LOCUS       NZ_CP070462             4863 bp    DNA     circular CON 04-DEC-2023
DEFINITION  Klebsiella pneumoniae strain B28484 plasmid p3B28484, complete
            sequence.
ACCESSION   NZ_CP070462
VERSION     NZ_CP070462.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17915109
            Assembly: GCF_016925415.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Multidrug resistant Klebsiella pneumoniae_B28484_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 94.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/04/2023 04:42:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,410
            CDSs (total)                      :: 5,281
            Genes (coding)                    :: 5,136
            CDSs (with protein)               :: 5,136
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 62 of 145
            Pseudo Genes (incomplete)         :: 82 of 145
            Pseudo Genes (internal stop)      :: 23 of 145
            Pseudo Genes (multiple problems)  :: 19 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070462.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B28484"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p3B28484"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian Medical College"
     gene            complement(364..786)
                     /locus_tag="JT747_RS26885"
                     /old_locus_tag="JT747_26885"
     CDS             complement(364..786)
                     /locus_tag="JT747_RS26885"
                     /old_locus_tag="JT747_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(786..977)
                     /locus_tag="JT747_RS26890"
                     /old_locus_tag="JT747_26890"
     CDS             complement(786..977)
                     /locus_tag="JT747_RS26890"
                     /old_locus_tag="JT747_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045265536.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1393..1506
                     /locus_tag="JT747_RS26895"
                     /old_locus_tag="JT747_26895"
     CDS             1393..1506
                     /locus_tag="JT747_RS26895"
                     /old_locus_tag="JT747_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(1743..2111)
                     /locus_tag="JT747_RS26900"
                     /old_locus_tag="JT747_26900"
     CDS             complement(1743..2111)
                     /locus_tag="JT747_RS26900"
                     /old_locus_tag="JT747_26900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(2184..3482)
                     /locus_tag="JT747_RS26905"
                     /old_locus_tag="JT747_26905"
     CDS             complement(2184..3482)
                     /locus_tag="JT747_RS26905"
                     /old_locus_tag="JT747_26905"
                     /inference="COORDINATES: protein motif:HMM:NF024700.3"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_045265534.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     Typing          1099..1506
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 atcttcacta gagtcgtacc cagagtcgcc gagtctaaaa aacaaccaaa cggggagtcg
       61 tgtctagagt cgggtcgtta ttaacttatt gtttttatag tcctgagtcg ttaccgactc
      121 tatgacgact cgccggggac tctatgacga ctcgtcatag agtcggatct atataaataa
      181 aatttaaaaa acgaaatttc aggaaagtaa cccactgata ttaaatacaa atctgcaaaa
      241 tgttaatttt gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctgacg
      301 atttttggga aggttcagga actccccggg atggccggag aggaaaacgg aagcatgacg
      361 gggtcaggat tttttccaga atttccacca cggggaatgg ggtggtgaag aaggttcgtg
      421 cttgtgctcc agaagctgca tagcctgacg aagggaatca atatgctcat ctcgagcctt
      481 aacagcccct ttaagaccat cgttttcagc ttcaagtaaa gcgattctat gttgtaaatc
      541 atgtaaacca acgctgttaa cacctgtaga ttctgcgtca ctttttacat catattttac
      601 agtatcgatg acaccatcaa gagaaccaaa cactctaata agctctgaag tatcaataac
      661 tttaatagcc ttaccgtgct ttacaacaga ggtgtaactt aacttcccac tgttaatcat
      721 cttgtaaatt gtggggcgac taaccttaac aagccgtgcc gcttcactaa tagacactct
      781 ggccatcagt taccatcctg taattcatcc agtctcgagg acagtgtacg gaaaagatgc
      841 tccgcatcat cgtggagttt ctcacaaagg tcggcctcga catcgagagc aagctcgtta
      901 agcttctcca gcaggagcag agactgattc tggatgaatt tagccatgtt cagcgctgtt
      961 ttctgttgtt tcgtcataag aagcctcaga gcgtcagtgc tcagtgtaaa accagtctct
     1021 tttccgcttc ctcgctcact cagtcgctac gctccggtcg ttcggctgcg gcgagcggca
     1081 agaccattct tataaaagct cgagaaaacc gagcgatcaa ggtaggagga aaagaggagg
     1141 ccgcgcagcg gccgtttttc cataggctcc gcccccctga caagcatcac agaaacggac
