LOCUS       NZ_CP070423             4991 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella quasipneumoniae strain B2663 plasmid p3B2663, complete
            sequence.
ACCESSION   NZ_CP070423
VERSION     NZ_CP070423.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17911902
            Assembly: GCF_016925255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Multidrug resistant Klebsiella pneumoniae_B2663_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 101.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:03:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,462
            CDSs (total)                      :: 5,334
            Genes (coding)                    :: 5,210
            CDSs (with protein)               :: 5,210
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 124
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 124
            Pseudo Genes (frameshifted)       :: 53 of 124
            Pseudo Genes (incomplete)         :: 70 of 124
            Pseudo Genes (internal stop)      :: 31 of 124
            Pseudo Genes (multiple problems)  :: 25 of 124
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070423.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Klebsiella quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B2663"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:1463165"
                     /plasmid="p3B2663"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2015"
                     /collected_by="Christian Medical College"
     gene            complement(join(4829..4991,1..29))
                     /locus_tag="JT726_RS27155"
                     /old_locus_tag="JT726_27155"
     CDS             complement(join(4829..4991,1..29))
                     /locus_tag="JT726_RS27155"
                     /old_locus_tag="JT726_27155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032489493.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            445..611
                     /locus_tag="JT726_RS27525"
                     /pseudo=""
     CDS             445..611
                     /locus_tag="JT726_RS27525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            941..1999
                     /locus_tag="JT726_RS27160"
                     /old_locus_tag="JT726_27160"
     CDS             941..1999
                     /locus_tag="JT726_RS27160"
                     /old_locus_tag="JT726_27160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508929.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="WP_042109829.1"
                     /translation="MAIYAVWNNKGGVGKSYLTFQLASEYARQNPTKKVLVVDLCPQAN
                     SSSMLLGGMINGENTLNQIHSAIQRKTISGYIEDRIRSPYIPTNTGSSYVINVSTHNSK
                     VPNNLYLVTGDEQLELQASRVVGASFPGPHDAWRTVHLWINDLISDICNSWNNEDSCVF
                     IDCNPSFSIYTELALTAAERLLIPFSADGSSKRAVKAVLSLVYGERRHQNEPQSDFYLN
                     SNRYSMSLPQIYMYVGNRLTQMNNSSASAFKTVVTEIGDEIWKVWQTKPSLFCIHPNGS
                     STPNNKRSFRNMFQYEVNDANTASVVSGALGIPIATLTAGNKTVSGKIIKVNQTQLDKQ
                     VPNIVALAGMIE"
                     /name="ParA family protein"
     gene            complement(2029..2286)
                     /locus_tag="JT726_RS27165"
                     /old_locus_tag="JT726_27165"
     CDS             complement(2029..2286)
                     /locus_tag="JT726_RS27165"
                     /old_locus_tag="JT726_27165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_042109830.1"
                     /translation="MGSNNNQNKDLQDKQADLLDQTYTLGWMDGYAEGYDDAIEDTQNA
                     LNKLENENELLTEVVESKDNVIKELASSAGRALQAVKKNK"
                     /name="hypothetical protein"
     gene            2704..3135
                     /locus_tag="JT726_RS27170"
                     /old_locus_tag="JT726_27170"
     CDS             2704..3135
                     /locus_tag="JT726_RS27170"
                     /old_locus_tag="JT726_27170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_042109831.1"
