LOCUS       NZ_CP070563            13637 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KP51069 plasmid p51069_ColRNAI,
            complete sequence.
ACCESSION   NZ_CP070563
VERSION     NZ_CP070563.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933286
            Assembly: GCF_016939675.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 13637)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13637)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939675.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 02:57:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,677
            CDSs (total)                      :: 5,553
            Genes (coding)                    :: 5,408
            CDSs (with protein)               :: 5,408
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 78 of 145
            Pseudo Genes (incomplete)         :: 82 of 145
            Pseudo Genes (internal stop)      :: 17 of 145
            Pseudo Genes (multiple problems)  :: 27 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070563.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13637
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP51069"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p51069_ColRNAI"
                     /geo_loc_name="Czech Republic: Tinec"
                     /lat_lon="49.67 N 18.69 E"
                     /collection_date="2019-05-27"
                     /collected_by="Nemocnice Tinec"
     gene            complement(join(13291..13637,1..208))
                     /gene="aac(6')-Ib"
                     /locus_tag="JW299_RS01745"
                     /old_locus_tag="JW299_01745"
     CDS             complement(join(13291..13637,1..208))
                     /gene="aac(6')-Ib"
                     /locus_tag="JW299_RS01745"
                     /old_locus_tag="JW299_01745"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /protein_id="WP_063840280.1"
                     /translation="MTNSNDSVTLRLMTEHDLAMLYEWLNRSHIVEWWGGEEARPTLAD
                     VQEQYLPSVLAQESVTPYIAMLNGEPIGYAQSYVALGSGDGWWEEETDPGVRGIDQLLA
                     NASQLGKGLGTKLVRALVELLFNDPEVTKIQTDPSPSNLRAIRCYEKAGFERQGTVTTP
                     DGPAVYMVQTRQAFERTRSVA"
                     /name="aac(6')-Ib"
     gene            complement(442..999)
                     /locus_tag="JW299_RS01750"
                     /old_locus_tag="JW299_01750"
     CDS             complement(442..999)
                     /locus_tag="JW299_RS01750"
                     /old_locus_tag="JW299_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001217881.1"
                     /translation="MRIFGYARVSTSQQSLDIQIRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            1161..>4134
                     /locus_tag="JW299_RS01755"
                     /old_locus_tag="JW299_01755"
                     /pseudo=""
     CDS             1161..>4134
                     /locus_tag="JW299_RS01755"
                     /old_locus_tag="JW299_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /name="Tn3-like element Tn3 family transposase"
     gene            <4202..4492
                     /locus_tag="JW299_RS01760"
                     /old_locus_tag="JW299_01760"
                     /pseudo=""
     CDS             <4202..4492