     1201 gctcaaatca gtggtggcga aacccgacag gactataaag atacctatca tttctgatag
     1261 gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgcc attccgctgt
     1321 tatggccgcg tttatctcat tccacgccag acactcagtt ccgggtaggc agttcgctcc
     1381 aagctggact gtatgcacga accccccgtt cagtccgact gctgcgcctt atccggtaac
     1441 tatcgtcttg agtccaaccc ggaaagacat gcaaaagcgc cactggcagc agccactggt
     1501 aactgattta gaggagttag tcttgaagtt atgcgccggt taaggctaaa ctgaaagaac
     1561 aagttttggt tactgcgctc ctccaagcca gttaccttgg ttctaagagc gccaactctt
     1621 ggaaccttcg aaaaaaccgc ccgccaaggc ggttttttcg tttacagagc gagagattac
     1681 gacgagaaca cagtgatctc aagaagatct tgcctcaaca tacagtacaa tcgtaactaa
     1741 gcctatttcc tgctcttcca aaaccgatga tccttatcaa aaaaaactat ataaaacaca
     1801 ttatccttta aaaaaccagc aacccttctt ttctggcctc ctatattttt tataacaccc
     1861 cagttctcat caactgatat ttcatcaggg catttaaaat cattaactgc tgaatctgga
     1921 aatcgagtgt agttagtaaa aataccatca gagctgactt ttgttatccc attagctgta
     1981 agataaacaa gcttttcaat taaatctggc aaactgccgt caaccctcca agaatcaata
     2041 gactgaccaa tatcatcgac gttatgaaaa tatctaaaac taacagaaat aaatctatct
     2101 gtagttttca taatgctctt tgaggatttt gtttcctctt tcctcgaaga gctacgaaga
     2161 ccgcggcctc tctttgacat aaatcatccc tcaggcataa gtttttcaaa ttcatctaaa
     2221 ttatcaaaca atgggatagc atcaaatgca ccagataaat aatcatgccc atacgatgat
     2281 tttaaagttc gctgcttctc tttatactca accaaagaat agagatgact acttcctgat
     2341 tttatatctt tatcaacaaa ccaaatctgg tcacgtctaa aacaatcagt gttcattaga
     2401 ttaacatcct gaacagcagc tattatttgg ctaagcccct cgctttgcgc atggaatatt
     2461 ctaaacaaaa acttagacaa aagggtgtga aacttagaat caaactcatc aactaacaat
     2521 aagttaccgt ttatcatggc attgtagatt ggaccaagaa gataaactat ctttttagta
     2581 ccctcagact caaaatcaat aggtactgaa atctcaccgc tagctgtttt tttcactact
     2641 ctaagagaaa aaaccttctg gttttttgca aaattttgta ttgaactaaa taaatttttt
     2701 aaattatcat catctgtttt tatatttcca aatggaaaat caggtgcgac ctcatcgagg
     2761 taaatatcat taatctggaa tgctggaagt atttttttta gccaagcctt aaaatcctca
     2821 gactcattta ttaacttcat ggtaattgct gtaaacatcc cttcattaga accagaaagg
     2881 aatacgatat tgttaaacca attaactact cgttttgaat gctcaccatt aaaaccagca
     2941 acaaccgaaa caaatggaac atcctccctt gttttttgaa taaccccttc tttgataaaa
     3001 aggtttgact cactaaaagc cgccttattt acctcaatag aaagaccatc tctttcaaag
     3061 agaatggttt ctcttgattt tggtgtgtag tacaaccatt cttcttttat tttcccctga
     3121 aaaatagaca atccatatct atatttaacc ccatctgaaa taaaagtcat ttccatttca
     3181 gatggtttat ttggtccatc ctcatcaagc aaaaacggta ttacgttctt tgtaatgcta
     3241 ctttcaatag cttgcacgga atgcaaaaca atatctttca ttaaagaaat tgttttcatg
     3301 agattagact tcccgctagc gttagcccca aatatcagag cagatttcag tatccgaaca
     3361 tcactcactg aaaccacgtt agccaagttt atgtcgtctt tcgcggatga caccgccaac
     3421 atgctcatat tttgagcatc cataaaggaa aatgcgttag aaagactaaa attaaccatc
     3481 ataagaacac cccttacgcc atccttaaac aacaaaatga gtaaatattg acaaaatcag
     3541 gacttaagga tagcatacaa gcgttctaaa acaacaggtg cgtttaatag atagcacgtt
     3601 atggaacact tgttaaaaaa agctaaatgc agggggtcta tgagattttt tctataccaa
     3661 atggttcctt tagccgcagt tgtcagctca gcagtgctag ctttacactc ggtttcagga
     3721 tggggatggt ttctctttgt agcatttatg ctcagcggag ctgatacagt gaagaaacat
     3781 ctaggaaatg acgcatgaaa ggagaaaaac gacgtttttt gaagtaagtg ggttagcctg
     3841 taccaccaga actaacccgt aacaaaccat acacaaatgc ggcacttgag taccacctca
     3901 agagcttgat aaagccagcc acaagatttg ttggttgcta cagtacaatt ttgatagcaa
     3961 aaatcaacac tcatgtggcg aaaaatagga aatgcaccat gcagacagac gagtctcagg
     4021 catacatttt tcgcccttat atcacagtaa agggcaaacg gatcacccgt ccaaacggtg
     4081 ggatgttcaa aattccaatt aatcgtgaaa agaagaagta agttgtgact gcagccctaa
     4141 cgggctgccc cacatttgat taaagaggat ggttctatgt cagtttactg tgtctcttat
     4201 gacctgaatc aagcaggtca aaactacaat gcactatatg atgagttaaa aaaatcgccg
     4261 gggtggtgcc accctttaga ttccacatgg ctaatatcaa ccggagaaac agcgcaacaa
     4321 ctgtcggatc gtttgcgtag gcatctggac aataatgaca ccttgcttgt gattggtgta
     4381 accaaagaat acgcaggatg gctcacccaa gacacatggg attggatgag aacagatata
     4441 tagatataaa gccctgcggg gcttttttta cgtctgtagc tggtgtggat ttaacataac
     4501 cccctaattg tacgcaccgc taaaacggcc tcagggcgtt tctgacggga aaccggtaaa
     4561 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     4621 catgagtatg cacgcaagtg catagcgcga aaattcacgc cattcagcca gggtaagtcc
     4681 tgtttccggc gttcttccca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     4741 gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc acgttcagga atcgggttcg
     4801 tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg
     4861 cga
//
