LOCUS       NZ_CP070155             1293 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1285 plasmid unnamed4.
ACCESSION   NZ_CP070155
VERSION     NZ_CP070155.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357427
            Assembly: GCF_016904555.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1293)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1293)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1142.84x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:22:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,977
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 148 of 263
            Pseudo Genes (incomplete)         :: 120 of 263
            Pseudo Genes (internal stop)      :: 33 of 263
            Pseudo Genes (multiple problems)  :: 35 of 263
            Pseudo Genes (short protein)      :: 5 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070155.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1285"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1285"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(<1..>1293)
                     /locus_tag="I6K15_RS24590"
                     /old_locus_tag="I6K15_24590"
     CDS             complement(<1..>1293)
                     /locus_tag="I6K15_RS24590"
                     /old_locus_tag="I6K15_24590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_204628469.1"
                     /translation="WADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMET
                     RGIETERGEQNRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRD
                     IIEALPKDSNAAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSG
                     FSRIPLMQWAAPEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGA
                     SGLAALRVQLLKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGE
                     APMPGNGEMTCYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQH
                     PPLQRVYSSATFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVD
                     FTQGRLLPTLMPVRSSVDRETRQLQLLLVMGV"
                     /name="MobA/MobL family protein"
ORIGIN
        1 acgcccatga ccagcaaaag ctgaagctgg cgggtttccc ggtcgacaga actccttacc
       61 ggcatcagcg tcggtagcag acgtccctgc gtaaaatcca ccttccttgc tgccagtgat
      121 ggcatggctg gctggcggtc ttctccaaga cagaaagact ccggccatgt cttcggctgg
      181 cggagattgc gggtatattg cggccatgtc tggcaaacct gcgtcagaag ttgctcaaag
      241 gtcgcagagg aataaacacg ttgcagtggt ggatgttgac cgatttcgct tcggtcacag
      301 atataaccac tcctgtcacg cattaaatca acacgctcaa agattctggt gcggtatttc
      361 tccagctcgt tcccccagag cgtgtattct ccgttccggt tgtgaagcag aaggtaacag
      421 gtcatttccc cgttaccagg catgggggcc tcgccatacc ccgtaacgac tgcgctcagt
      481 acccctgaat ccataacggc attgtcattt ttcctgagct gttccgctcg tcgctccgcg
      541 cgactacgtt ctgtatcctc ctgctccttc cggcgcaatt cctcttcacg tttaagcaac
      601 tggactctga gagctgcaag gccggatgcc ccccactgct gccactgttt ttcaaatgcc
      661 gtctgactcg cgggaatgtc ctgtttctcc accttccggt aatgctgtcg caatttttcc
      721 atttgctggc tcagcgactg gatttttccc tgttcgctac ggtattccgg tgccgcccat
      781 tgcatcaggg gaatacggga aaaaccggac gaaaacggcg atgatttttt caggctggca
      841 gcatggctca tcgcttcctt tcgttcggtg ttcagctctt cccagcgtct gcgcgtcgcc
      901 tgcatatctg ccatcatcga catggcatta ctggtgagtt tccatgccgc attactgtcc
      961 ttcggcaacg cttcgatgat gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcacg
     1021 atattcagaa ggtcagccac acggcgggca ggctctgtca gcccgctgtt cctgagttca
     1081 gtgcgggaaa tctgtatttc caggttaagc ctgttaatca gacgattctg ttcgccgcgt
     1141 tctgtttcga taccgcgtgt ttccatcgca caggcagact ttccgaggtg aataccaggc
     1201 gttttctcaa gcccctgtcg ctcgtatgaa cgatggtcta tctcttcctg ataaccggcg
     1261 ttcgccagtg ctctgtttgc atggtcagcc cat
//
