaaacaa
     5281 ggtggtggtg gtaagcgggc ccgatggtat ggtgataaaa agcgtaaaat ttatgaatgg
     5341 gattcccagc acggtgagct tgaagggtac cgcgccagtg atggcgaaca cctcggggca
     5401 ttcgatccaa aaacgggtaa gcaggttaaa gggccggatc caaaacgaaa cattaaaaaa
     5461 tatctttaag aggtaaatat ggggcttaaa ttacatattc attggtttga taagaaaacc
     5521 gaagagttta aaggtggtga atactcaaaa gacttcggtg atgatggttc tgtcattgaa
     5581 agtctgggga tgcctttaaa ggataatatt aataatggtt ggtttgatgt tgaaaaatca
     5641 tgggtttcga tattacagcc acactttaaa aatgtaatcg atattagtaa atttgattac
     5701 tttgtatcct ttgtttaccg ggatggtaac tggtaagcaa aagttatcaa ggatgatttg
     5761 aaatacagac ttccggtgtt cagggatgaa tgccggggtt tttaaataca gggaaggtat
     5821 atgaaaaagg taaaggttgt ttttcttttt atattgattt tttcaggctt tcttctggtc
     5881 gcatgtcagg caaactatat ccgggatgtt cagggtggaa cggtggcacc atcgtcctcc
     5941 tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt
     6001 tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga
     6061 tcgaaggtta agtcagttgg ggaactgctt aaccgtggta actggctttc gcagagcaca
     6121 gcaaccaaat ctgtccttcc agtgtagccg gactttggcg cacacttcaa gagcaaccgc
     6181 gtgtttagct aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg
     6241 ttttgcgtgc ttttccgggt tggactcaag tgaacagtta ccggataagg cgcagcagtc
     6301 gggctgaacg gggggttctt gcttacagcc cagcttggag cgaacgacct acaccgagcc
     6361 gagataccag agtgtgagct atgagaaagc gccacacttc ccgcaaggga gaaaggcgga
     6421 acaggtatcc gggaaacggc agggttggaa caggagagcg caagagggag cgattcatcg
     6481 gaaacggtga tgatctttaa gtcctgtcgg gtttcgccac tcctgactga ttcatggttg
     6541 agccaccggc tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact cagtcaggag
     6601 ggcggagcct atggaaaaac gccacctgcg tggccttgcc gttttgcctc acatgttagt
     6661 cccctgctta ttcacggaat ctgtgggtaa ctttgcatgt gtccgcagcg cccgccgcag
     6721 tctcacggcc ggagcgtagc gaccgagtga gcgaggaggc gcaatttccc cggcacctac
     6781 aagacggaca ccggacgcgc tgatgtggca tattgtcccc tgattaagtc tttatattcc
     6841 ctgataataa attaccagga taggtgaatc atatcggttt ttcgctgaac attttacgtt
     6901 aacctatctc tgttgctttt ttctccttac ggagaaatac aggtcatccc cgcgagggcg
     6961 tggctgacct cagggggata tcccctgctt acgctggctg gacgccaccg acgcacggtc
     7021 tctccccctc tttctggctc cgtccggctg gacgccggcc acgccagaaa ttcacccggc
     7081 ccccgtcagg ctcacgcctg accacccaca aaaatccacg gtgctggacg cacctggcga
     7141 agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc tcgctatggc gaactctgac
     7201 gggggccgga acaccggaaa agaccgcact ggggtgccgc cttttcctct cacacgctgg
     7261 ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca tggggcggaa agaggctggc
     7321 ctccgtggtg aaagctgaac gcaatcacca cggacgtgac cgccgccgcc atgccttggg
     7381 gcatgtccgc ggcttgtgca gcgggggctg cacatcacgc cggcaaacca tcgtctgaag
     7441 gagagagaac atgtttaata cggattcgct tgcctggaag tggcacattc cggagctggc
     7501 ccggcagatt tatccgtggg tcagctggct gtaccaggag tattacggtc tggccggggc
     7561 tttcctagtt atcggcctgt ttttcggtct ggctgaactt tgggccggac ggatgcgttt
     7621 tgtggggctg agtcataagg agcgacggga gtatgccgat cgctataagc gcgataataa
     7681 acggccagcg ccattttcgg tgctggcctc gctggattcg cccgggcgga tggtcagggc
     7741 acgtcgacat gtctggaact acgcgtactg gctgaccggc gcggccggtc tggcccttct
     7801 gggggggctg gttgtcgccg tgtttatcga gagctgggga gaaaccgtcc tgcgtccgga
     7861 cctctacgca aacgcagatt attacctgct gcagccggct aacctgttcg ggattgcctg
     7921 gtgcgcgggc gggctggctg tcgggattgt gttggcgcgg ctgctggcgt tcctgctgct
     7981 tgacggtcat tttgcggctg ctgacgaggc ggtgaacgcg aaactgaatc aggagagccg
     8041 gcgcagcagc cagcggacag gagagatgac cgacgtgcgc cacctgcatt tcggggagcc
     8101 ggtaccggtt aatgcgctgg ccgatttttc ctcggagcag gcccggcagg agcaggcggt
     8161 gtttctgggt aaggatgagc agggcaagcc ggtgctggtt ccgcgtgata cctggcgcaa
     8221 gaccaacatt cagatcctcg gcctgcccgg gagcggcaag agcgtgatgg ccaccaacgc
     8281 gctcattcgc tgcgtacgcg atttcgggga cgcggtggtg tacttcgacc ccaaagggga
     8341 cgcatgggcg ccgcacgtct tccgggcgca ctgtccggac ttcaccctgc tggatctgcg
     8401 gccggggaaa ccggcgcagc tgaatctgtt ccgcgatctc gaccagtacg cgctgaaaaa
     8461 cctgctgatt gccgggctta acctgggcga gaccggcgac gtggcggacc actaccgcat
     8521 cagcgagcag aaagcggcga agctgattgc ggagcagttt ccgcaggggg caaacattca
     8581 gcaggtgctg gcggcggcgt acgcgctgcc ggaggccctg aaaaaggacg tgaaggggtt
     8641 gatcactagg ctggaaaacg tcgctgacct gagcgtcctg cagacggaca gtggtatcga
     8701 tgtggcgggg attatcaacg gcggcggctg cctgtacgtt atcggctcga tggacgacga
     8761 ggcggtgatc cgtgtgcaaa aaatgctgtt tgcccgctgc gcgcaaatca tcattgcgcg
     8821 ggacgagttc cgggagtggc cacacgtcag cgtgatgctc gatgaaatta aatatctgct
     8881 gtcgaagtat gtactgaacg cactggggac gctgcgcagc cgggactgca atctgttgct
     8941 cgcgcaccag tcgctgggcg atttcgggca gtgcgggcag gatctgcccg ctgaatttgt
     9001 gaaaaccacg gtactggata acacgccgat ccgctggttt taccgggcgg ccagcctgga
     9061 gtccgcgcag tgggcggcgg ggcagaccgg ggaaatcagg gtggacgtgg agcgccgccg
     9121 tgccagccgg gaggcgggga acgtggagca tatcagcggg gacagcttca tccagaagga
     9181 ggcgcggccg ctgtttgacg tgaacaccct gcaacacctg ccggacgggt tcgcggtcat
     9241 gaccgggc
//
