LOCUS       NZ_CP070527             5585 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KP47693 plasmid p47693_1, complete
            sequence.
ACCESSION   NZ_CP070527
VERSION     NZ_CP070527.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933269
            Assembly: GCF_016939535.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939535.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 02:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,399
            CDSs (total)                      :: 5,278
            Genes (coding)                    :: 5,153
            CDSs (with protein)               :: 5,153
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 57 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 28 of 125
            Pseudo Genes (multiple problems)  :: 14 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070527.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP47693"
                     /isolation_source="swab from around the abdominal drainage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p47693_1"
                     /geo_loc_name="Czech Republic: Praha"
                     /lat_lon="50.07 N 14.35 E"
                     /collection_date="2018-11-26"
                     /collected_by="Veobecn fakultn nemocnice v Praze"
     gene            32..277
                     /locus_tag="JW292_RS27095"
     CDS             32..277
                     /locus_tag="JW292_RS27095"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     gene            complement(459..790)
                     /gene="mobC"
                     /locus_tag="JW292_RS00645"
                     /old_locus_tag="JW292_00645"
                     /pseudo=""
     CDS             complement(459..790)
                     /gene="mobC"
                     /locus_tag="JW292_RS00645"
                     /old_locus_tag="JW292_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /name="mobC"
     gene            complement(780..956)
                     /locus_tag="JW292_RS00650"
                     /old_locus_tag="JW292_00650"
     CDS             complement(780..956)
                     /locus_tag="JW292_RS00650"
                     /old_locus_tag="JW292_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_165764108.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSV
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1596..1754
                     /locus_tag="JW292_RS27210"
     CDS             1596..1754
                     /locus_tag="JW292_RS27210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2114..2440
                     /locus_tag="JW292_RS00655"
                     /old_locus_tag="JW292_00655"
     CDS             2114..2440
                     /locus_tag="JW292_RS00655"
                     /old_locus_tag="JW292_00655"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197760.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINEMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            2466..3011
                     /locus_tag="JW292_RS00660"
                     /old_locus_tag="JW292_00660"
     CDS             2466..3011
                     /locus_tag="JW292_RS00660"
                     /old_locus_tag="JW292_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433973.1"
                     /translation="MFFKRYLTEKEIYKVALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3077..3427
                     /locus_tag="JW292_RS00665"
                     /old_locus_tag="JW292_00665"
     CDS             3077..3427
                     /locus_tag="JW292_RS00665"
                     /old_locus_tag="JW292_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3497..3763)
                     /locus_tag="JW292_RS00670"
                     /old_locus_tag="JW292_00670"
     CDS             complement(3497..3763)
                     /locus_tag="JW292_RS00670"
                     /old_locus_tag="JW292_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3747..3971)
                     /locus_tag="JW292_RS00675"
                     /old_locus_tag="JW292_00675"
     CDS             complement(3747..3971)
                     /locus_tag="JW292_RS00675"
                     /old_locus_tag="JW292_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4134..4262
