LOCUS       NZ_CP070126             6648 bp    DNA     circular CON 17-JUN-2023
DEFINITION  Escherichia coli strain FDAARGOS_1292 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP070126
VERSION     NZ_CP070126.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN16357434
            Assembly: GCF_016904475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6648)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     FDA dAtabase for Regulatory Grade micrObial Sequences (FDA-ARGOS):
            Supporting development and validation of Infectious Disease Dx tests
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6648)
  AUTHORS   Sokurenko,E., Tallon,L., Sadzewicz,L., Zhao,X., Boylan,J., Ott,S.,
            Bowen,H., Vavikolanu,K., Mehta,A., Aluvathingal,J., Nadendla,S.,
            Yan,Y. and Sichtig,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-FEB-2021) Institute for Genome Sciences, University of
            Maryland School of Medicine, BioPark II, 801 W. Baltimore St., Suite
            619, Baltimore, MD 21201, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRT v. 8.0.0, HGAP v. 4
            Genome Coverage                   :: 1353.32x
            Sequencing Technology             :: Pacbio; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/17/2023 05:30:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,003
            CDSs (total)                      :: 4,888
            Genes (coding)                    :: 4,705
            CDSs (with protein)               :: 4,705
            Genes (RNA)                       :: 115
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 183
            CDSs (without protein)            :: 183
            Pseudo Genes (ambiguous residues) :: 0 of 183
            Pseudo Genes (frameshifted)       :: 71 of 183
            Pseudo Genes (incomplete)         :: 119 of 183
            Pseudo Genes (internal stop)      :: 40 of 183
            Pseudo Genes (multiple problems)  :: 44 of 183
            Pseudo Genes (short protein)      :: 4 of 183
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP070126.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FDAARGOS_1292"
                     /host="Homo sapiens"
                     /culture_collection="FDA:FDAARGOS_1292"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="USA: WA"
                     /collected_by="ARMADA"
     gene            152..289
                     /locus_tag="I6K22_RS24610"
                     /old_locus_tag="I6K22_24610"
     CDS             152..289
                     /locus_tag="I6K22_RS24610"
                     /old_locus_tag="I6K22_24610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(1316..1507)
                     /locus_tag="I6K22_RS24615"
                     /old_locus_tag="I6K22_24615"
     CDS             complement(1316..1507)
                     /locus_tag="I6K22_RS24615"
                     /old_locus_tag="I6K22_24615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1571..1894
                     /locus_tag="I6K22_RS24620"
                     /old_locus_tag="I6K22_24620"
     CDS             1571..1894
                     /locus_tag="I6K22_RS24620"
                     /old_locus_tag="I6K22_24620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2103..>2534
                     /locus_tag="I6K22_RS25095"
                     /pseudo=""
     CDS             2103..>2534
