LOCUS       NZ_CP070429             4863 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B3824 plasmid p4B3824, complete
            sequence.
ACCESSION   NZ_CP070429
VERSION     NZ_CP070429.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17911903
            Assembly: GCF_016925275.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Multidrug resistant Klebsiella pneumoniae_B3824_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 127.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:01:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,410
            CDSs (total)                      :: 5,281
            Genes (coding)                    :: 5,140
            CDSs (with protein)               :: 5,140
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 62 of 141
            Pseudo Genes (incomplete)         :: 80 of 141
            Pseudo Genes (internal stop)      :: 23 of 141
            Pseudo Genes (multiple problems)  :: 20 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070429.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B3824"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4B3824"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2015"
                     /collected_by="Christian Medical College"
     gene            complement(495..917)
                     /locus_tag="JT727_RS26910"
                     /old_locus_tag="JT727_26910"
     CDS             complement(495..917)
                     /locus_tag="JT727_RS26910"
                     /old_locus_tag="JT727_26910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_045265535.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(917..1108)
                     /locus_tag="JT727_RS26915"
                     /old_locus_tag="JT727_26915"
     CDS             complement(917..1108)
                     /locus_tag="JT727_RS26915"
                     /old_locus_tag="JT727_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_045265536.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELALDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1524..1637
                     /locus_tag="JT727_RS26920"
                     /old_locus_tag="JT727_26920"
     CDS             1524..1637
                     /locus_tag="JT727_RS26920"
                     /old_locus_tag="JT727_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032634168.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHAKAPLAAATGN"
                     /name="hypothetical protein"
     gene            complement(1874..2242)
                     /locus_tag="JT727_RS26925"
                     /old_locus_tag="JT727_26925"
     CDS             complement(1874..2242)
                     /locus_tag="JT727_RS26925"
                     /old_locus_tag="JT727_26925"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045626380.1"
                     /translation="MKTTDRFISVSFRYFHNVDDIGQSIDSWRVDGSLPDLIEKLVYLT
                     ANGITKVSSDGIFTNYTRFPDSAVNDFKCPDEISVDENWGVIKNIGGQKRRVAGFLKDN
                     VFYIVFFDKDHRFWKSRK"
                     /name="hypothetical protein"
     gene            complement(2315..3613)
                     /locus_tag="JT727_RS26930"
                     /old_locus_tag="JT727_26930"
     CDS             complement(2315..3613)
                     /locus_tag="JT727_RS26930"
                     /old_locus_tag="JT727_26930"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_045265534.1"