                     /locus_tag="JW299_RS01760"
                     /old_locus_tag="JW299_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /name="Exc2 family lipoprotein"
     gene            4798..5061
                     /locus_tag="JW299_RS01765"
                     /old_locus_tag="JW299_01765"
     CDS             4798..5061
                     /locus_tag="JW299_RS01765"
                     /old_locus_tag="JW299_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            5051..5350
                     /locus_tag="JW299_RS01770"
                     /old_locus_tag="JW299_01770"
     CDS             5051..5350
                     /locus_tag="JW299_RS01770"
                     /old_locus_tag="JW299_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5412..5789)
                     /locus_tag="JW299_RS01775"
                     /old_locus_tag="JW299_01775"
     CDS             complement(5412..5789)
                     /locus_tag="JW299_RS01775"
                     /old_locus_tag="JW299_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            5858..6037
                     /locus_tag="JW299_RS01780"
                     /old_locus_tag="JW299_01780"
     CDS             5858..6037
                     /locus_tag="JW299_RS01780"
                     /old_locus_tag="JW299_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6065..6595)
                     /locus_tag="JW299_RS01785"
                     /old_locus_tag="JW299_01785"
     CDS             complement(6065..6595)
                     /locus_tag="JW299_RS01785"
                     /old_locus_tag="JW299_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6602..7333)
                     /locus_tag="JW299_RS01790"
                     /old_locus_tag="JW299_01790"
     CDS             complement(6602..7333)
                     /locus_tag="JW299_RS01790"
                     /old_locus_tag="JW299_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(7333..9297)
                     /locus_tag="JW299_RS01795"
                     /old_locus_tag="JW299_01795"
     CDS             complement(7333..9297)
                     /locus_tag="JW299_RS01795"
                     /old_locus_tag="JW299_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(10778..10927)
                     /locus_tag="JW299_RS01800"
                     /old_locus_tag="JW299_01800"
     CDS             complement(10778..10927)
                     /locus_tag="JW299_RS01800"
                     /old_locus_tag="JW299_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(11012..11269)
                     /locus_tag="JW299_RS01805"
                     /old_locus_tag="JW299_01805"
     CDS             complement(11012..11269)
                     /locus_tag="JW299_RS01805"
                     /old_locus_tag="JW299_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(11279..>11863)
                     /locus_tag="JW299_RS01810"
                     /old_locus_tag="JW299_01810"
                     /pseudo=""
     CDS             complement(11279..>11863)
                     /locus_tag="JW299_RS01810"
                     /old_locus_tag="JW299_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /GO_function="GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]; GO:0043022 - ribosome binding
