LOCUS       NZ_CP070566             5598 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KP51248 plasmid p51248_2, complete
            sequence.
ACCESSION   NZ_CP070566
VERSION     NZ_CP070566.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933289
            Assembly: GCF_016939695.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939695.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 02:48:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,506
            CDSs (total)                      :: 5,385
            Genes (coding)                    :: 5,244
            CDSs (with protein)               :: 5,244
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 67 of 141
            Pseudo Genes (incomplete)         :: 67 of 141
            Pseudo Genes (internal stop)      :: 26 of 141
            Pseudo Genes (multiple problems)  :: 15 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070566.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP51248"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p51248_2"
                     /geo_loc_name="Czech Republic: Ostrava"
                     /lat_lon="49.84 N 18.28 E"
                     /collection_date="2019-06-14"
                     /collected_by="Zdravotn stav se sdlem v Ostrav"
     gene            join(5286..5598,1..233)
                     /locus_tag="JW300_RS00620"
                     /old_locus_tag="JW300_00620"
     CDS             join(5286..5598,1..233)
                     /locus_tag="JW300_RS00620"
                     /old_locus_tag="JW300_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433973.1"
                     /translation="MFFKRYLTEKEIYKVALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            299..649
                     /locus_tag="JW300_RS00625"
                     /old_locus_tag="JW300_00625"
     CDS             299..649
                     /locus_tag="JW300_RS00625"
                     /old_locus_tag="JW300_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(719..985)
                     /locus_tag="JW300_RS00630"
                     /old_locus_tag="JW300_00630"
     CDS             complement(719..985)
                     /locus_tag="JW300_RS00630"
                     /old_locus_tag="JW300_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(969..1193)
                     /locus_tag="JW300_RS00635"
                     /old_locus_tag="JW300_00635"
     CDS             complement(969..1193)
                     /locus_tag="JW300_RS00635"
                     /old_locus_tag="JW300_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1356..1484
                     /locus_tag="JW300_RS27630"
     CDS             1356..1484
                     /locus_tag="JW300_RS27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(1860..2072)
                     /locus_tag="JW300_RS00640"
                     /old_locus_tag="JW300_00640"
     CDS             complement(1860..2072)
                     /locus_tag="JW300_RS00640"
                     /old_locus_tag="JW300_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2079..2588)
                     /locus_tag="JW300_RS00645"
                     /old_locus_tag="JW300_00645"
     CDS             complement(2079..2588)
                     /locus_tag="JW300_RS00645"
                     /old_locus_tag="JW300_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<2623..3327)
                     /locus_tag="JW300_RS27745"
                     /old_locus_tag="JW300_00650"
                     /pseudo=""
     CDS             complement(<2623..3327)
                     /locus_tag="JW300_RS27745"
                     /old_locus_tag="JW300_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3266..3610)
                     /locus_tag="JW300_RS00655"
                     /old_locus_tag="JW300_00655"
     CDS             complement(3266..3610)
                     /locus_tag="JW300_RS00655"
                     /old_locus_tag="JW300_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004197764.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3600..3776)
                     /locus_tag="JW300_RS00660"
                     /old_locus_tag="JW300_00660"