                     /translation="MKIPAHLKHKPIIQVENYDRIDGPHADDTDAMGLSVGIAQWNTPG
                     WTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICITMDYSKNGRLSSSDDFPVTRST
                     DNPELAHHTEFMKKELKQNKSNLDVSLKRLSTELKRLGY"
                     /name="DUF6530 family protein"
     gene            complement(3229..3633)
                     /locus_tag="JT726_RS27175"
                     /old_locus_tag="JT726_27175"
     CDS             complement(3229..3633)
                     /locus_tag="JT726_RS27175"
                     /old_locus_tag="JT726_27175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="WP_008321396.1"
                     /translation="MTILVTIQAQLVVGEEQVIKSLAPEGMLAAVFEDDGRTGYFYALD
                     ESVEGNPIMDAVHIYNAEDVSDGHIPSDVKIGWSEDSQKCVLLINGYPHGAFDFVGKNG
                     YCRSGYPPPINKVWSLSGHEWSDSVDDFFR"
                     /name="DUF2251 domain-containing protein"
     gene            complement(4428..4829)
                     /locus_tag="JT726_RS27180"
                     /old_locus_tag="JT726_27180"
     CDS             complement(4428..4829)
                     /locus_tag="JT726_RS27180"
                     /old_locus_tag="JT726_27180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_032489494.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQLKGEHHSGQVSEQRDTASVDQCEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          151..553
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.857"
                     /coverage="99.0"
                     /evalue="7.72e-165"
ORIGIN
        1 ttcagcgctg ttttctgttg tttcgtcata agaggcctca gagcgtcagt gctcagtgta
       61 gggatgttct cttttccgct tcctcgctca ctcagtcgct acgctcctgc cgttcggctg
      121 cggcgagcgg caggagcatg cgtatgaaag ctcgagaaaa ccgagcgatc acgtaattta
      181 gaaaagggaa ggccgcacag cggccgtttt tccataggct ccgcccccct gacaagcatc
      241 acagaaacgg acgctcaaat cagtggtggc gaaacccgac aggactataa agatatcagg
      301 cgtttccccc tgatggctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg
      361 tcattccgct gttatggccg cgtttgtctc attccacgcc tgacactcag ttccgggtag
      421 gcagttcgct ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ctgctgcgcc
      481 tgttccggta actttcgact tgagtccaac ccggaaggca cgacaaaacg ccactggcag
      541 cagccactgg taaggggtgt acaagaatta ctggtgcaga gttcttgaag ttacagccta
      601 actacagcta aactgaaagg acagatttgg tggcagcggt cttgtacacc ctgttaccac
      661 ggttcagaag ttccccaact tactgatcct tcgaaaaacc acctccccag gtggtttttt
      721 cgtttacaga gcaaaagatt acatcccgca ggttaggatc tcaagaagat ccttttaatc
      781 ggaattcgac aactccagat aagtcatggt gagccagcca accccttcga ctgaatatcg
      841 ttccaaaaaa aggcagtcag ctgaaagaaa ctaacagtag aacaataatc tctagtaccc
      901 tgaatctaag ctaacaacaa aactagatta ggagttatag atggcgatct atgccgtatg
      961 gaataacaag ggtggtgtag ggaaaagtta ccttactttt cagttagcct ctgaatatgc
     1021 cagacagaac cctactaaaa aggtcctggt ggttgacctg tgcccacaag cgaactcgtc
     1081 ctctatgtta cttggtggaa tgataaatgg cgaaaacaca ctaaaccaga ttcattcagc
     1141 aatacagaga aagacaatat ctggatatat tgaggatagg atcagaagtc catatattcc
     1201 taccaacact ggttctagct atgtaataaa tgtatctaca cacaatagta aagttccaaa
     1261 taatctatat cttgtcactg gcgatgaaca attagaactc caagcatcaa gagttgttgg
     1321 ggcatcattt ccaggtcccc atgatgcatg gcgtactgtt cacctctgga ttaatgattt
     1381 aatcagtgac atttgcaact catggaataa tgaagatagc tgcgtattca ttgactgcaa
     1441 ccctagcttc tctatttata cagagttagc tttaaccgca gcagagcgcc ttttaattcc
     1501 tttctctgcc gatggttcat caaagagagc agttaaggct gtactttctt tagtttatgg
     1561 tgagcgtcgc catcagaatg aaccacaatc agatttctat cttaatagca atcgttattc
     1621 tatgtcgcta ccacaaatat atatgtatgt tggaaataga ttaacgcaaa tgaacaattc
     1681 atctgccagt gcatttaaga cggtagtaac cgaaattggt gatgaaattt ggaaagtatg
     1741 gcaaacaaaa ccaagtctgt tttgtattca cccaaatggg agctcaacgc caaataataa
     1801 aagatctttc aggaatatgt ttcagtatga agtaaatgat gcaaacacag catctgtcgt