                     /locus_tag="JW292_RS27100"
     CDS             4134..4262
                     /locus_tag="JW292_RS27100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            complement(4638..4850)
                     /locus_tag="JW292_RS00680"
                     /old_locus_tag="JW292_00680"
     CDS             complement(4638..4850)
                     /locus_tag="JW292_RS00680"
                     /old_locus_tag="JW292_00680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4857..5366)
                     /locus_tag="JW292_RS00685"
                     /old_locus_tag="JW292_00685"
     CDS             complement(4857..5366)
                     /locus_tag="JW292_RS00685"
                     /old_locus_tag="JW292_00685"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     Typing          complement(1043..1123)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.476"
                     /coverage="98.0"
                     /evalue="3.1e-22"
     Typing          1308..1705
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.801"
                     /coverage="91.0"
                     /evalue="6.52e-141"
     Typing          complement(3..469)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="9.78e-97"
     Typing          complement(4523..5583)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.718"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcgcgcgccg gttctccggt gcgttcgggt cgtgcagccc cagtcgcccg ttcaccacgg
       61 tctgccacgc atcgatgcgc ggacggtctg cccggtcgta gtacggctgg agacgcctgc
      121 cggtcagcag ttccgtgttc ggtaccagaa aattcagttc cagccgcccc ttgtcccggt
      181 gttcaaccca cagcacgctg tactggtctt tatcgagtcc gggcatcaga acccgctcga
      241 agctcgccat cagcttttca cgctggccgg gcggtaaatc ctgttcagca aaagacagga
      301 cgccggaggt gtactttttg gcgtagggcg aggcgtcgat aagctcccgg acctcctccg
      361 gctttccctg caggacgatg gccccgtcgc gctggcggtc tttccccagc agataatcca
      421 ccggcccggc accgccgcct cgccctcgcg gatgaaactt aacgatcatc gtctgccccc
      481 ttttccagca cggcatgccg cagccgctcg agtccggcat cgatggccat cagcgcggcc
      541 accacctgaa cccggtcagc gcctgaccac tggccgccgt taattggttg aggttgttcc
      601 ccatcccggc aagctggcgc agcaaaaccg gatcgatgga cggcagccgc aacgaccgcg
      661 ccgggcgcgt atcgagacag gtttcccgca tccacaccgc cagttgcctt ccattgcagc
      721 gctccagcag ctgctggtac tccccatcgg tcacccggat acctaaaaaa cgggtacgct
      781 taaccgccat cctctcctcc gatatccgca aaatgtgacc ggactgaacg ctcaagctct
      841 gccatctgct cccggcggta atcttcctcc tgtgcctgac gcatcagatt tgccatcact
      901 gccggatgcc aggatctttt ttttacgacg agcggcttct tcttcggctt ctgcattatc
      961 cctccttcgc aaacgccgga cggcacggaa atgttttccg ggacgcgccg gcgttggaca
     1021 agcgccagcg cgtcagtggt gttagcgggt ttcggggcgc agccctgaac cagtcacagc
     1081 gtttacgctg tgtatactgg cttactatgt tggcatgaga gcgagattca tgaaagtgcc
     1141 tcatgtggca ggtgtgatat gtgactcagg agcgtccgca ataccatgca cggacagatg
     1201 aaatgacgcc tcctcgctca ctcggtcgct gcgctcctgc cgttcggctg cggcgggcgt
     1261 gaccggctca cggataacgg gataaagcaa aggcgctcaa atcagaaagt gccggagaaa
     1321 aggatacggc gtggccgttt ttccataggc tccgcctccc ctgacgagca tcacagaaat
     1381 ggacgctcaa gtcagaggag gacgaaaccc gacaggactt aaagatgccg gtgatttctc
     1441 tccggcggct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccga atacctgttc
     1501 cgcctttttc cctttcggga agcgtggcgc tttctcatag ctcacgctgc tggtatctcg
     1561 gctcggttta ggtcgttcgc tccaagctgg gctgtatgca cgaacccccc gttcagcccg
     1621 actgctgcgc cctttccggt aactatcatc ttgagtccaa cccggacgga cacgcaaaaa
     1681 cgccactggc agcagccatt ggtaactgga tatatacgag agagatattg agaggtcttg
     1741 aagtgggtgc ctaactgcgg ctacactgaa agaacatttt tggtatctgc ggtctcgtat
     1801 acccagttac ccggttaagc agttccccaa ctgacttaac cttcgatcaa accgcctccc
     1861 caggcggttt tttcgtttac agtgcaagag attacgcgca gaaaaaaagg atctcaagaa
     1921 gatcctttaa atgatcgtca gttctgttat ccccggagct ccatccatcc cacggaaaaa
     1981 cacccgccac tgatgccctc agcgcactgc acatcatgta caaaccactc cctccctcct
     2041 tttttattgc gtttcttaat gcgcaatggt agctttgtgc gcaacaaaaa acgcaatagc
     2101 gagggggggg gtaatgagag aaagaacaaa tatcatgcta gatataggat taaagcaatc
     2161 cttacaacgg ctttcgaata aaaccaaaat gagtctaagt gatcttatta accagagatt
