LOCUS       NZ_CP070184             4981 bp    DNA     circular CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1330 plasmid unnamed6,
            complete sequence.
ACCESSION   NZ_CP070184
VERSION     NZ_CP070184.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357472
            Assembly: GCF_016903515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 635.94x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:11:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070184.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1330"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1330"
                     /db_xref="taxon:573"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(4776..4981,1..28)
                     /locus_tag="I6K60_RS26420"
                     /old_locus_tag="I6K60_26420"
     CDS             join(4776..4981,1..28)
                     /locus_tag="I6K60_RS26420"
                     /old_locus_tag="I6K60_26420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001360356.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSGQTERLSQLYSENWK"
                     /name="MbeD family mobilization/exclusion protein"
     gene            670..1845
                     /locus_tag="I6K60_RS26425"
                     /old_locus_tag="I6K60_26425"
     CDS             670..1845
                     /locus_tag="I6K60_RS26425"
                     /old_locus_tag="I6K60_26425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011217640.1"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="WP_000893405.1"
                     /translation="MLEQVKDFLGDSYNPQTGEFNAEKARAEAEEHKDKFNIILCGATG
                     VGKSSLVNAVFGEEIVKSGVGSPVTQHLEKITVPRKGLVLWDTKGIESKDYENTINQLK
                     NDIFHAFNDSSHEDDVPHLGWVCIDSSGARIEERDIEIIGILREKEIPVVVVFTKILGD
                     ESADFIEAAISEINAHHGDFINGRYVKVNSRERKILNISIPVSGLDDLVDISFKALPES
                     KSRARQALKKAQMVKMEERLEAMKDSAKLIVHTSSVAAGVVGATPVPGSDAPLIAGVQS
                     TMIYKINSEFELDITTSASASIISGILGVTAVAQVGKTIVSNVLKFIPGVGSVVGGAIS
                     AATAVGLTEAIGHAYIQVLASYYDKTTGKVLLPESVSVVLDLFKKYFTYKK"
                     /name="GTPase"
     gene            complement(2165..2335)
                     /locus_tag="I6K60_RS27065"
     CDS             complement(2165..2335)
                     /locus_tag="I6K60_RS27065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_162708437.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPERHDLSPLAAATGNWIRRALMF
                     NQYYRVLEVMA"
                     /name="replication initiation protein"
     gene            complement(2994..3185)
                     /locus_tag="I6K60_RS26430"
                     /old_locus_tag="I6K60_26430"
     CDS             complement(2994..3185)
                     /locus_tag="I6K60_RS26430"
                     /old_locus_tag="I6K60_26430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165987.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGETL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3250..3573
                     /locus_tag="I6K60_RS26435"
                     /old_locus_tag="I6K60_26435"
     CDS             3250..3573
                     /locus_tag="I6K60_RS26435"
                     /old_locus_tag="I6K60_26435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3782..>4213
                     /locus_tag="I6K60_RS26970"
                     /pseudo=""
     CDS             3782..>4213
                     /locus_tag="I6K60_RS26970"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4251..4769
                     /locus_tag="I6K60_RS26445"
