LOCUS       NZ_CP070187            12439 bp    DNA     linear   CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1330 plasmid unnamed3.
ACCESSION   NZ_CP070187
VERSION     NZ_CP070187.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357472
            Assembly: GCF_016903515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12439)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12439)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 635.94x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:11:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070187.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12439
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1330"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1330"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <79..651
                     /locus_tag="I6K60_RS26645"
                     /old_locus_tag="I6K60_26645"
                     /pseudo=""
     CDS             <79..651
                     /locus_tag="I6K60_RS26645"
                     /old_locus_tag="I6K60_26645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /name="SDR family NAD(P)-dependent oxidoreductase"
     gene            689..934
                     /locus_tag="I6K60_RS26650"
                     /old_locus_tag="I6K60_26650"
     CDS             689..934
                     /locus_tag="I6K60_RS26650"
                     /old_locus_tag="I6K60_26650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="WP_001354008.1"
                     /translation="MKIEIMEYNPDWTKNFEEEKIKLLHFFGSHAVAIEHIGSTAIPNQ
                     RAKPVIDIFIGVSPFAELPFISAFLMQRSITTLRQI"
                     /name="GrpB family protein"
     gene            1403..2194
                     /gene="sul3"
                     /locus_tag="I6K60_RS26655"
                     /old_locus_tag="I6K60_26655"
     ARG             1404..2191
                     /name="sul3"
                     /gene="sul3"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul3"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2197..2472)
                     /locus_tag="I6K60_RS26660"
                     /old_locus_tag="I6K60_26660"
     CDS             complement(2197..2472)
                     /locus_tag="I6K60_RS26660"
                     /old_locus_tag="I6K60_26660"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_109023896.1"
                     /translation="MNIGLFGFTNKHYYLATKTQEKYTKPFGSDGSLSPLDYSVGIGTG
                     LELGHFQFMFGYDYPLSNSSKDPDLSQVRQHNFRASIAYSFRKLKK"
                     /name="hypothetical protein"
     gene            2495..3127
                     /locus_tag="I6K60_RS26665"
                     /old_locus_tag="I6K60_26665"
     CDS             2495..3127
                     /locus_tag="I6K60_RS26665"
                     /old_locus_tag="I6K60_26665"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="WP_020802982.1"
                     /translation="MKVPQVRGMEFYPSCIEKGMRSERALKLAIAEMYVKGVSTRRVSD
                     IVEILCGTEVSSSQVSRLAKELDEEITSWKAQPVGQIQYLVLDATYESVRVGSHVVKQA
                     LLVAIGVDYSGNRHILDAEVANSEAEVNWRSFLEGLVRRGMHGLRMITSDDHSGLRAAI
                     DAVFPGILWQRCQFHLQQNAHSYVTKKDEIPLIAADIRKVFNRNMSR"
                     /name="IS256 family transposase"
     gene            complement(3374..3706)
                     /gene="qac"
                     /locus_tag="I6K60_RS26670"
                     /old_locus_tag="I6K60_26670"
     CDS             complement(3374..3706)
                     /gene="qac"
                     /locus_tag="I6K60_RS26670"
                     /old_locus_tag="I6K60_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /protein_id="WP_000800531.1"
