LOCUS       NZ_CP070526            10122 bp    DNA     circular CON 03-JUN-2024
DEFINITION  Klebsiella pneumoniae strain KP47693 plasmid p47693_ColRNAI,
            complete sequence.
ACCESSION   NZ_CP070526
VERSION     NZ_CP070526.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16933269
            Assembly: GCF_016939535.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Kraftova,L., Finianos,M., Studentova,V., Chudejova,K., Jakubu,V.,
            Zemlickova,H., Papagiannitsis,C.C., Bitar,I. and Hrabak,J.
  TITLE     Evidence of an epidemic spread of KPC-producing Enterobacterales in
            Czech hospitals
  JOURNAL   Sci Rep 11 (1), 15732 (2021)
   PUBMED   34344951
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Bitar,I.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-FEB-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial Assembly v. 1.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 250X
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_016939535.1-RS_2024_06_03
            Annotation Date                   :: 06/03/2024 02:52:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,399
            CDSs (total)                      :: 5,278
            Genes (coding)                    :: 5,153
            CDSs (with protein)               :: 5,153
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 57 of 125
            Pseudo Genes (incomplete)         :: 58 of 125
            Pseudo Genes (internal stop)      :: 28 of 125
            Pseudo Genes (multiple problems)  :: 14 of 125
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070526.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP47693"
                     /isolation_source="swab from around the abdominal drainage"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p47693_ColRNAI"
                     /geo_loc_name="Czech Republic: Praha"
                     /lat_lon="50.07 N 14.35 E"
                     /collection_date="2018-11-26"
                     /collected_by="Veobecn fakultn nemocnice v Praze"
     gene            join(8931..10122,1..494)
                     /locus_tag="JW292_RS00575"
                     /old_locus_tag="JW292_00575"
     CDS             join(8931..10122,1..494)
                     /locus_tag="JW292_RS00575"
                     /old_locus_tag="JW292_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            504..761
                     /locus_tag="JW292_RS00580"
                     /old_locus_tag="JW292_00580"
     CDS             504..761
                     /locus_tag="JW292_RS00580"
                     /old_locus_tag="JW292_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            846..1082
                     /locus_tag="JW292_RS00585"
                     /old_locus_tag="JW292_00585"
     CDS             846..1082
                     /locus_tag="JW292_RS00585"
                     /old_locus_tag="JW292_00585"
                     /inference="COORDINATES: protein motif:HMM:NF014457.4"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_123836186.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANGTVAKLAMRQPFVLFKGLTF
                     QKLCLPGAFRPGDHHNKMLRPGLCVVHASPQYL"
                     /name="colicin release lysis protein"