                     /translation="MMVNFSLSNAFSFMDAQNMSMLAVSSAKDDINLANVVSVSDVRIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVIPFLLDEDGPNKPSEME
                     MTFISDGVKYRYGLSIFQGKIKEEWLYYTPKSRETILFERDGLSIEVNKAAFSESNLFI
                     KEGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFNNIVFLSGSNEGMFTAITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPDFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTASGEISVPIDFESEGTKKIVYLLGPIYNAMINGNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGLSQIIAAVQDVNLMNTDCFRRDQIWFVDKDIKSGSSHLYSLVEYKEKQRT
                     LKSSYGHDYLSGAFDAIPLFDNLDEFEKLMPEG"
                     /name="ATP-binding protein"
     Typing          1230..1637
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.484"
                     /coverage="99.0"
                     /evalue="1.3e-157"
ORIGIN
        1 gcgtggccgt gaagcgttgc cggcgcagcc ggaaaaccgg ttttcagcac gttcagaaat
       61 cgggttcgtc gacggccggc aatcacgtta tggcaagtcg ccggacgact caccacacga
      121 ttcaccggcg aatcttcact agagtcgtac ccagagtcgc cgagtctaaa aaacaaccaa
      181 acggggagtc gtgtctagag tcgggtcgtt attaacttat tgtttttata gtcctgagtc
      241 gttaccgact ctatgacgac tcgccgggga ctctatgacg actcgtcata gagtcggatc
      301 tatataaata aaatttaaaa aacgaaattt caggaaagta acccactgat attaaataca
      361 aatctgcaaa atgttaattt tgcgtggggt gtgggtattt ttagtggtga ggagcgttca
      421 gagatctgac gatttttggg aaggttcagg aactccccgg gatggccgga gaggaaaacg
      481 gaagcatgac ggggtcagga ttttttccag aatttccacc acggggaatg gggtggtgaa
      541 gaaggttcgt gcttgtgctc cagaagctgc atagcctgac gaagggaatc aatatgctca
      601 tctcgagcct taacagcccc tttaagacca tcgttttcag cttcaagtaa agcgattcta
      661 tgttgtaaat catgtaaacc aacgctgtta acacctgtag attctgcgtc actttttaca
      721 tcatatttta cagtatcgat gacaccatca agagaaccaa acactctaat aagctctgaa
      781 gtatcaataa ctttaatagc cttaccgtgc tttacaacag aggtgtaact taacttccca
      841 ctgttaatca tcttgtaaat tgtggggcga ctaaccttaa caagccgtgc cgcttcacta
      901 atagacactc tggccatcag ttaccatcct gtaattcatc cagtctcgag gacagtgtac
      961 ggaaaagatg ctccgcatca tcgtggagtt tctcacaaag gtcggcctcg acatcgagag
     1021 caagctcgtt aagcttctcc agcaggagca gagactgatt ctggatgaat ttagccatgt
     1081 tcagcgctgt tttctgttgt ttcgtcataa gaagcctcag agcgtcagtg ctcagtgtaa
     1141 aaccagtctc ttttccgctt cctcgctcac tcagtcgcta cgctccggtc gttcggctgc
     1201 ggcgagcggc aagaccattc ttataaaagc tcgagaaaac cgagcgatca aggtaggagg
     1261 aaaagaggag gccgcgcagc ggccgttttt ccataggctc cgcccccctg acaagcatca
     1321 cagaaacgga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gatacctatc
     1381 atttctgata ggcggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgc
     1441 cattccgctg ttatggccgc gtttatctca ttccacgcca gacactcagt tccgggtagg
     1501 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct
     1561 tatccggtaa ctatcgtctt gagtccaacc cggaaagaca tgcaaaagcg ccactggcag
     1621 cagccactgg taactgattt agaggagtta gtcttgaagt tatgcgccgg ttaaggctaa
     1681 actgaaagaa caagttttgg ttactgcgct cctccaagcc agttaccttg gttctaagag
     1741 cgccaactct tggaaccttc gaaaaaaccg cccgccaagg cggttttttc gtttacagag
     1801 cgagagatta cgacgagaac acagtgatct caagaagatc ttgcctcaac atacagtaca
     1861 atcgtaacta agcctatttc ctgctcttcc aaaaccgatg atccttatca aaaaaaacta
     1921 tataaaacac attatccttt aaaaaaccag caacccttct tttctggcct cctatatttt
     1981 ttataacacc ccagttctca tcaactgata tttcatcagg gcatttaaaa tcattaactg
     2041 ctgaatctgg aaatcgagtg tagttagtaa aaataccatc agagctgact tttgttatcc
