LOCUS       NZ_CP070179             4062 bp    DNA     circular CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1330 plasmid unnamed8,
            complete sequence.
ACCESSION   NZ_CP070179
VERSION     NZ_CP070179.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357472
            Assembly: GCF_016903515.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 635.94x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:11:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,373
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 5,139
            CDSs (with protein)               :: 5,139
            Genes (RNA)                       :: 128
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 107
            CDSs (without protein)            :: 106
            Pseudo Genes (ambiguous residues) :: 0 of 107
            Pseudo Genes (frameshifted)       :: 42 of 107
            Pseudo Genes (incomplete)         :: 56 of 107
            Pseudo Genes (internal stop)      :: 20 of 107
            Pseudo Genes (multiple problems)  :: 11 of 107
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070179.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1330"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1330"
                     /db_xref="taxon:573"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            join(3948..4062,1..2033)
                     /gene="mobQ"
                     /locus_tag="I6K60_RS26330"
                     /old_locus_tag="I6K60_26330"
     CDS             join(3948..4062,1..2033)
                     /gene="mobQ"
                     /locus_tag="I6K60_RS26330"
                     /old_locus_tag="I6K60_26330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_070749924.1"
                     /translation="MAIYHLSMKIISRSNGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQKEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NHLINRLNLEIQISHTELRNSGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKLEKQDIPASQTAFEKQWQQWGESGLAALRSQL
                     LKREEELRRKELEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQYPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2281..2982)
                     /locus_tag="I6K60_RS26335"
                     /old_locus_tag="I6K60_26335"
     CDS             complement(2281..2982)
                     /locus_tag="I6K60_RS26335"
                     /old_locus_tag="I6K60_26335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281396.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVEITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3515..3769)
                     /gene="mobC"
                     /locus_tag="I6K60_RS26340"
                     /old_locus_tag="I6K60_26340"
     CDS             complement(3515..3769)
                     /gene="mobC"
                     /locus_tag="I6K60_RS26340"
                     /old_locus_tag="I6K60_26340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          2..244
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.361"
                     /coverage="100.0"
                     /evalue="1.19e-117"
     Typing          complement(3771..3947)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          3948..4062
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.69"
                     /coverage="100.0"
                     /evalue="7.92e-45"
     Typing          complement(2283..2982)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..2030
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.353"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ctcacgacta cacccgcaaa agcggtgtgg ctgaggctgt gattctgacc cccgcgacag
       61 cccctgcctg gtgtacgaac cgtgccgaac tctggaatgc cgttgagaag gccgaacgcc
      121 ggaagaactc acaacttgcg cgggaaattg aactcgccat cccccacgaa ctgccgcagg
      181 atgccgcacg ggaaaccgtt ctcgcctttg tcagagaaaa ctttgtcagt cagggcatga
      241 ttgctgatgt ggcgttccat cacatggata aaacaaatcc ccatgcgcac atcatgctga
      301 ccacgagggc tgtcgggcct gctgggttcg gcggaaaggt cagggactgg aacgaccgga
      361 cacatgccga gacgtggcgt gcatcatggg ctgaccatgc aaacagggca ctggcgaacg
      421 ccggttatca gaaagagata gaccatcgtt catacgagcg acaggggctt gagaaaacgc
      481 cgggtattca cctcggaaag tctgcctgtg cgatggaaac acgcggtatc gaaacagaac
      541 gcggcgaaca gaatcatctg attaacaggc ttaacctgga aatacagatt tcccacaccg