                     [Evidence IEA]; GO:0003723 - RNA binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3/pyocin S6 family cytotoxin"
                     /name="colicin E3/pyocin S6 family cytotoxin"
     gene            complement(12011..>12181)
                     /locus_tag="JW299_RS01815"
                     /old_locus_tag="JW299_01815"
                     /pseudo=""
     CDS             complement(12011..>12181)
                     /locus_tag="JW299_RS01815"
                     /old_locus_tag="JW299_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /name="serine hydrolase"
     gene            complement(12236..12940)
                     /locus_tag="JW299_RS01820"
                     /old_locus_tag="JW299_01820"
     CDS             complement(12236..12940)
                     /locus_tag="JW299_RS01820"
                     /old_locus_tag="JW299_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_004217321.1"
                     /translation="MNPFKGRHFQRDIIRWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            complement(<13006..13221)
                     /locus_tag="JW299_RS01825"
                     /old_locus_tag="JW299_01825"
                     /pseudo=""
     CDS             complement(<13006..13221)
                     /locus_tag="JW299_RS01825"
                     /old_locus_tag="JW299_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001424636.1"
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing protein"
                     /name="nucleotidyltransferase domain-containing protein"
     ARG             complement(12953..13209)
                     /name="aadA15"
                     /gene="aadA15"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.14"
                     /coverage="32.32"
     ARG             complement(13292..13635)
                     /name="AAC(6')-Ib'"
                     /gene="AAC(6')-Ib'"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.12"
                     /coverage="61.96"
     ARG             complement(3..259)
                     /name="AAC(6')-Ib7"
                     /gene="AAC(6')-Ib7"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="98.75"
                     /coverage="32.95"
     Typing          10097..10675
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6606..7333)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.34e-150"
ORIGIN
        1 ctccattcag cattgcaatg tatggagtga cggactcttg cgctaaaacg cttggcaagt
       61 actgttcctg tacgtcagca agtgtcgggc gtgcttcttc tccgccccac cactcgacga
      121 tatgagatcg atttagccac tcatagagca tcgcaaggtc atgctcagtc atgaggcgca
      181 gtgtgacgga atcgttgctg ttggtcacga tgctgtactt tgtgatgcct aactttgttt
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacttt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
      421 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaagaattt tataaaccgt
      481 ggagcgggca atactgagct gatgagcaat ttccgttgca ccagtgccct tctgatgaag
      541 cgtcagcacg acgttcctgt ccacggtacg cctgcggcca aatttgattc ctttcagctt
      601 tgcttcctgt cggccctcat tcgtgcgctc taggatcctc cggcgttcag cctgtgccac
      661 agccgacagg atggtgacca ccatttgccc catatcaccg tcggtactga tcccgtcgtc
