LOCUS       NZ_CP070140             1713 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1281 plasmid unnamed8.
ACCESSION   NZ_CP070140
VERSION     NZ_CP070140.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357423
            Assembly: GCF_016904495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 973.38x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:16:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,536
            CDSs (total)                      :: 5,406
            Genes (coding)                    :: 5,156
            CDSs (with protein)               :: 5,156
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 97 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 38 of 250
            Pseudo Genes (multiple problems)  :: 49 of 250
            Pseudo Genes (short protein)      :: 4 of 250
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070140.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1281"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1281"
                     /db_xref="taxon:562"
                     /plasmid="unnamed8"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(196..1110)
                     /locus_tag="I6K11_RS27330"
                     /old_locus_tag="I6K11_27375"
     CDS             complement(196..1110)
                     /locus_tag="I6K11_RS27330"
                     /old_locus_tag="I6K11_27375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_204682884.1"
                     /translation="MPNTIHYPHVIPFISQGKINAIKSTFGNNLSDRECYGIYIWSQKA
                     SSAIYPLLQQLEVTLRNSIDKEATKLIGQKWWDNVYTDTSKSKHGDFIHNINKAKKRYE
                     NEFKKKNPSMANTKIIAPHDDIIAHTDFYTWQAVLSDAFHTQSRSEASRALWPRLTYRV
                     LKGLDRSKDEGTARIDFLNELNEIRNYRNRLSHNDCIWIKIHSKNLQSAVETIREKINK
                     IEGLIKTIDPQVHLSLTKRGSFYHAKRICSQKEAELHLGKGIINSTTDEMNTILDQLYA
                     LTADGKLTGVVKRNTNNIAFHKF"
                     /name="Abi family protein"
     Typing          complement(1300..1712)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.444"
                     /coverage="92.0"
                     /evalue="8.33e-180"
ORIGIN
        1 ggaaatagtt tgctcttttt cctgatgatg atgtatcatg tatggcagtg aagccttgag
       61 ctttcgcttt aatgcattgt gaaattttta tagattcaca atgacattaa ggccacgctc
      121 caccgaaagg tgataatggc aagagatttg gatgaggctc gacaggaaac tgtcgggcct
      181 tttctatttt gtgatctaaa acttgtgaaa tgcgatattg ttggtatttc gttttacaac
      241 acccgttagc tttccgtcag cagttagtgc gtatagttga tctagaattg tgttcatctc
      301 atctgttgtg ctattgatta ttcctttgcc tagatgcaat tcggcctctt tttgagaaca
      361 tattcttttg gcgtgataaa aggaaccccg cttcgttaat gatagatgta cctgtgggtc
      421 tatcgttttt atcagtcctt ctattttgtt tattttttct cttattgttt ctactgcaga
      481 ttgcaaattt ttagagtgta tttttatcca tatgcaatca ttatgcgata acctatttct
      541 gtagtttctg atctcattca gctcgtttaa gaaatcaatt ctggctgtgc cttcatcttt
      601 gcttctatct aatcccttca gtactctgta ggtgagcctg ggccataagg ctcttgatgc
      661 ttcagatctt gattgtgtat gaaaagcatc gctcaaaacc gcttgccatg tgtaaaaatc
      721 tgtgtgtgca atgatgtcat catgaggtgc aattattttg gtatttgcca ttgatgggtt
      781 ttttttcttg aattcattct catatctttt ttttgctttg tttatattat gtatgaagtc
      841 accgtgtttg ctttttgatg tgtcggtgta tacattatcc caccattttt ggcctattag
      901 ttttgtcgct tctttatcaa tcgagtttct caaagtaact tcgagttgtt gcagtaatgg
      961 atatatggca gatgaggctt tctggctcca tatatatatc ccgtagcact ccctatcaga
     1021 caggttatta ccaaacgtgc tttttatagc atttattttt ccttgggata taaatggtat
     1081 tacatgaggg taatgaattg tatttggcat atccatctct atcatacact tagtggtgat
     1141 tggatgttgc catacacgta tggatagttg tagagtgtta aacgaaaaaa ccgccttggc
     1201 gggcggtttt tcgaaggttc aaagagttgg cgctctgtga accaaggtaa ctggcttgga
     1261 ggagcgcagt caccaaaatc tgttctttca gtttagcctt aacgggcgca tgacttcaag
     1321 gctaactcct ctaattcggt taccagtggc tgctgccagt ggcgttaagg cgtgccttac
     1381 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1441 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     1501 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     1561 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     1621 ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagattttgt gatgcttgtc
     1681 aggggaggcg gagcctatgg aaaaacggcc tcg
//
