LOCUS       NZ_CP070139             1603 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1281 plasmid unnamed7, complete
            sequence.
ACCESSION   NZ_CP070139
VERSION     NZ_CP070139.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357423
            Assembly: GCF_016904495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 973.38x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:16:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,536
            CDSs (total)                      :: 5,406
            Genes (coding)                    :: 5,156
            CDSs (with protein)               :: 5,156
            Genes (RNA)                       :: 130
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 94
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 250
            CDSs (without protein)            :: 250
            Pseudo Genes (ambiguous residues) :: 0 of 250
            Pseudo Genes (frameshifted)       :: 97 of 250
            Pseudo Genes (incomplete)         :: 168 of 250
            Pseudo Genes (internal stop)      :: 38 of 250
            Pseudo Genes (multiple problems)  :: 49 of 250
            Pseudo Genes (short protein)      :: 4 of 250
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070139.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1281"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1281"
                     /db_xref="taxon:562"
                     /plasmid="unnamed7"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(join(1206..1603,1..109))
                     /locus_tag="I6K11_RS27320"
                     /old_locus_tag="I6K11_27365"
     CDS             complement(join(1206..1603,1..109))
                     /locus_tag="I6K11_RS27320"
                     /old_locus_tag="I6K11_27365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA plasmid replication protein"
                     /protein_id="WP_033806469.1"
                     /translation="MAAQEVITDAQIRQRLLDLEEQNKKLRQELLDERRNTNFTQTYPK
                     GWERIRNLIQANPGAARLYSVLSEHIDGNCGAVVADQQFLADQLSVTTRTIRNWVNFLE
                     ENKCLVRIPIAGKLCAYALNPTEVWKGYNTSKPYAAFLTKTLVNSEGVIKRRLQACFGT
                     SEPNE"
                     /name="RepA plasmid replication protein"
     gene            612..737
                     /locus_tag="I6K11_RS27325"
                     /old_locus_tag="I6K11_27370"
     CDS             612..737
                     /locus_tag="I6K11_RS27325"
                     /old_locus_tag="I6K11_27370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_204682880.1"
                     /translation="MWLPVLFRFLVDSLATLGHRLAASGVTSRMLSVIPLLFFWG"
                     /name="replication protein"
     Typing          complement(495..576)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.3400000000000002e-35"
     Typing          691..951
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.275"
                     /coverage="100.0"
                     /evalue="1.83e-109"
ORIGIN
        1 tgtttctgcg ctcatccaga agctcttgtc gcaatttttt attttgctct tcgagatcca
       61 gaagtcgttg tctaatttga gcatcagtga ttacttcttg agcagccata atacctccca
      121 attaatttcc tcataagcgg aagaatgatt gcaaatttat gttgttttat ttccaacgtc
      181 aatctattca tttcctatta tgggaattaa ccggaaagag cttgtttccg gttaaccgga
      241 acgatcgttt ccggttactt ttcgaaaacc cccataaaat caaccacata gaacttttct
      301 cctcttatct aaaaaaacaa gcccttcagg gcgcgttagg gggtgtcggg gcgcagccat
      361 gacccagtca cgtagcgata gcggagtgta tactggctta ctcctctgca agcctcgtca
      421 gccgtgattt tgcccgattt cgcgctctcc acgttcaaca gctcgatcat gtttttgccg
      481 tccgttcacc gttaaaacgc ctcagaaatc gggacaggat gtgggaatgt cggaccgcgc
      541 acgcgcactc agacattccc cacctggtca gggcttcacc ccgacacccc gcatccgtgc
      601 gtaagtgctc aatgtggctt ccggttctct tccgcttcct cgtcgattcg ctcgctacgc
      661 tcggtcatcg acttgcggcg agcggtgtca cctctcgcat gttgtcggtg atcccgttgc
      721 tgttcttttg gggatgagtc gccagatagc ccctcaaacg cgctcaaaag ctctcagacg
      781 cacgaaacgg gttttagatg cccgccaggg catggaaggc gcgaaaaacg cttacagagg
      841 gtttcaggag gtcaggcgcg ccgatacccc taaccagggg ctttcaggtc gtcaggaaca
      901 gcgtcagccc cctgcaaagc ccatttgcac ggtacaggca gctattcggt tggtaggtgg
      961 tcgtagaccg tcggtatcac agcgataggg ggtctggggt tctccccaga cggtttccgg
     1021 ttgcgctcaa ggcgcagccg aaaccgtggc ggctcggtcg ttctggagat cggggagcaa
     1081 aaacacgcaa gggattttga ggcggcaaaa gagcaatgca ggatagacaa cccgatgtca
     1141 aatgttaaca tttgaatcag attgtccaaa aacggggttt ttgcctgtgc ctgccgcagg
     1201 ctctgttatt cattcggttc tgatgtgcca aaacatgctt gaagtcgacg ctttataacc
     1261 ccctcgctgt taacaagtgt ttttgttaaa aacgcagcgt aaggtttgga tgtgttatag
     1321 cccttccaaa cctcagtagg attcaaagca taggcgcata attttcccgc gataggaatc
     1381 ctaaccaagc atttgttttc ctctaagaaa ttaacccaat tacgtatcgt tcttgttgtc
     1441 acagataact ggtctgctaa aaattgttgg tctgcaacga ctgcaccgca attaccgtca
     1501 atgtgttctg acaggaccga atacaatctt gctgctccag ggttagcttg tatgaggttt
     1561 ctaattcgct cccatccctt agggtaggtt tgagtgaaat tgg
//
