LOCUS       NZ_CP070463             3998 bp    DNA     circular CON 04-DEC-2023
DEFINITION  Klebsiella pneumoniae strain B28484 plasmid p4B28484, complete
            sequence.
ACCESSION   NZ_CP070463
VERSION     NZ_CP070463.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17915109
            Assembly: GCF_016925415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Multidrug resistant Klebsiella pneumoniae_B28484_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 94.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/04/2023 04:42:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,410
            CDSs (total)                      :: 5,281
            Genes (coding)                    :: 5,136
            CDSs (with protein)               :: 5,136
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 62 of 145
            Pseudo Genes (incomplete)         :: 82 of 145
            Pseudo Genes (internal stop)      :: 23 of 145
            Pseudo Genes (multiple problems)  :: 19 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070463.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B28484"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4B28484"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian Medical College"
     gene            complement(join(3979..3998,1..244))
                     /locus_tag="JT747_RS26910"
                     /old_locus_tag="JT747_26910"
     CDS             complement(join(3979..3998,1..244))
                     /locus_tag="JT747_RS26910"
                     /old_locus_tag="JT747_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(241..1095)
                     /locus_tag="JT747_RS26915"
                     /old_locus_tag="JT747_26915"
     CDS             complement(241..1095)
                     /locus_tag="JT747_RS26915"
                     /old_locus_tag="JT747_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     gene            complement(1632..2825)
                     /gene="mobQ"
                     /locus_tag="JT747_RS26920"
                     /old_locus_tag="JT747_26920"
     CDS             complement(1632..2825)
                     /gene="mobQ"
                     /locus_tag="JT747_RS26920"
                     /old_locus_tag="JT747_26920"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_096645250.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQIEWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            3069..3452
                     /locus_tag="JT747_RS26925"
                     /old_locus_tag="JT747_26925"
     CDS             3069..3452
                     /locus_tag="JT747_RS26925"
                     /old_locus_tag="JT747_26925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579763.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     gene            3456..3740
                     /locus_tag="JT747_RS26930"
                     /old_locus_tag="JT747_26930"
     CDS             3456..3740
                     /locus_tag="JT747_RS26930"
                     /old_locus_tag="JT747_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_071579762.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     Typing          complement(1816..2825)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.703"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 tatctaaggc aacataacgg cgatacaggg caacggctcc gtgatacgaa cagcccattt
       61 tgtcggctac ttgttgccat ttcagcccct ggcttcgtag ttcgtaagcc tgacgacgac
      121 gatccattgc tgttttttcg taatcgtcac gagattgaga aaaatatttt cttacagttc
      181 tttcactacg acctgtcatt tccattattt cttttgcttt tattttttta ccattaatga
      241 tcataaaacc cccttgctca ttaatatgtc taacatttca tttttaacat tggtttttcg
      301 aggttttttg gctctgtttg attggatttg tgaaaatgta tggctatcaa aatttttcca
      361 agtccaacgg gctacacttc taactgttgc ttttatctct ttgtattgta acgggttttg
      421 tggattgcaa aaagtgttta agccctgtgc ctttaatagt agttcgtttt cgaatttctc
      481 gaaagtggat atttttttga aatttaaaac cgttctgtaa gcatattgcc gtagatcttc
      541 aaacaaatta acattgcgac ctaaaccata agcctcgctt tttttctttt gtatttgagg
      601 cttttctaag tccacaaaat cagctaaata atccaggtca taaaggtcag cacctgacca
      661 gaacggcgac cagtgatcat tgagtgggtt ttttgtaatc aatccagcaa accccagatc
