LOCUS       NZ_CP070412            10405 bp    DNA     circular CON 04-DEC-2023
DEFINITION  Klebsiella pneumoniae strain B960 plasmid p2B960, complete sequence.
ACCESSION   NZ_CP070412
VERSION     NZ_CP070412.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN17911762
            Assembly: GCF_016925215.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Multidrug resistant Klebsiella pneumoniae_B960_hybrid assembly
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Veeraraghavan,B., Varghese,G.M. and Vijayakumar,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.8; Unicycler hybrid v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 123.0x
            Sequencing Technology             :: Illumina; Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/04/2023 04:38:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,411
            CDSs (total)                      :: 5,282
            Genes (coding)                    :: 5,141
            CDSs (with protein)               :: 5,141
            Genes (RNA)                       :: 129
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 61 of 141
            Pseudo Genes (incomplete)         :: 80 of 141
            Pseudo Genes (internal stop)      :: 23 of 141
            Pseudo Genes (multiple problems)  :: 19 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070412.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="B960"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2B960"
                     /geo_loc_name="India: Vellore"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2021"
                     /collected_by="Christian Medical College"
     gene            complement(join(<10405..<10405,1..164))
                     /locus_tag="JT722_RS26810"
                     /old_locus_tag="JT722_26810"
                     /pseudo=""
     CDS             complement(join(<10405..<10405,1..164))
                     /locus_tag="JT722_RS26810"
                     /old_locus_tag="JT722_26810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(353..1345)
                     /locus_tag="JT722_RS26815"
                     /old_locus_tag="JT722_26815"
     CDS             complement(353..1345)
                     /locus_tag="JT722_RS26815"
                     /old_locus_tag="JT722_26815"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.3"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_087874902.1"
                     /translation="MNKSIESLQVIRAIAACSVVLTHIYGASVPVIGSSGIFGVDLFFV
                     LSGYLMTITYRKNRTGYQFIKSRILRIYPPYLVLSLPLIIFSYFYKNISASDIFHNITL
                     LPTIHGNYDRINFVCWTLTYELYFYIVFSLCMYIFKGARNVILSTSFTMLVLFFLAGGV
                     NGGLAGWKDNSIHHMIGNYLILDFILGSLIGLAYNGYTYKKNAFPILLSIAIIITSCTI
                     IGHIHLPEPYNNYQLITFLTSGIPACVVIYITSKVSVDKIPLKKALIYIGTASYSIYLT
                     HLYAVFAFSKLTHNNAPEKFLCFVTSIVIGCVAYYTIERKIIIKKKNHV"
                     /name="acyltransferase"
     gene            complement(1432..2129)
                     /locus_tag="JT722_RS26820"
                     /old_locus_tag="JT722_26820"
     CDS             complement(join(1432..1880,1880..2129))
                     /locus_tag="JT722_RS26820"
                     /old_locus_tag="JT722_26820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1X4 family transposase"
                     /protein_id="WP_223335786.1"
                     /translation="MASISISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVNSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNLNLRQYLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1-like element IS1X4 family transposase"
     gene            2413..2523
                     /locus_tag="JT722_RS26825"
