LOCUS       NZ_CP070186             4515 bp    DNA     linear   CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1330 plasmid unnamed7.
ACCESSION   NZ_CP070186
VERSION     NZ_CP070186.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357472
            Assembly: GCF_016903515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 635.94x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:11:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070186.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1330"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1330"
                     /db_xref="taxon:573"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..242
                     /locus_tag="I6K60_RS26610"
                     /old_locus_tag="I6K60_26610"
     CDS             <1..242
                     /locus_tag="I6K60_RS26610"
                     /old_locus_tag="I6K60_26610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_204724672.1"
                     /translation="SEQESARGSFQGETPGIFIVLSGFATSDLSVCFCDARQGGGAYGK
                     TPAARPSSCSWSFAHMFLLALSPDSVDNRITVFE"
                     /name="hypothetical protein"
     gene            complement(553..744)
                     /locus_tag="I6K60_RS26615"
                     /old_locus_tag="I6K60_26615"
     CDS             complement(553..744)
                     /locus_tag="I6K60_RS26615"
                     /old_locus_tag="I6K60_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165986.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            807..1130
                     /locus_tag="I6K60_RS26620"
                     /old_locus_tag="I6K60_26620"
     CDS             807..1130
                     /locus_tag="I6K60_RS26620"
                     /old_locus_tag="I6K60_26620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001677263.1"
                     /translation="MLTIRVSDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     SPPLLRHLAAIGNNLNQTARKVNSGHWSSIDRVHVVAALMAIEGELRQLRQAVREQGGR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1339..>1776
                     /locus_tag="I6K60_RS26985"
                     /pseudo=""
     CDS             1339..>1776
                     /locus_tag="I6K60_RS26985"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1808..2368
                     /locus_tag="I6K60_RS26630"
                     /old_locus_tag="I6K60_26630"
     CDS             1808..2368
                     /locus_tag="I6K60_RS26630"
                     /old_locus_tag="I6K60_26630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067488.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKQQLDTLSTTVSTQLSTTEAELSRQQKNLLWQVIKGRVLFPALTA
                     LSVTGGIFLGCWGLIQWQESRIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            2375..2608
                     /locus_tag="I6K60_RS26635"
                     /old_locus_tag="I6K60_26635"
     CDS             2375..2608
                     /locus_tag="I6K60_RS26635"
                     /old_locus_tag="I6K60_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137388.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHAVTQQNNA
                     ILNERVVNLSQQVQLLAGQVDRLSRLFITNNR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3337..4176)