                     /translation="MKNWLFLAIAIFGEVVATSALKSSHGFTKLVPSVVVVAGYGLAFY
                     FLSLALKSIPVGIAYAVWAGLGIVLVAAIAWIFHGQKLDLWAFVGMGLIVSGVAVLNLL
                     SKVSAH"
                     /name="qac"
     gene            complement(3829..4667)
                     /gene="aadA1"
                     /locus_tag="I6K60_RS26675"
                     /old_locus_tag="I6K60_26675"
                     /pseudo=""
     ARG             complement(3880..4667)
                     /name="aadA1"
                     /gene="aadA1"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(4760..6019)
                     /locus_tag="I6K60_RS26680"
                     /old_locus_tag="I6K60_26680"
     CDS             complement(4760..6019)
                     /locus_tag="I6K60_RS26680"
                     /old_locus_tag="I6K60_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001256773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol efflux MFS transporter CmlA1"
                     /protein_id="WP_000095725.1"
                     /translation="MSSKNFSWRYSLAATVLLLSPFDLLASLGMDMYLPAVPFMPNALG
                     TTASTIQLTLTTYLVMIGAGQLLFGPLSDRLGRRPVLLGGGLAYVVASMGLALTSSAEV
                     FLGLRILQACGASACLVSTFATVRDIYAGREESNVIYGILGSMLAMVPAVGPLLGALVD
                     MWLGWRAIFAFLGLGMIAASAAAWRFWPETRVQRVAGLQWSQLLLPVKCLNFWLYTLCY
                     AAGMGSFFVFFSIAPGLMMGRQGVSQLGFSLLFATVAIAMVFTARFMGRVIPKWGSPSV
                     LRMGMGCLIAGAVLLAITEIWALQSVLGFIAPMWLVGIGVATAVSVAPNGALRGFDHVA
                     GTVTAVYFCLGGVLLGSIGTLIISLLPRNTAWPVVVYCLTLATVVLGLSCVSRVKGSRG
                     QGEHDVVALQSAESTSNPNR"
                     /name="chloramphenicol efflux MFS transporter CmlA1"
     gene            complement(6234..7072)
                     /locus_tag="I6K60_RS26685"
                     /old_locus_tag="I6K60_26685"
                     /pseudo=""
     CDS             complement(6234..7072)
                     /locus_tag="I6K60_RS26685"
                     /old_locus_tag="I6K60_26685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
                     /name="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
     gene            complement(7130..7738)
                     /locus_tag="I6K60_RS26690"
                     /old_locus_tag="I6K60_26690"
     CDS             complement(7130..7738)
                     /locus_tag="I6K60_RS26690"
                     /old_locus_tag="I6K60_26690"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050382.1"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]; GO:0016791 - phosphatase activity [Evidence IEA]"
                     /GO_process="GO:0016311 - dephosphorylation [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="WP_000050382.1"
                     /translation="MSLALFDFDGTITTRETMPDFVRRSVSRRRLLVGQLLLAPLVLGY
                     KIGILSGTLIRRVIVRFAYSGIPASVLEAQGRDFAQSYLPNTLRGEAMQRIAWHKAQGH
                     KVVVVSGGLEAYLEPWCDSHGVELICSSLQQSDGILTGRYEGRQCVLTEKARLVRERYD
                     LATYPTIYAYGDTPEDLDMLAIATKQYYQWQEVGVAGGR"
                     /name="HAD family hydrolase"
     gene            complement(7834..8676)
                     /gene="estX"
                     /locus_tag="I6K60_RS26695"
                     /old_locus_tag="I6K60_26695"
     CDS             complement(7834..8676)
                     /gene="estX"
                     /locus_tag="I6K60_RS26695"
                     /old_locus_tag="I6K60_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /protein_id="WP_001456218.1"
                     /translation="MKEKVVVDKAISLYTESFGDPAHEPIILIMGAMSSAVWWPDEFCS
                     QLAKMGRYVIRYDHRDTGKSTSYEPGQAPYSVEELADDVVRVIDGYGLEAAHLVGMSLG
                     GFLSQLVALKYPKRVKSLTLIASERLADADPDMPAFDPAIIEYHQRAESLDWSDRDAVV
