LOCUS       NZ_CP070181             2579 bp    DNA     circular CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1330 plasmid unnamed9,
            complete sequence.
ACCESSION   NZ_CP070181
VERSION     NZ_CP070181.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357472
            Assembly: GCF_016903515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 635.94x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:11:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070181.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1330"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1330"
                     /db_xref="taxon:573"
                     /plasmid="unnamed9"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(2027..2579,1..92)
                     /locus_tag="I6K60_RS26370"
                     /old_locus_tag="I6K60_26370"
     CDS             join(2027..2579,1..92)
                     /locus_tag="I6K60_RS26370"
                     /old_locus_tag="I6K60_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(474..710)
                     /locus_tag="I6K60_RS26375"
                     /old_locus_tag="I6K60_26375"
     CDS             complement(474..710)
                     /locus_tag="I6K60_RS26375"
                     /old_locus_tag="I6K60_26375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012772745.1"
                     /translation="MARLTNLTPAEKKFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IELQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     gene            <1778..1933
                     /locus_tag="I6K60_RS26380"
                     /old_locus_tag="I6K60_26380"
                     /pseudo=""
     CDS             <1778..1933
                     /locus_tag="I6K60_RS26380"
                     /old_locus_tag="I6K60_26380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707212.3"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             1992..2578
                     /name="QnrB10"
                     /gene="QnrB10"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="99.49"
                     /coverage="86.73"
     ARG             4..92
                     /name="QnrB19"
                     /gene="QnrB19"
                     /gene_name="quinolone resistance protein (qnr)"
                     /tool="rgi"
                     /drug_class="fluoroquinolone antibiotic"
                     /identity="100.0"
                     /coverage="13.55"
     Typing          complement(876..1049)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          1028..1519
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 atattcgggg tgttgattta caaggcgtta agttagacag ctaccaggca tcgttgctca
       61 tggagcggct tggcatcgct gtgattggtt agttcagcgt ttacaccggg agatgcgttt
      121 gacgatttac ttttacccgc gatcatcgcg tttattgagt ttattattta tctatgcgat
      181 tccgatgaaa aattcgcacg aaaatatggc taactcatag caaaaaaaaa gccccctaag
      241 ggcttttttt tgtacctgac ggtcagaccg attaaacata acatcccttg cccgcacccc
      301 cgcaggcgcc ctcagcgcaa tcctgctgca tcagggccgt ttccagcacc tttcctcacc
      361 agtatcccgg aaaaacgccc ctgatgattt ctgcgcgact gcatggctat gcacgcaggt
      421 gaatggcagg tgatttcgca gcgtcagcac cactaaaccg ccaaaagcca aaatcagcgg
      481 cgcggcgcgc gcggacgtga ccaggcaaaa tcggactgct gccgctcatc ctcacgtaat
      541 gcacgctggc ggtcggcgta acgctgcaac tcaatttgcg cacgcgctct gttcaggacg
      601 atgtgccggt taggctgatt tagcttttta cctgacgctc tttcagctgc ggcaacagca
      661 tcatcaagaa attttttctc tgcaggcgtc aagtttgtca gtctggccat aacgttctcc
      721 agtcagaata aatcagcaca gtctgccaga cacagtagaa acgatgaagc gccagcgacg
      781 gtgaaaggca gtcagaaacg gtgaacggga gcggatttta atccggtgcc gtgaggcgtt
      841 ggacaagccg caggcgcgtc attagctttt agccggtttc ggggcgcagc cctgaaccag
      901 tcacgtagcg ctagcggagt gtatactggc tgactatgtt ggcacggatg tggaaatcag
      961 tgaagtgctt catgtagcag gagaaaaaag gcggcaccag cgcgtcagca ggatatgtag
     1021 tacaggatat attccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1081 cgagcggtgc cggcttacag gcggggcgga aatttcctgg gagataccag gaaggagctt
     1141 aacagggaag agataaggcc gcggcgaagc cgtttttcca taggctccgc ccccctgaca
     1201 aacatcatga aatctgacgc tcaaatcagt ggtggcgaaa cccgatgcgg cttaaagata
     1261 ccaggcgttt ccccctggtg gctccctcgt gctcccccgc attcctgcct ttcggtttac
     1321 cggtgtcatt ccgctgttat ggccgcgttt atctcattcc acgcctgaca ctcagttccg
     1381 cgaaggcagt tcgctcctgg atggactgta tgcacgaacc ccccgttcag tccgactgct
     1441 gcgccttatc cggtaactat catcttgagt ccaacccgga aagacacgac ttatcgccac
     1501 tggcagcagc cactggtaac tgatttagag gagttagtct tgaagttatg cgcctgttaa
     1561 ggctaaactg aaagaacaga tttggttact gcgctcctcg taagccagtt accacggttc
     1621 aaagagttgc tgctctgaga accttcgaaa aaccgccttg cagggcggtt ttttcgttta
     1681 cagagcaaga gattacgacg ccacagaaag gatctcaaga agatccttca ccgtaatgga
     1741 aatctatcca atccgagcac ataggataca catcgttata acgctggatc tgcgtgaatt
     1801 ccagcagcag caagagaaag actttctaca aaccagctta cagcaggcta aatttaacca
     1861 gaaaaaagcc gctgaattac tgggtctgac ttatcatcag ctacgtgcct tgctgaagaa
     1921 acaccagatc taatacgcat ttgcaggtgt catactggcg ggtttgacgc ataacgtcat
     1981 aaggtttacc atggcgtcat tactgtataa aaacacaggc atagatatga ctctggcatt
     2041 agttggcgaa aaaattgaca gaaatcgctt caccggtgag aaagttgaaa atagtacatt
     2101 ttttaactgc gatttttcag gtgccgacct gagcggcact gaatttatcg gctgccagtt
     2161 ctatgatcgc gaaagtcaga aagggtgcaa ttttagtcgc gcaatgctga aagatgccat
     2221 tttcaaaagc tgtgatttat caatggcaga tttccgcaac gtcagtgcct tgggcattga
     2281 aattcgccac tgccgcgcac aaggcgcaga tttccgcggt gcaagcttta tgaatatgat
     2341 caccacgcgc acctggtttt gcagcgcata tatcactaat actaatctaa gctacgccaa
     2401 tttttcgaaa gtcgtgttgg aaaagtgtga gctgtgggaa aaccgctgga tggggactca
     2461 ggtactgggt gcgacgttca gtggttcaga tctctccggc ggcgagtttt cgactttcga
     2521 ctggcgagca gcaaacttca cacattgcga tctgaccaat tcggagttag gtgacttag
//
