LOCUS       NZ_CP070532             4096 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain EC48293 plasmid p48293_Col, complete
            sequence.
ACCESSION   NZ_CP070532
VERSION     NZ_CP070532.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933270
            Assembly: GCF_016939555.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939555.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 03:48:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,165
            CDSs (total)                      :: 5,051
            Genes (coding)                    :: 4,953
            CDSs (with protein)               :: 4,953
            Genes (RNA)                       :: 114
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 98
            CDSs (without protein)            :: 98
            Pseudo Genes (ambiguous residues) :: 0 of 98
            Pseudo Genes (frameshifted)       :: 35 of 98
            Pseudo Genes (incomplete)         :: 70 of 98
            Pseudo Genes (internal stop)      :: 9 of 98
            Pseudo Genes (multiple problems)  :: 15 of 98
            Pseudo Genes (short protein)      :: 1 of 98
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070532.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="EC48293"
                     /isolation_source="Sputum-aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p48293_Col"
                     /geo_loc_name="Czech Republic: Praha"
                     /lat_lon="50.07 N 14.35 E"
                     /collection_date="2019-01-02"
                     /collected_by="Fakultn nemocnice Krlovsk Vinohrady"
     gene            41..199
                     /locus_tag="JW293_RS25975"
     CDS             41..199
                     /locus_tag="JW293_RS25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032670878.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHEKSLLAAAISNWRYTRDKL
                     RGLEVWA"
                     /name="hypothetical protein"
     gene            complement(436..705)
                     /locus_tag="JW293_RS01910"
                     /old_locus_tag="JW293_01915"
     CDS             complement(436..705)
                     /locus_tag="JW293_RS01910"
                     /old_locus_tag="JW293_01915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_079839079.1"
                     /translation="MNGQEFRLLLTEHGEKEAVLLFGKKSFVAVPDTQNLYGGCYYLYL
                     EDAHKNELLIELNESDLYPGGAIELQHNGGRKIHCEILKVRWSR"
                     /name="hypothetical protein"
     gene            787..1020
                     /locus_tag="JW293_RS01915"
                     /old_locus_tag="JW293_01920"
     CDS             787..1020
                     /locus_tag="JW293_RS01915"
                     /old_locus_tag="JW293_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461706.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_001036746.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1042..1269
                     /locus_tag="JW293_RS01920"
                     /old_locus_tag="JW293_01925"
     CDS             1042..1269
                     /locus_tag="JW293_RS01920"
                     /old_locus_tag="JW293_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_000719360.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            complement(1799..2008)
                     /locus_tag="JW293_RS01925"
                     /old_locus_tag="JW293_01930"
     CDS             complement(1799..2008)
                     /locus_tag="JW293_RS01925"