                     AYQVGAWRINSGTAHAFDAEKIQNIAELNFDRTPNILTTFNHTTLGGGERWLGRLNEIA
                     VPTLIIHGTEDPVLPYVHGLALKDAIRGSKMLTLEGTGHELHHEDWPRIIQAIKGQTS"
                     /name="estX"
     gene            8843..9856
                     /gene="intI1"
                     /locus_tag="I6K60_RS26700"
                     /old_locus_tag="I6K60_26700"
     CDS             8843..9856
                     /gene="intI1"
                     /locus_tag="I6K60_RS26700"
                     /old_locus_tag="I6K60_26700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="WP_000845048.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAFI
                     RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI
                     GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG
                     TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR
                     AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH
                     SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER"
                     /name="intI1"
     gene            <10059..10409
                     /locus_tag="I6K60_RS26705"
                     /old_locus_tag="I6K60_26705"
                     /pseudo=""
     CDS             <10059..10409
                     /locus_tag="I6K60_RS26705"
                     /old_locus_tag="I6K60_26705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /name="DUF3330 domain-containing protein"
     gene            10585..11145
                     /locus_tag="I6K60_RS26710"
                     /old_locus_tag="I6K60_26710"
     CDS             10585..11145
                     /locus_tag="I6K60_RS26710"
                     /old_locus_tag="I6K60_26710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            11149..>12439
                     /locus_tag="I6K60_RS26715"
                     /old_locus_tag="I6K60_26715"
                     /pseudo=""
     CDS             11149..>12439
                     /locus_tag="I6K60_RS26715"
                     /old_locus_tag="I6K60_26715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     ARG             complement(3378..3706)
                     /name="qacL"
                     /gene="qacL"
                     /gene_name="quaternary ammonium compound efflux SMR
                     transporter QacL"
                     /tool="amrfinderplus"
                     /drug_class="QUATERNARY AMMONIUM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6285..7072)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA2"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(4764..6019)
                     /name="cmlA1"
                     /gene="cmlA1"
                     /gene_name="chloramphenicol efflux MFS transporter CmlA1"
                     /tool="amrfinderplus"
                     /drug_class="PHENICOL"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6282..7060)
                     /name="aadA2"
                     /gene="aadA2"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(3877..4655)
                     /name="aadA"
                     /gene="aadA"
                     /gene_name="ANT(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.61"
                     /coverage="98.48"
ORIGIN
        1 ggtaagcaga gtttttgaaa tgtaaggcct ttgaataaga caaaaggctg cctcatcgct
       61 aactttgcaa cagtgccgat tgaagaacta aacgaatccg atgctcggaa actaatggac
      121 acaaactttt ttggagctct ttgggtttgt caggcagtga tgccctattt gaggtcgcag
      181 cgttctggac atatcataca gattacaagc attggcgcta ttatctcggg tccgatgtct
      241 ggtatttaca gtgcaagtaa atttgcgctg gaaggaatga gtgaagcctt agcaaaagaa
      301 gcggaacatt tcggagtgaa actcactatg gtggaaccag gaggatattg gacagattta