     gene            complement(973..1677)
                     /locus_tag="JW292_RS00590"
                     /old_locus_tag="JW292_00590"
     CDS             complement(973..1677)
                     /locus_tag="JW292_RS00590"
                     /old_locus_tag="JW292_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            1713..1823
                     /locus_tag="JW292_RS00595"
                     /old_locus_tag="JW292_00595"
     CDS             1713..1823
                     /locus_tag="JW292_RS00595"
                     /old_locus_tag="JW292_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_116938357.1"
                     /translation="MTTDYLQQCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            3304..5268
                     /locus_tag="JW292_RS00600"
                     /old_locus_tag="JW292_00600"
     CDS             3304..5268
                     /locus_tag="JW292_RS00600"
                     /old_locus_tag="JW292_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            5268..5999
                     /locus_tag="JW292_RS00605"
                     /old_locus_tag="JW292_00605"
     CDS             5268..5999
                     /locus_tag="JW292_RS00605"
                     /old_locus_tag="JW292_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            6006..6536
                     /locus_tag="JW292_RS00610"
                     /old_locus_tag="JW292_00610"
     CDS             6006..6536
                     /locus_tag="JW292_RS00610"
                     /old_locus_tag="JW292_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6564..6743)
                     /locus_tag="JW292_RS00615"
                     /old_locus_tag="JW292_00615"
     CDS             complement(6564..6743)
                     /locus_tag="JW292_RS00615"
                     /old_locus_tag="JW292_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6812..7189
                     /locus_tag="JW292_RS00620"
                     /old_locus_tag="JW292_00620"
     CDS             6812..7189
                     /locus_tag="JW292_RS00620"
                     /old_locus_tag="JW292_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            complement(7251..7550)
                     /locus_tag="JW292_RS00625"
                     /old_locus_tag="JW292_00625"
     CDS             complement(7251..7550)
                     /locus_tag="JW292_RS00625"
                     /old_locus_tag="JW292_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7540..7803)
                     /locus_tag="JW292_RS00630"
                     /old_locus_tag="JW292_00630"
     CDS             complement(7540..7803)
                     /locus_tag="JW292_RS00630"
                     /old_locus_tag="JW292_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            complement(8109..8528)
                     /locus_tag="JW292_RS00635"
                     /old_locus_tag="JW292_00635"
     CDS             complement(8109..8528)
                     /locus_tag="JW292_RS00635"
                     /old_locus_tag="JW292_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     BGC             3931..10122
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1927..2505)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5269..5996
