LOCUS       NZ_CP070168             1130 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1283 plasmid unnamed6.
ACCESSION   NZ_CP070168
VERSION     NZ_CP070168.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357425
            Assembly: GCF_016904615.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 868.58x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:33:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,127
            CDSs (total)                      :: 5,003
            Genes (coding)                    :: 4,791
            CDSs (with protein)               :: 4,791
            Genes (RNA)                       :: 124
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 96 of 212
            Pseudo Genes (incomplete)         :: 128 of 212
            Pseudo Genes (internal stop)      :: 49 of 212
            Pseudo Genes (multiple problems)  :: 52 of 212
            Pseudo Genes (short protein)      :: 3 of 212
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070168.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1283"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1283"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            90..1016
                     /locus_tag="I6K13_RS25365"
                     /old_locus_tag="I6K13_25360"
     CDS             90..1016
                     /locus_tag="I6K13_RS25365"
                     /old_locus_tag="I6K13_25360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_204695245.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1019..>1130
                     /locus_tag="I6K13_RS25795"
                     /pseudo=""
     CDS             1019..>1130
                     /locus_tag="I6K13_RS25795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     Typing          91..1013
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.377"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtgctaatt cgtaaaatta gaaggaagcg cacttatatg tttctctcct tcgtcgagaa
       61 acagtgcgct cctgcagagg gctgaaaccg tggcaaccta tcacctcagc gttaaatttg
      121 gtggcaaggg gcaggctgca aaccacgccg attacatcga gcgcaaagag aaataccgcg
      181 accgtcagga tttggaatat tccgcacacg gaaacatgcc ggaatgggcc agggataatc
      241 cttcccattt ctggcaggcg gctgaccagt ttgaacgtgc gaacggttcg acctataggg
      301 aacttgaaat tgcattaccc cgtgaactga caccggaaca gcgccttgaa ctggtgcagg
      361 attttgtacg tcaggaagct ggcgaacggc atgcatggag ttttgccatt cacaatccga
      421 aagcgagcat tgagggaggc gaacagcctc atgcgcatat catgatgagt cagcgtgtga
      481 atgatggcat tgaacgtaca ccggaacagt atttccggcg ctataacgcc aggtatcccg
      541 agcggggagg tgcgaaaaag gattcaggaa gcctgactcc cactcagcaa aaagagcagt
      601 tgcgggaatt gcgaaaacgc tgggaggtga agcacaacga acatatgaga aagcatggct
      661 ttgaaagggg cttcattgac tgccggacgc tgaaagaaca gggcattgaa cgacgtcccg
      721 aagtgcatct gggttttgaa gctgccggac ggcttgatga tgcccagcgt catgacatca
      781 tgcaaaaacg gtctgaaccg gactattccc gccatcttga acaggcgcga acggtcgggc
      841 atgacatcat ggaatggctg gacactccgg ttaagcaaca tgaactggtc agaaggctgc
      901 aacatgatgt tgcgcgggag catggctttg acatgagaga cagagtgagc agacaggacc
      961 gaactgaacc tgaacgggtt aaatcaccgt cacgtggatt ttctatggga ttctgaacat
     1021 ggccagcaca gaagaaaaat tactcacgct cctgttatcg gtggagcatc tgcaacatac
     1081 cgcaatggag caacagaaag ctgttgctga ttctctggct gtggcagggg
//