      361 tacacttcga tgagttacag caatccatta gattcatacg gtacactgcg cgatgagctg
      421 gcgaaacagt attccgaaga ttcggtcgat agtgatcctt ccttggcagc ggaagccctt
      481 atgaaattag tagccagcaa taatccgcct ttgaggctta tccttggcag catggtatac
      541 gatttggcga tggatacctt aaaagcacgg atggctacct gggaagaatg ggaagctgtt
      601 agccgtgcat cagaaaaggc tattcctgca ccggagagat atggagtata ataccaaaca
      661 tggtttttga ggcaaggacg aaactatcat gaaaattgaa attatggaat ataacccgga
      721 ttggacaaag aattttgaag aagagaaaat aaaattgctc cattttttcg gttctcatgc
      781 cgtagccatt gaacatatcg gaagcacggc gatacccaat caacgggcga aaccggttat
      841 agatatattt attggcgttt cgccttttgc tgaactacct tttatcagcg catttttaat
      901 gcaaaggagt atcaccacac tccgacagat atgacaggca gatatttgtt tgcaaaatat
      961 acaaatgaag tttggaccca taatcttctt gctttggtgt aggtataata cccaaaaacg
     1021 aagtttatgc ggaacaggat aacacctcga aatcggagtt tatatggctt cgtgggggag
     1081 ttatgtgttt gagctttgtc gcattaacgc gaatcagggg ttgacggcag aaatagactg
     1141 gaattacact tcttggagtc ggcgttgccg gaaaattctg attggattag ttgttcgggg
     1201 tgcgaaaaca gtcgtagttc ggggaaaaaa ccgaattttg acccaaaacc gcgaaaaact
     1261 cgaaatgaca gttcctatca gttttcataa tgaattaaaa caaaaaaatt aggctgcaaa
     1321 gatagggaaa atgtgtcaat ttgcgtaagt gataattagt caattaagat aaatgctact
     1381 tttgtaatca aagcaaaatg atatgagcaa gatttttgga atcgtaaata taaccaccga
     1441 tagtttttcc gatggaggac tttatttaga tacagataag gcaattgagc atgctctgca
     1501 tttggttgaa gatggagcag atgtgattga tttgggagcc gcttccagta atcctgatac
     1561 aactgaagtg ggcgttgtgg aagaaatcaa aagactcaaa cctgtcatta aggctttaaa
     1621 agaaaaaggc atttctattt ctgttgatac atttaaacct gaggttcaga gtttttgcat
     1681 agaacaaaag gttgatttta ttaatgatat tcaaggtttt ccttatcctg agatttattc
     1741 aggcttggca aagtcagatt gcaaacttgt gttgatgcac tccgttcagc gaattggtgc
     1801 agctactaaa gttgaaacga atccggaaga ggtttttact tccatgatgg aattttttaa
     1861 agaaagaatt gctgctttag ttgaggctgg tgtaaagcgt gaacgaatta ttcttgatcc
     1921 gggtatgggc ttctttttag gctctaatcc agaaacatct attcttgttt tgaagcgttt
     1981 ccctgaaatt caagaagctt ttaatttgca agtaatgatt gcagtgtcac ggaaatcatt
     2041 cttaggtaaa ataactggaa ccgatgtgaa atctcgttta gcaccaactc ttgcagcaga
     2101 aatgtatgca tacaaaaaag gtgcagatta tctccgcacc catgatgtta agtctttatc
     2161 agatgccttg aaaatatcca aagccctagg ttagttttat ttttttagct ttctaaatga
     2221 atatgctata cttgctctga agttgtgttg gcgtacttga cttaggtctg gatctttcga
     2281 actgttacta agagggtaat catagccaaa cataaactgg aaatgtccta attctaaacc
     2341 tgttccaata cctacactat aatcaagagg acttaaactt ccatcacttc caaaaggttt
     2401 tgtgtacttc tcttgagttt tcgtagctaa atagtagtgt ttgttggtaa atccaaataa
     2461 tccaatattc aatcttgcga ccgggaaagt cctcttgaaa gtaccacaag ttcgcgggat
     2521 ggagttttat cccagctgca tcgagaaagg catgcgcagt gagcgtgctc tcaagctcgc
     2581 catcgccgaa atgtatgtca aaggagtaag tacccgcagg gtctcggata tcgtcgaaat
     2641 tctttgtggc accgaagtca gctcgtccca ggtcagcagg ctggcaaagg agctcgatga
     2701 agagattacg tcttggaagg cgcagcctgt cggacagatt caatacttgg tacttgatgc
     2761 gacctatgaa tcggttcgcg tcggttccca tgtggtcaag caggcgcttc tagtggctat
     2821 tggcgttgat tacagcggga atcggcatat tcttgacgcc gaagtcgcga acagtgaggc
     2881 agaggtaaac tggcgttcct ttctcgaggg tctcgtacga cgagggatgc acggcctgcg
     2941 aatgatcacc agtgatgacc actcaggact gcgcgcggca atcgatgctg tcttccctgg
     3001 aattctgtgg caacgctgcc agtttcatct gcagcagaat gcccactcct acgtcacgaa
     3061 aaaagatgag atcccgctga tagccgctga tattcggaag gtgttcaatc ggaatatgtc
     3121 aagatagttt atgccagatt tttgagaggt ttgcatcaag cagccttcga aagaggacgg
     3181 catgacattg agaccggcaa ggcgtcgtag atacgcactt tcccagtacg ccaccgtaag
     3241 gggtttttct ctttggcagc aagaatggtt tggtttcttt tttcctcggc gccgtaggcg