                     /locus_tag="I6K22_RS25095"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2572..3090
                     /locus_tag="I6K22_RS24630"
                     /old_locus_tag="I6K22_24630"
     CDS             2572..3090
                     /locus_tag="I6K22_RS24630"
                     /old_locus_tag="I6K22_24630"
                     /inference="COORDINATES: protein motif:HMM:NF016710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            3097..3330
                     /locus_tag="I6K22_RS24635"
                     /old_locus_tag="I6K22_24635"
     CDS             3097..3330
                     /locus_tag="I6K22_RS24635"
                     /old_locus_tag="I6K22_24635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3648..4067)
                     /locus_tag="I6K22_RS24640"
                     /old_locus_tag="I6K22_24640"
     CDS             complement(3648..4067)
                     /locus_tag="I6K22_RS24640"
                     /old_locus_tag="I6K22_24640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4148..4585)
                     /locus_tag="I6K22_RS24645"
                     /old_locus_tag="I6K22_24645"
     CDS             complement(4148..4585)
                     /locus_tag="I6K22_RS24645"
                     /old_locus_tag="I6K22_24645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            4846..6414
                     /gene="cea"
                     /locus_tag="I6K22_RS24650"
                     /old_locus_tag="I6K22_24650"
     CDS             4846..6414
                     /gene="cea"
                     /locus_tag="I6K22_RS24650"
                     /old_locus_tag="I6K22_24650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     Typing          1144..1231
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(575..1130)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          1885..3434
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acaaggaaat aatacccttc gctgtttttt gttataaggt atatatatat aagtgtgcag
       61 tacaggccaa ataaaatatt ttttatgtag tatcttaagc tcataaatta aacctcgcca
      121 tatattcttt tcattttata aggatcgagt tatgaggaaa agattttttg tgggaatatt
      181 cgcgataaac ctccttgttg gatgtcaggc taactatata cgtgatgttc agggagggac
      241 catcgcacca tcctcctctt ctaaactgac ggggatcgcg gttcagtaga aaagatcaaa
      301 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      361 ccgctaccaa cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      421 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagtcgggc
      481 cactacttca agaactctgt agcaccgttt gtgccatcat cgctctgcta atccggttac
      541 cagtggctgc tgccagtggc gttaaggcgt gccttaccgg gttggactca agacgatagt
      601 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      661 agcgaacgac ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc
      721 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
      781 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
      841 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
      901 acgcctgcta cgtggccttc ttcctgttcc tggtcttttg ctcacatgtt ctttccggcc
      961 ttatcccctg attctgtgga taactgtgtt accgtttttg tgtgagtcag taccgctcgc
     1021 cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaaaa gcgcctggac
     1081 gtgcattttc tccttacgca tctgtgcggc atttcacacc cggcatggcg tacttttcat
     1141 acaatccgca ctgatgccgc atggttaagc cagtatacac tccgctatcg ctacgtgact
     1201 gggtcagggc tgcgccccga cacccgctaa aacctgctga cgcgccctga cgggcttgtc