                     /old_locus_tag="I6K60_26445"
     CDS             4251..4769
                     /locus_tag="I6K60_RS26445"
                     /old_locus_tag="I6K60_26445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_204724669.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNDEFSRLGESVSEAVTSNE
                     TKIKGAIALFTTSTEESLKKHREGVKEAMMQHRKDVLKLAGNTGVMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRVQEETLRKLNAKTWGVEFVQGGGRKFLVIPQGMSAEVTHYQ
                     GKDWIQLTE"
                     /name="MbeB family mobilization protein"
     Typing          2837..2916
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="94.0"
                     /evalue="1.83e-34"
     Typing          complement(2264..2808)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.333"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          3564..4981
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.406"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 ccagttatac agcgagaact ggaaataagg gaggaacggc tgatacagga gcgcgaacat
       61 gcgttatccc tggaacggga acgccagctg gaaatacagg aacgcacact ggatggtcct
      121 tcgttgtcgc tgtgatggcg aaactataaa aaatggcagg ttcagtggat tttgacggac
      181 taatgtagtc tgcaccatct ggttgcatag gtattcatgc ggttaaaatt tatcaggcgc
      241 gatcgcggca gtttttcggg tggtttgttg ccatttttac ctgtctgctg ccgtgatcgc
      301 gctgaacgcg tttaagcggt gcgcataaaa tggggttatg ttaaatatga ttgatatcta
      361 tagatatcag actaactatt tattttgtac atggtgttaa aattggtaat gattagaatg
      421 gctatgactt ataaatgttt atcatgccat tggtgtaaaa ctattcctaa atgtagtgat
      481 gctttagttg atggagtgag cgcctttagt cggtgcccaa attgcgattc tattttggtt
      541 gatgaaaaaa gagccaacat aattaactcg gttattgcaa atataatatt caaaataaaa
      601 tcgtaaatga ttttattatt tgtgtgatcg tttgcgtttt tgttggtttg ttattatgtg
      661 gaggttaaaa tgttggagca agttaaagat tttttaggtg attcctataa tccacaaact
      721 ggtgagttta atgcagaaaa ggcaagggca gaggctgagg aacataagga caaatttaat
      781 ataattcttt gtggagctac aggagttggt aaaagttctt tggttaatgc agtttttggc
      841 gaggagattg ttaagtcagg agtgggaagt ccagttaccc agcatttaga aaaaatcact
      901 gttccaagaa aagggctggt gttgtgggat acaaaaggta ttgagtcaaa agattatgaa
      961 aataccataa atcagttaaa gaatgatatt tttcatgcat tcaatgactc atctcatgaa
     1021 gacgatgtgc ctcatttagg gtgggtttgt attgattcgt caggtgcaag gatagaagaa
     1081 agagatattg agataattgg tattttaaga gaaaaagaaa tacctgtagt tgttgtgttt
     1141 acaaaaatac ttggcgatga gtctgccgat tttatagagg cagccattag tgaaattaat
     1201 gctcatcatg gtgattttat taatggtagg tatgtaaagg taaattcaag agagcggaaa
     1261 atacttaata tttctatccc tgttagtggt cttgatgatt tagtagatat tagttttaag
     1321 gcgttaccag aatctaagag tcgtgccagg caagccttaa aaaaggctca aatggttaaa
     1381 atggaggagc gactggaggc catgaaggat agtgcaaaat taattgttca tacatcatct
     1441 gtagctgctg gggtggttgg tgcaactcct gtgccaggct ctgatgctcc gcttattgct
     1501 ggagtgcaga gtacaatgat atacaagatt aattctgaat tcgaacttga tataacaaca
     1561 tcagcatcag cgagtattat tagtgggata cttggagtta ccgcggtagc acaggtgggt
     1621 aagactattg tatctaatgt tttaaaattc attcctggtg tgggttccgt tgttgggggg
     1681 gcaatatcag cagctacagc agttggtctt actgaggcta ttggtcatgc atatattcaa
     1741 gttctggcta gttattatga taaaacgact gggaaagtcc tccttccaga gagtgttagt
     1801 gtagttttgg atttgtttaa gaaatacttc acatataaaa agtaatttat gggtgaatat
     1861 gttttaccac tctggtgata aatatattct taatacggcc tccagttatg gatttagttg
     1921 atacaactta aacgaagtta tgtgagatag aggattgctt cgtttgtatt tgcatcaagt
     1981 tggcatagat gaaaaggatc tccttgagat cctttgaaag gcgtcgtaat ctcttccgct
     2041 gtaaacgaaa aaaccgcctg gggaggcggt ttttcgaagg ttaagtcagt tggggactgc