                     /old_locus_tag="JT722_26825"
     CDS             2413..2523
                     /locus_tag="JT722_RS26825"
                     /old_locus_tag="JT722_26825"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_139102090.1"
                     /translation="MDWISLAIAIALLVVAVYSGISYFREKKTISRVFRR"
                     /name="small membrane protein"
     gene            3577..3798
                     /locus_tag="JT722_RS26830"
                     /old_locus_tag="JT722_26830"
     CDS             3577..3798
                     /locus_tag="JT722_RS26830"
                     /old_locus_tag="JT722_26830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            3791..4678
                     /locus_tag="JT722_RS26835"
                     /old_locus_tag="JT722_26835"
     CDS             3791..4678
                     /locus_tag="JT722_RS26835"
                     /old_locus_tag="JT722_26835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_071579754.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKF
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPHQERAPDP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            4675..5373
                     /locus_tag="JT722_RS26840"
                     /old_locus_tag="JT722_26840"
     CDS             4675..5373
                     /locus_tag="JT722_RS26840"
                     /old_locus_tag="JT722_26840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579755.1"
                     /translation="MTRTFILLCTGVFAIAYALYELKHMGVYYPDASNPQLFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQSETDRYKQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYSGAEDVLREAFAQKESLLLQAVQNARKEAKQELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTVLARLEAEKAEERRRNACATAERRRRKLEKLQ
                     TTDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(5380..5778)
                     /locus_tag="JT722_RS26845"
                     /old_locus_tag="JT722_26845"
     CDS             complement(5380..5778)
                     /locus_tag="JT722_RS26845"
                     /old_locus_tag="JT722_26845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_071579756.1"
                     /translation="MANVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRFFGAFAPVSVEQEEEQVSGQRDTVTSDHNEQAIYILKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREEKRQNTNRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5778..5969)
                     /locus_tag="JT722_RS26850"
                     /old_locus_tag="JT722_26850"
     CDS             complement(5778..5969)
                     /locus_tag="JT722_RS26850"
                     /old_locus_tag="JT722_26850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_071579757.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAGADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6863..>7824)
                     /locus_tag="JT722_RS26855"
                     /old_locus_tag="JT722_26855"
                     /pseudo=""
     CDS             complement(6863..>7824)
                     /locus_tag="JT722_RS26855"
                     /old_locus_tag="JT722_26855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611476.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            8018..9559
                     /gene="istA"
                     /locus_tag="JT722_RS26860"
                     /old_locus_tag="JT722_26860"
     CDS             8018..9559
                     /gene="istA"
                     /locus_tag="JT722_RS26860"
                     /old_locus_tag="JT722_26860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="WP_002431311.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYANR
                     AAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLWEE
                     YCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPETGEI
                     RTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSATSRA
                     CKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHEIFYSL
                     ASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKKVRVHAD
                     YHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYSTRNEHMPE
                     AHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNLHKKYSKAR
                     LNAACARALETKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRGSEYYH"
                     /name="istA"
     gene            9574..10320
                     /gene="istB"
                     /locus_tag="JT722_RS26865"
                     /old_locus_tag="JT722_26865"
     CDS             9574..10320
                     /gene="istB"
                     /locus_tag="JT722_RS26865"
                     /old_locus_tag="JT722_26865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="WP_001016257.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTAE
                     ELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNILLT
                     GATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNSDLL
                     LLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAILDRL
                     VHGSIKIELKGESMRKIQSPLTEGDQ"
                     /name="istB"
     Typing          6018..6494
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.852"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 aggcgcttaa cctcgggcac ctccataccg ccatacttct tacgccaggt gtaaaagatg
       61 gcgtcggaaa tggcgtgctt acggcagagc tcacgggcag aaaccccggc ttcggcctcg
      121 cggagaatac tgatgatctg ttcgtcggaa aaacgcttct tcatgaggat gtcctcatgt
      181 ggcttatgaa gacattacta acatcacggt gtattaacca acggggagca ggtcactccg
      241 tgtatgcccc ggttttttcg cgctgtcgct gtctaaactg gctgtaaaaa tttacgccta
      301 ctgggatggg ctcgcagtag gttcattaac caattattaa atgctcatgt tatcaaacat
      361 ggttcttttt ttttattatt atcttccttt ctattgtgta atatgcaacg cagcctatta
      421 ctatcgaagt aacaaaacat agaaactttt caggtgcgtt gttatgagtt agtttagaaa
      481 atgcaaacac agcgtaaagg tgtgttagat aaatggagta tgaagctgtt cctatataaa
      541 tcaatgcttt ctttagaggt attttatcta cacttacctt tgatgttatg tatatgacta
      601 cgcatgctgg aatcccagat gttagaaatg taatgagttg gtagttatta tatggctcag
      661 gtaagtgaat gtggcctata atggtgcaac tcgtgattat tattgctatt gatagcaata
      721 tagggaatgc attttttttg tatgtgtacc cattgtacgc caatccaatt aaggagccaa
      781 gtatgaagtc taatattaga tagttaccaa tcatgtgatg aatagaatta tctttccagc
      841 ccgctaatcc gccgttaact ccacccgcta aaaaaaataa aacaagcatg gtgaatgaag
      901 tggataagat aacattcctt gcccctttga atatgtacat gcacaatgaa aatactatgt
      961 aaaaatataa ttcatacgtt aatgtccagc aaacaaagtt tatcctatca tagttaccat
     1021 gaatagttgg gaggagggtt atgttatgaa aaatatcaga tgcacttatg tttttataga
     1081 aatatgaaaa aataataaga ggcagtgata acacaaggta aggtggatat attcttaata
     1141 ttctgctttt tataaattga taccctgttc tgtttttgcg gtatgtaata gtcattaaat
     1201 aaccgcttag cacaaaaaac agatcaacac caaagatacc cgaagagcca attacaggta
     1261 cgcttgcgcc ataaatgtgt gtaagaacta ctgaacaggc tgctatggct cgaataactt
     1321 gtagtgactc gatactttta ttcatgaatt tacatactga aatgggttat tgaggtattt
     1381 tactggaaaa aactggatgt atcactaatt acttgtggtg atgacttcaa cttactgata
     1441 gtgttttatg ttcagataat gcccgatgac tttgtcatgc agctccaccg attttgagaa
     1501 cgacagtgac ttcctgccca gccttgccag atactgcctc agattcaggt tatgtcgctc
     1561 aatgcgctgc gtatatcgct tgctgataac gtgcagttct cccttcaggc gtgattcata
