LOCUS       NZ_CP070419             3998 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Klebsiella pneumoniae strain B1347 plasmid p4B1247, complete
            sequence.
ACCESSION   NZ_CP070419
VERSION     NZ_CP070419.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17911845
            Assembly: GCF_016925235.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Multidrug resistant Klebsiella pneumoniae_B1347_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 72.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 08:03:28
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,409
            CDSs (total)                      :: 5,280
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 62 of 141
            Pseudo Genes (incomplete)         :: 80 of 141
            Pseudo Genes (internal stop)      :: 23 of 141
            Pseudo Genes (multiple problems)  :: 20 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070419.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B1347"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4B1247"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2021"
                     /collected_by="Christian Medical College"
     gene            join(3242..3998,1..437)
                     /gene="mobQ"
                     /locus_tag="JT725_RS26910"
                     /old_locus_tag="JT725_26910"
     CDS             join(3242..3998,1..437)
                     /gene="mobQ"
                     /locus_tag="JT725_RS26910"
                     /old_locus_tag="JT725_26910"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_096645250.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQIEWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            974..1828
                     /locus_tag="JT725_RS26915"
                     /old_locus_tag="JT725_26915"
     CDS             974..1828
                     /locus_tag="JT725_RS26915"
                     /old_locus_tag="JT725_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     gene            1825..2088
                     /locus_tag="JT725_RS26920"
                     /old_locus_tag="JT725_26920"
     CDS             1825..2088
                     /locus_tag="JT725_RS26920"
                     /old_locus_tag="JT725_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(2327..2611)
                     /locus_tag="JT725_RS26925"
                     /old_locus_tag="JT725_26925"
     CDS             complement(2327..2611)
                     /locus_tag="JT725_RS26925"
                     /old_locus_tag="JT725_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_071579762.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(2615..2998)
                     /locus_tag="JT725_RS26930"
                     /old_locus_tag="JT725_26930"
     CDS             complement(2615..2998)
                     /locus_tag="JT725_RS26930"
                     /old_locus_tag="JT725_26930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579763.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     Typing          3243..3997
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.603"
                     /coverage="85.0"
                     /evalue="2.87e-173"
ORIGIN
        1 aaatagctaa tgcaacatac ttacagcaaa ccaaaccacc tcagccagag gaaactatga
       61 aaatagagaa aaagcctctt gcaaggtcaa tcattgataa tttaaaggca ttatacaaaa
      121 agacccagca tatatttaaa tcaaaaccta atgtaaataa aaataaaaaa gaagaaaaag
      181 aagatcctca aaatttcgtt agtagttatg taataaatga atttggcgaa catatattaa
      241 agacagagtt agaaacaaaa cctaaaacag gcacaggaac cggaaccggt ggaaataatg
      301 gaggcggaaa aatgggttca ggctctggaa tgtctcctat gaaccaacca caaccaaaaa
      361 ttgaagagcc tgagccggaa ccaatgccgg aattcgaaga tcagcaacct aagaggaaga
      421 agaaatttgg aatgtagtat atagtggtat atattcctat atagtagtat cacttaatac
      481 aacttaatat tatttaatac tatactataa gatactacat ttttagagtt gtgatcctcg
      541 ccggttctat ttccccttta tgtattgcca cagcttcatt aactgacttc ataagcattt
      601 taaaaaattt actatgctta tttcttttcc gttttttctg cactttcttt tttttcatat
      661 attcaccaaa aaaaatcgaa tcgccaattt ttcttcgaac aatactcttt tttctttttt
