LOCUS       NZ_CP070188             2531 bp    DNA     linear   CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1330 plasmid unnamed10.
ACCESSION   NZ_CP070188
VERSION     NZ_CP070188.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357472
            Assembly: GCF_016903515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 635.94x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:11:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070188.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1330"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1330"
                     /db_xref="taxon:573"
                     /plasmid="unnamed10"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            720..947
                     /locus_tag="I6K60_RS26720"
                     /old_locus_tag="I6K60_26720"
     CDS             720..947
                     /locus_tag="I6K60_RS26720"
                     /old_locus_tag="I6K60_26720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1313..1498
                     /locus_tag="I6K60_RS26725"
                     /old_locus_tag="I6K60_26725"
     CDS             1313..1498
                     /locus_tag="I6K60_RS26725"
                     /old_locus_tag="I6K60_26725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1789..1980)
                     /locus_tag="I6K60_RS26730"
                     /old_locus_tag="I6K60_26730"
     CDS             complement(1789..1980)
                     /locus_tag="I6K60_RS26730"
                     /old_locus_tag="I6K60_26730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021243041.1"
                     /translation="MTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQILSETEPAV
                     RTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1546..1633)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          3..485
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="83.96"
                     /coverage="95.0"
                     /evalue="2.29e-126"
     Typing          2260..2531
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.13"
                     /coverage="99.0"
                     /evalue="3.12e-90"
ORIGIN
        1 ttaaagatac caggcgtttc cccctggtgg ctccctcgtg ctcccccgca ttcctgcctt
       61 tcggtttacc ggtgtcactc cgctgttatg gccgcggttt ctcattccac gcctgacact
      121 cagttccggg aaggcagttc gctccaggat ggactgtatg cacgaacccc ccgttcagtc
      181 cgaccgctgc gccttatccg gtaactatca acttgagtcc aacccggaaa gacacgacta
      241 atcgccattg gcaacggcca ttggcaaaca ggatgtgcga gagatgtctc aagagttctt
      301 gaagtggcgg ccttagtgag gctacactga aaggacaaat ttggtgacat gtctcgcaca
      361 agactgttac cacggttagg ggatctgcca ggattaccta accttcgaaa aaccacctgc
      421 cagggtggtt ttttcgttta cagggcaaga gattacgcgc ggaaaaaaag gatctcaaga
      481 agatccttta taaattagac atccaacctc acaaagagga atacttaaat catcttggta
      541 tatgtatcgg ccggattaca tctcatataa gcctcctccc cgcctgacac cacactttga
      601 ccatagcacc tcgcgcctaa ctcgatatac agcccaccat caagcctctt ttcacataaa
      661 aaatgaccta tagttagatg gttagtgacc aaaactgata tggataaaag gagctggctt
      721 tgaaaaagat ttttgtttca gtgttcgcag cggcagttgc gctttcagcc ctcaccggct
      781 gcacgcgcac aagctatgcc atacacacca atgacggtcg cactattgtc agcgacggca
      841 aacctaaaga atcagactca ggcctgcttg gctatacaga cgcgaatggt gtcaaacagc
      901 agatcaataa aaccgacgtt aaagaagtct ccgaaattcc gcactgagca tcttctccga
      961 aaaaggccgc taatgtggcc tttttcttta cctgtggttc tccgccaaaa tcccagcaaa
     1021 ttgcatcacc aaagctaaaa gctttcaggg ttgtctaaaa agcacaagac ataagaggaa
     1081 gtgcggtatt ttataatcaa gcccccaaga atttaccata acatccgttg cccgcaccgc
     1141 ctgagacgcg ttcagcgcgt tcctgacgaa accatgacaa aaaccacaac aaaccacccc
     1201 ggaacccgtc agaaacgcgc ctgttaaatt ttaacggcat gcatgactat gcaccagaat
     1261 gacgccatgc tcttttcacg caaaaatcat caccagacgg ggaaaatcac cagtgaccag
     1321 acaggaatcc gccgccctca atatggccaa atttatccgc gcacagacac ttctcctcct
     1381 tgagcggctc gagcagatgg atctggatga ggctgccggc tgctgtgagc acctgcacga
     1441 tcaggccgaa gcgctttacg ccatgctgaa cgcacagata ggcgaggaaa atgcgtgaaa
     1501 atcggtgaac gggtgcgcaa ttcagtgcgc ggccgtgagg cgatggcggg gtgtcggggc
     1561 gcagccctga ccagggtatt tgtgatgccg gcgcgtgcgc ggtattacaa atgcacatcc
     1621 tgtcccggaa cggacaccgg gaaacagcaa aaaaaaccgg gcggcacgcc cggaactcaa
     1681 tcaagttaga ttagattact ctcactcgtc cataacagca tcatggaacg acgaccaccg
     1741 tccgtgacgg ccgcctcgtt taagtatgga cagaaataca gaaaatgctc aggacgaaat
     1801 gtaatgaatg cgaacggatt caagaaattc gagcatgaca gtccttacgg ccggttcggt
     1861 ttcagacaaa atctgccggt atgcatccag catcatggct ccggcatccc ctccggcacg
     1921 ccgtagccag accgaaacaa cggacacaag caggtgtcgc tcatcatcac taagagtcat
     1981 cagggctccg gaagaaaaac caaacatggc ttacctactg ttaacggcaa cgcagaagaa
     2041 aggtttacag ggtagcgggc agggatgaaa aaaaaaaaat tgcagtcagg ccggaacggc
     2101 tgctgaaccg gtacaggcgg gttccgggcg cagcgccgga ccagtcacgg agcactagcg
     2161 gagtgtatac tggcttaaga tgtagcatgg cagtaacaca tagcattaat gccacataag
     2221 acaggcgcag aaagtgaaag aaaacgcgtc aggagaaaat gtgatacaga atatattccg
     2281 cttcctcgct cactgactcg ctccgctcgg tcgttcggct gcggcgagcg ggacgggctt
     2341 acggatggat cagaaatttc ctgggggatg ccaggaagat acttaacgaa gaaagcataa
     2401 ggccgcgcca aagccgtttt tccataggct ccgcccccct gacaagcatc acagaatctg
     2461 acgctcaaat cagtggtggc gaaacccgat gcggcttaaa gataccaggc gtttccccct
     2521 ggtggctccc t
//