     1621 cagcggccag ccatccgtca tccataccac gacctcaaag gccgacagca ggctcagaag
     1681 acgctccagt gtggccagag tgcgttcacc gaacacgtgc gccacaaccg tcctccgtat
     1741 cctgtcatac gcgtaaaaca accagcgctg gcgtgattta gcgccgacgt aaccccactg
     1801 ttcgtccatt tccgcgcaaa caatgacgtc actgcccggt tgtatgcgtg agtttaccga
     1861 ctgcggcctg agttttttaa gtgtcgtaaa atcgtgttga ggccaacgcc cataatgcgt
     1921 gcactggcgc gacatccgac gccattcatg gccatatcaa taattttctg gtgtgtaccg
     1981 ggctgagagg cggtgtaagt gaactgtagc tgccatgttt tacggcagtg agagcagaga
     2041 tagcgctgat gtccggcagt acttttaccg ttacgcacca cgccttcagt agctgagcag
     2101 gaaggacaac tgatggagat ggaagccacg ggagcacctc aaaaacacca tcgtacacta
     2161 aatcagtaag ttggcaccat taccgtgatt tgttatatac ccaccacggg ggcattagtt
     2221 tttttatttt tttgatgata tgtacaaaat cagtggttag gaataatctt agcagcctta
     2281 acaaacacat acaaaaacag cggttatgtt tcatctcacc acaataagta acgacagtat
     2341 catgctaaaa aaagatgaca ttatctttat gaaatcataa caggctgttt ttattaaatt
     2401 aatcaggcat ttatggactg gatttcactt gctatcgcca ttgctctact tgttgtagct
     2461 gtctactcag gaatctcata cttcagagaa aaaaaaacaa tcagccgtgt atttaggcga
     2521 tagcgcccta tttaccataa tcccttctta cgcgcaccgc gaaaacggcc tcagggcgtt
     2581 tctggtggtt aacgggcaaa aggtggtggc aagtacaccg aaaaccgcca gaaacgcgcc
     2641 tgatttattt tacgcatatg catggctatg cacaccaccg catagccggt gatttcggcc
     2701 gagaaaaggg ttctggacgt taaaagcata aaagaacggg ccatccaggc ccgtcaaatg
     2761 aggacaactc tgtaaaagga ccgtcgcacc tggtgcggtg ccggcgacaa gaaaaagata
     2821 gctcatatca gcccctcacc ggagaaaaat atttcctggc cacctcaacc gccacacgct
     2881 gtgttcccgt taactcgcgc agataacgca ggaacatcct gttttcctct gcatgcgcct
     2941 cctcactgcc ctgtgcgtgg tattccacta tcgcgttcac cacggactca ccgacctgca
     3001 tcgtctgacc tcatctgcac tttcatcaca ccgtttcaga tcccgcctcc tcgccatgat
     3061 gtgaaacggt gaccgggcag gcaaattttt tgtctgtccg gtcaccgttg gcggggtccg
     3121 gggcggtcag tcccggtgtt ttttattccg ccaaatcagc acctgcgaca cccgctatgt
     3181 acgacgcata aaatgcgcct gtacatagcg ggtgtcgcta ttaaactgag tcaggcgcaa
     3241 catttaaaaa acaacattca cacgcacgaa aaacctggct agtatttagt caaaccatga
     3301 ggaggcgcac tctgacgcgc acctgcctac cggcagcagg taaaacagcc gggtcccccc
     3361 ggggatactg taccggacag cccaatccga gaatcctgag aaaacagcca aaatctgcga
     3421 gaccggcaat caggtcaggc cattccctgg cttaagggac gtttttacag ccgcgagcag
     3481 tccgggctta accacgttac ggtttaaacg agggcgatcg gcgcaggccg cagtggattt
     3541 ccactggcgt tcttacaact caacaccgga gttcctgtgg aatttgatac cgccgccgaa
     3601 ctggccgccc tgcaggcgca gacccgccgt atccgccagg tccgctaccg tccctcccgc
     3661 cttgaccgct ataccggcga actgctgagc ctgtaccagg ccggggccag tgcagcggag
     3721 ctgcaacgct ggctccgggc ccggagaatc aaagtcgtgt tgtccacggt tacccgctgg
     3781 ctggaaaaaa atggctaaat tcgccagcgc tgcccgccag gcggctggcg tgataaaacc
     3841 actccaggga aatattctgc gctctgtcgg tacggtccgg aattacgagc agggactgac
     3901 ccgcgtcgca gagtggatga aatttaaccg catcaaccac ggtgatttac gcacactgac
     3961 accggcgcag gccatcacct acctggagca gcgcggggag caggtcggcc agaaaacgct
     4021 cgatatggaa cgccaggcca tccaggccat gatgatccac gtgacgcaga ccctccctgc
     4081 cggcgaacac ctgccggtgg tgaaatcgga gcaggcacag atcctgagca gccgggccta
     4141 cagcccggac caggtggccc tgattgccgc agcacaacag cccgccaacg ccctggccac
     4201 cgcgatcgcc ctggccgccg gattacgcgc ccatgaactg ctgaccctcg cgccccacca
     4261 ggagcgtgca ccggatcccc gtccggcagc ggacagtaaa tttgccggtc gggagggtgt
     4321 gatttacacc gttcagggca aaggcggcct ggtcagggaa gttctgctgc cgtcacgcct
     4381 ggctgaacgt ctcgagcatg tcaggctggc cagcccggtc cgggtgaccg atcgcggcgt
     4441 gttttaccag agtcagtacg ccattggcgg aggccagcgc tggtcaaacg ccttcagtgc
     4501 cgcctccatg cgtaccctgg gctggtcacg gggggcacac ggcgtgcgtc acagctacgc
