LOCUS       NZ_CP070190             1908 bp    DNA     linear   CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1330 plasmid unnamed12.
ACCESSION   NZ_CP070190
VERSION     NZ_CP070190.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357472
            Assembly: GCF_016903515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 635.94x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:11:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070190.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1330"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1330"
                     /db_xref="taxon:573"
                     /plasmid="unnamed12"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..180
                     /locus_tag="I6K60_RS26745"
                     /old_locus_tag="I6K60_26745"
     CDS             <1..180
                     /locus_tag="I6K60_RS26745"
                     /old_locus_tag="I6K60_26745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204724675.1"
                     /translation="GNLRETRRNAKGNLCTGQDVFLEYRHHDAGTKNDPSGLRPDTLPT
                     PHAARLKSASVRRS"
                     /name="hypothetical protein"
     gene            641..1630
                     /locus_tag="I6K60_RS26750"
                     /old_locus_tag="I6K60_26750"
     CDS             641..1630
                     /locus_tag="I6K60_RS26750"
                     /old_locus_tag="I6K60_26750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001529700.1"
                     /translation="MKNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MALCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGEILNRMNAAFQRLKDRKEFRPVQGWIRTTEVTRSS
                     DGSAHPHFHTLMMVPPSMLSGDGYVKHARWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(1357..1548)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
ORIGIN
        1 ggaaatctca gggaaacccg aaggaatgcc aaaggaaatc tgtgtaccgg acaagatgtg
       61 tttttggagt accgccatca cgatgcgggt accaaaaacg atccgtcagg gctgcgcccc
      121 gacaccctgc caacacctca cgccgccaga ttgaaatctg ccagcgttcg ccgttcctga
      181 aatcgtcaaa cgctaaatcg tggggtcccg ctggcttgcc agtgggttcc gatttagtga
      241 ttgacgaccg cgcccgtaaa acactgccga caggtggttg tgatcacgca gtgagcgcaa
      301 ccacctggta agcggttttg ggtttttttg ggggtgattt aacgcggttt tcgggtggca
      361 aatctcgcgc gcgatccttg tatttatact taagggataa ctctgggatt caaattggcc
      421 tccagccctt gtcccgcaag gcttgagggc acatttgctc cgaaatgggt ttagaagtat
      481 tctgtcggaa gtggtcgcgt taaaagcgac ccccgcaacg acgggcatcg ttcgggggcg
      541 tgaccacctg tagagattcg aattggtgat acggctaaca caggtgatat ggcaatgata
      601 acacgtcaga tgagccatcg cccacaatcg ggtggtgtgc atgaaaaatc ctgaactcca
      661 gaacctcacg gactattccc cctcagatgc gccctgggac gctcacagaa gcgtttctga
      721 cgacgtggga gggatttacc tgcttgcggc cgaatatgag cgctacgggg cgcgtatggc
      781 cctctgtggc ggtttattgc gtttcgggtg gtctaccctc aaggaaaccg gagaaacgcg
      841 tctgaggctg cgtgaggcgc atttctgccg tgtccggcac tgtcctgtct gccagtggag
      901 gcgctccctg atgtggcaag cccgtttcta ccagtccctg ccgcgtatcg tggcggatta
      961 ccctgatgcc cgctggatgt tcctgaccct gaccgtgcgc aactgtgcga tcggggagct
     1021 gggcgagata ctgaaccgga tgaatgcagc tttccagcga ctgaaggatc gcaaggagtt
     1081 ccggcctgtg cagggctgga tacgtaccac ggaagtcacc cgcagcagtg acggcagcgc
     1141 acatccccac ttccacaccc tgatgatggt gccgccctca atgctatctg gtgacggtta
     1201 cgtaaaacat gctcgctggg ttgaactctg gcgggagtgc ctgcgggtca gttacgaccc
     1261 gaatgttgat gtccgggcag taaaaccccg gaagccgaag gacggcgaat cgctggcctg
     1321 cgcgaccgcc gaactggtgc gcggtgcggt ggccgaaacc ctgaagtatt ccacgaagcc
     1381 ggctgacatg gtggccgatc ctgaatggtt cctcgaactg acccgccaga cgcacaagcg
     1441 ccgctttgtg gcgactggcg gcgcactcaa agacgtactg aagctcgatc aggaaaccga
     1501 cgcagacatg gtgatcgggg atgatatttc cgagggcgac gatgacggtt cccgcatcgc
     1561 gttcgagtgg aaaactgagg tcagaaagta ccgtcgctca ccgacgaaag ataaaaccga
     1621 atcagagtaa cggggtgtcg gggctgcgcc ctgaccatga ggactttggg catccgtcac
     1681 cttggtggcg gtgtaccaaa gtcacatctt gtccgattgg ttttactccg atgcaggata
     1741 gggctaacgc cgcaaagtgc tagcactcga ttgcctagtt cggaatccga acttagggtt
     1801 ttgcgatccg taatacggat tttttttgac gggacaagat gtgtttttgg agtaccgcca
     1861 tcacgatgcg ggtaccaaaa acgatccgtc agggctgcgc cccgacac
//