                     /locus_tag="I6K60_RS26640"
                     /old_locus_tag="I6K60_26640"
     CDS             complement(3337..4176)
                     /locus_tag="I6K60_RS26640"
                     /old_locus_tag="I6K60_26640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001354646.1"
                     /translation="MLTLFIFFVLLIAACFFCFAPPRRGYDRNEIIPYKIKLSINKYRL
                     YIYSSGKVRQYLLFLVILSLYYSIAEPFKSELIKNISYSLMAAFIFDTGLNFSKENITK
                     GVISTRWHNDLYSSFERMKAINKIYYPSNKEINTEGLSKAITSSLFNDDANSFAKRDFR
                     LMWDLSSEKYLSYKEIIIRKGDKLDAVCLRFINDDYKFLVNFNRDEEVFKYFPSIMQPS
                     LKTYRALSRLVNSIKDPSRFKFTTESLEMELLEYLELRNELFNDIEEVMGSYAQRAP"
                     /name="hypothetical protein"
     Typing          381..465
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.03e-37"
     Typing          1121..2706
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.811"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtctgttt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcggcgc ggccttcttc ctgttcctgg tcttttgctc
      181 acatgtttct tttggcgtta tcccctgatt ctgtggataa ccgcatcacc gtctttgagt
      241 gagctgatac cgctcgccgc agccgaacga ccgagcgtag cgagtcagtg agcgaggaag
      301 cggaagagcg cctgatgcgg cattttctcc ttacgcatat gtgcggtatt tcacaccgca
      361 tatggtgcac ttttcttata atccgcactg atgccacata gttaagccag tatacactcc
      421 gctatcgcta cgtgactggg tcagggctgc gccccgacac ccgctaaaac ccgctgacgc
      481 gccctgacgg gcttgtctgc tcccggcatc cgctcacagg caagctgtga acgtctccgg
      541 gagctgcatg tttcagttgc tttcaccgtc atcgccgaag cgtgcgaggc aactacggta
      601 gagttcgtcg gcgtggtcgt gaagtgattc gcagatgtct gcctgttcgt ccgcgtccag
      661 ctcgttgagt ttttccagca gggtcagcgt ctggcttctg ataaagcggg ccatgttaag
      721 ggctgttttt tcctgtttgg tcatggtctt aaggctcctt aataggggat tttttagggg
      781 gaatgatacc gatgaaacgc gagaagatgc tcacgatacg ggttagcgat gaggagcacg
      841 cccggttact ggaacgctgc gagggtaaac gactggcaga atggatgcgg cgggtctgtc
      901 tgggggagcc ggttgcgcgc acaggaaaac tgccgacact atcgccgcca ttgctgcggc
      961 atctggctgc catcgggaac aatctgaacc agacagcccg gaaggtgaac agcggccact
     1021 ggtcttcgat tgaccgggtt catgtggtgg ccgcactgat ggccatcgag ggtgaattgc
     1081 gccagttgcg gcaggctgtc agggaacagg ggggacggga tgatagttaa atttcatgcc
     1141 agggggaaag gcggtggaag tggcccggtc gattacctgc tggggcggga gcgtaaccgc
     1201 gaaggcgcaa cggtgctccg gggtaacccg gaagaaatcc gggaactcat tgatgccacg
     1261 ccattttcga agaaatacac gtcaggcgtt ctgtcgttcg cggagaagga gttaccgccg
     1321 ggtgaacgcg aaagagtgat gacgagcttt gagcgtgttc ttatgcccgg tctcgaaaaa
     1381 gaccagtaca gcattctctg ggtggaacac caggataagg ggcggctcga actgaatttt
     1441 gtcattccga acatggaact acagagcggt aagcgcctcc agccgtacta cgaccgtgcg
     1501 gacaggccga ggataaatgc ctggcagacg ctggttaatc atcattacgg gctgcgtgac
     1561 ccgaacgcac cggagaacag acggacgctt gtcacaccga ataatctgcc aaaagcgaaa
     1621 caggaggccg cagaagcgat tacacggggt cttgaggcgc tttaccgtgc cggagcaata
     1681 aaaacgcgtc aggatgtcac tgaggcgctc acagcggcag gttttgaggt ggtgaggaca
     1741 accagaagca gtatcagtat tgccgacccg gagggggggc ggaatctcag gctaaagggg
     1801 gcgctgtatg agcaatcttt tacaaatggg aacagacttc gagaagaaac tgaaagagcg
     1861 agccgcttct accgagaaca tgctgaacag cgagttcagg aagctagaag agtctgtaga
