LOCUS       NZ_CP070153             3004 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1285 plasmid unnamed2.
ACCESSION   NZ_CP070153
VERSION     NZ_CP070153.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357427
            Assembly: GCF_016904555.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1142.84x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:22:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,977
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,599
            CDSs (with protein)               :: 4,599
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 148 of 263
            Pseudo Genes (incomplete)         :: 120 of 263
            Pseudo Genes (internal stop)      :: 33 of 263
            Pseudo Genes (multiple problems)  :: 35 of 263
            Pseudo Genes (short protein)      :: 5 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070153.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1285"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1285"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            75..1001
                     /locus_tag="I6K15_RS24565"
                     /old_locus_tag="I6K15_24565"
     CDS             75..1001
                     /locus_tag="I6K15_RS24565"
                     /old_locus_tag="I6K15_24565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1004..1537
                     /locus_tag="I6K15_RS24570"
                     /old_locus_tag="I6K15_24570"
     CDS             1004..1537
                     /locus_tag="I6K15_RS24570"
                     /old_locus_tag="I6K15_24570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(1603..1812)
                     /locus_tag="I6K15_RS24575"
                     /old_locus_tag="I6K15_24575"
     CDS             complement(1603..1812)
                     /locus_tag="I6K15_RS24575"
                     /old_locus_tag="I6K15_24575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          76..998
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aattagaagg aagcgcactt atatgtttct ctccttcgtc gagaaacagt gcgctcctgc
       61 agagggctga aaccgtggca acctatcacc tcagcgttaa atttggtggc aaggggcagg
      121 ctgcaaacca cgccgattac atcgagcgca aagagaaata ccgcgaccgt caggatttgg
      181 aatattccgc acacggaaac atgccggaat gggccaggga taatccttcc catttctggc
      241 aggcggctga ccagtttgaa cgtgcgaacg gttcgaccta tagggaactt gaaattgcat
      301 taccccgtga actgacaccg gaacagcgcc ttgaactggt gcaggatttt gtacgtcagg
      361 aagctggcga acggcacgcc tggagttttg ccattcacaa tccgaaagcg agcattgacg
      421 gcggcgaaca gcctcatgcg catatcatga tgagtcagcg tgtgaatgat ggcattgaac
      481 gtacaccgga acagtatttc cggcgctata acgccaggta tcccgagcgg ggaggtgcga
      541 aaaaggattc cggaagcctg actctcactc agcaaaaaga gcagttgcgg gaattgcgaa
      601 aacgctggga ggtgaagcac aacgaacata tgagaaagca tggctttgaa aggggcttca
      661 ttgactgccg gacgctgaaa gaacagggca ttgaacgacg tcccgaagtg catctgggtt
      721 ttgaagctgc cggacggctt gatgatgccc agcgtcatga catcatgcaa aaacggtctg
      781 aaccggacta ttcccgccat cttgaacagg cgcgaacggt cgggcatgac atcagggaat
      841 ggctggacac tccggttaag caacatgaac tggtcagaag gctgcaacat gatgttgcgc
      901 gggagcatgg ctttgacatg agagacagag tgagcagaca gaaccgaact gcacctgaac
      961 gggttaaatc accgtcacgt ggattttcta tgggattctg aacatggcca gcacagaaga
     1021 aaaattactc acgctcctgt tatcggtgga gcatctgcaa cataccgcaa tggagcaaca
     1081 gaaagctgtt gctgattctc tggctgtggc aagggaaact cttcgcggat atcgggatat
     1141 ttctgacggt gtggcaaaac aggtcaggga tgaagtcaga aatgaggtgc agaagctgga
     1201 cgtggcagga ctgataggtg agcgcatcag ccaggatttt cagcggctgg aagagtcagt
     1261 cgcaaacatg aaggcaaacg cggatgccct gaacagggat attgcgacaa ttcacagcaa
     1321 tatcgtgcag gaatacaaca cccttaaagg gtatagctgg aaatggctgg gtggggtgtc
     1381 agtggctttt ctggttatgt tctgtgtgct cggatgggga atgaaaacaa tactcgacag
     1441 gaactacaac gccacagcgg cggtgtatca gaaagttgtg acactggaag ataaactggt
     1501 ggagaaacct gtcacgggga agagccggaa aaactgacgc gcttcgcttg tccaacgcct
     1561 cacgacccgc gaaaggattt cgcgaaccgt tcaccgtttt tttcaatatg attctgtctg
     1621 ctttggtcga taaagcagac gatagtattc cagccttcgt atatataacg gtacgtgctc
     1681 atcagtaaga gctggcggta ttgtgttatc agggggaagt tttttcagat actcacgaaa
     1741 tgcctggctt gatgccataa aatctacagt agcatcgata atatgtgggg tgttttcacc
     1801 taattttgcc atgatgataa tccttatatt atcacggttt gtgaatgata gggtgtcact
     1861 attatttatg cgttagtgaa ccagaaaaaa tcccccgttg gcataggggg atatgtaagg
     1921 agaaataact tcgcaggtgt tcttgtttgt ataacgtgaa aagactgcac gcaacttaat
     1981 aagtctgccg tacttacttt cagttttcaa cataaggttt ataacaatgt atggaatgga
     2041 agtgagcttt aaggtttcag ctctaaaaaa taaaatattt attagttggc tggttgttgt
     2101 tcttgttctg tatgtatata aacactatcg acatattatg accgggaaca ttccttaccc
     2161 cctctgcgct cgcagaggtt ttttttgtca ttaattaata atgaactgaa atgcagttcc
     2221 gatacctcca tttgcacatt atccctgaca tatggtaccg gaaccgcatt aattttatgt
     2281 tataagagtt tatttttaat gtccagatgg tttaatagta tttatatgta attagctgta
     2341 tggatagtac tggttttatt gtataacttg tcgttttttt ttattgtttt tgtgtggtgt
     2401 gctaatcttt aagttataaa aaaacccctt ggaatacagg ggcaaaagta aggaaaacaa
     2461 atggatgctc agtttgtgtg acactttaaa ctaatggaac tatagagtgg aggttgttac
     2521 agtcgctttt ataaaacagc aaccagtttt tttcaatagt ctgatgatgg ttgtgacagt
     2581 tttgtgatat tgttcaaaat gtttcttgtt gaatgtttgt tttttggtct attggtgtct
     2641 ggttttattt tttctgctat tacatatcaa gaaatgtgat tggtgtagca agagtggaat
     2701 gcgatgtgga taaactaatt ggttgtcgaa tctaatttac ttagaaaact acacttgttt
     2761 ttgtgtatga atttagtgaa tttgtctgtc tatgtgtttt cgtgcattta taaatccttc
     2821 gtcaggatct taaatttagt gttgaatatg tagggcctgt acggtaatta ttatttagcg
     2881 tagctacttc atcgttgttc gaacacaacc aacaacctgt aattgttgtt tctcttggtt
     2941 ccccactttt gtggggtttt ttatctcttc cgtgcatcgt aaagcgcgga ctccttctgg
     3001 aaag
//
