LOCUS       NZ_CP070434             3998 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B3923 plasmid p4B3923, complete
            sequence.
ACCESSION   NZ_CP070434
VERSION     NZ_CP070434.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17912169
            Assembly: GCF_016925295.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Multidrug resistant Klebsiella pneumoniae_B3923_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 115.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 07:52:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,409
            CDSs (total)                      :: 5,280
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 62 of 141
            Pseudo Genes (incomplete)         :: 80 of 141
            Pseudo Genes (internal stop)      :: 23 of 141
            Pseudo Genes (multiple problems)  :: 20 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070434.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B3923"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4B3923"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2015"
                     /collected_by="Christian Medical College"
     gene            complement(join(3320..3998,1..515))
                     /gene="mobQ"
                     /locus_tag="JT729_RS26910"
                     /old_locus_tag="JT729_26910"
     CDS             complement(join(3320..3998,1..515))
                     /gene="mobQ"
                     /locus_tag="JT729_RS26910"
                     /old_locus_tag="JT729_26910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_096645250.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQIEWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            759..1142
                     /locus_tag="JT729_RS26915"
                     /old_locus_tag="JT729_26915"
     CDS             759..1142
                     /locus_tag="JT729_RS26915"
                     /old_locus_tag="JT729_26915"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579763.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     gene            1146..1430
                     /locus_tag="JT729_RS26920"
                     /old_locus_tag="JT729_26920"
     CDS             1146..1430
                     /locus_tag="JT729_RS26920"
                     /old_locus_tag="JT729_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_071579762.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(1669..1932)
                     /locus_tag="JT729_RS26925"
                     /old_locus_tag="JT729_26925"
     CDS             complement(1669..1932)
                     /locus_tag="JT729_RS26925"
                     /old_locus_tag="JT729_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(1929..2783)
                     /locus_tag="JT729_RS26930"
                     /old_locus_tag="JT729_26930"
     CDS             complement(1929..2783)
                     /locus_tag="JT729_RS26930"
                     /old_locus_tag="JT729_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     Typing          complement(4..515)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.415"
                     /coverage="85.0"
                     /evalue="4.11e-112"
     Typing          complement(3504..3997)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="4.17e-90"
ORIGIN
        1 attatttccc agttaactcg ccaggcttca atgttttctc tattattcca ttctatttgt
       61 ttgttcccaa atccatttgg gctaacagtt cttaaagtta gcattacatg agcatgaggg
      121 ttatgactat caagatcatg aaatgctatg tctgcaatca ttccataatc cacgaaattt
      181 tcttggcagt aatcgaggac gatctgcttc ttctggccgt cgtccagctc cttggggatg
      241 gctatgtcca gataacggca taattcggca tcctttcgct tctcgccagc ctctacggca
      301 ttccagaggg tgcctgagtc cttcaccagt tcggcaggtg tgccggcagg ggagaggata
      361 gcagagccga aaaatccctc tttttttgaa taatcccagg ttgccccggt tcgattatct
      421 tttgtctttt ctcggttttg atatgcagat aaataaaccg aagatttacc ttctgatctt
      481 cgtaattttt taaagctgat atgaaatgtc gccatagtct accccttact ttttttgtct
      541 tttgaatttt ggtttttagc cccgcagggc ccatacgtcg caagcgacgt gtaagtgggc
      601 attactaata ttattttttc ggttgcacct caaaataata agtaatgaac cccaggcttc