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.32e-150"
ORIGIN
        1 atcaggttcg ctatcttgat aaggatattc agaatcagaa tgagaaatta aaagctgccc
       61 agacatcttt aaacgaaatg aatgaatcct tatccaggga taaggctgcg ctttccgggg
      121 cgatggagag ccggaaacaa aaggagaaaa aagcgaagga tgcagaaaat aaattaaatg
      181 aggaaaagaa aaaacctcgt aagggaacta aagattacgg ccatgattat tttcctgatc
      241 ccaagactga agatattaag gggttgggag agttgaaaga gggtaaacct aaaaccccta
      301 aacaaggtgg tggtggtaag cgggcccgat ggtatggtga taaaaagcgt aaaatttatg
      361 aatgggattc ccagcacggt gagcttgaag ggtaccgcgc cagtgatggc gaacacctcg
      421 gggcattcga tccaaaaacg ggtaagcagg ttaaagggcc ggatccaaaa cgaaacatta
      481 aaaaatatct ttaagaggta aatatggggc ttaaattaca tattcattgg tttgataaga
      541 aaaccgaaga gtttaaaggt ggtgaatact caaaagactt cggtgatgat ggttctgtca
      601 ttgaaagtct ggggatgcct ttaaaggata atattaataa tggttggttt gatgttgaaa
      661 aatcatgggt ttcgatatta cagccacact ttaaaaatgt aatcgatatt agtaaatttg
      721 attactttgt atcctttgtt taccgggatg gtaactggta agcaaaagtt atcaaggatg
      781 atttgaaata cagacttccg gtgttcaggg atgaataccg gggtttttaa atacagggaa
      841 ggtatatgaa aaaggtaaag gttgtttttc tttttatatt gattttttca ggctttcttc
      901 tggtcgcatg tcaggcaaac ggcactgttg caaagttagc gatgaggcag ccttttgtct
      961 tattcaaagg ccttacattt caaaaactct gcttaccagg cgcatttcgc ccaggggatc
     1021 accataataa aatgctgagg cctggccttt gcgtagtgca cgcatcacct caataccttt
     1081 gatggtggcg taagccgtct tcatggattt aaatcccagc gtggcgccga ttatccgttt
     1141 cagtttgcca tgatcgcatt caatcacgtt gttccggtac ttaatctgtc ggtgttcaac
     1201 gtcagacggg caccggcctt cgcgtttgag cagagcaagc gcgcgaccat aggcgggcgc
     1261 tttatccgtg ttgatgaatc gcgggatctg ccacttcttc acgttgttga ggattttacc
     1321 cagaaaccgg tatgcagctt tgctgttacg acgggaggag agataaaaat cgacagtgcg
     1381 gccccggctg tcgacggccc ggtacagata cgcccagcgg ccattgacct tcacgtaggt
     1441 ttcatccatg tgccacgggc aaagatcgga agggttacgc cagtaccagc gcagccgttt
     1501 ttccatttca ggcgcataac gctgaaccca gcggtaaatc gtggagtgat cgacattcac
     1561 tccgcgttca gccagcatct cctgcagctc acggtaactg atgccgtatt tgcagtacca
     1621 gcgtacggcc cacagaatga tgtcacgctg aaaatgccgg cctttgaatg ggttcatgtg
     1681 cagctccatc agcaaaaggg gatgataagt ttatcaccac cgactatttg caacagtgcc
     1741 aggcaaacta tatccgggat gttcagggtg gaacggtggc accatcgtcc tcctctgaac
     1801 tgacggggat cgcggttcag tagaaaagat caaaggatct tcttgagatc ctttttttct
     1861 gcgcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg
     1921 ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc acagcaacca
     1981 aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac cgcgtgttta
     2041 gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg gcgttttgcg
     2101 tgcttttccg ggttggactc aagtgaacag ttaccggata aggcgcagca gtcgggctga
     2161 acggggggtt cttgcttaca gcccagcttg gagcgaacga cctacaccga gccgagatac
     2221 cagagtgtga gctatgagaa agcgccacac ttcccgcaag ggagaaaggc ggaacaggta
     2281 tccgggaaac ggcagggttg gaacaggaga gcgcaagagg gagcgattca tcggaaacgg
     2341 tgatgatctt taagtcctgt cgggtttcgc cactcctgac tgattcatgg ttgagccacc
     2401 ggctcccaca gatgcaccga aaaagcgtct gtttatgtga actcagtcag gagggcggag