     2101 ttaaccgggt aactggcttc agcagagcac agataccaaa actgtccttc cagtgtagcc
     2161 gtagttaggc catcacttca agaactctgt agtactgatt aaacatcaat gctctgcgaa
     2221 tccagttacc agtggctgct gccagtggcg ataagtcgtg tctttccggg ttggactcaa
     2281 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     2341 ccagcttgga gcgaacgacc tacaccgaac tgagatacca acagcgtgag ctatgagaaa
     2401 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2461 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2521 ggtttcgccg cctctgactt gagcgtcgat ttttatgatg ctcgtcaggg gggcggagcc
     2581 tatggaaaaa cgccaacggc gcagcctttt ccgggttctc gttttttgct cacatgtttc
     2641 ttttggcgtt attccctgat tctgtggata accgcatctc cgcttttgag tgagcagaca
     2701 ccgctcgccg cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc
     2761 gccggaacgt gcattttctc cttacgcatc tgtgcggcat ttcacaccgg acatggtgcg
     2821 ctttccatac aattcgcact gatgccgcat agttaagcca gtatacactc cgctatcgct
     2881 acgtgactgg ttcagggctt cgccccgaaa ccccctgacg cgccctgacg ggcttgtctg
     2941 ctcccggcat ccactcacag gcaagctgtg agcgtctccg ggagctgctt gtgtcagagg
     3001 gtttcaccgt catccccgaa gcgtgcgagg cagctgcggt aaagctcatc ggcgtggtcg
     3061 tgaagagatt cacagatgtc tgcctgttca tccgcgtcca gttcgttgag cttctccagc
     3121 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3181 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     3241 cgcgagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgc
     3301 tgtgagggta agcaactggc agtatggatg cggcgggtct gcctgggtga gcctgtcgcc
     3361 cgttccggga agctgccgac actggcgccg ccgttactgc gtcaactggc cgccatcggg
     3421 aataacctga atcagacagc gaggaaggtg aacagcgggc agtggtcttc cggcgaccgg
     3481 gttcaggtgg tggccgcact gatggccatc ggggatgagc tgcgccggct gcgtctggcc
     3541 gtcagggaac agggggcgcg ggatgatagt taaatttcat gccaggggaa aaggtggtgg
     3601 cagtggtccg gttgattacc tgctggggag ggagcgtaat cgcgaaggcg caacggtgct
     3661 tcagggtaat ccggaagaag tccgggaact catcgatgcc acgccatttg cgaagaaata
     3721 cacgtccggt gttctgtcgt ttgcggagaa agaactgcca ccgggaggac gtgaaaaagt
     3781 gatggcgagt tttgagcgtg tactgatgcc cggtctcgaa aaaaatcagt acagcatcct
     3841 gtgggtggag caccaggaca agggacggct tgagctgaat tttgtcattc cgaacatgga
     3901 gctacagacc ggaaaacgcc tccagccgta ctacgaccgg gcagacaggc cgagaattga
     3961 tgcctggcag acactggtga atcaccacta cgggctgcat gaccccaacg ccccggagaa
     4021 tcgccggacg ctgacactcc ctgataacct gccggaaacg aaacaggcgc ttgctgagag
     4081 cgttacacga ggcctggagg tgctttacca ttccggagag ataaaaggcc gtcaggatgt
     4141 gattcaggcg ctcacagagg tggggcttga agtggtcagg gtgacgcgaa gcagtatcag
     4201 cattgcagac ccgaacggcg ggaggaatat cagactgaaa ggagcgtttt atgagcaatc
     4261 ttttacggac gggcgcggag ttcgagaaaa agctgaaaga gagagccgaa tctacagaga
     4321 aaatgctgaa cgacgaattc agcaggctcg gcgaatctgt aagcgaggct gtgacctcaa
     4381 acgagacgaa aatcaaaggc gctatagccc tgttcacaac gtcgacagag gaatcgctga
     4441 aaaaacaccg ggaaggggtg aaagaggcga tgatgcagca ccggaaggac gtgttaaagc
     4501 tggcagggaa tacgggcgtg atgttactgg ggatagtctt tctcctgttt accgcgagtg
     4561 gcgggacgct ctggtatctc ggcgggagga tacagacgaa cctggaagaa atcagggtac
     4621 aggaagagac attgcggaaa ctgaacgcga agacatgggg cgtggagttt gttcagggcg
     4681 ggggaagaaa atttctggtc attccgcaag ggatgtcagc agaggtgacg cactatcagg
     4741 ggaaggactg gatacaactg acggagtgat acggaatgac agagctggaa acacatttgc
     4801 tgaacgcctt agagcagctg caacaggact atatgcagcg gctgaacgaa tgggagagcg
     4861 ccttcgtgga attgcagaag atgttttcgc ttacgcaacg ggacaacgcg atgctgaacg
     4921 agcgggtcat gcagttgagt cagcaggtgc agcacttgag cgggcagaca gaacgcttga
     4981 g
//