     3301 tccgactaac acctgaatta agccgcgccg cgaagcggcg tcggcttgaa tgaactgtta
     3361 gatgccagcc cgatcaatgt gcgctgacct tggatagcag atttagaacg gcgacgccac
     3421 taacgataag tcccatgcca acgaacgccc acaagtctag tttctggcca tggaagatcc
     3481 aagcgatagc tgccacaagt acgatgccga ggccagccca aacagcataa gcaatgccga
     3541 ccgggatgga cttgagtgcg agagagagga aatagaacgc aagcccgtag ccagccacaa
     3601 ctacaacaga aggaactaac ttggtgaatc catggctgga cttcagtgcg gaagttgcga
     3661 cgacctcacc aaatattgca atagccagaa agagccagtt cttcacgtgc aatctcctct
     3721 acggtatgaa ggataaatag tggtggctat gagttgccaa aaacagtctt gcggctgtcg
     3781 attttctgtg agcatacgca acgccaaatc tggcatctaa cgcttgagtt aagccgcgcc
     3841 gcgaagcggc gtcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
     3901 tcgcctttca cgtagtgaac aaattcttcc aactgatctg cgcgggaggc caagcgatct
     3961 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
     4021 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
     4081 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     4141 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     4201 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     4261 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
     4321 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     4381 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
     4441 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     4501 acggtcaccg taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg
     4561 gagccgtaca aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact
     4621 acctctgata gttgagtcga tacttcggcg atcaccgctt ccctcatgat gtttaacgcc
     4681 tgagctcagc cgaccgaaac cgcgtagcgg ttttgggtcg gctgcagcga tttgttgggc
     4741 gatagcttgc cacattctct caacgattgg gatttgatgt actttccgca ctttgtagcg
     4801 cgaccacatc atgctccccc tggccgcgag agcccttcac tcgggaaaca caagacagac
     4861 cgagcacgac tgttgcaagg gtcaaacagt acacgacaac cggccaagcc gtgttgcgcg
     4921 gcaacagcga aatgatcaac gttccgatgc ttcctagcag tacaccgccc aagcagaagt
     4981 agactgccgt gaccgttcca gcaacatggt cgaatcctcg aagagcgcca ttgggcgcca
     5041 cagataccgc tgtggcgaca ccaataccca ctagccacat tggagcaata aagcctaaca
     5101 cggactgcaa agcccatatt tcggtgatgg caagcaatac tgctccagct atcaggcatc
     5161 ccattcccat tcgcaagaca cttgggctgc cccacttggg tatcacacgc cccataaaac
     5221 gagccgtaaa caccatggca attgccactg tggcgaacag caggctgaag ccaagctgag
     5281 acacaccttg cctgcccatc attagtccgg gcgcaatgga gaaaaagacg aagaagctac
     5341 ccattccagc ggcgtaacac aacgtgtaca accagaagtt caggcactta acggggagta
     5401 gcagctgcga ccattgcaag cccgcaactc gttgcacccg ggtttcaggc cagaatcgcc
     5461 acgctgctgc agatgcagcg atcatgccca aacctagaaa cgcaaagata gcccgccacc
     5521 caagccacat gtcgacgagc gctccgagca atgggcctac cgccgggacc atggccagca
     5581 tggatccgag tatgccgtaa atgacattac tttcctcgcg acctgcgtaa atgtcacgta
     5641 ctgttgcaaa tgtggaaaca aggcacgccg aggcaccaca agcctgaaga atccgaagcc
     5701 ccagaaagac ttcagccgat gacgtaagag cgaggcccat tgacgccaca acgtaggcga
     5761 ggccacctcc cagtagaacg gggcggcgcc ccagtcggtc cgatagcggt ccaaacaaga
     5821 gctgaccggc accaatcatg accaagtacg ttgtcagcgt aagctgaatt gtgctcgctg
     5881 tcgtaccaag cgcgtttggc ataaacggca ctgctggcaa gtacatgtcc atgccgagtg
     5941 atgccaataa atcgaacggt gataacaaca acaccgtggc ggcaagggag taccgccaac
     6001 taaagttttt tgagctcacg aaaccatctc cttgaacaaa ggatttggcg gcgctcccgt
     6061 agagcaaaag gattcatgag aacgccgcaa caaccgaaaa atgaaggttg ctgcggctta
     6121 cttgtctgcg ttcttggaag tgctcatctg ctgactatct catgattgaa tttgaatcgt
     6181 aacaactttc aagcaactct gcaaggagcc ttattgtgcg cccaacgccg gagttaagcc
     6241 gccgcgcgta gcgcggtcgg cttgaacgaa ttgttagaca tcatttacca actgacttga