      721 tattgttttt ctatcttcgg taatgcttcg caccatagat ctgatagtaa tgctttgctg
      781 ccgtatgtaa agaactgccc caacttcgtt gggctgttat ctgatatggt tagacgcaca
      841 cttgacaaat ttttgagatc ggttagaact ggtacaggat tgaaaaatct ataaaaataa
      901 aaaccccacc ctctgcgatt ttgttttggc gacagaaaac agagagtagg gccagataaa
      961 aaggactgaa aaaatgataa tacaagaaga acataacatc gattttttta aaaatcaaat
     1021 gcctaacaag ccttattgta caaataacct cgatatggga ttaagcataa ggaacaaggc
     1081 aaaagccctt gaaatgctgt atttacaggc aaatcagcca gccattcaaa cttgcttact
     1141 ctttgatcta gataagaaaa attcattcta tacatttgag caggtgggct tacccatacc
     1201 tcactttatt acaaaaacac caaaaacagg tcgttgtcac tacggctata tgctgaaagc
     1261 cggggtttgt aagacacaac aagcaaggtt aaagccgttg aagtatgccg cagccgttga
     1321 aatggctatg gcgaaaaaac taaaagcaga tctggggttt gctggattga ttacaaaaaa
     1381 cccactcaat gatcactggt cgccgttctg gtcaggtgct gacctttatg acctggatta
     1441 tttagctgat tttgtggact tagaaaagcc tcaaatacaa aagaaaaaaa gcgaggctta
     1501 tggtttaggt cgcaatgtta atttgtttga agatctacgg caatatgctt acagaacggt
     1561 tttaaatttc aaaaaaatat ccactttcga gaaattcgaa aacgaactac tattaaaggc
     1621 acagggctta aacacttttt gcaatccaca aaacccgtta caatacaaag agataaaagc
     1681 aacagttaga agtgtagccc gttggacttg gaaaaatttt gatagccata cattttcaca
     1741 aatccaatca aacagagcca aaaaacctcg aaaaaccaat gttaaaaatg aaatgttaga
     1801 catattaatg agcaaggggg ttttatgatc attaatggta aaaaaataaa agcaaaagaa
     1861 ataatggaaa tgacaggtcg tagtgaaaga actgtaagaa aatatttttc tcaatctcgt
     1921 gacgattacg aaaaaacagc aatggatcgt cgtcgtcagg cttacgaact acgaagccag
     1981 gggctgaaat ggcaacaagt agccgacaaa atgggctgtt cgtatcacgg agccgttgcc
     2041 ctgtatcgcc gttatgttgc cttagatatg cctcaaaaca gcctgtaacg acttctaagc
     2101 agtttttccc cctaacctaa ccaaaccctc cacccctggg gggatttttt cccctttccc
     2161 cgtatccggg ccctgtatct cgaactgaga ggcactcagg tacccaacaa tcaacaaacc
     2221 cacccataag ccctgagagc ctctctgagc ctttttaaga cctccaggca agcctggcaa
     2281 cccttcacag taaaaaacca gccaagatcg gctggttttt tattgttcac ggtttacgat
     2341 tttcattctt ccactcttct aaaatattta atcctaatcg tgtgaattct tgtttttctt
     2401 cttcgtcaag ttcatttatt ttacttaaca acccaagaat taaagccttg ttaatttcct
     2461 ccggtttttt acctaaaacc ttagccactt ctgcacccag gattattttt tgtcttgttt
     2521 cttcccttct gtcctgtctt gctttttttg ttcttaatgc ttgtagttta ttttgcaaat
     2581 ctgcgatctg ctgtgctgtc gttttactca tccattaccc cccaaaaata ttaaatgcct
     2641 tttgcattcc tctaacattt gaatattcaa aatgtttaat aatgcactct gctaaaatta
     2701 atttttcctt gtcactcaat tcatttattt ttttaaaaac aaggttctca tccgtgttaa
     2761 tgtctttaat gccttctgtt accttatcct cgacaagaga actaattgcc gagctaaggt
     2821 aatactctaa cctttcaaaa tctttatgcc cgttatcagc aaagaacttt ttgtttttta
     2881 ttatttgccc cgtaataaca tgcccatgct ctattaatat gctttcagat gcctctaata
     2941 tgtatttaaa aacactttcg atttttgcac cttctgcctc tgttataagt ttattcataa
     3001 taacctccta attatttatt ttgttattta ttacctccat tatttacttt agtcaaatat
     3061 attttagaat aaaacaaata atatttaata ttgggcgaag cctggggttc attacttatt
     3121 attttgaggt gcaaccgaaa aaataatatt agtaatgccc acttacacgt cgcttgcgac
     3181 gtatgggccc tgcggggcta aaaaccaaaa ttcaaaagac aaaaaaagta aggggtagac
     3241 tatggcgaca tttcatatca gctttaaaaa attacgaaga tcagaaggta aatcttcggt
     3301 ttatttatct gcatatcaaa accgagaaaa gacaaaagat aatcgaaccg gggcaacctg
     3361 ggattattca aaaaaagagg gatttttcgg ctctgctatc ctctcccctg ccggcacacc
     3421 tgccgaactg gtgaaggact caggcaccct ctggaatgcc gtagaggctg gcgagaagcg
     3481 aaaggatgcc gaattatgcc gttatctgga catagccatc cccaaggagc tggacgacgg
     3541 ccagaagaag cagatcgtcc tcgattactg ccaagaaaat ttcgtggatt atggaatgat
     3601 tgcagacata gcatttcatg atcttgatag tcataaccct catgctcatg taatgctaac
     3661 tttaagaact gttagcccaa atggatttgg gaacaaacaa atagaatgga ataatagaga
     3721 aaacattgaa gcctggcgag ttaactggga aataatagca aatgaccgat taaaaaaata
     3781 tggtcataaa tctcgtattg actcccgttc attaaaagac caaaaagaag aggcagaaag
     3841 aaaggctgtt tttgctaaaa ctccgaaaga gaaagccaag tggatttcaa aaactattga
     3901 actggacaga acgccaatga ccaggataca caggcaccaa tggcgaaaag gtcaaaaatt
     3961 aagagctatg gagcaaaaag aaaaagcatt attattaa
//