     2101 cattagctgt aagataaaca agcttttcaa ttaaatctgg caaactgccg tcaaccctcc
     2161 aagaatcaat agactgacca atatcatcga cgttatgaaa atatctaaaa ctaacagaaa
     2221 taaatctatc tgtagttttc ataatgctct ttgaggattt tgtttcctct ttcctcgaag
     2281 agctacgaag accgcggcct ctctttgaca taaatcatcc ctcaggcata agtttttcaa
     2341 attcatctaa attatcaaac aatgggatag catcaaatgc accagataaa taatcatgcc
     2401 catacgatga ttttaaagtt cgctgcttct ctttatactc aaccaaagaa tagagatgac
     2461 tacttcctga ttttatatct ttatcaacaa accaaatctg gtcacgtcta aaacaatcag
     2521 tgttcattag attaacatcc tgaacagcag ctattatttg gctaagcccc tcgctttgcg
     2581 catggaatat tctaaacaaa aacttagaca aaagggtgtg aaacttagaa tcaaactcat
     2641 caactaacaa taagttaccg tttatcatgg cattgtagat tggaccaaga agataaacta
     2701 tctttttagt accctcagac tcaaaatcaa taggtactga aatctcaccg ctagctgttt
     2761 ttttcactac tctaagagaa aaaaccttct ggttttttgc aaaattttgt attgaactaa
     2821 ataaattttt taaattatca tcatctgttt ttatatttcc aaatggaaaa tcaggtgcga
     2881 cctcatcgag gtaaatatca ttaatctgga atgctggaag tatttttttt agccaagcct
     2941 taaaatcctc agactcattt attaacttca tggtaattgc tgtaaacatc ccttcattag
     3001 aaccagaaag gaatacgata ttgttaaacc aattaactac tcgttttgaa tgctcaccat
     3061 taaaaccagc aacaaccgaa acaaatggaa catcctccct tgttttttga ataacccctt
     3121 ctttgataaa aaggtttgac tcactaaaag ccgccttatt tacctcaata gaaagaccat
     3181 ctctttcaaa gagaatggtt tctcttgatt ttggtgtgta gtacaaccat tcttctttta
     3241 ttttcccctg aaaaatagac aatccatatc tatatttaac cccatctgaa ataaaagtca
     3301 tttccatttc agatggttta tttggtccat cctcatcaag caaaaacggt attacgttct
     3361 ttgtaatgct actttcaata gcttgcacgg aatgcaaaac aatatctttc attaaagaaa
     3421 ttgttttcat gagattagac ttcccgctag cgttagcccc aaatatcaga gcagatttca
     3481 gtatccgaac atcactcact gaaaccacgt tagccaagtt tatgtcgtct ttcgcggatg
     3541 acaccgccaa catgctcata ttttgagcat ccataaagga aaatgcgtta gaaagactaa
     3601 aattaaccat cataagaaca ccccttacgc catccttaaa caacaaaatg agtaaatatt
     3661 gacaaaatca ggacttaagg atagcataca agcgttctaa aacaacaggt gcgtttaata
     3721 gatagcacgt tatggaacac ttgttaaaaa aagctaaatg cagggggtct atgagatttt
     3781 ttctatacca aatggttcct ttagccgcag ttgtcagctc agcagtgcta gctttacact
     3841 cggtttcagg atggggatgg tttctctttg tagcatttat gctcagcgga gctgatacag
     3901 tgaagaaaca tctaggaaat gacgcatgaa aggagaaaaa cgacgttttt tgaagtaagt
     3961 gggttagcct gtaccaccag aactaacccg taacaaacca tacacaaatg cggcacttga
     4021 gtaccacctc aagagcttga taaagccagc cacaagattt gttggttgct acagtacaat
     4081 tttgatagca aaaatcaaca ctcatgtggc gaaaaatagg aaatgcacca tgcagacaga
     4141 cgagtctcag gcatacattt ttcgccctta tatcacagta aagggcaaac ggatcacccg
     4201 tccaaacggt gggatgttca aaattccaat taatcgtgaa aagaagaagt aagttgtgac
     4261 tgcagcccta acgggctgcc ccacatttga ttaaagagga tggttctatg tcagtttact
     4321 gtgtctctta tgacctgaat caagcaggtc aaaactacaa tgcactatat gatgagttaa
     4381 aaaaatcgcc ggggtggtgc caccctttag attccacatg gctaatatca accggagaaa
     4441 cagcgcaaca actgtcggat cgtttgcgta ggcatctgga caataatgac accttgcttg
     4501 tgattggtgt aaccaaagaa tacgcaggat ggctcaccca agacacatgg gattggatga
     4561 gaacagatat atagatataa agccctgcgg ggcttttttt acgtctgtag ctggtgtgga
     4621 tttaacataa ccccctaatt gtacgcaccg ctaaaacggc ctcagggcgt ttctgacggg
     4681 aaaccggtaa aacaggcaac aaaccacccg aaaacccgcc agaaacgcgc aggctctgtt
     4741 tttacggcat gcatgagtat gcacgcaagt gcatagcgcg aaaattcacg ccattcagcc
     4801 agggtaagtc ctgtttccgg cgttcttccc atccgaaaac ggtgaacggg gacgcaattt
     4861 att
//