     CDS             complement(3600..3776)
                     /locus_tag="JW300_RS00660"
                     /old_locus_tag="JW300_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165764108.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSV
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4416..4574
                     /locus_tag="JW300_RS27750"
     CDS             4416..4574
                     /locus_tag="JW300_RS27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4934..5260
                     /locus_tag="JW300_RS00665"
                     /old_locus_tag="JW300_00665"
     CDS             4934..5260
                     /locus_tag="JW300_RS00665"
                     /old_locus_tag="JW300_00665"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197760.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINEMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          complement(3863..3943)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.476"
                     /coverage="98.0"
                     /evalue="3.1e-22"
     Typing          4128..4525
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.801"
                     /coverage="91.0"
                     /evalue="6.52e-141"
     Typing          complement(1745..3276)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaacccatg ggggaagaaa aatgataatc cactaatatt tataatgagc attgaagaaa
       61 gtataaaaga aacccttaac tcaggttata tgttatatag agtagctccc ataactgagg
      121 gtgaagcagc tgctttcatg gatgttctta aacaattatt attatttgag gataattttc
      181 gcctttttgg tagggacttg gtttataaaa tagaaaaatt aaaaatcaga taacaaagta
      241 tggtgaacga aaaatgaaag agaggatagt gaaatgtgta ataccaagat tctcaactat
      301 gtattggtta attgcgttcc tagcatttat attccccggt cgatcttttg ctgacggtta
      361 caccgcttac taccgatgta atggtactca ggaagcatct cttactgtta aagataacaa
      421 actaagtatg gttgtaggtc tttctaagtt taataggcaa ctggatactt atagattcaa
      481 agaaaaggga atcgaagtag taatgcagag agctgcttct gatgataact ctgaggccat
      541 aatgtctttt attatgcctg acgttaacaa tatgacctta ttagttaaaa ataacggcta
      601 cattatcgtt aagacaacct gctggttaac taaaaccgac aaacagtaga acacgtagtt
      661 agtcgagtat ttaccataat acctgttgtc ggcatcaaaa aagcccgcat agcgggcttc
      721 agtcgtaaac ttcgcggcga tgtccaatcg tggcggcaag gataacaagc tcgtcatctc
      781 ggatctcaca cagaacgcga taatctccca cccgatagcg ccagaactcg cccagctcac
      841 ctttgagcgg cttccctgac tggcgaggat cggcagcaac tgcaatgcgt aaactcataa
      901 aatccacaat ccgtcgtgcg ttctgtttgt ccatcttgcg taacgatttg agcgcccgat
      961 cggaatagtt aatcgtccag accaagtgac ttcctcacgt cttcagacga atgcactgct
     1021 tcatcaccac ggcgaacgcg tgcagcagtt tctgctgaaa gataataatc ctccaggtct
     1081 tccagatgcg ccagtattgc ttcccgcgca taaaacgtct ttgttctgcc ggtttgcttc
     1141 gccagcgagt ccagacggga ctcaatctca tcagataacc ggatagcaag catataaacc
     1201 tcccgaacac catttgctat acatgtatat caacagtacc acaatcaacc taaaagaaca
     1261 aaaaataatc ttgttagccc ctcttgataa tccgtaacgc ggtcaatata tctagcttga
     1321 gggctttcct cttaactatt gaaaggataa cgattatggg tcttaagccg ggtccgaaac
     1381 gaattgccga ttcaacaggg gaacctgata aacgccagcg tgacaataaa aaaacgccgg
     1441 gaaatactga caaattgaaa cccagcaaat cttcaaaaaa ataagtcagt agttaaaagc
     1501 cctccggggc ttttttgtat ctgccgttca cgctgattaa acataacacc cgttgcccgc
     1561 accgccccgg acgggttcgc cgcgaacggg gtggcagtca ggtaaaaccg gcaacaaacc
     1621 acccgataaa ccgccccgtt cgcgacagat aaattttacc tggctgcata gccatgcagc
     1681 caggtgaatg acatccggtt tcagcgtaaa tgcccgatga gaaccctgtt ttcctgcatt
     1741 tcagcccagc gacggaccgt catagcttcg ctgccgggag cgcgcttccg tcagttcctt
     1801 ttcgtgctgc cgttgctcgt gcgcccgttc tgcggccacg gccatctcat gacgctgcac
     1861 taccggctca aacgctgcag ttgcccgctc aagcgttcca cctgctgact caagcgcgtc
     1921 acgcgctcgc tcagcgccgc gttctcccgt tgcataagcc ctgacatttt ccgccattcc
     1981 ccgaaggcgc tctcccactc gtccagcctt ttcgagtagt cctgctgtag ctgctctaat
     2041 gcgctcagca actgtttttc cagctcggtc atcggcactt actccttcac cagtctcagc
     2101 gcgttctgct tcgaccgccc gttcccgacc gtccagctct gcgtcctgtc tacggccgtc
     2161 cccttcggca gcaccagaaa acgtccgttg ctgtcttcca ggtacgtcac gccccaggtc
     2221 ttcgcgttca gcttcgacag cgccgcgttc tgccggtcga tttcggccag atttgacgct