     2461 cctatggaaa aacgccacct gcgtggcctt gccgttttgc ctcacatgtt agtcccctgc
     2521 ttattcacgg aatctgtggg taactttgca tgtgtccgca gcgcccgccg cagtctcacg
     2581 gccggagcgt agcgaccgag tgagcgagga ggcgcaattt ccccggcacc tacaagacgg
     2641 acaccggacg cgctgatgtg gcatattgtc ccctgattaa gtctttatat tccctgataa
     2701 taaattacca ggataggtga atcatatcgg tttttcgctg aacattttac gttaacctat
     2761 ctctgttgct tttttctcct tacggagaaa tacaggtcat ccccgcgagg gcgtggctga
     2821 cctcaggggg atatcccctg cttacgctgg ctggacgcca ccgacgcacg gtctctcccc
     2881 ctctttctgg ctccgtccgg ctggacgccg gccacgccag aaattcaccc ggcccccgtc
     2941 aggctcacgc ctgaccaccc acaaaaatcc acggtgctgg acgcacctgg cgaagcagca
     3001 aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct gacgggggcc
     3061 ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc tggggcgtgc
     3121 tcgttgttcc tccggggctg cgccttttcc gcatggggcg gaaagaggct ggcctccgtg
     3181 gtgaaagctg aacgcaatca ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc
     3241 cgcggcttgt gcagcggggg ctgcacatca cgccggcaaa ccatcgtctg aaggagagag
     3301 aacatgttta atacggattc gcttgcctgg aagtggcaca ttccggagct ggcccggcag
     3361 atttatccgt gggtcagctg gctgtaccag gagtattacg gtctggccgg ggctttccta
     3421 gttatcggcc tgtttttcgg tctggctgaa ctttgggccg gacggatgcg ttttgtgggg
     3481 ctgagtcata aggagcgacg ggagtatgcc gatcgctata agcgcgataa taaacggcca
     3541 gcgccatttt cggtgctggc ctcgctggat tcgcccgggc ggatggtcag ggcacgtcga
     3601 catgtctgga actacgcgta ctggctgacc ggcgcggccg gtctggccct tctggggggg
     3661 ctggttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgtcc ggacctctac
     3721 gcaaacgcag attattacct gctgcagccg gctaacctgt tcgggattgc ctggtgcgcg
     3781 ggcgggctgg ctgtcgggat tgtgttggcg cggctgctgg cgttcctgct gcttgacggt
     3841 cattttgcgg ctgctgacga ggcggtgaac gcgaaactga atcaggagag ccggcgcagc
     3901 agccagcgga caggagagat gaccgacgtg cgccacctgc atttcgggga gccggtaccg
     3961 gttaatgcgc tggccgattt ttcctcggag caggcccggc aggagcaggc ggtgtttctg
     4021 ggtaaggatg agcagggcaa gccggtgctg gttccgcgtg atacctggcg caagaccaac
     4081 attcagatcc tcggcctgcc cgggagcggc aagagcgtga tggccaccaa cgcgctcatt
     4141 cgctgcgtac gcgatttcgg ggacgcggtg gtgtacttcg accccaaagg ggacgcatgg
     4201 gcgccgcacg tcttccgggc gcactgtccg gacttcaccc tgctggatct gcggccgggg
     4261 aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgcgctgaa aaacctgctg
     4321 attgccgggc ttaacctggg cgagaccggc gacgtggcgg accactaccg catcagcgag
     4381 cagaaagcgg cgaagctgat tgcggagcag tttccgcagg gggcaaacat tcagcaggtg
     4441 ctggcggcgg cgtacgcgct gccggaggcc ctgaaaaagg acgtgaaggg gttgatcact
     4501 aggctggaaa acgtcgctga cctgagcgtc ctgcagacgg acagtggcat cgatgtggcg
     4561 gggattatca acggcggcgg ctgcctgtac gttatcggct cgatggacga cgaggcggtg
     4621 atccgtgtgc aaaaaatgct gtttgcccgc tgcgcgcaaa tcatcattgc gcgggacgag
     4681 ttccgggagt ggccacacgt cagcgtgatg ctcgatgaaa ttaaatatct gctgtcgaag
     4741 tatgtactga acgcactggg gacgctgcgc agccgggact gcaatctgtt gctcgcgcac
     4801 cagtcgctgg gcgatttcgg gcagtgcggg caggatctgc ccgctgaatt tgtgaaaacc
     4861 acggtactgg ataacacgcc gatccgctgg ttttaccggg cggccagcct ggagtccgcg
     4921 cagtgggcgg cggggcagac cggggaaatc agggtggacg tggagcgccg ccgtgccagc
     4981 cgggaggcgg ggaacgtgga gcatatcagc ggggacagct tcatccagaa ggaggcgcgg