     4561 ccagcaacgg atgcaggagc tgcaaaaact cggtctggtt cgtgatatgg ccctgcgtac
     4621 cgtgagccag gaaatgggcc attttcgtcc ggaaatcacg gagacatacc tgcgatgaca
     4681 agaacgttta ttttattgtg taccggtgtt ttcgccatcg cgtacgccct gtatgagctg
     4741 aaacatatgg gcgtttacta tccggatgcc agcaatccgc agctgttcac tggctggaag
     4801 gcggtcaccg aactcggtcc cgcaatcctg gctacgggga tcgcagccac agcgttctcg
     4861 ctgtggctgt tcattttgtt tttcagaggc tcagagtccc gccggataca gagcgagaca
     4921 gacaggtata agcaggctct gagggaggca gaagaggctc acagagccag acaaaatacc
     4981 cttattcaga atcacaacct ggagacagcg gccctgcgaa gccactacag tggtgctgag
     5041 gatgtactgc gggaagcgtt tgcacaaaaa gaaagcctct tgcttcaggc ggtgcagaat
     5101 gcccggaaag aagccaagca ggagctggaa gaacagaggg aggcactgaa tcagcgcgaa
     5161 tccatcctgc agaaaaagga gctggcgctg gaaacaaccc tggagaaggc ccggaaatat
     5221 gtggccgggg agcggcataa caccgtgctg gcccgtctgg aagcagagaa agcagaggaa
     5281 agacggagaa atgcctgcgc gacggcggaa cggcgcagac ggaaactgga aaaactccag
     5341 accactgacg gaacagcagg gcaaaaacac tgaggcattt caggactgcc agaaacgcca
     5401 ccaggggcgg ttcgtgttct gccgcttctc ttcccggttc tccagcaaca acatcgcctg
     5461 tttcaggctg tcgatatgtg aatcttttga ggcaagaagg gtgttcaggt gttcaatttc
     5521 ctgtttcaga atgtaaatgg cctgttcatt atggtcagaa gtgactgtgt cacgctgtcc
     5581 actgacctgt tcttcctcct gttcaacgga tacgggcgca aaggcaccaa aaaaccgcaa
     5641 aagctctgaa gtatcgagca ttttcgcatt ccgttcaccc cgacaggtgg acagttcacc
     5701 ggctttaatt aacctgtgca gggttgtcct gctcttaccc gtcaggcgtg ctgcctcact
     5761 gatcgacacg ttcgccatca gtcaccctcc tgtagtgcat ccagtcggag ggacagtgta
     5821 cggaaaagat gctccgcatc atcgtggagt ttctcacaaa ggtcagcccc cgcatcgaga
     5881 tcaagctcgt taagcttctc cagcaggagc agggactgat tctggatgaa tttagccatg
     5941 ttcagggcag ttttctgttg tttcgtcata aggggcctca gggcgtcagt gctcagtgta
     6001 aaaccggtct cttttccgct tcctcgctca ctcagtcgct acgctcctgc cgttcggctg
     6061 cggcgagcgg acgggacaca cagaagagtc atcgctatca ccaggaggag aagagcctgt
     6121 ggaaaaggcc acggcgttgc cgtttttcca taggctccgc ccccctgaca agcatcacga
     6181 aatctgacgc tcaaatcagc ggtggcgaaa cccgacagga ctttaaagat accaggcgtt
     6241 tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta ccggtgtcat
     6301 tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcagttcc gggaaggcag
     6361 ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgactgc tgcgccttat
     6421 ccggtaacta tcgtcttgag tccaacccgg taagacatgc aaaagcgcca ctggcagcag
     6481 ccactggtaa ctgatttaga ggagatagtc ttgaagttat gcgccggtta aggctaaact
     6541 aaacgaacag ttttggtgac tgtgctcctc caagccagtt acctcggttc aaagagttgg
     6601 tagctcagag aaccttcgaa aaaaccgccc tgcaaggcgg ttttttcgtt tactgagcaa
     6661 gagattacga tgctaaagaa aggatctcaa gaggatcctg ttccatatgc ctacccctga
     6721 agtgaaacca gcaccactcc gcgacaccaa ccatcaaaat aaaactattt aactcgttgt
     6781 ttttgataag aaaaaaacat atataatcat gatccataat catccaacgc ggttgacctg
     6841 cccccaggat tagatacaac cttcagttag taatgtcggt tcgtttttct tcatatttcc
     6901 cgtttcgcca gcccgccgca aattcagccg gagtctggta gttcagcgat gaatgtggac
     6961 gacactcgct ctaatcctgc ggccagtcat taatcgtttt cctcgcgtga acgatatcgc
     7021 ggaaccagtg ctcattgagg cactcatccc tgaatcgtcc gttaaaactt tcaataaatc
     7081 cgttctgtgt tggcctgccc ggctggataa gccgcagctc caccccatgc tcataagccc
     7141 actggtcgag tgctctgcag gtaaactccg ccccctggtc agttcttatc gtcgccggat
     7201 agccgcgaaa cagtgcaatg ctgtccagca ttcgcgtgac ctgaacgcct gaaatcccaa
     7261 aggcggcggt aattgtcaga cactccttcg tgaagtcgtc cacgcaggtc aggcacttga
     7321 tcctgcgacc ggtggccagc gcgtccatga caaaatccat cggccaggtc aggttgggcg
     7381 cctccgggcg gagcagcggc agacgttctg ttgccagacc tttacgacgt cgtctgcgtt
     7441 ttacacccag cccgttaaga tggtaaagac ggtacacgcg cttgtgatta acatgaaggc