                     /old_locus_tag="JW293_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001632067.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2012..2497)
                     /locus_tag="JW293_RS01930"
                     /old_locus_tag="JW293_01935"
     CDS             complement(2012..2497)
                     /locus_tag="JW293_RS01930"
                     /old_locus_tag="JW293_01935"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_012782630.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<2532..3236)
                     /locus_tag="JW293_RS25980"
                     /old_locus_tag="JW293_01940"
                     /pseudo=""
     CDS             complement(<2532..3236)
                     /locus_tag="JW293_RS25980"
                     /old_locus_tag="JW293_01940"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3175..3498)
                     /locus_tag="JW293_RS01940"
                     /old_locus_tag="JW293_01945"
     CDS             complement(3175..3498)
                     /locus_tag="JW293_RS01940"
                     /old_locus_tag="JW293_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001555769.1"
                     /translation="MLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3607..3685)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          2..369
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.654"
                     /coverage="99.0"
                     /evalue="1.47e-78"
     Typing          3761..4096
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.058"
                     /coverage="94.0"
                     /evalue="3.9899999999999997e-84"
     Typing          complement(1690..3185)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.998"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 actcagttcc gcgaaggcag ttcgctccag gattgactgt atgcacgaac cccccgttca
       61 gtccgactgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacacga
      121 aaaatcgcta ctggcagcag ccattagtaa ctggaggtat acgagagaca aattaagagg
      181 tcttgaagta tgggcctgag agaggctaca ctgaaagaac agttttggtt tctgcggtct
      241 cgtataccca gttacctggt taagcagttc cccaactgac ttaaccttcg atcaaaccac
      301 ctccccaggt ggttttttcg ttttcagagc aagagattac gactgtaacg acaggatcac
      361 aagaagatca tttactcaga gagactaact accactatgt ttgcctatca acatacttag
      421 gaaacattac ttgagttaac gactccacct tacttttagt atctcgcaat gaatcttccg
      481 accgccgttg tgttgaagtt ctattgcgcc accggggtat aaatcacttt cgtttaactc
      541 aattaaaagc tcgttcttgt gagcatcctc taaataaagg taataacacc caccataaag
      601 gttctgcgta tctggcacgg caacaaaaga tttctttccg aacagtaaaa cagcctcttt
      661 ttctccatgc tctgtcagca ataatcgaaa ttcttgtcca ttcaatcctc ttttcgcatg
      721 aaaaaacgac ctatacttag atggttagtg accatatcca ctttaccttt aagcagggaa
      781 cctattatga ataaaaaaac gctgttggcg gccatgatgg tcgcgttggt gacctttgcc
      841 gctgctggat gttcttcaaa ccaagaaata aaaaccacag atgatcgcac tatcgttaca
      901 gacggcaaac ccaaagttga cagtgatacg ggattagtat cctacaaaga tgcccataca
      961 ggccgaaccg aacagatcaa ccgtgacaaa attaccaaca tgagtgaact agacaactga
     1021 tacggataaa aggagctggc tttgaaaaag atttttgttt cagtgttcgc agcggcagtt
     1081 gcgctttcag ccctaaccgg ctgcacgcgc acaagctatg ccatccacac caacgacggg
     1141 cgtacaattg tcagcgacgg caagcctaca gaatcagact caggcctgct gggctataaa
     1201 gacgcaaatg gtgtcaaaca gcagatcaat aaagccgacg ttaaagaagt ctctgaaatt
     1261 ccgcattgag gcatcgctct ctccgaaaag ggccgctaat gcggcccttt tctttatctg
