LOCUS       NZ_CP070156             1161 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1285 plasmid unnamed5.
ACCESSION   NZ_CP070156
VERSION     NZ_CP070156.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357427
            Assembly: GCF_016904555.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1161)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1142.84x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:22:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,977
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 148 of 263
            Pseudo Genes (incomplete)         :: 120 of 263
            Pseudo Genes (internal stop)      :: 33 of 263
            Pseudo Genes (multiple problems)  :: 35 of 263
            Pseudo Genes (short protein)      :: 5 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070156.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1285"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1285"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(292..546)
                     /gene="mobC"
                     /locus_tag="I6K15_RS24595"
                     /old_locus_tag="I6K15_24595"
     CDS             complement(292..546)
                     /gene="mobC"
                     /locus_tag="I6K15_RS24595"
                     /old_locus_tag="I6K15_24595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091747.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            725..>1161
                     /gene="mobQ"
                     /locus_tag="I6K15_RS24600"
                     /old_locus_tag="I6K15_24600"
     CDS             725..>1161
                     /gene="mobQ"
                     /locus_tag="I6K15_RS24600"
                     /old_locus_tag="I6K15_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_204628470.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAALWNAVEKAECRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPA"
                     /name="mobQ"
     Typing          complement(548..724)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          725..1083
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="100.0"
                     /evalue="1.83e-175"
ORIGIN
        1 acaagatctt aaaaacaaga tctaaaaact taacggcgag cctgtggata acttggggcg
       61 ctgaaaacaa aaaaccaata aaaacaatgt gttgcaagaa agcagaattg cacgttccga
      121 atactcccct tccaaccctg tggaaatcca gtattcatgc gggttcagcg catatcacct
      181 accgaaaaac cgtgcgtatg tgctcaacct ggcgacggat tcacttccgc ttcctcgccc
      241 actcgctcgc taacgctcgg tcgctggggc tgcggcgagc ggacgataca atcactgtcc
      301 tggcagtgcc tccagctcgt taatcagcgg cagtatgcgc gtcacatcag cctgtcgtgt
      361 cacctctgcc ttcaggaact ggagtaacca ggcacgcaac tgcacctcct ccggctttct
      421 gacctttgcc agcaatgctg cacccacaac aaccttctgc gctgtgtcca ggctccggct
      481 cttcgccttc aggcgctgta atctcgcctc agcatcagca atcttctgtt cgagcgttct
      541 gctcatgatg taactccgtg cgcgatgaaa aatcgcattt tagcgcgtca ctggtagttt
      601 aaaaactgag ctggcataat gcgcgacaaa tcacaaagtg cgcacttata caatctccac
      661 tccgtttcga ttgtgtgcgc cctgcggggc tgaaagaaaa ctacaaaaag gcattacggc
      721 agaaatggcg atttatcatc tcagcatgaa aattatctcc agaagcagcg gctacagcgc
      781 cgtagcttcc gctgcctatc gttccggttc actgatgctt gatgagcgta ccggactgac
      841 tcacgactac acccgcaaaa gcggtgtggc tgaagctgtg attctgaccc ccgcgacagc
      901 ccctgcctgg tgtacgaacc gtgccgcact ctggaatgcc gttgagaagg ccgaatgccg
      961 gaagaactca caacttgcgc gggaaattga actcgctatc ccccgtgaac tgccgcagga
     1021 tgccgcacgg gaaaccgttc tcgcctttgt cagagaaaac tttgtcagtc agggcatgat
     1081 tgctgatgtg gcgttccatc acatggataa aacaaatccc catgcgcaca tcatgctgac
     1141 cacgagggct gtcggacctg c
//