     1861 atctggagcc ttaggtattc caatcgcgac attaactgca ggaaataaaa ctgtttcagg
     1921 aaaaataata aaggttaatc agacgcagct agataaacaa gtccccaaca ttgtagctct
     1981 cgctggtatg atcgaataaa atagcaaggc ccttaaaagg gccttttttt atttgttttt
     2041 ctttactgct tgcagcgccc tgccagcact agaggccaac tctttgatta cattatcttt
     2101 actttcaaca acctcagtaa gcaactcgtt ttcgttttcc agtttattca aagcgttctg
     2161 agtatcttca atagcatcat catacccttc ggcatagcca tccatccaac caagagtata
     2221 cgtttggtcc aaaagatcag cttgcttatc ctgcagatct ttattctgat tattgttaga
     2281 gcccatgttt tttcctcatt tgttggcgca tctgagtttt caaaccagga ggaattccat
     2341 ttctaagttt ctcacttagt ctgactggtg gtgtgtttcc tgagactgga ttacagtcag
     2401 catccacccc gacatctttc tttttatagt cgccaatatt cctaacgtta tctgtagtca
     2461 tagaaaacca cgcccattgc tgtgaataac cgaactataa accagaaaaa aaacgtacgg
     2521 cttatcataa cacttgacac aaaaaaacac cgctactctc caagcaaaaa acataaaatt
     2581 ggaaaaacaa aatcacaagc acatgataac aatagatatt aatatttact tacatttagt
     2641 gagttttata attagaaaaa caatgtaatt taataatcac aaacattacc taaggacgaa
     2701 ctgatgaaaa tacccgcgca tttgaagcat aaaccaatta ttcaagtcga aaattacgac
     2761 cgcattgacg gcccacatgc cgatgacact gatgctatgg ggctatctgt aggtattgct
     2821 caatggaaca ctcctggatg gactgagctt tcggcaaagg tttggcgaaa cacaggagaa
     2881 aaatggtcaa gacaatcaga agagctgcct cttcaccggg taatcgacct tgcaactctg
     2941 atctgtatca ccatggacta cagtaaaaat gggcgacttt catccagtga tgattttccc
     3001 gtgactcgct caactgataa ccctgagcta gcacatcaca ctgagtttat gaaaaaagag
     3061 ttaaaacaaa ataaaagcaa tcttgatgta agcctcaaac gcctctcgac tgaattgaag
     3121 agacttggct attaaaacta gctttagaaa tgtctaccta cttcacagac gaatttacca
     3181 taacatccgt ttttcgaacc ataatggcag gctatagcct gccgtaattc accggaaaaa
     3241 atcatccaca gagtcgctcc actcatgccc ggaaagagac cacaccttgt ttataggggg
     3301 aggataacca ctcctgcagt acccgttttt tcccacgaaa tcgaatgccc cgtgcgggta
     3361 tccattgatc aggagtacgc acttctgact gtcttctgac cagccaattt taacatcaga
     3421 aggaatgtga ccgtctgaaa cgtcttcggc attgtagatg tgtactgcgt ccataatcgg
     3481 gtttccctcg acggactcat cgagcgcata gaaatacccg gtccggccat cgtcttcgaa
     3541 cacggcggcc agcatgccct caggagcgag gctctttata acctgttcct ccccgacaac
     3601 gagctgggcc tgtatcgtca ctaaaatggt catcaggtct gttctccttt ccttggttgc
     3661 ttcggcagcc agctttcaga caagcggata atttaccata acatccgtta tcagcatcgt
     3721 cagaaacgcg ctgagggcgt ttctgacggg aaaccggtaa aacaggcaac aaaccacccg
     3781 aaaacccgcc agaaacgcgc aggctctgtt tttatggcat gcatgagtat gcacgcaagt
     3841 gcatagcgcg aatattcacg ccattcagac agggtaagtc ctgtttccgg cgttcttccc
     3901 atccgaaaac ggtgaacggg gacgcaattt attgcgtggc cgtgaagcgt tgccggcgca
     3961 gccggaaaac cggttttcag cacgttcagg aatcgggttc gtcgacggcc ggcaatcacg
     4021 ttatggcaag tcgccggacg actcaccaca cgattcaccg gcgactctgc actagagtcg
     4081 tacctagagt cgccgcgact aaaaaacaac caaacgggga gtcgggtcta gagtcgggtc
     4141 gttattaact cattgttttt atagccctga gtcgttaccg actctatgac gactcgccgg
     4201 ggactctatg acgagtcgtc atagagtcgg atctatataa ataaaattta aaaaacgaaa
     4261 tctcaggaaa gtaaactact gatattaaat acaaatctgc aaaattttaa ttttgcgtgg
     4321 ggtgtgggta tttttagtgg tgaggagcgt tcagagatct gactattttt gggaaggttc
     4381 aggaactccc cgggatggcc ggagaggaaa actgaagcat gacagggtca ggattttttc
     4441 cagaatttcc accatggcgc cggggattca gttaattttg cctggtgatt ttcaagaagg
     4501 agcatggcct gtttcaggct gtcaatgtgc gaatctttgg acaaaagtaa agtgttcagg
     4561 tgttcaatct cctgtttcag atgacgaatc acctgttcac actggtcaac agaggctgta
     4621 tcgcgctgtt cagagacctg tccagaatgg tgttcaccct tcagttgttc aaagggacca
     4681 aagacacgta aaagctctga agtatctacc atttttgcat tgcgctcacc gctacaggtg
     4741 gacaggtcac ctgctttaat taacctgtgc agtgttgttc tactcttacc cgtcagtcgg
     4801 gctgcctcac taatagacac tctggccatc agttaccatc ctgtaatgca tccagtctcg
     4861 aggacagtgt acggaaaaga tgctccgcat catcgtggag tttctcacaa aggtcggcct
     4921 cgacatcaag atcaagctcg ttaagcttct ccagcaggag cagagattga tgctggatga
     4981 atttagccat g
//
