LOCUS       NZ_CP070130             3681 bp    DNA     linear   CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1292 plasmid unnamed6.
ACCESSION   NZ_CP070130
VERSION     NZ_CP070130.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357434
            Assembly: GCF_016904475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1353.32x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:30:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,705
            CDSs (with protein)               :: 4,705
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 71 of 183
            Pseudo Genes (incomplete)         :: 119 of 183
            Pseudo Genes (internal stop)      :: 40 of 183
            Pseudo Genes (multiple problems)  :: 44 of 183
            Pseudo Genes (short protein)      :: 4 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070130.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1292"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1292"
                     /db_xref="taxon:562"
                     /plasmid="unnamed6"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            complement(302..496)
                     /locus_tag="I6K22_RS24720"
                     /old_locus_tag="I6K22_24720"
     CDS             complement(302..496)
                     /locus_tag="I6K22_RS24720"
                     /old_locus_tag="I6K22_24720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041643.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEVENSTEI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            768..1091
                     /gene="mobA"
                     /locus_tag="I6K22_RS24725"
                     /old_locus_tag="I6K22_24725"
     CDS             768..1091
                     /gene="mobA"
                     /locus_tag="I6K22_RS24725"
                     /old_locus_tag="I6K22_24725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            1439..2794
                     /locus_tag="I6K22_RS24730"
                     /old_locus_tag="I6K22_24730"
     CDS             1439..2794
                     /locus_tag="I6K22_RS24730"
                     /old_locus_tag="I6K22_24730"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_000498067.1"
                     /translation="MFVRLTIENFRSVKENFTLDLSASGSNSHLVNHIYKNAEMSVGTL
                     MSAGIYGANASGKSNVLMAFEAIKYIATDSGDLKEGTRIPCYEPYALSLATNESPVRFV
                     ADFYSVEGNRFNYEVKYIKDRIIFESLDYYPSRVKANLFTRDEGDTWETIKFGGHYRGG
                     VKKIPFFPNNSYLAKAGNNAASPDIIKEAYDFFRKGIRHIGLNEKIRISSFGKREEVVS
                     ETAEVLCMVDTGVAGISVREVESDLPIRMDDDIPPEIKEMIEEDYKYRYLFSHATEEGG
                     SVSFPMSRESEGTQKLFEIIPLIRSAFNNSMVVIIDELDNSLHPHIADLIVKLFNDPDV
                     NKKGSQLIFSTHNMQLMTPEKMRRDQIWFCEKNKGASALYSLDDFDKKKVKTTTPYASW
                     YDEGRFGGVPDINYLKVAKFLSGNAMTAIHDIDVDALAGDFFEEFNEDLSND"
                     /name="ATP-binding protein"
     gene            2787..3404
                     /locus_tag="I6K22_RS24735"
                     /old_locus_tag="I6K22_24735"
     CDS             2787..3404
                     /locus_tag="I6K22_RS24735"
                     /old_locus_tag="I6K22_24735"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="WP_000166785.1"
                     /translation="MTKRKGGRNVPRVMYIYCEGEKTEPLYLESYISENSRRTLSVFKI
                     PKTRKNTPEQLVDEAIKKKNSSSTADGDEFWVVYDQEHLTTQSVLCHQRAWNKANRHGI
                     NIAISCVCFELWLLLHFGYTTRSFSSYENLMSDSPFKGLLPNYNKGSSSTYDVLKNRIG
                     KARKNAAQLEIFAKSDNENIHEPYKFPCYTSFHKLLDAIDNF"
                     /name="RloB family protein"
     gene            complement(3556..>3681)
                     /locus_tag="I6K22_RS25115"
     CDS             complement(3556..>3681)
                     /locus_tag="I6K22_RS25115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_251301829.1"
                     /translation="LSGNYRLESNPVRHDLTPLAAATGNRFRGVSLEVMRQLRLN"
                     /name="replication initiation protein"
     Typing          534..759
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="99.0"
                     /evalue="4.9e-75"
     Typing          769..1088
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="4.26e-60"
ORIGIN
        1 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
       61 tatggaaaaa cgcctgcggt gctggcttct tccggtgctt tgctttttgc tcacatgttc
      121 tttccggctt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgac
      181 accgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga agcggaagag
      241 cgccttatgt gacattttct ccttacgctc tgttgtgccg ttcggcatcc tgccctgagc
      301 gttatatctc tgtgctattt tctacttcaa agcgtgtctg tatgctgttc tggagttctt
      361 ccgcgagttc gtgcagcttc tcacacatgg cggcctgttc gtcggcatcg agtgcgtcca
      421 gcttttcgag cagtgttagg ctctggcttt ttatgaatcc cgccatgttg agtacagttt
      481 gctgctgctt gttcatcttt ctgttttctc cgttctgtct gtcatctgcg tcgtgtgatt
      541 atatcgcgca ccacttttcg accgtcttac cgccggtatt ctgccgacgg atatttcagt
      601 cagacaacac tgtcactgcc aaaaaacagc agtgctttgt tggtaattcg aacttgcaga
      661 caggacagga tgtgcaattg ttataccgcg catacatgca cgctattaca attaccctgg
      721 tcagggcttc gccccgacac cctatgtcag atacggagcc atgttttatg acaaaacgaa
      781 gtggaagtaa tacgcgcagg cgggctatca gtcgccctgt tcgtctgacg gcagaagaag
      841 accaggaaat cagaaaaagg gctgctgaat gcggcaagac cgtttctggt tttttacggg
      901 cggcagctct cggtaagaaa gttaattcac tgactgatga ccgggtgctg aaagaagtca
      961 tgcgactggg ggcgttgcag aaaaaactct ttatcgacgg caagcgtgtc ggggacaggg
     1021 agtatgcgga ggtgctgatc gctattacgg agtatcaccg tgccctgtta tccaggctta
     1081 tggcagatta gcttcccgga gagaaaactg tcgaaaacag acggtatgaa caccgtaagc
     1141 tcccaaagtg atcaccattc gctttcatgc atagctatgc agcgagctga aaacgatcct
     1201 gacgcatcct tcctgttttc ccggggtaaa acatctcttt ttgcggtgtc tgcgtcagaa
     1261 tcgcgttcag cgcgtttcag tggtgcgtac aatttatagg ttatgttaaa tgttgtgtgg
     1321 tgaatggtcg tacaagattc tttatgatga tgttgtttca aatttaaagc atgtgctgta
     1381 tcttgtttat tgcttttttt tcgctttttt ggcgaaaaaa ctcatttagg agttgcctat
     1441 gtttgttcgt ttgactattg aaaattttag atccgtaaag gaaaacttta cgcttgattt
     1501 aagtgcgtct ggctcaaata gccatttagt taatcatatt tataaaaacg ccgagatgtc
     1561 tgttggaaca cttatgagtg ctgggattta tggggcgaat gcgtcaggta agtccaacgt
     1621 tctcatggct tttgaggcta ttaaatatat tgctacagat agcggggatc tgaaggaagg
     1681 aactcgaatt ccatgctacg aaccgtatgc cctctcgttg gctacgaatg agtcaccagt
     1741 tcggtttgtg gctgattttt actctgttga ggggaatcgt tttaattatg aggttaaata
     1801 tattaaagat cgaattatat tcgagtcatt ggattactat ccgtctaggg taaaggctaa
     1861 tttgtttact agggatgaag gcgacacatg ggaaacaatt aagtttggtg gtcattatag
     1921 aggtggggtg aaaaaaatac cattcttccc taataactca tatttggcca aggccggtaa
     1981 taatgcggca tctccagata taattaaaga agcttatgac ttttttagga aagggataag
     2041 gcatataggt cttaatgaaa aaataagaat ctcatctttt ggtaaaagag aggaagtagt
     2101 aagtgaaacc gctgaggttc tgtgcatggt tgatacaggt gttgctggaa tatccgtgcg
     2161 agaagtagaa tctgatttac caataagaat ggatgatgat attcctccag aaataaaaga
     2221 aatgatagag gaggattata agtatcgata tttgttttct catgcaactg aggagggcgg
     2281 gagtgtttct tttcctatgt ccagagagtc tgaaggcact cagaagttat ttgaaataat
     2341 acctttaatt agaagtgcat ttaataacag catggttgtg attatcgatg aactagataa
     2401 tagtttgcat ccacatattg cagatctaat tgttaagcta tttaatgatc ctgatgttaa
     2461 caaaaaggga tctcagctaa tattctctac gcataatatg cagttgatga ctcctgaaaa
     2521 aatgagaagg gatcagatat ggttttgcga aaaaaacaaa ggtgcttcgg cattatacag
     2581 tttagatgat tttgataaga aaaaagtaaa aacaacaaca ccttatgcta gttggtatga
     2641 tgagggccgg tttggcggcg ttcctgatat taattatttg aaagtagcta aattcctatc
     2701 aggaaatgct atgactgcta ttcatgatat tgatgttgat gctcttgctg gtgatttttt
     2761 cgaagagttt aacgaggatc tttctaatga ctaaaaggaa gggagggaga aatgttcctc
     2821 gagtgatgta tatatattgt gaaggggaga agacggagcc gttgtatctt gaatcttata
     2881 tatctgaaaa ttcaagaaga acgttgagtg tgtttaaaat acctaaaaca aggaaaaata
     2941 caccagagca gctagtagat gaggcgataa agaaaaagaa ttcatcatcc actgcggatg
     3001 gtgatgagtt ttgggtcgtt tatgatcaag agcatttgac tacgcagtct gttttgtgcc
     3061 atcaaagggc gtggaacaaa gctaatagac acggtataaa tatagctata tcttgtgttt
     3121 gttttgaact gtggttgttg ttgcactttg gttatacaac aagatctttt tcatcttatg
     3181 aaaatttaat gtctgactca ccgtttaagg gtctgttgcc aaattataat aaaggtagta
     3241 gttcaacata tgatgttttg aagaatagga ttgggaaggc gagaaaaaat gcggcgcaac
     3301 tagagatttt cgctaaatca gataacgaaa atatacatga gccatataaa tttccatgtt
     3361 atacatcatt tcataagtta cttgatgcga ttgataactt ttgaattaat ccattgacca
     3421 tggcttattg ctggttgtaa tctatagaaa ataaaaacca ccgctaccaa cggtggtttt
     3481 tagaaggtac atgagctacc aacttatgta ccgggtaact ggcttagagg agcgcagtca
     3541 ccaaaacttg tccttttagt ttagccttaa ctggcgcatg acttcaagac taactcctct
     3601 aaatcggtta ccagtggctg ctgccagtgg cgttaagtcg tgtcttaccg ggttggactc
     3661 aagacgatag ttaccggata a
//