      721 aataaaccga accgctacac cctgagcatc aaactctttt atcagttgga tcatgtcggc
      781 ggtgtcgcgg ccaagacggt cgagcttctt caccagaatg acatcacctt cctccacctt
      841 catcctcagc aaatccagcc cttcccgatc tgttgaactg ccggatgcct tgtcggtaaa
      901 gatgcggtta gcttttaccc ctgcatcttt gagcgctctg atctgaatat cgagggactg
      961 ctggctggtt gagacccgcg cataaccaaa aattcgcata aaaatgtacc ttaaatcgaa
     1021 tatcagacac gatgtgtcta ttatgccaaa atgacgattt aatggacact caaacgaagc
     1081 cgttttacta tgtctgataa tttataatat ttcgaacggt tgcagttgtg ttaaaaaagc
     1141 cgtcaggcag ggaggccgat atgcccgttg attttttgac cactgagcag gttgagagtt
     1201 atggcaggtt cactggcgaa cccgatgaac ttcagctggc gcgttatttt catcttgatg
     1261 aagcggataa agaatttatc gggaaaagcc ggggtgatca caatcgactt ggtattgccc
     1321 tgcaaatcgg gtgtgtgcgt tttctgggca cttttcttac tgacatgaat catattcctt
     1381 ccggcgtccg gcattttacc gccagacagc tcgggattcg tgatatcacc gttcttgcag
     1441 aatacggtca gagggaaaat acccgccgtg agcatgcagc gctgatacgt cagcactatc
     1501 agtatcgtga atttgcctgg ccctggacat ttcgccttac ccgtctttta tatacccgga
     1561 gctggataag caacgaacgt cctggcctgc ttttcgacct ggcgacaggg tggcttatgc
     1621 aacatcgtat tattctcccc ggagccacca cgctgacccg gttgatttca gaggtaaggg
     1681 aaaaggcgac gttgcgcctg tggaacaaac tggcactgat accgtcagcc gaacagcgtt
     1741 cacagctgga gatgctgctg gggccaactg attgcagccg cctgtcttta ctggaatcac
     1801 tgaaaaaagg ccctgtgacc atcagtggtc cggcgtttaa tgaagcaatt gaacgctgga
     1861 aaactctgaa cgattttggc ctgcatgctg aaaacctgag tacactcccg gctgtgcgcc
     1921 tgaaaaatct cgcacgttat gctggtatga cttcggtgtt caatattgcc aggatgtcac
     1981 cgcagaaaag gatggcggtt ctggttgcct ttgtccttgc atgggaaacg ctggcgctgg
     2041 atgatgcact ggacgttctg gacgccatgc tggccgttat catccgtgac gccagaaaga
     2101 ttgggcagaa aaaaacggct ccgctcgctg aaggatctgg ataaatctgc attggcgctc
     2161 gccagcgcat gttcgtactt gctgaaagaa gaaacaccgg acgaatcgat tcgtgctgag
     2221 gtgttcagct acatccctag gcaaaagctg gctgaaatca tcacgcttgt ccgtgaaatt
     2281 gcccggccct cagacgataa ttttcatgac gaaatggtgg agcagtacgg gcgcgttcgt
     2341 cgtttcctgc cccatctgct gaataccgtt aaattttcat ccgcacctgc cggggttacc
     2401 actctgaatg cctgtgacta cctcagccgg gagttcagct cacggcggca gttttttgac
     2461 gacgcaccaa cggaaatcat cagtcagtca tggaaacggc tggtgattaa caaggaaaaa
     2521 catatcaccc gaaggggata cacgctctgc tttctcagta aactgcagga tagtctgaga
     2581 cggagggatg tctacgttac cggcagtaac cggtggggag atcctcgtgc aagattacta
     2641 cagggtgctg actggcaggc aaatcggatt aaggtttatc gttctttggg gcacccgaca
     2701 gacccgcagg aagcaataaa atctctgggc catcagcttg atagtcgtta cagacaggtt
     2761 gctgcacgtc ttggcgaaaa tgaggctgtc gaactcgatg tttctggccc gaagccccgg
     2821 ttgacaattt ctcccctcgc cagtcttgat gagccggaca gtctgaaacg actgagcaaa
     2881 atgatcagtg atctgctccc tccggtggat ttaacggagt tgctgctcga aattaacgcc
     2941 cataccggat ttgctgatga gtttttccat gccagtgaag ccagtgccag agttgatgat
     3001 ctgcccgtca gcatcagcgc cgtgctgatg gctgaagcct gcaatatcgg tctggaacca
     3061 ctgatcagat caaatgttcc tgcactgacc cgacaccggc tgaactggac aaaagcgaac
     3121 tatctgcggg ctgaaactat caccagcgct aatgccagac tggttgattt tcaggcaacg
     3181 ctgccactgg cacagatatg gggtggagga gaagtggcat ctgcagatgg aatgcgcttt
     3241 gttacgccag tcagaacaat caatgccgga ccgaaccgca aatactttgg taataacaga
