LOCUS       NZ_CP070453             4863 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B18064 plasmid p4B18064, complete
            sequence.
ACCESSION   NZ_CP070453
VERSION     NZ_CP070453.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17915099
            Assembly: GCF_016925375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Multidrug resistant Klebsiella pneumoniae_B18064_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2015
            Assembly Method                   :: Canu v. 1.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 79.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:55:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,411
            CDSs (total)                      :: 5,282
            Genes (coding)                    :: 5,141
            CDSs (with protein)               :: 5,141
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 61 of 141
            Pseudo Genes (incomplete)         :: 80 of 141
            Pseudo Genes (internal stop)      :: 23 of 141
            Pseudo Genes (multiple problems)  :: 19 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070453.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B18064"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4B18064"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2015"
                     /collected_by="Christian Medical College"
     gene            1081..2379
                     /locus_tag="JT745_RS26915"
                     /old_locus_tag="JT745_26915"
     CDS             1081..2379
                     /locus_tag="JT745_RS26915"
                     /old_locus_tag="JT745_26915"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_045265534.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     gene            2452..2820
                     /locus_tag="JT745_RS26920"
                     /old_locus_tag="JT745_26920"
     CDS             2452..2820
                     /locus_tag="JT745_RS26920"
                     /old_locus_tag="JT745_26920"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(3057..3170)
                     /locus_tag="JT745_RS26925"
                     /old_locus_tag="JT745_26925"
     CDS             complement(3057..3170)
                     /locus_tag="JT745_RS26925"
                     /old_locus_tag="JT745_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            3586..3777
                     /locus_tag="JT745_RS26930"
                     /old_locus_tag="JT745_26930"
     CDS             3586..3777
                     /locus_tag="JT745_RS26930"
                     /old_locus_tag="JT745_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045265536.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3777..4199
                     /locus_tag="JT745_RS26935"
                     /old_locus_tag="JT745_26935"
     CDS             3777..4199
                     /locus_tag="JT745_RS26935"
                     /old_locus_tag="JT745_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(3058..3465)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 gttttaccgg tttcccgtca gaaacgccct gaggccgttt tagcggtgcg tacaattagg
       61 gggttatgtt aaatccacac cagctacaga cgtaaaaaaa gccccgcagg gctttatatc
      121 tatatatctg ttctcatcca atcccatgtg tcttgggtga gccatcctgc gtattctttg
      181 gttacaccaa tcacaagcaa ggtgtcatta ttgtccagat gcctacgcaa acgatccgac
      241 agttgttgcg ctgtttctcc ggttgatatt agccatgtgg aatctaaagg gtggcaccac
      301 cccggcgatt tttttaactc atcatatagt gcattgtagt tttgacctgc ttgattcagg
      361 tcataagaga cacagtaaac tgacatagaa ccatcctctt taatcaaatg tggggcagcc
      421 cgttagggct gcagtcacaa cttacttctt cttttcacga ttaattggaa ttttgaacat
      481 cccaccgttt ggacgggtga tccgtttgcc ctttactgtg atataagggc gaaaaatgta
      541 tgcctgagac tcgtctgtct gcatggtgca tttcctattt ttcgccacat gagtgttgat
      601 ttttgctatc aaaattgtac tgtagcaacc aacaaatctt gtggctggct ttatcaagct
      661 cttgaggtgg tactcaagtg ccgcatttgt gtatggtttg ttacgggtta gttctggtgg
      721 tacaggctaa cccacttact tcaaaaaacg tcgtttttct cctttcatgc gtcatttcct
      781 agatgtttct tcactgtatc agctccgctg agcataaatg ctacaaagag aaaccatccc
      841 catcctgaaa ccgagtgtaa agctagcact gctgagctga caactgcggc taaaggaacc
      901 atttggtata gaaaaaatct catagacccc ctgcatttag ctttttttaa caagtgttcc
      961 ataacgtgct atctattaaa cgcacctgtt gttttagaac gcttgtatgc tatccttaag
     1021 tcctgatttt gtcaatattt actcattttg ttgtttaagg atggcgtaag gggtgttctt
     1081 atgatggtta attttagtct ttctaacgca ttttccttta tggatgctca aaatatgagc
     1141 atgttggcgg tgtcatccgc gaaagacgac ataaacttgg ctaacgtggt ttcagtgagt
     1201 gatgttcgga tactgaaatc tgctctgata tttggggcta acgctagcgg gaagtctaat