      661 gcccaatatt aaatattatt tgttttattc taaaatatat ttgactaaag taaataatgg
      721 aggtaataaa taacaaaata aataattagg aggttattat gaataaactt ataacagagg
      781 cagaaggtgc aaaaatcgaa agtgttttta aatacatatt agaggcatct gaaagcatat
      841 taatagagca tgggcatgtt attacggggc aaataataaa aaacaaaaag ttctttgctg
      901 ataacgggca taaagatttt gaaaggttag agtattacct tagctcggca attagttctc
      961 ttgtcgagga taaggtaaca gaaggcatta aagacattaa cacggatgag aaccttgttt
     1021 ttaaaaaaat aaatgaattg agtgacaagg aaaaattaat tttagcagag tgcattatta
     1081 aacattttga atattcaaat gttagaggaa tgcaaaaggc atttaatatt tttggggggt
     1141 aatggatgag taaaacgaca gcacagcaga tcgcagattt gcaaaataaa ctacaagcat
     1201 taagaacaaa aaaagcaaga caggacagaa gggaagaaac aagacaaaaa ataatcctgg
     1261 gtgcagaagt ggctaaggtt ttaggtaaaa aaccggagga aattaacaag gctttaattc
     1321 ttgggttgtt aagtaaaata aatgaacttg acgaagaaga aaaacaagaa ttcacacgat
     1381 taggattaaa tattttagaa gagtggaaga atgaaaatcg taaaccgtga acaataaaaa
     1441 accagccgat cttggctggt tttttactgt gaagggttgc caggcttgcc tggaggtctt
     1501 aaaaaggctc agagaggctc tcagggctta tgggtgggtt tgttgattgt tgggtacctg
     1561 agtgcctctc agttcgagat acagggcccg gatacgggga aaggggaaaa aatcccccca
     1621 ggggtggagg gtttggttag gttaggggga aaaactgctt agaagtcgtt acaggctgtt
     1681 ttgaggcata tctaaggcaa cataacggcg atacagggca acggctccgt gatacgaaca
     1741 gcccattttg tcggctactt gttgccattt cagcccctgg cttcgtagtt cgtaagcctg
     1801 acgacgacga tccattgctg ttttttcgta atcgtcacga gattgagaaa aatattttct
     1861 tacagttctt tcactacgac ctgtcatttc cattatttct tttgctttta tttttttacc
     1921 attaatgatc ataaaacccc cttgctcatt aatatgtcta acatttcatt tttaacattg
     1981 gtttttcgag gttttttggc tctgtttgat tggatttgtg aaaatgtatg gctatcaaaa
     2041 tttttccaag tccaacgggc tacacttcta actgttgctt ttatctcttt gtattgtaac
     2101 gggttttgtg gattgcaaaa agtgtttaag ccctgtgcct ttaatagtag ttcgttttcg
     2161 aatttctcga aagtggatat ttttttgaaa tttaaaaccg ttctgtaagc atattgccgt
     2221 agatcttcaa acaaattaac attgcgacct aaaccataag cctcgctttt tttcttttgt
     2281 atttgaggct tttctaagtc cacaaaatca gctaaataat ccaggtcata aaggtcagca
     2341 cctgaccaga acggcgacca gtgatcattg agtgggtttt ttgtaatcaa tccagcaaac
     2401 cccagatctg cttttagttt tttcgccata gccatttcaa cggctgcggc atacttcaac
     2461 ggctttaacc ttgcttgttg tgtcttacaa accccggctt tcagcatata gccgtagtga
     2521 caacgacctg tttttggtgt ttttgtaata aagtgaggta tgggtaagcc cacctgctca
     2581 aatgtataga atgaattttt cttatctaga tcaaagagta agcaagtttg aatggctggc
     2641 tgatttgcct gtaaatacag catttcaagg gcttttgcct tgttccttat gcttaatccc
     2701 atatcgaggt tatttgtaca ataaggcttg ttaggcattt gatttttaaa aaaatcgatg
     2761 ttatgttctt cttgtattat cattttttca gtccttttta tctggcccta ctctctgttt
     2821 tctgtcgcca aaacaaaatc gcagagggtg gggtttttat ttttatagat ttttcaatcc
     2881 tgtaccagtt ctaaccgatc tcaaaaattt gtcaagtgtg cgtctaacca tatcagataa
     2941 cagcccaacg aagttggggc agttctttac atacggcagc aaagcattac tatcagatct
     3001 atggtgcgaa gcattaccga agatagaaaa acaataaaaa aagaaaaaag agtattgttc
     3061 gaagaaaaat tggcgattcg attttttttg gtgaatatat gaaaaaaaag aaagtgcaga
     3121 aaaaacggaa aagaaataag catagtaaat tttttaaaat gcttatgaag tcagttaatg
     3181 aagctgtggc aatacataaa ggggaaatag aaccggcgag gatcacaact ctaaaaatgt
     3241 agtatcttat agtatagtat taaataatat taagttgtat taagtgatac tactatatag
     3301 gaatatatac cactatatac tacattccaa atttcttctt cctcttaggt tgctgatctt
     3361 cgaattccgg cattggttcc ggctcaggct cttcaatttt tggttgtggt tggttcatag
     3421 gagacattcc agagcctgaa cccatttttc cgcctccatt atttccaccg gttccggttc
     3481 ctgtgcctgt tttaggtttt gtttctaact ctgtctttaa tatatgttcg ccaaattcat
     3541 ttattacata actactaacg aaattttgag gatcttcttt ttcttctttt ttatttttat
     3601 ttacattagg ttttgattta aatatatgct gggtcttttt gtataatgcc tttaaattat
     3661 caatgattga ccttgcaaga ggctttttct ctattttcat agtttcctct ggctgaggtg
     3721 gtttggtttg ctgtaagtat gttgcattag ctatttttaa taataatgct ttttcttttt
     3781 gctccatagc tcttaatttt tgaccttttc gccattggtg cctgtgtatc ctggtcattg
     3841 gcgttctgtc cagttcaata gtttttgaaa tccacttggc tttctctttc ggagttttag
     3901 caaaaacagc ctttctttct gcctcttctt tttggtcttt taatgaacgg gagtcaatac
     3961 gagatttatg accatatttt tttaatcggt catttgct
//