     3301 gggatcacct ggtacaactt tgtgtccgat cagtattccg gctttcatgg catcgttata
     3361 ccggggacgc tgagggactc tatctttgtg ctggaaggcc ttctggaaca ggagaccggg
     3421 ctgaatccaa ccgaaattat gaccgatacg gcaggtgcca gcgatcttgt ctttggcctt
     3481 ttctggctgc tgggatacca gttttctcca cgcctggctg atgccggtgc ttcggttttc
     3541 tggcgaatgg accatgatgc cgactatggc gtgctgaatg atattgccag agggcaatca
     3601 gatccccgaa aaatagtcct tcagtgggac gaaatgatcc ggaccgcagg ctccctgaag
     3661 ctgggcaaag tacaggcctc agtgctggtc cgttcattgc tgaaaagtga acgtccctcc
     3721 ggactgactc aggcaatcat tgaagtgggg cgcatcaaca aaacgctgta tctgcttaat
     3781 tatattgatg atgaagatta ccgccggcgc attctgaccc agcttaatcg gggagaaagc
     3841 cgtcatgcag ttgccagagc catctgtcac ggtcaaaaag gtgagataag aaaacgatat
     3901 accgacggtc aggaagatca gttgggagct ctggggctgg tcactaacgc cgtcgtgtta
     3961 tggaacacta tttatatgca ggcagctctg gatcatctcc gggcgcaggg tgaaacactg
     4021 aatgatgaag atatcgcacg cctctccccg ctttgccacg gacatatcaa tatgctcggc
     4081 cattattcct tcacgctggc agaactggtg accaaaggtc atctgagacc attaaaagag
     4141 gcgtcagagg cagaaaacgt tgcttaacgt gagttttcgt tccactgagc gtcagacccc
     4201 ttttgatccc aatttttcca cccagaaagc tgacacaaca cgaatgatgg tgcctttttt
     4261 ccggcagttc tgggatatgg gcgcaaagga taaagcgacc ggaaaatcac ggagtgatgt
     4321 gcagcaacgc attcagcagt ttcacagtca ggaattttta aactcactcc aaggcacaac
     4381 acaatttgcg ggtaccgatt accgcagcaa agacctcaca ccgaaaaaat caaggctact
     4441 ggctgacacc atttcagctg tttatctcga tggctatgaa ggcagacagt aagtggattt
     4501 accataacgc gtcattacgc gcaccgctca gacgggctca gggcgtttcc ggcggctacg
     4561 ggataaaaca ggcaacaacc cccccggaaa actgccggaa acgcgcccga taaattttat
     4621 cctaatgcat gcctatgcac gcaactgcat ggcatatgtt ttgacgtaaa atggatgccg
     4681 taagcccttt tttaccgact ttctgcttca tcagcctgac tgacacgacc atattctttc
     4741 agaccgcccc ctgctaaact tccttcatat tgccgcatta acccgggagg ctccgcaatg
     4801 agcaacacta cacactacga aaacgccaac tttcttcggg aactggctga aagcctgccc
     4861 cgcatactgc cggaaggtgg cccggacaaa gctgcgctgt tgcagcgact ggctaacgaa
     4921 gaactggccc aggcagagta tgaagatcag gttcgcgcca aagtgaccgc cgcccgtgct
     4981 gacacgcgtc cgggtatgac cacggaacaa ctccgccagc gcctgcatgg ccgttatcag
     5041 gaactgcgcg atgccgtatg acgtggaatg gaagcaggga gccatcgagg atgtaacgac
     5101 gctgtttgac tatatcgcgg aaaattcatc cctgtgggat gcgcagaacg ttaccgaccg
     5161 ggttctcgct gcggcggaca aactggccga cttcccgcga ctctatgaag tggactcacg
     5221 ctatggcgag gacgttcgcc ggatcagtct gatgggacag catgtcctct atgatgttga
     5281 tgaccagacc agaaaagtcc gggttctggc cgtggtcggc cagcgccaga atccccatat
     5341 tgttctctaa taaccggtga cggggacgca attcattgcg tggccgtcgc cggttggaca
     5401 agcacagcgc gtcagcgttt tccccacggc caccaccggc gcggtgttcc ggccggggtc
     5461 tctgctgtct ccggtttgtg ttccagcagt ctcatggact gcatttcctg ccgtagcccg
     5521 gccacctcct ccctgagtgc ttttatctcc gcgatcaacg cgtcggaggc cattttttca
     5581 gtctcacccg acagtctcaa ctcgtcccgc tcgtgagtct catcctgtct taactcaccg
     5641 tacacccgga taagctcgct ggtatccacc acccggccgc catcagcttc cgtacggtat
     5701 gatacgcggc ctttcgccat atcccgatac agggaacttc gggctttacc cgtcagttcc
     5761 gtcgcctgtc tcaccgtcac ccacgccatt gtctcacccc gctatttttg agacagtata
     5821 cctgacaggc gcacaccatg cccggaagga aagaatcatg acaaaacagg aaaaaacagc