     1261 agctcccggc atccgctcac agacaagctg tgaccgtctc cgggagctgc atgtatcaga
     1321 ggttttcacc gtcatccccg aaacgtgcga ggcagctgcg gtaaagctca tcggcgtggt
     1381 cgtgaagcga ttcacaaata tcggcctgtt catctgcgtc cagttcgttg agcttctcca
     1441 gcagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt
     1501 tagtcactga tgcctccgtg taagggggat ttctgttcat ggggtaatga taccgatgaa
     1561 acgcgagagg atgctcacaa tacgggttac tgatgatgaa catgcccggt tacttgaacg
     1621 ctgtgagggt aaacaactgg cggtatggat gcggcgggtc tgcctggggg agccggttgc
     1681 ccgttccgga aaactgccga cactggcacc gccgttactg cgtcagctgg ccgccatcgg
     1741 aaataacctg aatcagacag cccgtaaggt gaacagcggg cagtggtctt ccggtgaccg
     1801 ggttcaggtg gtggccgcac tgatggccat cggggatgag ctgcgccggc tgcgtctggc
     1861 cgtcagggaa cagggggcgc gggatgatag ttaaatttca tgccagggga aaaggtggtg
     1921 gcagtggtcc ggttgattac ctgctgggga gggagcgtaa ccgcgaaggc gcaacggtgc
     1981 ttcagggtaa tccggaagaa gtccgggaac tcatcgatgc cacgccattt gcgaagaaat
     2041 acacgtccgg tgttctgtcg ttcgcggaga aggagctgcc gccgggagga cgtgaaaaag
     2101 tgatggcgag ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag tacagcatcc
     2161 tgtgggtgga gcaccaggac aagggacggc ttgagctgaa ttttgtcatt ccgaacatgg
     2221 agctacagac cggaaaacgc ctccagccgt actacgaccg cgcagacagg cctagaattg
     2281 atgcctggca gacgctggtg aatcaccatt acgggctgca tgacccgaac gccccggaga
     2341 accgcaggac gctgacactc cctgataacc tgcctgaaac gaaacaggcg cttgctgagg
     2401 gcgtcacgcg aggtatagat gcactttacc atgccggaga gataaaaggc cgtcaggatg
     2461 tgattcaggc gctcactgag gcggggctgg aagtggtcag ggtgacgcga agcagtatca
     2521 gcattgcaga tccgaacggc gggaagaata tcaggctgaa aggagcattt tatgagcaat
     2581 cttttgcaga cgggcgcgga gttcgagaaa aagctgaaag agagagccga atctacagag
     2641 aaaatgctga acaacgagtt caggaggctc ggcgaatctg taagcgaggc tgtgacatca
     2701 aacgagacga aaatcagaga cgctatagcc ctgttcacag cctcgacaga ggaatcgctg
     2761 gaaaaacacc gggaaggggt gaaagaggcg atgatgcagc acaggaggga cgtgttaaag
     2821 ctggcaggga atacgggcat gatgttactg gggatagtct ttctcctgtt taccgcgagt
     2881 ggcgggacgc tctggtatct tggagggagg atacaggcga acctggaaga aatcaggaag
     2941 caggaagaga cattgcagaa actgaacgcg aagacatggg gcgtggagtt tgtgcaggac
     3001 gggaacagga aattccttgt ccttccgtac gggaaatcag cggaggtgat tccctttcag
     3061 gggaaagagt gggtacatct gaaggagtga cacagagtga cagagctgga aacacatttg
     3121 ctgaacgcct tagagcagct gcaacaggac tatatgcagc ggctgagcga atgggagagc
     3181 gccttcgtgg aattgcagaa gatgttttcg cttacgcaac gggacaacgc gatgctgaac
     3241 gagcgggtca tgcagttgag tcagcaggtg cagcacttga gcgagcagac agaacgcttg
     3301 agccagttat acagcgagaa ctggagataa gagaggaacg gctgatacag gagcgcgaac
     3361 atgtgttatc cctggaacgg gagcgtcagc cggaaataca ggaacgcacg ctggatggcc
     3421 cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag tgggggtaat
     3481 gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc aggcgcgatc
     3541 gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt gatcgcgctg
     3601 aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta ctgtctgccc
     3661 tcgtagccat cgagataaac cgcagaaatc gtgtcagcca gcagcctgga ttttttcggg
     3721 gtaaggtctt tgctgcggta atcagtaccc gcaaattgag ttgtgccccg gagtgagttt
     3781 aaaaattcct ggctgtgaaa ctgctgaatg cgttgttgca catcactccg tgattttcct
     3841 gtcgctttat ctttagctcc catatcccag aactgccgaa aaaaaggcac catcattcgt
     3901 gttgtgtcgg ctttttgggt ggaaaagttg ggatcaaaac cgtcatcaga agcatatacg