     1321 ccccaacaac aggcaaaaaa agcccccgct gggggccaaa tacctgcagt ggctggatag
     1381 tactgcattc ctgatgaagg agacgaataa gtatgggccg gatttttgcg tctgcaggct
     1441 cggtcgccac aatttttccg tacccgccag gcatcatgct tttaccataa catcccttgc
     1501 ccgcacctct ccggacgggt tcagcgcgaa tgcggcgtca gtcctgcaaa accgacagta
     1561 aaccaccccg caaaccgtgc attctgcgcc tgataaatct cacccgactg catagccatg
     1621 cagccgggtg aatggcaccg ggtttcggcg cagatcctca atgaaagccc cgttttatcc
     1681 ggttatcaca gcgacggacc gtcatactcc tgctgacgct gcgatcgttc cagctccagc
     1741 gctttctggt gctgcgctgc gcgctcctgc tcccggtgtg cctcgatgtc gttcagcctt
     1801 acgacaaccg gcgcagctgc tcgcttaaac tcagcacctg cgtgctcaag tccgtgacgc
     1861 gctcgctcag cgctgcgttc tcccgttgca taagaccaca catcgtccgc cattccgcga
     1921 aggcgctctc ccactcgtcc agccttttcg agtagtcctg ctgtagctgc tctaatgcgc
     1981 tcagcaactg tttttccagt tccgtcatgt gctatttccc cgccagtatc atccagctcg
     2041 aatcctctcc gaaccgtccc gcgtccgggt tcaccctcac gcagcggcgt cgttcctctc
     2101 cgcaggtcgt gagctgcacg ccgctgttcc tctcggacag catggcctgc gtgctcttct
     2161 gctcccggat ggtcgtgtaa ttgtcgagta tctgctgccc ctgccaccat agaatgcccg
     2221 cacttgaggc tatcagcagc acggagacca ggacgatggt cagccacgtc tggctgacca
     2281 tgcgcagcat ccctttcgta cgctggctca tggctgagga cagcatccgg tcgtggtcgt
     2341 ggatggcggc gctgattctc ttctcgcttt cgttcagttc cgctctgaca gacttctcgt
     2401 gctcgctgaa tgcggctttc agcatctcgc cggtagtctg ctgctgcact ttcgatttct
     2461 gctctaagtc cttcgccagc gttaaaagac tgttcataga tggctccctt cagtcggatg
     2521 tttcgccccc cgtccgggtc ggcaatgctg atgctgcttt ttgtggtgcg aaccacctca
     2581 aaacctgcgc tttccagcgc ctcagtgacg tcctgacgcg ttttcagctc cccggacgag
     2641 gctaaggcca gtaaaccccg cgtaatcgcc tgagcggctt cctgcttcgt ttccggcagc
     2701 gcggacggcg tgaccagtgc ccgccggttt tccggcgcgt tcgggtcgtg cagccccaga
     2761 ctgccgttca cgatggtctg ccaggcatca atgcgaggac ggtcggcgcg gtcatagtat
     2821 ggctggagcc gtttgccggt cagcagctcc gtgttcggga tcaggaagtt cagctccagc
     2881 cgccccttgt cccggtgctc aacccacagc acgctgtact ggtctttatc gagtccgggc
     2941 atcagaaccc gctcgaagct cgccatcagc gtttcacgct gcccgggcgg taagtcctgt
     3001 tcggcaaagg acagcacccc ggaggtgtac tttttggcgt agggcgaggc gtcgataagc
     3061 tcccggactt cctccggctt cccctgaaga acgctggcac cgtcgcgctg gcggtctttc
     3121 cccagcagat aatcaaccgg gccggcaccg cctccgcgtc cccggggatg aaacttaacg
     3181 atcatcgtca gccccctttt ccagtacggc atgccgcagc cgctcgagtc cggcatcgat
     3241 ggccatcagc gcggcgacga cctgcacgcg gtcgagaccg ctactgccac cggcgttaac
     3301 ctggcgggca atctggttga ggttgttccc catgccggca agctgacgaa gcagcgccgg
     3361 cgagatcgag ggaagtttgc cggcccgggc gggcttctcg tccaggcacg tctgccgcat
     3421 ccaggccgcg agctgtttac cgtcgcagcg ttccagcaga cggcggtgct catcttctgt
     3481 tacccacatc gtgagcattt tgctgcgttt gtctgccaat ccggcctcct gataacgccg
     3541 ggacgggcgg gaaatctttt cccggtcggc gcggtgttgg gcaagccgca ggcgcgtcag
     3601 tggcatttag cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt
     3661 atactggctt actatggtgg cacgacggga atcgggatga aatagtcagt catgaaaact
     3721 gtgagatgag gctcaggtgc gtaagaggct gatgcctgtc ccggtggata ttccgcttcc
     3781 tcgctcactg actcgctacg ctcggtcgtt cggctgcggc gagcggtacg gactcacgga
     3841 taaagacgtg gcggggaaat acgtgaatgc aggaggggaa gaaaggatgc gccgctgccg
     3901 gttttccata ggctccgccc ccctgacaag catcacagaa tctgacgctc aaatcagtgg
     3961 tggcgaaacc cgatgcggct taaagatacc aggcgtttcc ccctgatggc tccctcgtgc
     4021 tcccccgcat tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc
     4081 tcattccacg cctgac
//