     5881 cctcaatatg gctaaattta ttaaggagca gtcgctgcta ttgctcgata aactgaatga
     5941 gctggatatg gataccccgg ccgacatgtg cgagcggcta cacgaggatg cggaaacact
     6001 ctggcaggtg atgcgggata agctcgggga ggaataacag caccggcatg gccggtgctg
     6061 gcatttattt tttgccgaac ggccaccacc ggcgggcagg tttgtcctcc ggcagttcag
     6121 gtaccggcac gttaaaatag cggctgttat attcccgcat cagctccgct ttcagcttct
     6181 cgttcaccgc gctttccccg tgccgctcac tcaccacgtc aatcatcgcg ttaatctgct
     6241 ccggtgagca cttcccggca tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca
     6301 ccttgcgata gaggtaatca cacagcttca ttcctgtggg tttgttccgt tcccattgcg
     6361 gatttttacc agtgaaaaag tacgtggctt tttcgtcgct gttgagccgc tcgtcataca
     6421 tcccgatgga tttcatcatc gccagatgct gggcccggtc gacaaaacag aaaacatacg
     6481 ccccggtctg ccaggcgagc accaccatat cttccagatg ctcgctggtc ggcatgatgc
     6541 agcgctcgcc gttccggtcc tccgcgtctt caataaatac catgtacagc gccataatcc
     6601 ctcactccgt tgtcataaat ttaaagcacg caaacaaccg ttcacgatcc agctctgccg
     6661 ttttgcgggc ctcgccggtc tgtgggttca cgatttcatc caccgacagg attgcccgct
     6721 gcaccctgac ctgcgtcgcc cggtccgggc agatgtacca gatttcatcg atgccgttag
     6781 ctttgcggtt gaacagatac ccggacacca cttcctgata ccggcgacgg ctcttgacgg
     6841 tgcgctccac ctcaaacgcc accgttttac cgtccagctc cgccagggcg tccggcactt
     6901 tcatgccttt aagactcaga cggtgcagaa tgcgccagcc ggtggcccct ctggcttcca
     6961 tatcaaggcg cagggattgc acggccagcc ggtgcggcag atgtgctgcc gacacccttg
     7021 acggctgaaa cggaataaca tccgccagcg gttcgtcctc gtcgaacgag aacgccacgc
     7081 catgcggcgt cagcccccac acgctgacct gacggccgtc caccggatat ttggtccgga
     7141 ccagcagccc tttcgccacc aggctgtcga ggaagcggta gaccggctgc accgccgcaa
     7201 tccccagccg ccgcgcaacc agctccgccg tggaacacag ctcttcccgc aaccagcgca
     7261 gggtcagccg ggttttctcc gcgttccgcg cctgtcgttt cgcgtggctg acgttgtaca
     7321 gctccagcgc catcagtaaa tccccgcaaa atcatcatcc gctttcggcc cgccgccgtc
     7381 ggccgggggc cggaactccg ccaggggatc ggttttcgcc agcaccggga aacgctgcaa
     7441 cgggatttcc cggcgctcaa cccgcagcgg actgctgaac gccatccggg ccacacccag
     7501 cccggtcatg accgcgaacc cgtccggcag gtgttgcagg gtgttcacgt caaacagcgg
     7561 ccgcgcctcc ttctggatga agctgtcccc gctgatatgc tccacgttcc ccgcctcccg
     7621 gctggcacgg cggcgctcca cgtccaccct gatttccccg gtctgccccg ccgcccactg
     7681 cgcggactcc aggctggccg cccggtaaaa ccagcggatc ggcgtgttat ccagtaccgt
     7741 ggttttcaca aattcagcgg gcagatcctg cccgcactgc ccgaaatcgc ccagcgactg
     7801 gtgcgcgagc aacagattgc agtcccggct gcgcagcgtc cccagtgcgt tcagtacata
     7861 cttcgacagc agatatttaa tttcatcgag catcacgctg acgtgtggcc actcccggaa
     7921 ctcgtcccgc gcaatgatga tttgcgcgca gcgggcaaac agcatttttt gcacacggat
     7981 caccgcctcg tcgtccatcg agccgataac gtacaggcag ccgccgccgt tgataatccc
     8041 cgccacatcg atgccactgt ccgtctgcag gacgctcagg tcagcgacgt tttccagcct
     8101 agtgatcaac cccttcacgt cctttttcag ggcctccggc agcgcgtacg ccgccgccag
     8161 cacctgctga atgtttgccc cctgcggaaa ctgctccgca atcagcttcg ccgctttctg
     8221 ctcgctgatg cggtagtggt ccgccacgtc gccggtctcg cccaggttaa gcccggcaat
     8281 cagcaggttt ttcagcgcgt actggtcgag atcgcggaac agattcagct gcgccggttt
     8341 ccccggccgc agatccagca gggtgaagtc cggacagtgc gcccggaaga cgtgcggcgc
     8401 ccatgcgtcc cctttggggt cgaagtacac caccgcgtcc ccgaaatcgc gtacgcagcg