     6301 tgatctcgcc tttcacaaag cgaataaatt cttccaagtg atctgcgcgt gaggccaagt
     6361 gatcttcttt ttgtcccaga taagcttgct tagcttcaag taagacgggc tgatactggg
     6421 caggtaggcg ttttattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
     6481 ttgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccggcccagt
     6541 cgggctgcga gttccatagc ttcaaggttt ccctcagcgc ctcgaataga tcctgttcag
     6601 gaaccgggtc aaagaattcc tccgctgccg gacctaccaa ggcaacgcta tgttctcttg
     6661 cttttgtaag caggatagct agatcaatgt cgatcatggc tggctcgaag atacccgcaa
     6721 gaatgtcatt gcgctgccat tctccaaatt gcagctcgcg cttagccgga taacgccacg
     6781 ggatgatgtc gtcatgcacg acaagggtga cttctatagc gcggagcgtc tcgctctcgc
     6841 cagggaaagc cgaagcctcc ataaggtcat tgagcaatgc tcgccgcgtc gtttcatcaa
     6901 gctttacggc cacagtaacc aacaaatcaa tatcgctgta tggcttcagg ccgccatcca
     6961 ctgcggagcc gtacaaatgc acggccagca acgttgattc cagatggcgc tcaatgacgc
     7021 ttagcacctc tgatagttgg ttcgaaattt cgatggtcac cgctaccctc atgatgtcta
     7081 acaccagaat taagccgcgc cgcgaagcgg cgtcggcttg aatgacttgt tagcgcccac
     7141 ctgcgacgcc aacctcttgc cactgatagt actgcttagt agcaatggcc agcatatcca
     7201 ggtcctccgg cgtgtctccg tacgcgtaga tggtcggata tgtcgccagg tcgtagcgct
     7261 cacggaccaa gcgagccttc tccgtcaaga cgcactgccg cccttcatag cggcccgtta
     7321 ggattccgtc cgactgttgc aaggaagaac agatgagctc aacgccgtgc gagtcgcacc
     7381 acggctccaa gtaagcctca agtccgccgg aaacaacgac aaccttgtgc ccctgcgctt
     7441 tgtgccatgc gatgcgctgc attgcctcgc cgcggagggt attgggcagg tagctctgag
     7501 cgaagtcacg accttgggcc tccaggaccg aggccgggat gccgctgtaa gcgaagcgaa
     7561 cgatgacgcg acggatcagg gtgccagaca agatcccaat cttataccca agcacgagcg
     7621 gcgccagaag cagttgtccc acaagcagtc gacgtcggct cacggaccgc cgcacaaaat
     7681 ccggcatcgt ctcgcgagtg gtaattgtgc catcgaagtc aaagagtgcg agagacatgc
     7741 gttgtgggcg ctaacgcctt gctcaccgga aaattgcgag cgcagcgagt aatttttccg
     7801 gtgcagcaac ttgttgtacg gtgcaaccga tagctatgac gtttgcccct taatcgcctg
     7861 gataatcctc ggccagtctt catgatgcaa ctcatgtccc gtgccttcga gtgtcagcat
     7921 ttttgaacca cgaatcgcat ctttcagtgc caacccatgc acataaggaa gtacaggatc
     7981 ctccgtgccg tgaatgatca aagttggtac agctatctca tttaatctcc cgagccatct
     8041 ctcgccacca cctaaagtag tgtggttgaa tgttgtcagg atattcggag tgcgatcaaa
     8101 atttaactca gcgatgtttt gtatcttctc agcgtcaaaa gcatgcgcag tacctgagtt
     8161 gattcgccac gctccgacct gatacgcgac gacagcatct ctatcagacc aatccagcga
     8221 ttccgcccgt tggtgatact caatgatggc aggatcaaaa gcgggcatat ccggatctgc
     8281 atctgcaagc cgttctgaag caatcagcgt caagctcttc acacgtttcg gatacttgag
     8341 agctacaagc tgggaaagaa atccccccaa agacatgccg actaaatgag cagcttccag
     8401 accataacca tcaatgacgc gaaccacatc atctgctaat tcttcaacgg aatatggagc
     8461 ctgacctggc tcatagcttg ttgatttccc ggtatcacgg tggtcgtacc ggatcacata
     8521 gcgacccatt ttggcaagtt gggaacaaaa ctcatcaggc caccacaccg cagacgacat
     8581 tgcccccatg atcagaataa tgggttcatg ggccgggtcg ccgaatgatt cggtatagag
     8641 tgaaatcgct ttatcaacaa ctaccttttc tttcatactt aaattgcctt cgagttctac
     8701 gtacaacttt gttttagggc gactgccctg ctgcgtaaca tcgttgctgc tccataacat
     8761 caaacatcga cccacggcgt aacgcgcttg ctgcttggat gctcgaggca tagactgtac
     8821 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     8881 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac
     8941 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     9001 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     9061 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     9121 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     9181 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     9241 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     9301 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     9361 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     9421 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     9481 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     9541 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     9601 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     9661 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     9721 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     9781 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     9841 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     9901 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     9961 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
    10021 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
    10081 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
    10141 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
    10201 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
    10261 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
    10321 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
    10381 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acttgatgtc aggcagggcc
    10441 gcgccgcttc gtcagaatag agtctgcttt cccatttttt gacacatgcc cgcgaaggtt
    10501 atagatttca gcctgacaga aatgggcttt gaggcacaac ggaacagaaa gtgcacttaa
    10561 gccgccttca accaaggaga catcgtgcag gggcaccgca tcggctacgt ccgggtcagc
    10621 agctttgacc agaacccgga acgccagctg gaacaaaccc aggtgagcaa ggtgttcacc
    10681 gacaaggcat cgggcaagga cacccagcgc ccccagctcg aagcgctgct gagcttcgtc
    10741 cgcgaaggcg atacagtggt ggtgcacagc atggatcggc tggcccgcaa cctcgatgac
    10801 ctgcgtcgct tggtacagaa gctgactcag cgcggcgtgc gcatcgagtt cctgaaggag
    10861 ggcctggtgt tcactggcga ggactcgccg atggccaacc tgatgctgtc ggtgatgggg
    10921 gccttcgctg agttcgagcg cgccctgatc cgcgagcggc agcgtgaggg catcgccttg
    10981 gccaagcagc gtggcgcgta ccggggccgc aagaaagccc tgtccgatga gcaggctgct
    11041 accctgcggc agcgagcgac ggccggcgag cccaaggcgc agcttgcccg cgagttcaac
    11101 atcagccggg aaaccctcta ccagtacctc cgcacggacg actgacacat gccgcgtcgc
    11161 ttgatcctct cggccacgga gcgggacacc ctgcttgcgc tgccggaaag ccaggatgac
    11221 ctgatccgct actacacctt caacgactcc gacctgtcgc tgatccgcca gcgacgcggc
    11281 gacgccaacc gcctcggctt cgccgtgcag ctctgcctgc tgcgctaccc cggttacgcg
    11341 ctgggaaccg acagcgagct gcccgagccg gtcatcctgt gggtggcgaa gcaagtccag
    11401 gccgagccgg cgagctgggc aaagtacggc gagcgcgacg tgacccgtcg cgagcatgcc
    11461 caggaactgc gcacctacct gcaactggcc ccgttcggcc tgtccgactt ccgcgccctg
    11521 gtgcgcgagc taaccgagct ggcccagcag accgacaaag gcttgctgct ggccggtcag
    11581 gccctggaga gcctacggca gaaacgacgc atcctgccgg cgctgagcgt gattgaccgg
    11641 gcctgctcgg aagccattgc gcgagccaat cggcgggtct accgcgccct ggtcgaacca
    11701 ctcacggact cgcatcgggc caagctggac gagctgttga agctcaaggc cggcagcagc
    11761 atcacctggt tgacctggct gcgccaggca ccgctgaaac ccaactctcg gcacatgctg
    11821 gaacacatcg agcggctgaa gacatttcag ttggtggact tgcccgaagg cctgggccgg
    11881 cacatccacc agaaccgcct gctcaagctg gcccgcgagg gtgggcagat gacgcccaaa
    11941 gacctcggta agttcgagcc gcagcgccgc tacgcgaccc tggccgccgt ggtgctggag
    12001 agcaccgcga ccgtgatcga tgagctggtc gatctgcatg accgcatcct ggtcaagctg
    12061 ttcagcggcg cgaagcacaa gcatcagcag cagttccaga agcagggcaa ggcgatcaac
    12121 gacaaggtgc gcctgtactc caggatcggc caggcgctgc tggaagcgaa ggaaagcggc
    12181 agcgacccct atgccgccat cgaggcggtg attccctggg acgagttcac cgagagcgtc
    12241 agcgaggccg agctgctggc ccggccggaa ggcttcgacc acctgcacct ggtcggcgag
    12301 aacttcgcca ccctgcgccg ttacacgccg gccttgctgg aggtgctgga actgcgcgcc
    12361 gcgccggccg cgcaaggcgt gctggcagcc gtgcagaccc tgcgtgagat gaacgccgac
    12421 aacctgcgca aggtgccgg
//