     1261 ctcatgaaaa caatttcttt aatgaaagat attgttttgc attccgtgca agctattgaa
     1321 agtagcatta caaagaacgt aataccgttt ttgcttgatg aggatggacc aaataaacca
     1381 tctgaaatgg aaatgacttt tatttcagat ggggttaaat atagatatgg attgtctatt
     1441 tttcagggga aaataaaaga agaatggttg tactacacac caaaatcaag agaaaccatt
     1501 ctctttgaaa gagatggtct ttctattgag gtaaataagg cggcttttag tgagtcaaac
     1561 ctttttatca aagaaggggt tattcaaaaa acaagggagg atgttccatt tgtttcggtt
     1621 gttgctggtt ttaatggtga gcattcaaaa cgagtagtta attggtttaa caatatcgta
     1681 ttcctttctg gttctaatga agggatgttt acagcaatta ccatgaagtt aataaatgag
     1741 tctgaggatt ttaaggcttg gctaaaaaaa atacttccag cattccagat taatgatatt
     1801 tacctcgatg aggtcgcacc tgattttcca tttggaaata taaaaacaga tgatgataat
     1861 ttaaaaaatt tatttagttc aatacaaaat tttgcaaaaa accagaaggt tttttctctt
     1921 agagtagtga aaaaaacagc tagcggtgag atttcagtac ctattgattt tgagtctgag
     1981 ggtactaaaa agatagttta tcttcttggt ccaatctaca atgccatgat aaacggtaac
     2041 ttattgttag ttgatgagtt tgattctaag tttcacaccc ttttgtctaa gtttttgttt
     2101 agaatattcc atgcgcaaag cgaggggctt agccaaataa tagctgctgt tcaggatgtt
     2161 aatctaatga acactgattg ttttagacgt gaccagattt ggtttgttga taaagatata
     2221 aaatcaggaa gtagtcatct ctattctttg gttgagtata aagagaagca gcgaacttta
     2281 aaatcatcgt atgggcatga ttatttatct ggtgcatttg atgctatccc attgtttgat
     2341 aatttagatg aatttgaaaa acttatgcct gagggatgat ttatgtcaaa gagaggccgc
     2401 ggtcttcgta gctcttcgag gaaagaggaa acaaaatcct caaagagcat tatgaaaact
     2461 acagatagat ttatttctgt tagttttaga tattttcata acgtcgatga tattggtcag
     2521 tctattgatt cttggagggt tgacggcagt ttgccagatt taattgaaaa gcttgtttat
     2581 cttacagcta atgggataac aaaagtcagc tctgatggta tttttactaa ctacactcga
     2641 tttccagatt cagcagttaa tgattttaaa tgccctgatg aaatatcagt tgatgagaac
     2701 tggggtgtta taaaaaatat aggaggccag aaaagaaggg ttgctggttt tttaaaggat
     2761 aatgtgtttt atatagtttt ttttgataag gatcatcggt tttggaagag caggaaatag
     2821 gcttagttac gattgtactg tatgttgagg caagatcttc ttgagatcac tgtgttctcg
     2881 tcgtaatctc tcgctctgta aacgaaaaaa ccgccttggc gggcggtttt ttcgaaggtt
     2941 ccaagagttg gcgctcttag aaccaaggta actggcttgg aggagcgcag taaccaaaac
     3001 ttgttctttc agtttagcct taaccggcgc ataacttcaa gactaactcc tctaaatcag
     3061 ttaccagtgg ctgctgccag tggcgctttt gcatgtcttt ccgggttgga ctcaagacga
     3121 tagttaccgg ataaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccagc
     3181 ttggagcgaa ctgcctaccc ggaactgagt gtctggcgtg gaatgagata aacgcggcca
     3241 taacagcgga atggcaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc
     3301 gcctatcaga aatgataggt atctttatag tcctgtcggg tttcgccacc actgatttga
     3361 gcgtccgttt ctgtgatgct tgtcaggggg gcggagccta tggaaaaacg gccgctgcgc
     3421 ggcctcctct tttcctccta ccttgatcgc tcggttttct cgagctttta taagaatggt
     3481 cttgccgctc gccgcagccg aacgaccgga gcgtagcgac tgagtgagcg aggaagcgga
     3541 aaagagactg gttttacact gagcactgac gctctgaggc ttcttatgac gaaacaacag
     3601 aaaacagcgc tgaacatggc taaattcatc cagaatcagt ctctgctcct gctggagaag
     3661 cttaacgagc ttgctctcga tgtcgaggcc gacctttgtg agaaactcca cgatgatgcg
     3721 gagcatcttt tccgtacact gtcctcgaga ctggatgaat tacaggatgg taactgatgg
     3781 ccagagtgtc tattagtgaa gcggcacggc ttgttaaggt tagtcgcccc acaatttaca
     3841 agatgattaa cagtgggaag ttaagttaca cctctgttgt aaagcacggt aaggctatta
     3901 aagttattga tacttcagag cttattagag tgtttggttc tcttgatggt gtcatcgata
     3961 ctgtaaaata tgatgtaaaa agtgacgcag aatctacagg tgttaacagc gttggtttac
     4021 atgatttaca acatagaatc gctttacttg aagctgaaaa cgatggtctt aaaggggctg
     4081 ttaaggctcg agatgagcat attgattccc ttcgtcaggc tatgcagctt ctggagcaca
     4141 agcacgaacc ttcttcacca ccccattccc cgtggtggaa attctggaaa aaatcctgac
     4201 cccgtcatgc ttccgttttc ctctccggcc atcccgggga gttcctgaac cttcccaaaa
     4261 atcgtcagat ctctgaacgc tcctcaccac taaaaatacc cacaccccac gcaaaattaa
     4321 cattttgcag atttgtattt aatatcagtg ggttactttc ctgaaatttc gttttttaaa
     4381 ttttatttat atagatccga ctctatgacg agtcgtcata gagtccccgg cgagtcgtca
     4441 tagagtcggt aacgactcag gactataaaa acaataagtt aataacgacc cgactctaga
     4501 cacgactccc cgtttggttg ttttttagac tcggcgactc tgggtacgac tctagtgaag
     4561 attcgccggt gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg attgccggcc
     4621 gtcgacgaac ccgatttctg aacgtgctga aaaccggttt tccggctgcg ccggcaacgc
     4681 ttcacggcca cgcaataaat tgcgtccccg ttcaccgttt tcggatggga agaacgccgg
     4741 aaacaggact taccctggct gaatggcgtg aattttcgcg ctatgcactt gcgtgcatac
     4801 tcatgcatgc cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg tggtttgttg
     4861 cct
//