     8461 aatgagcgcg ttggtggcca tcacgctctt gccgctcccg ggcaggccga ggatctgaat
     8521 gttggtcttg cgccaggtat cacgcggaac cagcaccggc ttgccctgct catccttacc
     8581 cagaaacacc gcctgctcct gccgggcctg ctccgaggaa aaatcggcca gcgcattaac
     8641 cggtaccggc tccccgaaat gcaggtggcg cacgtcggtc atctctcctg tccgctggct
     8701 gctgcgccgg ctctcctgat tcagtttcgc gttcaccgcc tcgtcagcag ccgcaaaatg
     8761 accgtcaagc agcaggaacg ccagcagccg cgccaacaca atcccgacag ccagcccgcc
     8821 cgcgcaccag gcaatcccga acaggttagc cggctgcagc aggtaataat ctgcgtttgc
     8881 gtagaggtcc ggacgcagga cggtttctcc ccagctctcg ataaacacgg cgacaaccag
     8941 cccccccaga agggccagac cggccgcgcc ggtcagccag tacgcgtagt tccagacatg
     9001 tcgacgtgcc ctgaccatcc gcccgggcga atccagcgag gccagcaccg aaaatggcgc
     9061 tggccgttta ttatcgcgct tatagcgatc ggcatactcc cgtcgctcct tatgactcag
     9121 ccccacaaaa cgcatccgtc cggcccaaag ttcagccaga ccgaaaaaca ggccgataac
     9181 taggaaagcc ccggccagac cgtaatactc ctggtacagc cagctgaccc acggataaat
     9241 ctgccgggcc agctccggaa tgtgccactt ccaggcaagc gaatccgtat taaacatgtt
     9301 ctctctcctt cagacgatgg tttgccggcg tgatgtgcag cccccgctgc acaagccgcg
     9361 gacatgcccc aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac
     9421 cacggaggcc agcctctttc cgccccatgc ggaaaaggcg cagccccgga ggaacaacga
     9481 gcacgcccca gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc
     9541 ggcccccgtc agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt
     9601 tgctgcttcg ccaggtgcgt ccagcaccgt ggatttttgt gggtggtcag gcgtgagcct
     9661 gacgggggcc gggtgaattt ctggcgtggc cggcgtccag ccggacggag ccagaaagag
     9721 ggggagagac cgtgcgtcgg tggcgtccag ccagcgtaag caggggatat ccccctgagg
     9781 tcagccacgc cctcgcgggg atgacctgta tttctccgta aggagaaaaa agcaacagag
     9841 ataggttaac gtaaaatgtt cagcgaaaaa ccgatatgat tcacctatcc tggtaattta
     9901 ttatcaggga atataaagac ttaatcaggg gacaatatgc cacatcagcg cgtccggtgt
     9961 ccgtcttgta ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct acgctccggc
    10021 cgtgagactg cggcgggcgc tgcggacaca tgcaaagtta cccacagatt ccgtgaataa
    10081 gcaggggact aacatgtgag gcaaaacggc aaggccacgc aggtggcgtt tttccatagg
    10141 ctccgccctc ctgactgagt tcacataaac agacgctttt tcggtgcatc tgtgggagcc
    10201 ggtggctcaa ccatgaatca gtcaggagtg gcgaaacccg acaggactta aagatcatca
    10261 ccgtttccga tgaatcgctc cctcttgcgc tctcctgttc caaccctgcc gtttcccgga
    10321 tacctgttcc gcctttctcc cttgcgggaa gtgtggcgct ttctcatagc tcacactctg
    10381 gtatctcggc tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aaccccccgt
    10441 tcagcccgac tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cggaaaagca
    10501 cgcaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc
    10561 taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga aggacagatt
    10621 tggttgctgt gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa
    10681 ccttcgatca aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc
    10741 agaaaaaaag gatctcaaga agatcctttg atcttttcta ctgaaccgcg atccccgtca
    10801 gttcagagga ggacgatggt gccaccgttc caccctgaac atcccggata tagtttgcct
    10861 gacatgcgac cagaagaaag cctgaaaaaa tcaatataaa aagaaaaaca acctttacct
    10921 ttttcatata ccttccctgt atttaaaaac cccggtattc atccctgaac accggaagtc
    10981 tgtatttcaa atcatccttg ataacttttg cttaccagtt accatcccgg taaacaaagg