      601 aactcaggaa cagcgggctg acagagcctg cccgccgtgt ggctgacctt ctgaatatcg
      661 ggcttcctga caacgcaact tcgtacacac tacgtgacat catcgaagcg ttgccgaagg
      721 acagtaacgc ggcatggaaa ctcaccagta acgccatgtc gatgatggca gatatgcagg
      781 cgacgcgcag acgctgggaa gagctgaaca ccgaacgaaa ggaagcgatg agccatgctg
      841 ccagcctgaa aaaatcatcg ccgttttcgt ccggtttttc ccgtattccc ctgatgcaat
      901 gggctgcacc ggaataccgt agtgagcagg gaaaaatcca gtctctgagc cagcaaatgg
      961 aaaaattgcg acagcattac cggaagctgg agaaacagga cattcccgcg agtcagacgg
     1021 catttgaaaa acagtggcag cagtgggggg aatccggcct tgcagctctc agatcccagt
     1081 tgcttaaacg tgaagaggaa ttgcgccgga aggagctgga ggatacagaa cgtagtcgcg
     1141 cggagcagct caggaaaaat gacaatgccg ttatggattc aggggtgctg agagcagtcg
     1201 ttacggggta tggcgaggct cccatgcctg gtaacgggga aatgacctgt taccttctgc
     1261 ttcacaaccg gaacggagaa tatacgctct gggggaacga gctggagaaa taccgcacca
     1321 ggatctttga gagtgttgat ttaatgcgtg acaggagtgg ttatatctgt gaccgaagcg
     1381 aaatcggtca atatccacca ctgcaacgtg tttattcctc tgcgaccttt gagcaacttc
     1441 tgacgcaggt ttgccagaca tggccgcaac atacccgcaa tctccgccaa ccgaagacat
     1501 ggccggagtc tttctgtctt ggagaagacc gccagccagc catgccatca ctggcagcaa
     1561 ggaaggtgga ttttacgcag ggacgtctgc caccgacgct gatgccggtc atgagttctg
     1621 tcgaccggga aacccgccag cttcagcttt tgctggtcat gggcgtggat gactctctgg
     1681 gcggtgttgt ccggcttaat ggtacgctgt atcccgcgtt tgccgttccc tcagccgata
     1741 acagccagct ggtcatcagt gcactgaccg ataaggggtt acggtatgca gggtatggtg
     1801 ttgccgtaaa tcatgatgca gacagccaca tatcacctgc gccggaactt atggagtttc
     1861 atctgaaaac ccgtgaggca ccgctttttg ctgccgtcaa cacaccagaa aaacagccgg
     1921 atcatctgtt ccgctcgctg ggtttcaatc gtacctggga cgaatggcgg cgcgaggaag
     1981 atgcacgaac acacgccacc gagaggagac atgatcgcgg ttggtcacag tgaatgtgaa
     2041 caatattttg ttttgtttaa catcgtgtta tgttcgtcga tgttcatcga cagggatgaa
     2101 tgcaagaagc acactcacat aacgcaagca tagttttgtt tttccgcccc caaaaattgg
     2161 gggttctttt ttcgctgcat cagagcgttt ttgacgcaat ttttcacttc aatagtacct
     2221 tcgcactact aacacaaaaa aaacgcaaca gggagtgttt taggtgcctt aaaatcgatt
     2281 ctagatcgtc atcttgatct gtttgtcttc cttgaagcga aaatgcaggg ctacaaccgt
     2341 gcgccccttt ttgatcgtct caaccgttac aaccagatcg cttttttcgt tcagttcggc
     2401 gatggctggc ttgattatca tcttattcaa gtctttgaat tgttgatatt tatcttcaac
     2461 ggcaagcatg gttcggaaat catcgatagc tatcgttctg tcaccggtgc tgcggaactg
     2521 ctggagcagc tcgtaaatgc ggatgctgta ggtgctggag aggctggaga cgttcttcac
     2581 gactacccgc gtaaactgcc ccttgagctg tgtcagatac ggcatgatgg catcggagaa
     2641 ggtgatctct actttcgcct cacctttgaa atactgcgcc cggtactgca cccagcggaa
     2701 ctcttctcgt ttttcctcgt tcttgatgat tactgacctg tcccagaggc gatctatcgc
     2761 ttccttgagc ctagcttcag cgtgctgccg tcccatgtca ggaaaggcat catgaaactc
     2821 tgcagccgtg atggtcatgg ttttttgttc atccggcgaa ggggaatctt ctccggattt
     2881 caggcggctg atacagacca gcaggaagcg ctgttcctgc aaagtgagct tgtagcttgc
     2941 ctcgataagg gcgtttgatt tgtaagctat cagatctgac atttcgcttg ctcgttgcta
     3001 ttggtggcgt ttttttactg tacgccaccc atcatattgg tggaagtttt ttttttgcaa
     3061 cccctttttt ctgtggatta cacccccaaa agctccacct atcaccccca aaagctccac
     3121 ctatcacccc caaaagctcc acctatcacc cccaaaagct ccacctatct cgccagaaca
     3181 cctttaaaat catgtggtta caagacctaa aaaggtttta aaaaaaaaga tctaaaaata
     3241 agatcttaaa aacttaacgg cgagcctgtg gataacttgg ggtgctgaaa ataaaaatcc
     3301 aataaaaaca atgtgttgaa aaaaatcagg attgaagatt ccgaatacac cccctccaac
     3361 cctgtggaaa tccagtattc atgcgggttc agcgcatatc acctgtcgaa aaaccgtgcg
     3421 tatgtgctca acatggcgac tgattcactt ccgcttcctc gcccactcgc tcgctaacgc
     3481 tcggtcgctg ggctgcggcg agcggacgat acaatcactg ctctggcagc gcctccagct
     3541 cgttaatcag cggcagtatt cgcgtcacat cagcctgccg tgtcacctct gccttcagga
     3601 actggagtaa ccaagctcgc aactgcactt cctccggctt tctgactttt gccagcaatg
     3661 ctgcacccac aatgaccttc tgcgctgtgt ccaggctccg gctcttcgcc ttcaggcgct
     3721 gtaatctcgc ctcagcatca gcaatcttct gttcgagcgt tctgctcatg atgtaactcc
     3781 atgcgcgatg aaaaatcgca ttttagcgcg tcactggtag tttaaaaact gagctggcat
     3841 aatgcacggc aaatcacgaa gtgcgcactt atacaatctc cactccgttt cgattgtgtg
     3901 cgccctgcgg ggctgaaaga aaactgcaaa aaggcattac ggcagaaatg gcgatttatc
     3961 atctcagtat gaaaatcatc tccagaagca acggctacag cgccgttgct tctgctgcct
     4021 atcgttctgg ttcactaatg cttgatgagc gtaccggact ga
//