     7501 cttcacggcg cagtaactgc cagatgcgtc ggtagccaaa acgcctgcgc tccagtgcca
     7561 gctcagtgat gtgccctgat aaatgcgcat cagccgcggg acgctgagcc tcatagcggc
     7621 aggtcgacag ggacaaacct gtaagcctgc aggcacgacg ttgcgacaga ccggtcgcat
     7681 cacacataaa ctcaacggct tcccgtttct ggtctgtcgt cagtactttc gccccagagc
     7741 cacctgaagt gcctccttat ccagcatggc ttcggcaagc agtttcttga gtctggcatt
     7801 ctcttcctca agcgacttaa ggcgtgcgta ttttcatgaa aggagatcac tcaataactt
     7861 ccatcgagat cgggtaataa catttgaaca gatcgctgaa taacatcgat ggagatcact
     7921 tttgactcat tttgttattc agtgatctcc atcaatgtta ttggaacttc acaggtgtgt
     7981 tgatctgtat cttttgccat tccggtaaag gatacctatg ccaacagttc caatttctat
     8041 gagaaaactt aaagaaattc ttaggcttaa atacggtgtt ggactcagcc atcgacaaat
     8101 tggtcgtagt cttgcaatct ccccttccgt tgtatccaga tatgctaatc gggcggctca
     8161 acttggcata aagcagtggc ccttacctac aggatgggat gatacaaaac taaaacatgc
     8221 gttccttcag acccaggtta agatgaagaa gcactctctg cctgactggg ctacagtaca
     8281 ccgggaactg cgtaataaat gcgtgacgct gcagctactc tgggaagaat actgtgagcg
     8341 taatccaggc ggtttttaca gctataacca ttactgccgg atgtaccgtg aatggctcaa
     8401 aaccacttca ccatcaatgc gtcaggtaca taaagctggc gaaaaacttt tcgttgatta
     8461 ctgtggacct accgttggcg ttaccgaccc tgagaccgga gaaataagaa ctgctcaggt
     8521 catcgtagct gttctcgggg catcaagtta cacatgggca gaggccacct ggtctcagca
     8581 gcttgaagac tgggtgatga gtcatgttcg ctgcttccag tggttgggtg gcgttcctga
     8641 acttgttgtt ccggacaatc tgaaaagcgc cacatccagg gcatgtaagt atgatcctga
     8701 cgttaaccct acctaccagc agatgcttga gcattataat gtcgcagttt tgcctgcgcg
     8761 gccacgtaaa ccgaaagata aagccaaagc tgaagttggc gttcaggttg ttgaacgctg
     8821 gatcatggcc cgaatcaggc atgagatctt ctacagcctt gcatcgctta atcagcgcat
     8881 tcgggagttg ctggaaagac tgaataacaa aataatgcag aagttgggtt attcacgtgc
     8941 agaactcttc atccagcttg ataaacccgc actgaagcct cttcctgaag ccagttacag
     9001 ttacaccctg gtgaagaaag tcagagttca tgccgattac cacgtggaaa tcgacaaaca
     9061 ttactactcg gttccatgtt cgctgttagg ccagcaactg gaagcatgga tctccggaga
     9121 actggtaaga ctcttcaatc aggggcagga ggttgctgtg cacccgcgca agcgtactta
     9181 tggctacagt acccgcaacg agcacatgcc tgaagctcat cgacagcatg ccacctggac
     9241 gccagagcgt cttctggaat gggcggggca cataggcagt gaaactcata gttatgtgct
     9301 tcatatactg aactctcgtc cacatccgga acaaagctat cgcttctgcc ttggactcct
     9361 gaaccttcat aaaaaataca gtaaagccag acttaatgca gcatgtgcaa gagctctgga
     9421 aacaaaggta tggcgtctgt caggtattaa atcgatcctg gaaaaaggtc tggataaaca
     9481 acctgttcag gatccaaaac cagatctgtt atccacgatg gaacacgaaa acgtacgcgg
     9541 cagtgagtat taccactgat acgggatcca atgatgaatc atctttacga acaactgacc
     9601 gcacttaaac tcaccggctt ccgtgatgcg cttaaaaagc aacttgctca accgggcaca
     9661 taccaggagc tgggcttcga agaacgcctg tcattactga cagcagaaga actaacctgc
     9721 cgtgaaaaca ggaaggcaga gcgtctgatc aaacatgcac ggttcagact taatgctgag
     9781 ttatcaaagc tggattatcg taacaataga gggctggaca gggccctcat ccgttcactc
     9841 agtcagggaa actggttaac cctgaaacaa aatattttac tgaccggggc caccggcagc
     9901 ggtaaaacgt tcctggcatg tgcacttggt cataatgcct gccgacaggg atacaaggtc
     9961 tactattatc gccttaaagc gctgatggaa cagtgctatc aggggcatgc tgatggaaga
    10021 tacagcaaac ttttgaccag gctgaataat agcgatctgc tgcttctgga tgactggggg
    10081 ctggaacctc tctcatcaga acagcgtagc gacctgctgg aaatagtgga tctgatgtac
    10141 caacgaggct caatcatcgt agtgagccag ttgccggtgg aaaactggta caaaatgatc
    10201 ggagactcca cacatgcgga tgccatccta gatcgactgg ttcatggcag tatcaagatc
    10261 gaacttaaag gagaatcaat gcggaaaata caatctccgt tgaccgaagg agatcagtga
    10321 aggtaattta aaaacggttc tgtgaaagtg acacgaaccg atctccatcg atgttactca
    10381 ccgatctcct tcacggtaat acgca
//