    11041 atacaaagta atcaaattta ctaatatcga ttacattttt aaagtgtggc tgtaatatcg
    11101 aaacccatga tttttcaaca tcaaaccaac cattattaat attatccttt aaaggcatcc
    11161 ccagactttc aatgacagaa ccatcatcac cgaagtcttt tgagtattca ccacctttaa
    11221 actcttcggt tttcttatca aaccaatgaa tatgtaattt aagccccata tttacctctt
    11281 aaagatattt tttaatgttt cgttttggat ccggcccttt aacctgctta cccgtttttg
    11341 gatcgaatgc cccgaggtgt tcgccatcac tggcgcggta cccttcaagc tcaccgtgct
    11401 gggaatccca ttcataaatt ttacgctttt tatcaccata ccatcgggcc cgcttaccac
    11461 caccaccttg tttaggggtt ttaggtttac cctctttcaa ctctcccaac cccttaatat
    11521 cttcagtctt gggatcagga aaataatcat ggccgtaatc tttagttccc ttacgaggtt
    11581 ttttcttttc ctcatttaat ttattttctg catccttcgc ttttttctcc ttttgtttcc
    11641 ggctctccat cgccccggaa agcgcagcct tatccctgga taaggattca ttcatttcgt
    11701 ttaaagatgt ctgggcagct tttaatttct cattctgatt ctgaatatcc ttatcaagat
    11761 agcgaacctg atcctgccac ccggagccat attcatgagg cgggttataa gtcacaaaat
    11821 catctttatt tgcctctgcc tcttttacct ttttatcggc ttcggggtct gacgctcagt
    11881 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11941 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    12001 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    12061 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    12121 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    12181 cagggcactg ttgcaaagtt agcgatgagg cagccttttg tcttattcaa aggccttaca
    12241 tttcaaaaac tctgcttacc aggcgcattt cgcccagggg atcaccataa taaaatgctg
    12301 aggcctggcc tttgcgtagt gcacgcatca cctcaatacc tttgatggtg gcgtaagccg
    12361 tcttcatgga tttaaatccc agcgtggcgc cgattatccg tttcagtttg ccatgatcgc
    12421 attcaatcac gttgttccgg tacttaatct gtcggtgttc aacgtcagac gggcaccggc
    12481 cttcgcgttt gagcagagca agcgcgcgac cataggcggg cgctttatcc gtgttgatga
    12541 atcgcgggat ctgccacttc ttcacgttgt tgaggatttt acccagaaac cggtatgcag
    12601 ctttgctgtt acgacgggag gagagataaa aatcgacagt gcggccccgg ctgtcgacgg
    12661 cccggtacag atacgcccag cggccattga ccttcacgta ggtttcatcc atgtgccacg
    12721 ggcaaagatc ggaagggtta cgccagtacc agcgcagccg tttttccatt tcaggcgcat
    12781 aacgctgaac ccagcggtaa atcgtggagt gatcgacatt cactccgcgt tcagccagca
    12841 tctcctgcag ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccaccgaa
    12901 tgatgtcacg ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa
    12961 ggggatgata agtttatcac caccgactat ttgcaacagt gccgtttcca aaaggtcgtt
    13021 gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca gcaaatcaat
    13081 atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta cggccagcaa
    13141 cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag tcgatacttc
    13201 ggcgatcacc gcttccctca tgatgtttaa cgtttgacat gaggggcggc caagggcgcc
    13261 agcccttgga cgtccccctc gatggaaggg ttaggcaaca ctgcgtgttc gctcgaatgc
    13321 ctggcgtgtt tgaaccatgt acacggctgg accatctggg gtggttacgg taccttgcct
    13381 ctcaaacccc gctttctcgt agcatcggat cgctcgcaag ttgctcggcg acgggtccgt
    13441 ttggatcttg gtgacctcgg gatcattgaa cagcaactca accagagctc gaaccagctt
    13501 ggttcccaag cctttgccca gttgtgatgc attcgccagt aactggtcta ttccgcgtac
    13561 tcctggatcg gtttcttctt cccaccatcc gtccccgctt ccaagagcaa cgtacgactg
    13621 ggcataccca atcggct
//