     2221 atcaggttca ctttcagttg aagaacgcat tggcgaaaag cctgaatgga tgagaaacct
     2281 tgatccctat ctcgccaatc acttttcaaa tattgagtta ccgctccctt caccattaaa
     2341 agaataccaa gaaaaaatgg atgagataga ttcaattatt aatgaaatga tggttctaaa
     2401 ggaatatttc agagctaagt tttacgacga aataaaatag gcacgctata cggaggcgga
     2461 gaattatgtt ttttaaacgg tatttaacgg agaaggaaat atacaaggta gcactcccaa
     2521 ttgttaggct tcaagggaga gttaacactt cattacgttt tttatccaaa tactcagatg
     2581 taaacaaaaa tggattaatt gcattacatc ttaatttatt acattttatc tcatacaaca
     2641 tacacggtgt agagtctctg aaaatcactc aagcaggcat aaacaaagct gtacaacaaa
     2701 gccctcacgc agatgaaata aaaaacattg cgcaggcaat aggacgcaaa ggacctgagt
     2761 acatcgaagg attaattaga aacccatggg ggaagaaaaa tgataatcca ctaatattta
     2821 taatgagcat tgaagaaagt ataaaagaaa cccttaactc aggttatatg ttatatagag
     2881 tagctcccat aactgagggt gaagcagctg ctttcatgga tgttcttaaa caattattat
     2941 tatttgagga taattttcgc ctttttggta gggacttggt ttataaaata gaaaaattaa
     3001 aaatcagata acaaagtatg gtgaacgaaa aatgaaagag aggatagtga aatgtgtaat
     3061 accaagattc tcaactatgt attggttaat tgcgttccta gcatttatat tccccggtcg
     3121 atcttttgct gacggttaca ccgcttacta ccgatgtaat ggtactcagg aagcatctct
     3181 tactgttaaa gataacaaac taagtatggt tgtaggtctt tctaagttta ataggcaact
     3241 ggatacttat agattcaaag aaaagggaat cgaagtagta atgcagagag ctgcttctga
     3301 tgataactct gaggccataa tgtcttttat tatgcctgac gttaacaata tgaccttatt
     3361 agttaaaaat aacggctaca ttatcgttaa gacaacctgc tggttaacta aaaccgacaa
     3421 acagtagaac acgtagttag tcgagtattt accataatac ctgttgtcgg catcaaaaaa
     3481 gcccgcatag cgggcttcag tcgtaaactt cgcggcgatg tccaatcgtg gcggcaagga
     3541 taacaagctc gtcatctcgg atctcacaca gaacgcgata atctcccacc cgatagcgcc
     3601 agaactcgcc cagctcacct ttgagcggct tccctgactg gcgaggatcg gcagcaactg
     3661 caatgcgtaa actcataaaa tccacaatcc gtcgtgcgtt ctgtttgtcc atcttgcgta
     3721 acgatttgag cgcccgatcg gaatagttaa tcgtccagac caagtgactt cctcacgtct
     3781 tcagacgaat gcactgcttc atcaccacgg cgaacgcgtg cagcagtttc tgctgaaaga
     3841 taataatcct ccaggtcttc cagatgcgcc agtattgctt cccgcgcata aaacgtcttt
     3901 gttctgccgg tttgcttcgc cagcgagtcc agacgggact caatctcatc agataaccgg
     3961 atagcaagca tataaacctc ccgaacacca tttgctatac atgtatatca acagtaccac
     4021 aatcaaccta aaagaacaaa aaataatctt gttagcccct cttgataatc cgtaacgcgg
     4081 tcaatatatc tagcttgagg gctttcctct taactattga aaggataacg attatgggtc
     4141 ttaagccggg tccgaaacga attgccgatt caacagggga acctgataaa cgccagcgtg
     4201 acaataaaaa aacgccggga aatactgaca aattgaaacc cagcaaatct tcaaaaaaat
     4261 aagtcagtag ttaaaagccc tccggggctt ttttgtatct gccgttcacg ctgattaaac
     4321 ataacacccg ttgcccgcac cgccccggac gggttcgccg cgaacggggt ggcagtcagg
     4381 taaaaccggc aacaaaccac ccgataaacc gccccgttcg cgacagataa attttacctg
     4441 gctgcatagc catgcagcca ggtgaatgac atccggtttc agcgtaaatg cccgatgaga
     4501 accctgtttt cctgcatttc agcccagcga cggaccgtca tagcttcgct gccgggagcg
     4561 cgcttccgtc agttcctttt cgtgctgccg ttgctcgtgc gcccgttctg cggccacggc
     4621 catctcatga cgctgcacta ccggctcaaa cgctgcagtt gcccgctcaa gcgttccacc
     4681 tgctgactca agcgcgtcac gcgctcgctc agcgccgcgt tctcccgttg cataagccct
     4741 gacattttcc gccattcccc gaaggcgctc tcccactcgt ccagcctttt cgagtagtcc
     4801 tgctgtagct gctctaatgc gctcagcaac tgtttttcca gctcggtcat cggcacttac
     4861 tccttcacca gtctcagcgc gttctgcttc gaccgcccgt tcccgaccgt ccagctctgc
     4921 gtcctgtcta cggccgtccc cttcggcagc accagaaaac gtccgttgct gtcttccagg
     4981 tacgtcacgc cccaggtctt cgcgttcagc ttcgacagcg ccgcgttctg ccggtcgatt
     5041 tcggccagat ttgacgctat caggctcccc tgataccaca acaccccgct caggctggcg
     5101 aacagcagtc ctgacaccat cgtgatggtc agccatgtcc gcccgaccat acgcatcacg
     5161 ctcaggcggt tcgactgcat ggccgcgctc ataccctgct cgtgggcgcg gatggcggcg
     5221 ctgattctct tcccgctttc gctcagttcc gctctgacag acttctcgtg ctcgctgaat
     5281 gcggctttca gcatttcgcc ggtactctgc tgctgcgctt tcgatttctg ctctaagtcc
     5341 ttcgccagcg ttaaaagact gttcatagat ggctccctta agtcggatgt ttcgccctcc
     5401 gtccgggtcg gcaatgctga tgctgctctt tgtggtgcgc acgacctcaa aacctgcgct
     5461 ttccagcgcc tcagtgacgt cctgacgcgt tttaagctcc cctgacgagg cgagggccag
     5521 taagccccgc gtaatagcct ctgcggcttc ctgcttcgtt ttgggtaacg aagatggcgt
     5581 cacca
//