     5041 ccgctgtttg acgtgaacac cctgcaacac ctgccggacg ggttcgcggt catgaccggg
     5101 ctgggtgtgg cccggatggc gttcagcagt ccgctgcggg ttgagcgccg ggaaatcccg
     5161 ttgcagcgtt tcccggtgct ggcgaaaacc gatcccctgg cggagttccg gcccccggcc
     5221 gacggcggcg ggccgaaagc ggatgatgat tttgcgggga tttactgatg gcgctggagc
     5281 tgtacaacgt cagccacgcg aaacgacagg cgcggaacgc ggagaaaacc cggctgaccc
     5341 tgcgctggtt gcgggaagag ctgtgttcca cggcggagct ggttgcgcgg cggctgggga
     5401 ttgcggcggt gcagccggtc taccgcttcc tcgacagcct ggtggcgaaa gggctgctgg
     5461 tccggaccaa atatccggtg gacggccgtc aggtcagcgt gtgggggctg acgccgcatg
     5521 gcgtggcgtt ctcgttcgac gaggacgaac cgctggcgga tgttattccg tttcagccgt
     5581 caagggtgtc ggcagcacat ctgccgcacc ggctggccgt gcaatccctg cgccttgata
     5641 tggaagccag aggggccacc ggctggcgca ttctgcaccg tctgagtctt aaaggcatga
     5701 aagtgccgga cgccctggcg gagctggacg gtaaaacggt ggcgtttgag gtggagcgca
     5761 ccgtcaagag ccgtcgccgg tatcaggaag tggtgtccgg gtatctgttc aaccgcaaag
     5821 ctaacggcat cgatgaaatc tggtacatct gcccggaccg ggcgacgcag gtcagggtgc
     5881 agcgggcaat cctgtcggtg gatgaaatcg tgaacccaca gaccggcgag gcccgcaaaa
     5941 cggcagagct ggatcgtgaa cggttgtttg cgtgctttaa atttatgaca acggagtgag
     6001 ggattatggc gctgtacatg gtatttattg aagacgcgga ggaccggaac ggcgagcgct
     6061 gcatcatgcc gaccagcgag catctggaag atatggtggt gctcgcctgg cagaccgggg
     6121 cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa tccatcggga
     6181 tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcact ggtaaaaatc
     6241 cgcaatggga acggaacaaa cccacaggaa tgaagctgtg tgattacctc tatcgcaagg
     6301 tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag tgctcaccgg
     6361 agcagattaa cgcgatgatt gacgtggtga gtgagcggca cggggaaagc gcggtgaacg
     6421 agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac gtgccggtac
     6481 ctgaactgcc ggaggacaaa cctgcccgcc ggtggtggcc gttcggcaaa aaataaatgc
     6541 cagcaccggc catgccggtg ctgttattcc tccccgagct tatcccgcat cacctgccag
     6601 agtgtttccg catcctcgtg tagccgctcg cacatgtcgg ccggggtatc catatccagc
     6661 tcattcagtt tatcgagcaa tagcagcgac tgctccttaa taaatttagc catattgagg
     6721 gctgtttttt cctgttttgt catgattctt tccttccggg catggtgtgc gcctgtcagg
     6781 tatactgtct caaaaatagc ggggtgagac aatggcgtgg gtgacggtga gacaggcgac
     6841 ggaactgacg ggtaaagccc gaagttccct gtatcgggat atggcgaaag gccgcgtatc
     6901 ataccgtacg gaagctgatg gcggccgggt ggtggatacc agcgagctta tccgggtgta
     6961 cggtgagtta agacaggatg agactcacga gcgggacgag ttgagactgt cgggtgagac
     7021 tgaaaaaatg gcctccgacg cgttgatcgc ggagataaaa gcactcaggg aggaggtggc
     7081 cgggctacgg caggaaatgc agtccatgag actgctggaa cacaaaccgg agacagcaga
     7141 gaccccggcc ggaacaccgc gccggtggtg gccgtgggga aaacgctgac gcgctgtgct
     7201 tgtccaaccg gcgacggcca cgcaatgaat tgcgtccccg tcaccggtta ttagagaaca
     7261 atatggggat tctggcgctg gccgaccacg gccagaaccc ggacttttct ggtctggtca
     7321 tcaacatcat agaggacatg ctgtcccatc agactgatcc ggcgaacgtc ctcgccatag
     7381 cgtgagtcca cttcatagag tcgcgggaag tcggccagtt tgtccgccgc agcgagaacc
     7441 cggtcggtaa cgttctgcgc atcccacagg gatgaatttt ccgcgatata gtcaaacagc
     7501 gtcgttacat cctcgatggc tccctgcttc cattccacgt catacggcat cgcgcagttc