     2281 atcaggctcc cctgatacca caacaccccg ctcaggctgg cgaacagcag tcctgacacc
     2341 atcgtgatgg tcagccatgt ccgcccgacc atacgcatca cgctcaggcg gttcgactgc
     2401 atggccgcgc tcataccctg ctcgtgggcg cggatggcgg cgctgattct cttcccgctt
     2461 tcgctcagtt ccgctctgac agacttctcg tgctcgctga atgcggcttt cagcatttcg
     2521 ccggtactct gctgctgcgc tttcgatttc tgctctaagt ccttcgccag cgttaaaaga
     2581 ctgttcatag atggctccct taagtcggat gtttcgccct ccgtccgggt cggcaatgct
     2641 gatgctgctc tttgtggtgc gcacgacctc aaaacctgcg ctttccagcg cctcagtgac
     2701 gtcctgacgc gttttaagct cccctgacga ggcgagggcc agtaagcccc gcgtaatagc
     2761 ctctgcggct tcctgcttcg ttttgggtaa cgaagatggc gtcaccagcg cgcgccggtt
     2821 ctccggtgcg ttcgggtcgt gcagccccag tcgcccgttc accacggtct gccacgcatc
     2881 gatgcgcgga cggtctgccc ggtcgtagta cggctggaga cgcctgccgg tcagcagttc
     2941 cgtgttcggt accagaaaat tcagttccag ccgccccttg tcccggtgtt caacccacag
     3001 cacgctgtac tggtctttat cgagtccggg catcagaacc cgctcgaagc tcgccatcag
     3061 cttttcacgc tggccgggcg gtaaatcctg ttcagcaaaa gacaggacgc cggaggtgta
     3121 ctttttggcg tagggcgagg cgtcgataag ctcccggacc tcctccggct ttccctgcag
     3181 gacgatggcc ccgtcgcgct ggcggtcttt ccccagcaga taatccaccg gcccggcacc
     3241 gccgcctcgc cctcgcggat gaaacttaac gatcatcgtc tgcccccttt tccagcacgg
     3301 catgccgcag ccgctcgagt ccggcatcga tggccatcag cgcggccacc acctgaaccc
     3361 ggtcagcgcc tgaccactgg ccgccgttaa ttttccgggc gatctggttg aggttgttcc
     3421 ccatcccggc aagctggcgc agcaaaaccg gatcgatgga cggcagccgc aacgaccgcg
     3481 ccgggcgcgt atcgagacag gtttcccgca tccacaccgc cagttgcctt ccattgcagc
     3541 gctccagcag ctgctggtac tccccatcgg tcacccggat acctaaaaaa cgggtacgct
     3601 taaccgccat cctctcctcc gatatccgca aaatgtgacc ggactgaacg ctcaagctct
     3661 gccatctgct cccggcggta atcttcctcc tgtgcctgac gcatcagatt tgccatcact
     3721 gccggatgcc aggatctttt ttttacgacg agcggcttct tcttcggctt ctgcattatc
     3781 cctccttcgc aaacgccgga cggcacggaa atgttttccg ggacgcgccg gcgttggaca
     3841 agcgccagcg cgtcagtggt gttagcgggt ttcggggcgc agccctgaac cagtcacagc
     3901 gtttacgctg tgtatactgg cttactatgt tggcatgaga gcgagattca tgaaagtgcc
     3961 tcatgtggca ggtgtgatat gtgactcagg agcgtccgca ataccatgca cggacagatg
     4021 aaatgacgcc tcctcgctca ctcggtcgct gcgctcctgc cgttcggctg cggcgggcgt
     4081 gaccggctca cggataacgg gataaagcaa aggcgctcaa atcagaaagt gccggagaaa
     4141 aggatacggc gtggccgttt ttccataggc tccgcctccc ctgacgagca tcacagaaat
     4201 ggacgctcaa gtcagaggag gacgaaaccc gacaggactt aaagatgccg gtgatttctc
     4261 tccggcggct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccga atacctgttc
     4321 cgcctttttc cctttcggga agcgtggcgc tttctcatag ctcacgctgc tggtatctcg
     4381 gctcggttta ggtcgttcgc tccaagctgg gctgtatgca cgaacccccc gttcagcccg
     4441 actgctgcgc cctttccggt aactatcatc ttgagtccaa cccggacgga cacgcaaaaa
     4501 cgccactggc agcagccatt ggtaactgga tatatacgag agagatattg agaggtcttg
     4561 aagtgggtgc ctaactgcgg ctacactgaa agaacatttt tggtatctgc ggtctcgtat
     4621 acccagttac ccggttaagc agttccccaa ctgacttaac cttcgatcaa accgcctccc
     4681 caggcggttt tttcgtttac agtgcaagag attacgcgca gaaaaaaagg atctcaagaa
     4741 gatcctttaa atgatcgtca gttctgttat ccccggagct ccatccatcc cacggaaaaa
     4801 cacccgccac tgatgccctc agcgcactgc acatcatgta caaaccactc cctccctcct
     4861 tttttattgc gtttcttaat gcgcaatggt agctttgtgc gcaacaaaaa acgcaatagc
     4921 gagggggggg gtaatgagag aaagaacaaa tatcatgcta gatataggat taaagcaatc
     4981 cttacaacgg ctttcgaata aaaccaaaat gagtctaagt gatcttatta accagagatt
     5041 atcaggttca ctttcagttg aagaacgcat tggcgaaaag cctgaatgga tgagaaacct
     5101 tgatccctat ctcgccaatc acttttcaaa tattgagtta ccgctccctt caccattaaa
     5161 agaataccaa gaaaaaatgg atgagataga ttcaattatt aatgaaatga tggttctaaa
     5221 ggaatatttc agagctaagt tttacgacga aataaaatag gcacgctata cggaggcgga
     5281 gaattatgtt ttttaaacgg tatttaacgg agaaggaaat atacaaggta gcactcccaa
     5341 ttgttaggct tcaagggaga gttaacactt cattacgttt tttatccaaa tactcagatg
     5401 taaacaaaaa tggattaatt gcattacatc ttaatttatt acattttatc tcatacaaca
     5461 tacacggtgt agagtctctg aaaatcactc aagcaggcat aaacaaagct gtacaacaaa
     5521 gccctcacgc agatgaaata aaaaacattg cgcaggcaat aggacgcaaa ggacctgagt
     5581 acatcgaagg attaatta
//