     1921 caaggcactg agcttaaaca ggcagaaaat cagagacgct atcagcgaac atacaacatc
     1981 cgtgaaacag caactggata cgctgagcac caccgtgagc acgcagctca gcaccacaga
     2041 ggccgaactg tcccggcagc agaaaaacct gctctggcag gtgatcaaag ggcgggtact
     2101 gtttccggcg ctgaccgcgc tcagcgtcac gggcggcatt ttcctggggt gctgggggct
     2161 gattcagtgg caggaaagca ggattgcgaa aaatatcttg accatcagag agcaggaaaa
     2221 cacgctggcg aaactcgagg caaaaacctg gggggtgacg ttcgtcaatg gcgagaacgg
     2281 gaaatttctg gtgctgccgg acggggtgaa aggggaaaac acctggacgg tgggcgacaa
     2341 gaacgccgtg aggctggtca gggagtaaac gagtatgacg gaactggaag ggcatttgct
     2401 gaacgcctta gagcatttgc aacaggacta tatgcggcgg ctgaacgagt gggagagcgc
     2461 cttcgcagaa ttgcagaaga tgcacgcggt tacgcaacag aacaacgcga tactgaacga
     2521 gcgtgtcgtg aacttgagtc agcaggtgca gctattagcc ggacaagtcg accgcttgag
     2581 tcggttattt atcacgaaca acagataagg gatgaacagg agcgtcaggc gcaggagctt
     2641 gcccggcgct ggcatgaaga gcaggaaccc gaagcgcgga tctggcgtgg cccgtcaatg
     2701 gggatgtgac cggggtgccc cctgaaatcg agccagtttg tggcacgccc tacccggatg
     2761 gcgccaatgt taatgtacgt acattagcat taacaatatc caaacctccc cattttgccc
     2821 cgttcgacgg ctggaacgtg cgaaactgat cgtcattcag tttcgcgcat aactatgcat
     2881 gaggttaaaa tttaccaggc gcgatcgcgg cagtttttcg ggtggtttgt tgctgttttt
     2941 acctgtctgc tgccgtgatc gcgctgaacg cgtttaagcg gtacgcgcaa tgcgacgtta
     3001 tggtaaatac ggagtatttt aattgcggga attaaaacaa caaaggcccg ctgtactcta
     3061 tacagcgggc ctttgttgta tatctctgtc gtttgtcccc gggggggaag cattactgct
     3121 aacaagtccc gtgagaaaac tgcgcaatcg tattgcgcac catgaaccta ttctcaaccg
     3181 caatctggag gatgattttg ctacgataaa gagaattatc gcgtatcggt gtcagcattc
     3241 attggagtgg atgctgaaaa accaggtgtt actccctcta cttacactta aacctctcta
     3301 aaaatataag taagtactat cgagggctat catgaactat ggtgccctct gagcatagct
     3361 acccattact tcttctatgt cattgaataa ttcatttctt aactctaagt attctagtaa
     3421 ctccatttct aatgactctg tcgtgaactt aaatctagaa ggatctttga ttgagtttac
     3481 tagtcttgat aacgctctat atgttttgag tgaaggttgc attatagatg gaaagtattt
     3541 gaaaacctct tcatctctgt tgaaattaac taaaaacttg tagtcatcat ttataaatct
     3601 caagcataca gcgtcaagct tatcgccttt tcttattatt atttctttat aggaaagata
     3661 tttttcagaa gataagtccc acatcaagcg aaaatctctc tttgcgaacg agttcgcgtc
     3721 atcattgaaa agagatgatg tgatggcttt ggatagacct tctgtgttta tctctttgtt
     3781 ggatgggtag taaattttat taattgcttt cattctctca aatgatgaat ataggtcatt
     3841 atgccacctt gttgaaatca cccctttagt tatattctcc ttgctaaaat taagccctgt
     3901 atcaaaaata aatgcagcca tcagtgaata tgatatgttt tttattaact cagacttaaa
     3961 tggttccgct atggagtagt acagtgataa aataactaaa aataaaaggt attgtcttac
     4021 ctttcccgat gagtatatgt aaaggcgata tttatttatt gatagtttta ttttgtatgg
     4081 aatgatctca tttctgtcat atcctcttcg tggtggagca aaacaaaaga aacatgcagc
     4141 tattaataaa acaaaaaaga tgaacagtgt aagcaagttc ggctcctgtg tatgctcact
     4201 atgcatgatt gtcttgagat ctaacgatcc tgaaggtagt cttgtccgta aacgaaaaaa
     4261 ccgcccagca aggcggcttt tcgaaggttc tcagagcagc aactctttga accaaggtaa
     4321 ctggcttgga ggagcacagc caccaaatct gtcctttcag tttagcctta accggcgcat
     4381 aacttcaaga ctaactcctc taaacggtta ccaatggctg ctgccagtgg cgtttttacg
     4441 tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     4501 acggggggtt cgtgc
//