     7561 ctgataacgg ccatgcaggc gctggcggag ttgttccgtg gtcatacccg gacgcgtgtc
     7621 agcacgggcg gcggtcactt tggcgcgaac ctgatcttca tactctgcct gggccagttc
     7681 ttcgttagcc agtcgctgca acagcgcagc tttgtccggg ccaccttccg gcagtatgcg
     7741 gggcaggctt tcagccagtt cccgaagaaa gttggcgttt tcgtagtgtg tagtgttgct
     7801 cattgcggag cctcccgggt taatgcggca atatgaagga agtttagcag ggggcggtct
     7861 gaaagaatat ggtcgtgtca gtcaggctga tgaagcagaa agtcggtaaa aaagggctta
     7921 cggcatccat tttacgtcaa aacatatgcc atgcagttgc gtgcataggc atgcattagg
     7981 ataaaattta tcgggcgcgt ttccggcagt tttccggggg ggttgttgcc tgttttatcc
     8041 cgtagccgcc ggaaacgccc tgagcccgtc tgagcggtgc gcgtaatgac gcgttatggt
     8101 aaatccactt actgtctgcc ttcatagcca tcgagataaa cagctgaaat ggtgtcagcc
     8161 agtagccttg attttttcgg tgtgaggtct ttgctgcggt aatcggtacc cgcaaattgt
     8221 gttgtgcctt ggagtgagtt taaaaattcc tgactgtgaa actgctgaat gcgttgctgc
     8281 acatcactcc gtgattttcc ggtcgcttta tcctttgcgc ccatatccca gaactgccgg
     8341 aaaaaaggca ccatcattcg tgttgtgtca gctttctggg tggaaaaatt gggatcaaaa
     8401 ccgtcatctg aagcatatac gtaatgccgg gtatgctgtt ccacgctgcc ctttggagca
     8461 caggcagtga gagttgctgt cgctgttatc atggcgataa gacacagttt gctgccggaa
     8521 cgtctcatga gcgtctcctt acctttaatt cttttttctc attgagttat atatccgcag
     8581 gctatcttat catcagtttc tgaccgtgtt cagtgaccga acaacccgaa ccagaccccc
     8641 cgtttctttt cctgtcccgg agggggcttt ctggtatctc atataatgat ttcagtaaaa
     8701 catactgagg ccggagaacg gcgccgtttg tgctggtttt tgttgcatct ggaggggatt
     8761 gcgcatggtg tgagtggctg tttttttgat cattttcaca aaaacgcaaa tttacagact
     8821 tgacatgaaa aacaaggcag cgtaattttt gtactgtata taaaaccagt ggttatatgt
     8881 acagtatttt attttagttt attgttttaa aagtcaaaga ggaaaagtat atgagtggcg
     8941 gagacggacg aggtccgggt aattcaggtc tgggacacaa tggtggtcag gccagtggga
     9001 atgtgaacgg tacatcaggt aaaggtggcc cttcatcagg tggtggtacg gatccaaaca
     9061 gcgggccggg ctggggtacg acgcatacac ctaacgggga tattcataac tataacccgg
     9121 gggagtttgg taacggcggg agtaaacccg gtggtaatgg cggtaacagc ggcaatcata
     9181 gcgggagctc tggtggcgga cagtcttcgg ccaccgcgat ggccttcggt ctgcctgccc
     9241 tggccactcc gggggctgaa ggactggctt tatccgtttc cggcgatgcg ttgtcggccg
     9301 ctgttgctga tgtgctggct gccctgaaag gtccgtttaa gtttggtctg tgggggattg
     9361 cgatttacgg agtgctgcct tctgagatag caaaagatga tccgaaaatg atgtcaaaaa
     9421 tcgtgacgtc attgccggca gacacggtaa cggagacgcc ggtcagctcc ctgccgctgg
     9481 accaggcgac ggtcagcgtc actaaacgtg tggcggatat tgtgaaggac gagcggcagc
     9541 atattgcggt tgtcaccggc cggccaatga gtgttcctgt ggtggatgcg aaaccgacaa
     9601 aacgtccggg ggtattcagt gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac
     9661 ctaaaggtgt tccggccgcg aaagccccgc caaaaggcat tattgctgaa aaaggtgatt
     9721 cacgtccggc tggttttacg gccggtggta actcccgtga ggccgttatt cgtttcccga
     9781 aagagagcgg acagaaaccg gtctatgtgt cggtgacgga tgttcttact ccggcacagg
     9841 taaaacagcg tctggaggaa gaaaagcgtc gccagcaggc atgggacgct gctcacccgg
     9901 aagaggggct gaaaagagag tatgataaag cgaaagctga gctggatgct gaagataaaa
     9961 atattgcgac cttaaacagt cgcattgcat cgacagagaa ggcgatcccc ggtgcaaggg
    10021 ctgctgttca ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg
    10081 tgacttataa cccgcctcat gaatatggct ccgggtggca gg
//