     3961 taatgccggg tatgttgttc cacgctgccc tttggggtac aggctgtgag agctactgtt
     4021 gctgttatca tggcgataag acaaagtttg tttccggtac gtctcatgag tgtctcccta
     4081 ccttaaagta tttattctcg gtgagttatt atccgaagac tattttatca tcagtctctg
     4141 acagcgttca gcgaccgaac aacctggacc agaatccccg tttcttttcc agttccagtg
     4201 cctgcctgag ctgggctatc tcattttgta gctgttcccg ttctgcttcc tggcgtctgc
     4261 gatccatatc ctgtgcctgt ttatcctcaa gcatcagcgt caggcattgt ttcagctcat
     4321 tcagctcccg gagaatgcgt tctgtctgct gatcatgtgg attttctgca tgtccctcac
     4381 tgtgcctttc tggtgtctca ttctgcttta attcgccgta tgcccggatc agttcactgg
     4441 tttcaaactc ccgtcgtccg tccctgccgg tacggtaact gacaaggcct gcgcgcatat
     4501 cacggtaaaa ctgtgaacgc gatctgcctg tcatttttag tgcgtcccgg agtgtgtgcc
     4561 atgccataaa gtgacagtgt cccatagatg tctcatctca tagtttcagt aaaacataat
     4621 gaggtctgag aacggtaatg tttgtgctgg tttttgtggc atcgggcgag aatagcgcgt
     4681 ggtgtgaaag actgtttttt tgatcgtttt cacaaaaatg gaagtccaca gtcttgacag
     4741 ggaaaatgca gcggcgtagc tttttatgct gtatataaaa ccagtggtta tatgtacagt
     4801 atttattttt aacttattgt tttaaaagtc aaagaggatt ttataatgga aaccgcggta
     4861 gcgtactata aagatggtgt tccttatgat gataagggac aggtaattat tactcttttg
     4921 aatggtactc ctgacgggag tggctctggc ggcggaggtg gaaaaggagg cagtaaaagt
     4981 gaaagttctg cagctattca tgcaactgct aaatggtcta ctgctcaatt aaagaaaaca
     5041 caggcagagc aggctgcccg ggcaaaagct gcagcggaag cacaggcgaa agcaaaggca
     5101 aacagggatg cgctgactca gcgcctgaag gatatcgtga atgaggctct tcgtcacaat
     5161 gcctcacgta cgccttcagc aacagagctt gctcatgcta ataatgcagc tatgcaggcg
     5221 gaagcagagc gtttgcgcct tgcgaaagca gaagaaaaag cccgtaaaga agcggaagca
     5281 gcagaaaagg cttttcagga agcagaacaa cgacgtaaag agattgaacg ggagaaggct
     5341 gaaacagaac gccagttgaa actggctgaa gctgaagaga aacgactggc tgcattgagt
     5401 gaagaagcta aagctgttga gatcgcccaa aaaaaacttt ctgctgcaca atctgaagtg
     5461 gtgaaaatgg atggagagat taagactctc aattctcgtt taagctccag tatccatgcc
     5521 cgtgatgcag aaatgaaaac gctcgctgga aaacgaaatg aactggctca ggcatccgct
     5581 aaatataaag aactggatga gctggtcaaa aaactatcac caagagccaa tgatccgctt
     5641 cagaaccgtc ctttttttga agcaaccaga cgacgggttg gggccggtaa gattagagaa
     5701 gaaaaacaaa aacaggtaac agcatcagaa acacgtatta accggataaa tgctgatata
     5761 actcagatcc agaaggctat ttctcaggtc agtaataatc gtaatgccgg tatcgctcgt
     5821 gttcatgaag ctgaagaaaa tttgaaaaaa gcacagaata atctccttaa ttcacagatt
     5881 aaggatgctg ttgatgcaac agttagcttt tatcaaacgc tgactgaaaa atatggtgaa
     5941 aaatattcga aaatggcaca ggaacttgct gataagtcta aaggtaagaa aatcggcaat
     6001 gtgaatgaag ctctcgctgc ttttgaaaaa tacaaggatg ttttaaataa gaaattcagc
     6061 aaagccgatc gtgatgctat ttttaatgcg ttggcatcgg tgaagtatga tgactgggct
     6121 aaacatttag atcagtttgc caagtacttg aagattacgg ggcatgtttc ttttggatat
     6181 gatgtggtat ctgatatcct aaaaattaag gatacaggtg actggaagcc actatttctt
     6241 acattagaga agaaagctgc agatgcaggg gtgagttatg ttgttgcttt actttttagc
     6301 ttgcttgctg gaactacatt aggtatttgg ggtattgcta ttgttacagg aattctatgc
     6361 tcctatattg ataagaataa acttaatact ataaatgagg tgttagggat ttaattattc
     6421 tttattgata taaaaagtcc tagcaatcca aatgggattg ctaggaccaa acaaagtaga
     6481 ttatatagca taaataggtt taattttgct acgggggcgt tatttaggtt ttttcttctt
     6541 tcgaaaaaat ctttctttat gaagttaaaa gctatgtgtt caatagcata ttttgaatat
     6601 ggacatagaa tagtgcttat cactattgca tatagcatct tatctgac
//
