LOCUS       NZ_CP070161             1222 bp    DNA     linear   CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1310 plasmid unnamed3.
ACCESSION   NZ_CP070161
VERSION     NZ_CP070161.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357452
            Assembly: GCF_016904595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1222)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1347.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:53:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,433
            CDSs (total)                      :: 5,309
            Genes (coding)                    :: 5,158
            CDSs (with protein)               :: 5,158
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 59 of 151
            Pseudo Genes (incomplete)         :: 92 of 151
            Pseudo Genes (internal stop)      :: 26 of 151
            Pseudo Genes (multiple problems)  :: 22 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070161.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1310"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1310"
                     /db_xref="taxon:573"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            <1..159
                     /locus_tag="I6K40_RS27030"
                     /old_locus_tag="I6K40_27030"
     CDS             <1..159
                     /locus_tag="I6K40_RS27030"
                     /old_locus_tag="I6K40_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_204746433.1"
                     /translation="IGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVRE
                     QGTRDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            368..>799
                     /locus_tag="I6K40_RS27340"
                     /pseudo=""
     CDS             368..>799
                     /locus_tag="I6K40_RS27340"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            837..>1222
                     /locus_tag="I6K40_RS27305"
     CDS             837..>1222
                     /locus_tag="I6K40_RS27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_227640040.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRKQNESL"
                     /name="MbeB family mobilization protein"
     Typing          150..559
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.81"
                     /coverage="99.0"
                     /evalue="8.27e-92"
ORIGIN
        1 atcgggaata acctgaatca gacagcgagg aaggtgaaca gcgggcagtg gtcttccggt
       61 gaccgggttc aggtggtggc cgcactgatg gccatcgggg atgagctgcg ccggctgcgt
      121 ctggccgtca gggaacaggg gacgcgggat gatagttaag tttcatgccc ggggaaaagg
      181 cggtggcagt gggccggttg attacctgct gggaagggag cgaaaccggg aaggggcaac
      241 ggtgcttcag ggtaatccgg aagaagtccg ggaactcatc gatgccacgc catttgcgaa
      301 gaaatacacg tccggtgttc tctcgttcgc ggagaaggcg ctgccagcgg gaggacgtga
      361 aaaagtgatg gcgagctttg agcgtgtact gatgcccggt ctcgagaaaa atcagtacag
      421 catcctgtgg gtggagcacc aggacaaggg acggcttgag ctgaattttg tcattccgaa
      481 catggagcta cagaccggaa aacgcctcca gccgtactac gaccgggcag acaggccgag
      541 aattgatgcc tggcagacgc tggtgaatca ccattacggt ctgcatgacc ccaacgcccc
      601 ggagaaccgc cggacgctga cccttcctga taacctgccg gaaacgaaac aggcgcttgc
      661 tgagggcgtc acacgcggga tagacgcgct ttaccatgcc ggagagataa aaggccgtca
      721 ggatgtgatt caggcgctca tggaagcagg gattgaagtg gtcagggtga cgcgaagcag
      781 tatcagcatt gcagacccga acggcgggaa gaatatcaga ctgaaaggag cattctatga
      841 gcaatctttt acagacgggc gcggagttcg agaaaaagct gaaagagaga gccgaatcta
      901 cagagaaaat gctgaacgac gagttcagga agctcggcga atctgtaagc aaggctgtga
      961 catcaaacga gacgaaaatc aaagacgcta tagccctgtt cacagcttcg acagaggaat
     1021 cactgaaaaa acaccgggaa ggggtgaaag aggcgatgat gcagcacagg aaggacgtgt
     1081 taaagctggc agggaatacg ggcatgatgt tactggggat ggtcattttc ctgtttaccg
     1141 tgagtggcgg gacgctctgg tatcttggcg ggatgataca ggcgaacctg gaagaaatca
     1201 gaaagcagaa cgagagtctg ga
//
