LOCUS       NZ_CP070160             2058 bp    DNA     circular CON 03-DEC-2023
DEFINITION  Klebsiella pneumoniae strain FDAARGOS_1310 plasmid unnamed2,
            complete sequence.
ACCESSION   NZ_CP070160
VERSION     NZ_CP070160.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357452
            Assembly: GCF_016904595.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1347.33x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/03/2023 03:53:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,433
            CDSs (total)                      :: 5,309
            Genes (coding)                    :: 5,158
            CDSs (with protein)               :: 5,158
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 151
            CDSs (without protein)            :: 151
            Pseudo Genes (ambiguous residues) :: 0 of 151
            Pseudo Genes (frameshifted)       :: 59 of 151
            Pseudo Genes (incomplete)         :: 92 of 151
            Pseudo Genes (internal stop)      :: 26 of 151
            Pseudo Genes (multiple problems)  :: 22 of 151
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070160.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1310"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1310"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(join(1487..2058,1..418))
                     /locus_tag="I6K40_RS27020"
                     /old_locus_tag="I6K40_27020"
     CDS             complement(join(1487..2058,1..418))
                     /locus_tag="I6K40_RS27020"
                     /old_locus_tag="I6K40_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_040207214.1"
                     /translation="MQNPELQNLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDSRWMFLTLTVRNCAIEELGETLSRMNTAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPGMLNGKSYVRHERWVELWRECLRVSYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPKWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVKKYRRSPTKDKTESD"
                     /name="protein rep"
     gene            complement(742..933)
                     /locus_tag="I6K40_RS27025"
                     /old_locus_tag="I6K40_27025"
     CDS             complement(742..933)
                     /locus_tag="I6K40_RS27025"
                     /old_locus_tag="I6K40_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020324555.1"
                     /translation="MIVRLDNRLFEILDEMGAHVDREMFMAAYAQRKAEKENDPRHIRQ
                     RKLTRLAHDLMSESDPEL"
                     /name="hypothetical protein"
     Typing          1570..1761
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.446"
                     /coverage="100.0"
                     /evalue="2.86e-91"
ORIGIN
        1 gctggaaagc cgtgttcatc cggctcagcg tctcgcccag ctcctcgatc gcgcagttgc
       61 gcacggtcag ggtcagaaac atccagcggg aatcggggta atccgccacg atacgcggca
      121 gggactggta gaaacgggct tgccacatca gggagcgcct ccactggcag acaggacagt
      181 gccggacacg gcagaaatgc gcctcacgca gcctcaggcg cgtttctccg gtttctttga
      241 gggtagacca cccaaacctc agtaaaccgc cacaggaggc catacgcgcc ccgtagcgct
      301 catattcagc cgcaagcagg taaatccctc ccacgtcgtc agaaacgctt ctgtgagcgt
      361 cccacggcgc atctgagggg gagtagtcag ttaggttctg gagttcagga ttttgcatgc
      421 acaccgcccc atgctgggcg atggctcatt tgacgtgtta tcattgccat atcacctgtg
      481 ttgtcgtgtc caaattcgaa ttcctacagg tggtcacacc cccgaactgt tagcgcagtt
      541 gcgggggtcg cttttttctg cgaccactcc cgacagaata cttccaaaac cattttcagg
      601 caaataacac tcaaaccctt gcggcacaag ggctggcggc gttttttgac gccagagtta
      661 tcccttaagt ataaatacaa ggatcgcgcg cacgagactc cggccatttt ctgaccgatt
      721 tccctcgcgc gcacgcgagg ctcacaattc agggtccgat tctgacatca aatcatgagc
      781 caacctggtc agtttccgtt gacggatatg ccggggatca ttttcttttt cggccttccg
      841 ctgcgcatag gccgccatga acatttcccg atccacatgc gcacccatct catcgagaat
      901 ctcaaacagc cggttatcca gtctgacgat catgcctacc ccctcaaaaa acgcttatca
      961 ggtggttgcg ctcactgcgt gatcacaacc accccctgta agtattttac gggtgctgtc
     1021 gtcaatcact aaatcggaac ccactggcaa gccagcggga ccccgcgatt tagcgtttga
     1081 cgatttcggg aacggcgaac gctgacagat tttaatctgg cggcgtgagg tgttggcagg
     1141 gtgtcggggc gcagccctga cggatcgttt ttggtaccgg catcgtgatg gcggtactcc
     1201 aaaaacacat cttgtccgtt atcagttttt gtttttcgtg ggcttgcccc accgcgcgtt
     1261 tctcatcgcc agatgagaaa ctactaagcg cgccttccgg aaaattaccg gaagaaaaac
     1321 ccaggttcgg aatccgaact cagtaatcga gtgacgtcac tttgcggcgt tagccctatc
     1381 ctgcatcgga gtaaaatcaa tcggacaaga tgtgactttg gtacaccgcc accaaggtga
     1441 cggatgccca aagccctcat ggtcagggcg cagccccgac accctgttag tctgattcgg
     1501 ttttatcttt cgtcggcgaa cgccggtact ttttgacctc ggtcttccac tcgaacgcga
     1561 tgcgggaacc gtcatcatcg ccctcggaaa tatcatcccc gatcaccatg tcggcgtcgg
     1621 tttcctgatc gagtttcagc acgtctttga gtgcgccgcc ggtcgccaca aagcggcgct
     1681 tgtgcgtctg gcgggtcagt tcgaggaacc atttaggatc ggccaccatg tcggccggct
     1741 tcgtggaata tttcagggtt tcggccactg cgccgcgcac cagttcggcg gtcgcgcagg
     1801 ccagcgattc gccgtccttc ggcttgcggg gtttcactgc ccggacatcg acattcggat
     1861 cgtaactgac ccgcaggcac tcacgccaca gctcaaccca gcgttcatgc ctgacgtaac
     1921 tcttcccgtt aagcatgccc ggcggaacca tcatcagggt gtggaagtgc gggtgtgcgc
     1981 tgccgtcact gccgcgggtg acttccgtgg tgcgtatcca gccctgaact ggtcggaact
     2041 ctttacggtc cttcagtc
//