      721 tgcttttagt tttttcgcca tagccatttc aacggctgcg gcatacttca acggctttaa
      781 ccttgcttgt tgtgtcttac aaaccccggc tttcagcata tagccgtagt gacaacgacc
      841 tgtttttggt gtttttgtaa taaagtgagg tatgggtaag cccacctgct caaatgtata
      901 gaatgaattt ttcttatcta gatcaaagag taagcaagtt tgaatggctg gctgatttgc
      961 ctgtaaatac agcatttcaa gggcttttgc cttgttcctt atgcttaatc ccatatcgag
     1021 gttatttgta caataaggct tgttaggcat ttgattttta aaaaaatcga tgttatgttc
     1081 ttcttgtatt atcatttttt cagtcctttt tatctggccc tactctctgt tttctgtcgc
     1141 caaaacaaaa tcgcagaggg tggggttttt atttttatag atttttcaat cctgtaccag
     1201 ttctaaccga tctcaaaaat ttgtcaagtg tgcgtctaac catatcagat aacagcccaa
     1261 cgaagttggg gcagttcttt acatacggca gcaaagcatt actatcagat ctatggtgcg
     1321 aagcattacc gaagatagaa aaacaataaa aaaagaaaaa agagtattgt tcgaagaaaa
     1381 attggcgatt cgattttttt tggtgaatat atgaaaaaaa agaaagtgca gaaaaaacgg
     1441 aaaagaaata agcatagtaa attttttaaa atgcttatga agtcagttaa tgaagctgtg
     1501 gcaatacata aaggggaaat agaaccggcg aggatcacaa ctctaaaaat gtagtatctt
     1561 atagtatagt attaaataat attaagttgt attaagtgat actactatat aggaatatat
     1621 accactatat actacattcc aaatttcttc ttcctcttag gttgctgatc ttcgaattcc
     1681 ggcattggtt ccggctcagg ctcttcaatt tttggttgtg gttggttcat aggagacatt
     1741 ccagagcctg aacccatttt tccgcctcca ttatttccac cggttccggt tcctgtgcct
     1801 gttttaggtt ttgtttctaa ctctgtcttt aatatatgtt cgccaaattc atttattaca
     1861 taactactaa cgaaattttg aggatcttct ttttcttctt ttttattttt atttacatta
     1921 ggttttgatt taaatatatg ctgggtcttt ttgtataatg cctttaaatt atcaatgatt
     1981 gaccttgcaa gaggcttttt ctctattttc atagtttcct ctggctgagg tggtttggtt
     2041 tgctgtaagt atgttgcatt agctattttt aataataatg ctttttcttt ttgctccata
     2101 gctcttaatt tttgaccttt tcgccattgg tgcctgtgta tcctggtcat tggcgttctg
     2161 tccagttcaa tagtttttga aatccacttg gctttctctt tcggagtttt agcaaaaaca
     2221 gcctttcttt ctgcctcttc tttttggtct tttaatgaac gggagtcaat acgagattta
     2281 tgaccatatt tttttaatcg gtcatttgct attatttccc agttaactcg ccaggcttca
     2341 atgttttctc tattattcca ttctatttgt ttgttcccaa atccatttgg gctaacagtt
     2401 cttaaagtta gcattacatg agcatgaggg ttatgactat caagatcatg aaatgctatg
     2461 tctgcaatca ttccataatc cacgaaattt tcttggcagt aatcgaggac gatctgcttc
     2521 ttctggccgt cgtccagctc cttggggatg gctatgtcca gataacggca taattcggca
     2581 tcctttcgct tctcgccagc ctctacggca ttccagaggg tgcctgagtc cttcaccagt
     2641 tcggcaggtg tgccggcagg ggagaggata gcagagccga aaaatccctc tttttttgaa
     2701 taatcccagg ttgccccggt tcgattatct tttgtctttt ctcggttttg atatgcagat
     2761 aaataaaccg aagatttacc ttctgatctt cgtaattttt taaagctgat atgaaatgtc
     2821 gccatagtct accccttact ttttttgtct tttgaatttt ggtttttagc cccgcagggc
     2881 ccatacgtcg caagcgacgt gtaagtgggc attactaata ttattttttc ggttgcacct
     2941 caaaataata agtaatgaac cccaggcttc gcccaatatt aaatattatt tgttttattc
     3001 taaaatatat ttgactaaag taaataatgg aggtaataaa taacaaaata aataattagg
     3061 aggttattat gaataaactt ataacagagg cagaaggtgc aaaaatcgaa agtgttttta
     3121 aatacatatt agaggcatct gaaagcatat taatagagca tgggcatgtt attacggggc
     3181 aaataataaa aaacaaaaag ttctttgctg ataacgggca taaagatttt gaaaggttag
     3241 agtattacct tagctcggca attagttctc ttgtcgagga taaggtaaca gaaggcatta
     3301 aagacattaa cacggatgag aaccttgttt ttaaaaaaat aaatgaattg agtgacaagg
     3361 aaaaattaat tttagcagag tgcattatta aacattttga atattcaaat gttagaggaa
     3421 tgcaaaaggc atttaatatt tttggggggt aatggatgag taaaacgaca gcacagcaga
     3481 tcgcagattt gcaaaataaa ctacaagcat taagaacaaa aaaagcaaga caggacagaa
     3541 gggaagaaac aagacaaaaa ataatcctgg gtgcagaagt ggctaaggtt ttaggtaaaa
     3601 aaccggagga aattaacaag gctttaattc ttgggttgtt aagtaaaata aatgaacttg
     3661 acgaagaaga aaaacaagaa ttcacacgat taggattaaa tattttagaa gagtggaaga
     3721 atgaaaatcg taaaccgtga acaataaaaa accagccgat cttggctggt tttttactgt
     3781 gaagggttgc caggcttgcc tggaggtctt aaaaaggctc agagaggctc tcagggctta
     3841 tgggtgggtt tgttgattgt tgggtacctg agtgcctctc agttcgagat acagggcccg
     3901 gatacgggga aaggggaaaa aatcccccca ggggtggagg gtttggttag gttaggggga
     3961 aaaactgctt agaagtcgtt acaggctgtt ttgaggca
//
