LOCUS       NZ_CP070189             2422 bp    DNA     linear   CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1330 plasmid unnamed11.
ACCESSION   NZ_CP070189
VERSION     NZ_CP070189.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357472
            Assembly: GCF_016903515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 635.94x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:11:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070189.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1330"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1330"
                     /db_xref="taxon:573"
                     /plasmid="unnamed11"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(126..1121)
                     /locus_tag="I6K60_RS26735"
                     /old_locus_tag="I6K60_26735"
     CDS             complement(126..1121)
                     /locus_tag="I6K60_RS26735"
                     /old_locus_tag="I6K60_26735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020240588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_204724673.1"
                     /translation="MQNPKLRNLTDYSPDDKPWDAHKSQSDDVGGIYLRAAEFESYAAR
                     MRDCGGLLRFGWSTLKDTGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPKIV
                     ADYPDGRWMFLTLTVRNCDVAELGETLTAMNAAFQRMKDRKEFKSVQGWIRTTEVTRGR
                     DGSAHPHFHTLMMVPPSMLSGRDYVKQYRWVELWRDCLRVDYDPNVDVRAVKPRKPKDG
                     QTLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LEQETDQDMVIGDDISDGEDDGSRLAFGWDSTPRKYVRLPERDKFKSDSD"
                     /name="protein rep"
     gene            complement(1999..2184)
                     /locus_tag="I6K60_RS26740"
                     /old_locus_tag="I6K60_26740"
     CDS             complement(1999..2184)
                     /locus_tag="I6K60_RS26740"
                     /old_locus_tag="I6K60_26740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_204724674.1"
                     /translation="MTKQQRIDGFRKAEASLKLEGMDPSGTPFYESVKARIISGEITYE
                     QGKAEILSHHKARAEG"
                     /name="antitoxin VbhA family protein"
     Typing          216..406
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.375"
                     /coverage="98.0"
                     /evalue="1.4200000000000001e-44"
ORIGIN
        1 aatccgaact tagggttttg cgatccgtaa tacggatttt ttttgacggg acaagatgtg
       61 tttttggagt accgccacac gcagcggcat ccaaaaacaa catggtcagg gcaggccccg
      121 ataccctaat cagaatctga tttaaactta tcgcgctcag gcaaacggac gtattttctt
      181 ggcgtcgaat cccagccaaa ggcgaggcgt gaaccatcgt cttcaccatc ggaaatatca
      241 tccccgatca ccatgtcctg atccgtttcc tgctccagtt tcagaacgtc tttcagcgcg
      301 ccaccggtcg caacaaaacg gcgtttatgc gtctgccggg tcagctccag gaaccattcc
      361 gggtcagcaa ccatatcagc cggctttgtc gaatatttca gagtttcagc aacggcaccg
      421 cgcaccagtt cggcagttgc acatgccagc gtctggccat ctttaggctt acggggtttc
      481 actgcccgaa catcaacatt cggatcgtaa tcaacacgca ggcagtcgcg ccagagttca
      541 acccagcgat attgttttac gtaatcacga ccggaaagca tcgagggagg aaccatcatc
      601 agggtgtgaa agtgggggtg tgcagagcca tcacgaccgc gggtaacttc tgtggtgcga
      661 atccatccct gtacggattt aaactccttg cgatccttca tgcgctgaaa tgcggcattc
      721 atggcagtaa gcgtttcgcc gagttcagca acatcgcagt tgcgaacggt gagcgtcaga
      781 aacatccatc ggccatcagg gtaatcagcg acgattttcg gcagagactg ataaaaacgg
      841 gcttgccaca tcagggagcg cctccactgg cagacagggc agtgacgaac gcggcagaaa
      901 tgcgcctcac ggagccgtaa acgcgtttct ccggtgtctt taagggtaga ccagccaaaa
      961 cgcaggagac cgccacaatc gcgcatacgg gctgcatacg actcaaattc agcagcacgc
     1021 agatagatac cgccaacatc atcagattgt gacttatgag catcccacgg cttatcgtcg
     1081 gggctgtagt cagtgaggtt tcttagctta gggttttgca tagcacctgc tccaatatgg
     1141 gcaggcactg gaagggtgtg ttatcatttc cgtatcacct gttgagagtc gatttgaaac
     1201 cgtatttagc caggtgatcg cgcccctgaa gtgctggtaa cacttgcagg ggtttctcat
     1261 atcatcgtgt cagtaagtaa ggtcttggga accaccccaa gtaatcaaaa attagttcgc
     1321 ggacacgatc acctgtcaga aatcatactg ccaaaacctt ttccgagcaa attggctctc
     1381 aggccttgcg gcacaaggga tggaagcgaa ttttaatccc agagttatcc cttaagtata
     1441 aatacaagga tttcgcccct ttcagggtcg aaaaaccaac ccccaaaagc agcattcagg
     1501 cactcaaaca ctcaagcatt cgcacgctca aaaattaagc aaaccacgtt aaatcgcccc
     1561 ccctgagaaa tgtcagattg agacaaacgt gcagtattac attcactgca cgcggctgtt
     1621 tccgttgcaa atcgcaacaa gagtccacta ttaaacagag tggaccccga ttccgaacgg
     1681 cgtgtgcctc ctcgcagagc tgcgggggca atccccgttc ctcatcgcgg ccgcacgact
     1741 ttaccccgca taatcctgat ggcggattta aacgaataag gcataccgag caaaacgaaa
     1801 atacccccac ggcatcacct gtagggcagg gcttacgcac ctaccccaca ggcaacgcct
     1861 tgaagggggt aaaaagcggc tttacagggc gatggctcac tacgtgaacc atcacccatc
     1921 acaagggtat gaggggtgca gtcgtacaac cactaaatcg gaaccctaaa gggatccccc
     1981 gatttagaag ttgacgattc aaccttctgc gcgggctttg tggtgggaaa gaatctcagc
     2041 tttgccctgt tcgtaggtga tttcaccgga gatgattcgg gcttttaccg actcataaaa
     2101 gggagttcca gaaggatcca taccctccag ttttaacgat gcttccgctt tacggaatcc
     2161 gtcgatcctt tgctgttttg tcatgaggtt tcctttgagc agaatgcgaa taaggcaggc
     2221 gtaaagttgg ctaactttac atgtcctgcc cgaactgtgc cgttggttat gcgtgtatac
     2281 gtgtatgcac gcacagtgga aatctcaggg aaacccgaag gaatgccaaa ggaaatctgt
     2341 gtaccggaca agatgtgttt ttggagtacc gccacacgca gcggcatcca aaaacaacat
     2401 ggtcagggca ggccccgata cc
//
